TR199802503A2 - Endojen gen aktivasyonu için hücrelerin optimizasyonu. - Google Patents
Endojen gen aktivasyonu için hücrelerin optimizasyonu.Info
- Publication number
- TR199802503A2 TR199802503A2 TR1998/02503A TR9802503A TR199802503A2 TR 199802503 A2 TR199802503 A2 TR 199802503A2 TR 1998/02503 A TR1998/02503 A TR 1998/02503A TR 9802503 A TR9802503 A TR 9802503A TR 199802503 A2 TR199802503 A2 TR 199802503A2
- Authority
- TR
- Turkey
- Prior art keywords
- cells
- relates
- expression
- optimization
- endogenous gene
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 230000004913 activation Effects 0.000 title 1
- 238000005457 optimization Methods 0.000 title 1
- 230000014509 gene expression Effects 0.000 abstract 5
- 108091006088 activator proteins Proteins 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 2
- 230000006801 homologous recombination Effects 0.000 abstract 2
- 238000002744 homologous recombination Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108010052160 Site-specific recombinase Proteins 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Bulus, hücrelerde gen sentezlemesinin optimize edilmesi için bir isleme iliskindir. Bulusun bir birinci yani, homolog rekombinasyon vasitasiyla hücrenin genomu içine bir heterolog sentezleme kontrol dizisinin verilmesinin yanisira girilen yabanci DNA' nin mevkiye-özgü rekombinaz-aracili kesilmesi ve daha baska heterolog sentezleme kontrol dizileri ve/ veya büyütme genleri ile degistirilmesiyle, bir ökariyotik hücrede endojen biçimde mevcut olan bir nükleik asit dizisinin sentezlenmesinin degistirilmesi için bir isleme iliskindir. Ilave olarak, bulus, bir aktivatör proteini ya da bir aktivatör protein kompleksinin baglandigi bir ya da bir kaç nükleik asit dizisinin, bir hedef genin sentezlenmesinin degistirilmesi amaciyla homolog rekombinasyon ile bir ökariyotik hücrenin genomu içine katilmasina dairdir.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97121075 | 1997-12-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TR199802503A3 TR199802503A3 (tr) | 1999-06-21 |
| TR199802503A2 true TR199802503A2 (tr) | 1999-06-21 |
Family
ID=8227717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR1998/02503A TR199802503A2 (tr) | 1997-12-01 | 1998-12-01 | Endojen gen aktivasyonu için hücrelerin optimizasyonu. |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US7008764B1 (tr) |
| EP (3) | EP1464705B1 (tr) |
| JP (1) | JP4629813B2 (tr) |
| KR (1) | KR100367062B1 (tr) |
| CN (2) | CN1232644C (tr) |
| AR (4) | AR018022A1 (tr) |
| AT (3) | ATE360067T1 (tr) |
| AU (1) | AU757930B2 (tr) |
| BR (1) | BR9805682A (tr) |
| CA (1) | CA2252970C (tr) |
| DE (3) | DE59813438D1 (tr) |
| DK (3) | DK0957165T3 (tr) |
| ES (3) | ES2284228T3 (tr) |
| PT (3) | PT919619E (tr) |
| TR (1) | TR199802503A2 (tr) |
| ZA (1) | ZA9810915B (tr) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6316253B1 (en) | 1999-11-01 | 2001-11-13 | Chiron Corporation | Expression vectors, transfection systems, and method of use thereof |
| CA2401677A1 (en) * | 2000-03-03 | 2001-09-13 | University Of Utah Research Foundation | Gene targeting method |
| DE10023887A1 (de) * | 2000-05-17 | 2001-11-29 | Axel Haverich | Verfahren zur transienten Insertion genetischer Elemente |
| WO2003012036A2 (en) | 2001-07-27 | 2003-02-13 | The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| WO2003087341A2 (en) | 2002-01-23 | 2003-10-23 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
| EP2806025B1 (en) | 2002-09-05 | 2019-04-03 | California Institute of Technology | Use of zinc finger nucleases to stimulate gene targeting |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| US20070134796A1 (en) * | 2005-07-26 | 2007-06-14 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
| US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US20120196370A1 (en) | 2010-12-03 | 2012-08-02 | Fyodor Urnov | Methods and compositions for targeted genomic deletion |
| US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| AU2005287278B2 (en) * | 2004-09-16 | 2011-08-04 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
| CN103484496A (zh) * | 2013-07-31 | 2014-01-01 | 新乡医学院 | 一种报告基因重组在染色体目的基因上的方法、位点及用途 |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| CA2971589C (en) | 2014-12-18 | 2021-09-28 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| WO2017201081A1 (en) | 2016-05-16 | 2017-11-23 | Agilome, Inc. | Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US568772A (en) * | 1896-10-06 | Albert s | ||
| CA1293460C (en) * | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| GB2187415A (en) | 1986-03-05 | 1987-09-09 | Deere & Co | Machine for forming cylindrical bales of crop |
| JPS62265992A (ja) * | 1986-05-12 | 1987-11-18 | Ajinomoto Co Inc | ヒト細胞を用いる有用物質の製造方法 |
| US4956288A (en) * | 1988-04-22 | 1990-09-11 | Biogen, Inc. | Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA |
| FR2646438B1 (fr) | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
| EP0945515A3 (en) * | 1989-11-06 | 2002-08-21 | Cell Genesys, Inc. | Production of proteins using homologous recombination |
| ES2151463T5 (es) * | 1989-12-22 | 2012-10-29 | Merck Serono Sa | Constructos de ADN para la activación y la modificación de la expresión de genes endógenos |
| US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| WO1992015694A1 (en) * | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
| US6270989B1 (en) * | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
| WO1993015191A1 (en) * | 1992-01-24 | 1993-08-05 | Life Technologies, Inc. | Modulation of enzyme activities in the in vivo cloning of dna |
| TW402639B (en) * | 1992-12-03 | 2000-08-21 | Transkaryotic Therapies Inc | Protein production and protein delivery |
| US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
| ATE244311T1 (de) * | 1993-12-23 | 2003-07-15 | Merck & Co Inc | Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen |
| US6130364A (en) * | 1995-03-29 | 2000-10-10 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
| US5882914A (en) * | 1995-06-06 | 1999-03-16 | The Johns Hopkins University School Of Medicine | Nucleic acids encoding the hypoxia inducible factor-1 |
| US5695977A (en) * | 1995-08-31 | 1997-12-09 | Genetic Information Research Institute | Site directed recombination |
| AUPN903196A0 (en) * | 1996-03-29 | 1996-04-26 | Australian National University, The | Single-step excision means |
| US6020144A (en) * | 1996-09-12 | 2000-02-01 | Symbiontics, Inc. | Sustained delivery device comprising a Leishmania protozoa and methods of making and using the same |
-
1998
- 1998-11-19 AU AU93284/98A patent/AU757930B2/en not_active Expired
- 1998-11-30 ZA ZA9810915A patent/ZA9810915B/xx unknown
- 1998-11-30 CA CA2252970A patent/CA2252970C/en not_active Expired - Lifetime
- 1998-11-30 KR KR10-1998-0051930A patent/KR100367062B1/ko not_active Expired - Lifetime
- 1998-12-01 BR BR9805682-4A patent/BR9805682A/pt not_active Application Discontinuation
- 1998-12-01 US US09/203,500 patent/US7008764B1/en not_active Expired - Lifetime
- 1998-12-01 DK DK99112607T patent/DK0957165T3/da active
- 1998-12-01 PT PT98122807T patent/PT919619E/pt unknown
- 1998-12-01 DE DE59813438T patent/DE59813438D1/de not_active Expired - Lifetime
- 1998-12-01 CN CNB981271782A patent/CN1232644C/zh not_active Expired - Lifetime
- 1998-12-01 ES ES99112607T patent/ES2284228T3/es not_active Expired - Lifetime
- 1998-12-01 EP EP04006966A patent/EP1464705B1/de not_active Expired - Lifetime
- 1998-12-01 PT PT99112607T patent/PT957165E/pt unknown
- 1998-12-01 TR TR1998/02503A patent/TR199802503A2/tr unknown
- 1998-12-01 DE DE59813976T patent/DE59813976D1/de not_active Revoked
- 1998-12-01 ES ES98122807T patent/ES2258809T5/es not_active Expired - Lifetime
- 1998-12-01 DE DE59813413T patent/DE59813413D1/de not_active Expired - Lifetime
- 1998-12-01 ES ES04006966T patent/ES2259422T3/es not_active Expired - Lifetime
- 1998-12-01 AT AT99112607T patent/ATE360067T1/de active
- 1998-12-01 AR ARP980106075A patent/AR018022A1/es active IP Right Grant
- 1998-12-01 JP JP34223498A patent/JP4629813B2/ja not_active Expired - Fee Related
- 1998-12-01 PT PT04006966T patent/PT1464705E/pt unknown
- 1998-12-01 EP EP98122807.5A patent/EP0919619B2/de not_active Expired - Lifetime
- 1998-12-01 AT AT04006966T patent/ATE320491T1/de active
- 1998-12-01 EP EP99112607A patent/EP0957165B1/de not_active Revoked
- 1998-12-01 DK DK98122807.5T patent/DK0919619T4/da active
- 1998-12-01 DK DK04006966T patent/DK1464705T3/da active
- 1998-12-01 CN CNB2004100638729A patent/CN100519751C/zh not_active Expired - Lifetime
- 1998-12-01 AT AT98122807T patent/ATE318904T1/de active
-
1999
- 1999-10-08 AR ARP990105097A patent/AR020893A2/es active IP Right Grant
- 1999-10-08 AR ARP990105095A patent/AR020750A2/es active IP Right Grant
- 1999-10-08 AR ARP990105096A patent/AR020892A2/es active IP Right Grant
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