TH13577C3 - A primer and DNA probe set for detecting dengue virus by Polymerase Chain Reaction or PCR. Color-coded measuring sheet (dipstick) - Google Patents

Abstract

Revised invention summary 24/08/2016 Primer and DNA probe kit for detecting dengue virus with Polymerase Chain Reaction. ) Or PCR (PCR), combined with a color strip (dipstick), shows the invention as follows: 1. It is a simple, convenient and fast test kit. Results can be obtained in 1 hour, 30 minutes to 2 hours. Used to inspect in the field (point-of-care) 2. High specificity 3. High accuracy can be detected at the lowest quantity (limit of detdction) at the DNA content. Level about nanograms (nanogram, 10-9) to attogram (attogram, 10-18) 4. Used for detecting dengue virus (dengue virus) ------------- -------------------------------------------- Primer set ( primer) and detector (DNA probe) for detecting dengue virus by polymerase chain reaction or PCR (PCR) in conjunction with a color stripe probe. (dipstick) illustrates the brief invention as follows: 1. It is a simple, convenient and fast test set. Results in about 1 hour 30 minutes can be used for field examination (point-of-care) 2. High specificity 3. High accuracy can be detected at the lowest quantity (limit of detdction) at the DNA content. Levels about nanograms (nanogram, 10-9) to attogram (attogram, 10-18) 4. Used for detecting dengue virus (dengue virus).

Claims (6)

Disclaimer (all) which will not appear on the announcement page: Amendment 30 Jun 2017 1. Primer and DNA probe kit for detecting Denki virus ( dengue virus) with a polymerase chain reaction (PCR), combined with a dipstick, consists of two primers specific to viral sequences. Dengue virus to 2 locations and a detector (DNA probe) for detecting dengue virus as follows: primer dengue _F (Deng_F) sequence (5 \ '- 3 \ ') gAMACMgCHTgggAYTTTgg Primer Deng _ R (Deng_R) Base Sequence (5 \' - 3 \ ') gCYTTKCCCCAKSTYTTCCAD PTA Detector_F3 Deng (Probe-PTA_F3Deng) Sequence (5 \ '- 3 \') GTBCACACHTGGACAGARC 2. Primer and DNA probe set for detecting dengue virus with enough chain reaction. Polymerase Chain Reaction or PCR (PCR) is combined with a dipstick in accordance with claim 1 where the optimum conditions for product testing from PCR reactions ) There is a unique The PCR reaction was performed at 3 temperature ranges of 90-95 degrees Celsius, 45-50 degrees Celsius and 70-75 degrees Celsius for 90-120 minutes and examined the target DNA yield by using sheet techniques. Dipstick test. 3. Primer and DNA probe for detecting dengue virus by Polymerase Chain Reaction. ) Or PCR, according to claim 1, where dengue virus detectors have a DNA probe design method for detecting dengue virus. (dengue virus) on a color stripe (dipatick) test sheet has the characteristics of The detector is stuck FITC (FICT) label at 5 \ 'end for the determination of the yield of PCR reaction (PCR) on a dipstick (dipstick) measuring plate has the following sequences: FITC-5 \ '- GTBCACACHTGGACAGARC-3 \' 4. A primer and DNA probe for detecting dengue virus (PCR) in the joints. The first right is composed of main components for use in the reaction: forward primer, Deng_F and reverse primer Deng_R. Each 10-100 micromolar ((formula) M), mixed with deoxynucleotide triphosphate. (Deoxynucleotide triphosphate, dNTPs) 10-100 mmol (mM), Magnesium Chloride (MgCl2) 2-6 Mm, Enzyme Tack DNA Polymerase ( Tag DNA polymerase) 0.1-1 units (unit, U) and buffer for 1-10 times supplied buffer (X) 5. Primer and DNA probe set for detecting virus infection. Dengue virus by PCR technique in claim 1 is unique: The product of the PCR reaction (PCR product) can be detected with a color strip (dipstick). 6. A primer and a detector (DNA probe) for detecting dengue virus. ) With PCR technique in claim 1 for dengue virus diagnosis. Revised 24/08/2016 1. Primer and detectors (DNA probe) for detecting dengue virus by Polymerase Chain Reaction or PCR (PCR), in conjunction with a dipstick, consisting of a primer Two specific sequences of dengue virus to 2 locations and a detector (DNA probe) for detecting dengue virus as follows: primer dengue_F ( Deng_F) Base Sequence (5 \ '- 3 \') gAMACMgCHTgggAYTTTgg Primer Deng_F (Deng_R) Base Sequence (5 \ '- 3 \') gCYTTKCCCCAKSTYTTCCAD PTA Detector _F 3 Deng (Probe-PTA_F3Deng) Sequence (5 \ '- 3 \') GTBCACACHTGGACAGARC 2. Primer and DNA probe set for detecting dengue virus with enough chain reaction. Polymerase Chain Reaction or PCR (PCR) is combined with a dipstick in accordance with claim 1 where the optimum conditions for product testing from PCR reactions ) There is a unique The PCR reaction was performed at 3 temperature ranges of 90-95 degrees Celsius, 45-50 degrees Celsius and 70-75 degrees Celsius for 90-120 minutes and examined the target DNA yield by using sheet techniques. Dipstick test. 3. Primer and DNA probe for detecting dengue virus by Polymerase Chain Reaction. ) Or PCR, according to claim 1, where dengue virus detectors have a DNA probe design method for detecting dengue virus. (dengue virus) on a color stripe (dipatick) test sheet has the characteristics of The detector is stuck FITC (FICT) label at 5 \ 'end for the determination of the yield of PCR reaction (PCR) on a dipstick (dipstick) measuring plate has the following sequences: FITC-5 \ '- GTBCACACHTGGACAGARC-3 \' 4. A primer and DNA probe for detecting dengue virus (PCR) in the joints. Hold the first right consisting of main components for use in the reaction: forward primer Deng_F and reverse priner Deng_R. Each 10-100 micromolar ((formula) M), mixed with deoxynucleotide triphosphate. (Deoxynucleotide triphosphate, dNTPs) 10-100 mmol (mM), Magnesium Chloride (MgCl2) 2-6 Mm, Enzyme Tack DNA Polymerase ( Tag DNA polymerase) 0.1-1 units (unit, U) and buffer for 1-10 times supplied buffer (X) 5. primer and DNA probe for detecting deng virus. Ki (dengue virus) by PCR technique in claim 1 is unique. The product from PCR reaction (PCR product) can be examined with a dipstick, 6 primer and DNA probe for detecting dengue virus. Using the PCR technique for the diagnosis of dengue virus in human blood samples ------------------------- ----------------------------------------- 1.Primer and DNA probe kit for detecting dengue virus by Polymerase Chain Reaction or PCR. In addition to the dipstick, it consists of two primers that are specific to dengue virus sequences to 2 locations and a detector (DNA probe) for detecting dengue virus. Ki (dengue virus) follows primer deng _F (Deng_F) sequence (5 \ '- 3 \') gAMACMgCHTgggAYTTTgg primer deng_F (Deng_R) sequence (5 \ '- 3 \ ') gCYTTKCCCCAKSTYTTCCAD PTA_F3 detectors. (Probe-PTA_F3Deng) Base sequence (5 \ '- 3 \') GTBCACACHTGGACAGARC 2. A primer and DNA probe for detecting dengue virus by polymerase chain reaction or PCR. In addition to the dipstick test sheet according to claim 1, the optimum conditions for testing yield from PCR reactions (PCR) are characterized as The PCR reaction was performed at 3 temperature ranges of 90-95 degrees Celsius, 45-50 degrees Celsius and 70-75 degrees Celsius for 90-120 minutes and examined the target DNA yield by using sheet techniques. Measure the color stripe (dipstick) 3.Primer and DNA probe set for detecting dengue virus by Polymerase Chain Reaction or PCR. According to claim 1, the dengue virus detectors have a DNA probe design method for detecting dengue virus on the test strips. The dipatick is unique. The detector is stuck FITC (FICT) label at 5 \ 'end for the determination of the yield of PCR reaction (PCR) on a dipstick (dipstick) measuring plate has the following sequences: FITC-5 \ '- GTBCACACHTGGACAGARC-3 \' 4. The primer and DNA probe for the detection of dengue virus by PCR technique in claim 1 contains the main components for use in the reaction. Here are forward primer Deng_F (Deng_F) and reverse priner Deng_F (Deng_R) each 10-100 micromolar (( Formula) M), mixed with deoxynucleotide triphosphate (Deoxynucleotide triphosphate, dNTPs) 10-100 mmol (mM), Magnesium Chloride (MgCl2) 2-6 Mm, Enzyme Tack DNA Polymerase ( Tag DNA polymerase) 0.1-1 units (unit, U) and buffer for Enzyme TAC DNA polymerase (supplied buffer) 1-10 times (X). 5. The primer and DNA probe for detecting dengue virus by PCR technique in claim 1 are unique. The product from PCR reaction (PCR product) can be checked with a colored dipstick. 6. A primer and a DNA probe for detecting dengue virus with PCR technique for the diagnosis of dengue virus in dengue virus. Human blood sample