SK852012A3 - A process for obtaining high-purity beta-1, 3/1,6-D-glucan - Google Patents

Abstract

The method consists in disintegrating and pulping the oyster mushroom associated with extraction in an aqueous medium with a content of 0.10 to 0.60% by weight. of at least one of alkali metal hydroxides, carbonates and bicarbonates, ammonium oxalate and fortifying admixture, at pH = 7.9 to 8.2, followed by extraction with aqueous sodium and / or potassium hydroxide solution and after washing and removal of the water-soluble components, the suspension is washed with dilute acidic aqueous solution to pH 4.8-5.2. Subsequently, the product is bleached for 3 to 26 hours with aqueous solutions of at least two bleaching agents. The ß-D-glucan obtained is washed with water, acidified with acetic acid and / or peracetic acid and finally with distilled water, and dried at a temperature below 65 ° C. It is further treated by sharing, micronizing and gelling.

Description

The invention relates to a process for obtaining high purity beta-1,3 / 1,6-D-glucan (hereinafter β-D-glucan) mainly from oyster mushroom (Pleurotus ostreatus), preferably from its depths by disintegration, defibrating, extraction and efficient bleaching. available bleaching agents, the ⁴ achieves a high efficiency of destruction of the chitin-glucan complex, protein and high purity of the obtained product.

BACKGROUND OF THE INVENTION β-D-Glucan, which is essentially a homopolymer of glucose with a linear molecule, respectively. a macromolecule containing (1,3) β-D-glucoside linkages, optionally with a branched molecule comprising additionally a side chain bound by (1,6) β-D-glucoside linkages. Although chemically a rather heterogeneous group, these polysaccharides are usually referred to by the common name glucans. β-Glucans attract considerable attention as they belong to a group of physiologically active substances, collectively referred to as biological response modifiers. Thus, basically any substance can be a biological response modifier [Palisa V., Holan Z .: Prakt. Medicine. 69, 770 (1989); Novák M .: Chem. Letters 101, 872-880 (2007)], which stimulates an increase in the number of effector cells of the immune system. A greater number of polysaccharides with an immunomodulatory effect are known, among which available and richer sources include mainly oyster (Pleurotus ostreatus) and a number of others such as Laminaria sp., Poria cocos, Grifola frondosa, Sacharomyces cerevisiae and Lentinus edodes and others [Black W.A. P. et al., J. Appl. Chem. 1951, 505; Harada T et al. Biochem. Biophys. 124, 292 (1968); Bartnicki-Garcia S .: Ann. Rev. Microbiol. 22, 87 (1968); Bohn, J. A., Bemiller, J. N., Carbohydr. Polymer 28, 3 (1995); Chichara G. et al: Nature 222,687 (1969) and Cancer Res. 30,2776 (1981); US 3,943,247],

Thus, immunopharmacologically active substances, including β-D-glucanes acting on the host's basic defense systems, significantly increase its resistance to disease, suppress the effects of stress and allergies, accelerate healing and increase resistance to dangerous bacteria and viruses, as well as in treatment cancer, inhibition of metastasis [Patchen M .: Suv. Immunol. Res. 2, 237 (1983); Pospisil et al: Experiencia 38, 1232 (1982)]. In combination with other nutritional supplements, it is effective in lowering cholesterol and triglycerides, increasing the resistance in the treatment of skin against skin diseases, while in cancer therapy the dosage of β-D-glucan is at least 1 mg / kg bodyweight per day (CS 243 173).

Known is yeast β-D-glucan, isolated from the cell walls of baker's yeast by a multi-stage technology and with very low yields. Similarly, the isolation of β-D-glucan from cereals (US 6,323,338; US 6,749,885) and, moreover, the technology of extraction and refining is technically demanding and the yields obtained are low. Similar is the situation of recovering β-D-glucan from other raw materials (e.g. US 6,444,448, US 6,485,945, US 6,197,952), with significantly higher purity, in particular substantially lower protein residue content, of chitin glucan complexes required for significant applications. separately [Speváček J., Synytsa A., Bras J., Ederová J., Čopíková J .: Personal Announcements].

Of interest is an efficient procedure for the isolation of β-D-glucan by extraction from the fungus Lentinus edodes [Yap, Nog. Int. J. Med. Mushrooms 3, 6-19 (2001)] through precipitation in ethanol and freeze-drying in liquid nitrogen, which is probably an even more effective way than previously known procedures [Chichara et al: Cancer Res. 30, 2776-81 (1970); Mizumo: Int. J. Med. Mushrooms 1,

9-29 (1999)].

However, it has proved to be a suitable raw material for obtaining β-D-glucan of oyster mushroom (Pleurotus ostreatus), which yields a high yield of dry matter (Kuniak L. et al: CS 274 918, CS 276 192), which in principle is true. other patents also show technical differences (eg Gabrižová L .: SK 282 870, Doboly T., Dobolyová L: SK 285 062). These are also technically interesting processes for isolation of β-D-glucan, good yields, but an even higher purity of the product is required, without significantly increasing the time required for purification of β-D-glucan. Although purification by water-peroxide, resp. an aqueous-peroxide-alkaline aqueous solution, even a method for purifying yeast β-D-glucan (Masler L. et al: CS 274 030) from undesired α-D-glucan by enzymes with amylase activity, but this method is not preferred for more efficient purification β-D-glucan isolated from oyster mushroom.

These publications and patents only partially describe the method of obtaining β-D-glucan, as well as the other 109 cited papers and publications [Novák M .: Chem. Letters 101, 1872-1880 (2007)., Rastal R. A., Martin V .: Biotetchnology 13, 490-496 (2002); Gibson, R.A .: Probit Nutr. Res. Rev. 17, 259-275 (2004); Synytsya A., Mickova K., Synytsya A., Jablonsky I., Spevacek J., Erban V., Kovarikova E., Copikova J .: Personal Announcements] also provide technically valuable information, but the problem of obtaining high-purity β- D-glucan. Thus, the known scientific and technical information utilizes and still persists the problems of the present invention.

SUMMARY OF THE INVENTION

SUMMARY OF THE INVENTION The present invention provides a process for obtaining high purity beta-1,3 / 1,6-D-glucan from Pleurotus ostreatus, preferably after washing with clear water and its depths, after washing with water, disintegration, conjugated to defibrate in an aqueous environment % containing 0.10 to 0.60 wt. at least one reagent selected from alkali metal carbonates, ammonium oxalate and a fortifying additive, wherein the pH of the aqueous suspension solution is 7.9 to 8.2, and after separation of the suspension, it is still extracted with an aqueous solution of sodium and / or potassium hydroxide at 0 % To 0.5 wt. and after washing, with removal of the water-soluble components, a suspension of mainly beta-1,3 / 1,6-D-glucan containing sodium hydroxide at a concentration of 0.05 to 0.2 wt. and after separating the suspension and washing, removing the water-soluble components, the suspension is mainly beta-1,3 / 1,6-D-glucan separated and after washing with a dilute aqueous carboxylic acid solution to pH 4.8-5.2 and washed with clean water , the insoluble "crude" beta-1,3 / 1,6-D-glucan product is bleached with hydrogen peroxide and at least one other oxidant, at a temperature of 6 to 36 ° C, for 3 to 26 h in an alkali metal hydroxide and / or carbonate environment , followed by dehydration with an organic water-soluble atoxic solvent or lyophilization, with dewatering to dryness and final treatment, wherein the insoluble beta-1,3 / 1,6-D-glucan is defibrated predominantly in an aqueous solution suspension of at least one hydroxide and / or carbonate % to alkali metal bicarbonate, additionally containing 0.03 to 0.15 wt. ammonium oxalate, and a fortifying agent formed by an alkali metal salt of peroxy formate and / or peroxyacetic acid in an amount of 0.1 to 0.45% by weight, after which it is subsequently washed with water and the water-soluble components are washed off, the agglomerated suspension, in particular beta1,3 (1,6-D-glucan) subsequently bleached at a temperature of 6 to 36 ° C for 3 to 26 hours by treatment with aqueous solutions of at least two bleaching agents selected from chlorine dioxide, chlorine dioxide hexahydrate in an amount of 1.5 to 12% by weight, hydrogen peroxide, alkali metal peroxide, peroxy formic acid and its alkali salt, peroxyacetic acid and its alkali salt, in an amount of 2 to 35% by weight, calculated on the dry weight of the suspension and the resulting beta-1,3 / 1,6-D-glucan obtained is washed with water, acidified with acetic acid and / or peracetic acid to a content below 0.25% by weight. and finally washed with demineralized or distilled water and dried at a temperature below 65 ° C.

In particular, the advantages of the process according to the invention are high yields of beta-1,3 / 1,6-D-glucan, in particular high purity, technical availability of active oxidants and other components necessary for efficient isolation and purification. Oxidants are used not only for purification-bleaching but also for the efficient decomposition of undesirable beta-1,3 / 1,6-D-glucan complexes with chitin and proteins at all. Last but not least, the advantage is also the mechanical and technological availability, low energy consumption, and even for a large number of prepared reaction solutions, washing the "cake" of the suspension, especially β-D-glucan, it is sufficient with demineralized or even hygienically available drinking water. Only the final washing should be conducted with demineralized to distilled water.

The fortifying admixture in the aqueous environment is 0.05-0.25 wt. an alkali metal salt of peroxy formic and / or peroxyacetic acid.

The bleaching of the beta-1,3 / 1,6-D-glucan suspension is carried out with chlorine dioxide, preferably in the form of chlorine hexahydrate, in an amount of 2 to 8% by weight, calculated on the dry weight of the beta-1,3 / 1,6 suspension. -D-glucan. The co-bleaching is carried out with hydrogen peroxide and / or an alkaline salt thereof, selected from sodium peroxide and potassium peroxide in the form of an aqueous solution having a concentration of 2 to 25% by weight. and in an amount of 10 to 35% by weight, calculated on the dry weight.

The additional co-bleaching agent may be selected from peroxy formic acid and peroxyacetic acid and / or their alkali salts, in an amount of 3 to 15% by weight, based on the dry weight of the suspension.

The beta-1,3 / 1,6-D-glucan is washed with acetic acid and / or peracetic acid at a concentration below 0.2% by weight prior to final washing with demineralized or distilled water.

The dried beta-1,3 / 1,6-D-glucan is still formulated into a usable form, such as by bleaching, micronization, gelling and optionally formulation with other substances to produce nutritional and therapeutic, especially immunostimulatory, human and nutritional products. animals.

Further specific data as well as other advantages of the process of the present invention are apparent from the examples.

DETAILED DESCRIPTION OF THE INVENTION

Example 1

In the laboratory disintegrator-mixer, 5 kg of Pleurotus ostreatus are defibrated 12 h after harvesting in an environment of 8 kg of 0.15 wt.% Aqueous sodium carbonate solution. for 3.5 min, resulting in a relatively homogeneous suspension in water, with the pH of the environment reaching 7.9 to 8.1. After defibrating, the reaction mixture is filtered on the filter, thoroughly washed with drinking water and further centrifuged to remove water-soluble components such as carbohydrates, proteins, enzymes, ash, as well as water-soluble glucan. The insoluble portion of the glucan thus obtained is completely freed of the liquid phase and fed into a reactor of 15 dm ³ volume and 60 g of a 29% w / w aqueous sodium hydroxide solution are poured. % and 1 wt. sodium carbonate. The resulting mixture is stirred vigorously and 430 g of 30% w / w hydrogen peroxide are gradually added thereto for bleaching. and then allowed to bleach at 20-25 ° C for 16 h. After bleaching, the obtained glucan is thoroughly washed with drinking water until the violet-red color of the phenolphthalein indicator disappears. Thereafter, 1 kg of slightly acidified drinking water, containing 0.005% by weight, is added to the obtained filter cake. acetic acid and then, after thorough mixing, the filter cake is washed again with 2.5 kg of demineralized water. The resulting wet glucan is then efficiently compressed and thus pressed cake in a reactor of 4 kg of concentrated ethanol and allowed to stand for 1 h. Thereafter, the suspension is again put into a press and then pressed. Such dehydration of the cake with ethanol is repeated two more times, after which the obtained glucan is dried at 50-55 ° C under slightly reduced pressure.

The yield of the obtained glucan per weight of oysters of oyster mushrooms reaches 71%, being insoluble not only in water but also in dilute acids and organic solvents, it contains 0.72% by weight. % chitin nitrogen, 1.57 wt. ash, easy to grind to a grain size of 0.2 to 0.5 mm.

Example 2

The procedure is analogous to Example 1, except that the depths are first rinsed with drinking water, then in an autoclave in a saline solution at a concentration of 8.5% by weight. NaCl, then rinsed with demineralized water at 30-38 ° C before disintegrating and defibrating 5 kg oysters in 8 kg aqueous sodium carbonate solution at a concentration of 0.15% by weight. moreover, it is fortified with 0.28 wt. potassium percarbonate.

To 15 dm ³ reactor sequentially in addition to the bleaching of 430 kg of hydrogen peroxide of a concentration of 30% by weight. 50 g of chlorine dioxide hexahydrate are added.

The yield of the obtained glucan per oyster mushroom dry weight was 73.5%, containing only 0.38% by weight. % of chitin nitrogen and 1.17 wt. ash, it is also easy to grind to a grain size of 0.15-0.4 mm and is suitable for the preparation of gels.

Example 3

The procedure is similar to that of Example 2, except that a 0.2 wt. % sodium peroxyacetic acid and 0.1 wt. potassium peroxy formate.

The yield of β-D-glucan is 74.2%, containing 0.21% by weight of chitin nitrogen and 0.97% by weight. ash. High purity is suitable for the formulation of gels with therapeutic effect also on the skin.

Example 4

The procedure is analogous to Example 2, with 0.2 wt. sodium peroxy formate, only 8 kg of an aqueous solution of sodium carbonate at a concentration of 0.15% by weight. % as a co-bleach instead of 0.08 wt. % chlorine hexahydrate with 0.14 wt. and additionally 0.05% wt. dry chlorine dioxide.

Drying is carried out under reduced pressure (0.5 kPa) at 50 ° C and after grinding a grain size of 0.12-0.20 mm is obtained.

The yield of β-D-glucan is similar to Example 2, but the chitin nitrogen content is 0.15 wt%, the ash content is 0.74 wt%.

Example 5

The procedure is similar to that of Examples 2 and 3, but to the filter cake obtained there is added a kg of acidified drinking water containing 0.011% by weight. % formic acid and 0.15 wt.

% peroxy formic acid and still 0.07 wt. peracetic acid.

The yield is similarly high, with the chitin nitrogen content further decreasing to 0.11% by weight. Dehydration is carried out by lyophilization to give a particle size of 0.04 to 0.011 mm.

Example 6

The procedure is similar to that of Example 2, but instead of sodium carbonate, a mole of the same amount of potassium carbonate is used, and instead of sodium hydroxide, only about half the amount of sodium hydroxide is used and the other half is about the same amount of potassium hydroxide.

The yield of the obtained glucan reaches 72.9%, containing 0.26% by weight. % of chitin nitrogen and 1.01 wt. ash, and is also well milled to a grain size of 0.1 to 0.3 mm.

Example 7

The procedure is similar to that of Examples 2 and 3, but an additional 0.25% by weight aqueous ammonium oxalate solution is used for defibrations and extractions. and the wet glucan is dehydrated with concentrated ethanol, the yield and purity being similar (yield 72.1% and the chitin nitrogen content is 0.31% by weight) the grain of the powder is even finer reaching 0.04 to 0.16 mm.

When lyophilization is carried out as in Example 3, the β-D-glucan particle size is 0.02 to 0.11 mm, the chitin nitrogen content is 0.24% by weight. Similar results are obtained when using a mixture of concentrated ethanol up to 48% by weight. acetone and methyl ethyl ketone.

Example 8

In a laboratory mixer, 5 kg of oyster mushroom in 10 kg of de-mineralized water is defibrated 18 h after harvesting, and then the dispersion is filtered off and the residue (cake) is poured over 10 kg of aqueous sodium carbonate solution and 0.2 wt. ammonium rope juice. Extraction is carried out at 80 + 5 ° C for 2 h. The residue from this extraction is washed with demineralized water and hydrolyzed in an aqueous solution in an amount of 10 kg containing 0.4 wt. % sodium carbonate and 0.4 wt. sodium hydroxide at 88 + 3 ° C for 3.5 h. In this way, the components and the insoluble P-1,3 / 1,6 (1,4) -glucan are substantially removed from the substrate. This was washed thoroughly with demineralized water and acidified with pure acetic acid to pH = 5. The insoluble product is blended with aqueous hydrogen peroxide at a concentration of 21% by weight with occasional stirring for 12 hours. and dehydrated with concentrated ethanol, removing ethanol under reduced pressure (0.5 kPa) at 36 + 2 ° C. 232 g of dry β-1,3 / 1,6 (1,4) -glucan powder with a chitin nitrogen content of 0.34% by weight are obtained after disintegration and micronization. and an ash of 1.13 wt% and changes 0.03 to 0.09 mm.

Example 9

The procedure is analogous to Example 8, but instead of 10 kg of a 0.4 wt.% Aqueous solution. % sodium carbonate and 0.4 wt. % sodium hydroxide also contains 0.25 wt. % of chlorine hexahydrate; % sodium peroxy formate with 0.1 wt. potassium salt of acetic acid. However, the insoluble product is whitened for at least 5 hours with an aqueous solution of sodium peroxide at a concentration of 15% by weight, then thoroughly washed with demineralized water until neutral and partially acidified with dilute acetic acid to pH = 5 and then whitened with aqueous hydrogen peroxide % at 21 wt. for 12 h and dehydrated with ethanol, the ethanol being distilled off under reduced pressure at 36 + 2 ° C.

After grinding, 233 g of dry β-1,3 / 1,6 (1,4) -glucan powder with a chitin nitrogen content of 0.71% by weight is obtained. and ash 0.91 wt.

Example 10

The procedure is similar to that of Examples 2 and 3, except that the bleaching of β-D-glucan is carried out with 200 g of hydrogen peroxide, also at a concentration of 30% by weight. instead of 50 g of chlorine dioxide hexahydrate, 70 g is applied and in addition 10 g of chlorine dioxide alone and 15 g of sodium percarbonate with 5 g of potassium percarbonate are introduced, whilst bleaching is carried out at 14 + 2 ° C, for 16 h.

The yield of β-D-glucan is 74.1%, containing 0.18% by weight. % of chitin nitrogen and 0.61 wt. ash, wherein the particle size of the β-D-glucan particles is 0.06 to 0.22 mm.

Example 11

Following the procedure of Example 2, only 150 g of 33% by weight hydrogen peroxide, 50 g of chlorine dioxide hexahydrate, 15 g of pure chlorine dioxide and 50 g of peroxy formate, 70 g of potassium peroxyformate and 100 g of sodium peroxyacetate are applied for bleaching. .

The yield of β-D-glucan reaches 72-72.9%, containing 0.11% by weight. % of chitin nitrogen and 0.61 wt. ash.

Example 12

The procedure is similar to that of Examples 2 and 3 except that the temperature and time of bleaching of crude β-D-glucan, bleaching agents, fortification defibrillation additive and the final treatment of insoluble β-D-glucan after washing with distilled water and drying, D-glucan and its purity as well as other data are shown in Table 1

Table 1

Fortifying admixture in defibrations Bleaching temperature and time Bleaching agent dehyd ratats and / post up Reagent neutral. Glucan (wt.%) Extract to glucane Content of impurities (% by weight) kind of % wt rpm rpm ° C H kind of % hmo tn. kind of % h mot n. ash N CHjCOjNa 0.21 12 25 H ₂ O ₂ 32 ClO ₂ x 6 H ₂ O 2.5 ethanol CH3CO3H 0.1 72.4 1.12 0.46 HCO ₃ K 0.15 32 6 Na ₂ O ₂ 15 HCO3K-C1O ₂ 8 + 1 liofili zácia CH3CO3H 0.2 69.1 1.01 0.44 CH3CO3K + HCO ₃ Na 0,8+ 0.7 10 24 H ₂ O ₂ + K ₂ O ₂ 5 + 18 ClO ₂ x 6 H ₂ O 10 ethanol + acet on CH ₃ CO ₂ H + CH ₃ CO ₃ H 69.9 1.11 0.38 HCOjNa 0.08 25 20 H ₂ O ₂ + Na ₂ O 2 18 + 4 ClO ₂ x 6 H ₂ O 10 ethanol CH3CO3H 0.14 73.1 0.91 0.16 - - 25 20 H ₂ O ₂ + Na ₂ O 2 18 + 4 ClO ₂ x 6 H ₂ O 10 ethanol CH3CO3H 0.14 71.9 0.94 0.16 HCO 3 H + CH ₃ CO ₃ Na 0,1+ 0.12 8 26 Na ₂ O 2 8 + 14 C1O ₂ x6 h ₂ o 10 ethanol CH3CO2H 0.18 70.5 1.01 0.35 CH3CO3K 0.14 30 20 H2O2 ^ ₂ K ₂ O + Na ₂ O ₂ 5 + 12 +6 ClO ₂ x 6 H ₂ O 4 liofili zácia CH3CO3H 0.12 74.1 0.92 0.13 CH3CO3K 0.22 34 8 K2O2 _Na2O + 2ΉΗ2Ο2 16 + 8 +5 C1O ₂ x6 H 2 O + Cl ₂ H 6 + 2 liofili zácia CH ₃ CO ₂ H 0.14 70.3 0.93 0.3

Industrial usability

The invention is useful in obtaining high purity beta-1,3 / 1,6-D-glucan as an immunomodulatory food supplement in human nutrition, but also in the fortification of feedstuffs, immunostimulants in drug formulation, to prevent not only against viral and bacterial diseases, but also against undesirable consequences of chemotherapy and radiotherapy in cancer. Last but not least, as an effective complement to the components enhancing body resistance and performance, as well as in the possibilities of efficient cultivation and use of oyster mushrooms.

Claims (7)

A process for obtaining high purity beta-1,3 / 1,6-D-glucan from oyster mushroom (Pleurotus ostreatus), preferably after washing with pure water and its depths, after washing with water, disintegration, conjugated to defibrate in an aqueous medium containing 0, 10 to 0.60 wt. at least one reagent selected from alkali metal carbonates, ammonium oxalate and a fortifying additive, wherein the pH of the aqueous suspension solution is 7.9-8.2, and after separation of the suspension, it is still extracted with an aqueous solution of sodium and / or potassium hydroxide at 0 % To 0.5 wt. and after washing, with removal of the water-soluble components, an aqueous suspension of mainly beta-1,3 / 1,6-D-glucan containing sodium hydroxide at a concentration of 0.05 to 0.2 wt. and after separating the suspension and washing, removing the water-soluble components, the suspension is mainly beta-1,3 / 1,6-Dglucan separated and after washing with a dilute aqueous carboxylic acid solution to pH 4.8-5.2 and washed with pure water, the insoluble product of the "crude" beta-1,3 / 1,6-Dglucan bleached with hydrogen peroxide and at least one other oxidant, at 6 to 36 ° C, for 3 to 26 h in an alkali metal hydroxide and / or carbonate environment, followed by dehydration organic water-soluble atoxic solvent or lyophilization, with dewatering to dryness and final treatment, characterized in that the defibrillation of the insoluble beta-1,3 / 1,6-D-glucan is carried out predominantly in an aqueous solution suspension of at least one hydroxide and / or carbonate % to alkali metal bicarbonate, additionally containing 0.03 to 0.15 wt. ammonium oxalate, and a fortifying agent formed by an alkali metal salt of peroxy formate and / or peroxyacetic acid in an amount of 0.1 to 0.45% by weight, after which it is subsequently washed with water and the water-soluble components are washed off, the agglomerated suspension, in particular beta1,3 (1,6-D-glucan) subsequently bleached at a temperature of 6 to 36 ° C for 3 to 26 hours by treatment with aqueous solutions of at least two bleaching agents selected from chlorine dioxide, chlorine dioxide hexahydrate in an amount of 1.5 to 12% by weight, hydrogen peroxide, alkali metal peroxide, peroxy formic acid and its alkali salt, peroxyacetic acid and its alkali salt, in an amount of 2 to 35% by weight, calculated on the dry weight of the suspension and the resulting beta-1,3 / 1,6-D-glucan is washed with water, acidified with acetic acid and / or peracetic acid to a content below 0.25% by weight. and finally washed with demineralized or distilled water and dried at a temperature below 65 ° C. The process for obtaining the high purity beta-1,3 / 1,6-D-glucan according to claim 1, characterized in that the fortifying admixture is an alkaline salt of peroxy formic acid and / or peroxyacetic acid in an amount of 0.05 to 0.25% by weight. Method for obtaining the high purity beta-1,3 / 1,6-D-glucan according to claims 1 and 2, characterized in that the bleaching of the beta-1,3 / 1,6-D-glucan suspension is carried out with chlorine dioxide, preferably in the form of hexahydrate, in an amount of 2 to 8% by weight, calculated on the dry weight of the suspension. Method for obtaining the high purity beta-1,3 / 1,6-D-glucan according to claims 1 to 3, characterized in that the co-bleaching of the beta-1,3 / 1,6-D-glucan suspension is carried out with hydrogen peroxide and / or or an alkaline salt thereof, selected from sodium peroxide and potassium peroxide, in an amount of 2 to 25% by weight, calculated on the dry weight of the suspension. A process for obtaining high purity beta-1,3 / 1,6-D-glucan according to claims 1 to 4, characterized in that the co-bleaching of the beta-1,3 / 1,6-D-glucan suspension is carried out with at least one acid selected from peroxy formic acid and peroxyacetic acid and / or their alkali salts in an amount of 3 to 15% by weight, calculated on the weight of the suspension. Process for obtaining high purity beta-1,3 / 1,6-D-glucan according to claims 1 to 5, characterized in that the pure acidified water is acetic acid and / or peracetic acid with a content of 0.25% by weight each. it consists of drinking or distilled water. A process for obtaining the high purity beta-1,3 / 1,6-D-glucan according to claims 1 to 6, characterized in that the dried beta-1,3 / 1,6-D-glucan is adapted to an applicable form, such as by reprocessing. , micronization, gelling and formulation with other substances to produce nutritional and therapeutic, especially immunostimulatory effect.