TR200401075T4 - Hedef türlere bağlanan bileşikleri belirlemek için flüoresan korelasyon spektroskopisinin kullanımı - Google Patents

Hedef türlere bağlanan bileşikleri belirlemek için flüoresan korelasyon spektroskopisinin kullanımı

Info

Publication number
TR200401075T4
TR200401075T4 TR2004/01075T TR200401075T TR200401075T4 TR 200401075 T4 TR200401075 T4 TR 200401075T4 TR 2004/01075 T TR2004/01075 T TR 2004/01075T TR 200401075 T TR200401075 T TR 200401075T TR 200401075 T4 TR200401075 T4 TR 200401075T4
Authority
TR
Turkey
Prior art keywords
target species
test compound
correlation spectroscopy
fluorescence correlation
bind
Prior art date
Application number
TR2004/01075T
Other languages
English (en)
Inventor
E. Epps Dennis
K. Tomich Paul
J. Kezdy Ferenc
K. Marschke Charles
Original Assignee
Pharmacia & Upjohn Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia & Upjohn Company filed Critical Pharmacia & Upjohn Company
Publication of TR200401075T4 publication Critical patent/TR200401075T4/tr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/12—Condition responsive control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Mevcut bulus bir hedef üre baglanan bir test bilesiginin belirlenmesi için bir yöntem saglamaktadir. Yöntem asagidaki kademeleri içerir; en az bir test bilesiginin izotermal denatürasyon kosullari altinda kuluçkalanmasi, her bir test bilesigi ve en az bir hedef tür içerir burada izotermal denatürasyona ugramasina neden olacak kadar etkilidir; her bir hedef türünün denatürasyon sinyalinin en az bir test bilesigi varliginda hedef molekülün yayilma özelliklerindeki bir degisikligin flüoresan korelasyon spektroskopisinin kullanimi ile tespit edilmesi; ve her bir hedef türünün en az bir test bilesigi varliginda denatürasyon sinyalinin ayni hedef türünün en az bir test bilesigi yoklugunda ayni izotermal denatürasyon kosullari altindaki denatürasyon sinyalinin karsilastirilmasi.
TR2004/01075T 1999-12-09 2000-12-07 Hedef türlere bağlanan bileşikleri belirlemek için flüoresan korelasyon spektroskopisinin kullanımı TR200401075T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/458,172 US6582907B1 (en) 1999-12-09 1999-12-09 Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions

Publications (1)

Publication Number Publication Date
TR200401075T4 true TR200401075T4 (tr) 2004-07-21

Family

ID=23819671

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2004/01075T TR200401075T4 (tr) 1999-12-09 2000-12-07 Hedef türlere bağlanan bileşikleri belirlemek için flüoresan korelasyon spektroskopisinin kullanımı

Country Status (13)

Country Link
US (2) US6582907B1 (tr)
EP (1) EP1236047B1 (tr)
JP (1) JP2003517609A (tr)
AT (1) ATE265684T1 (tr)
AU (1) AU777163B2 (tr)
CA (1) CA2392239A1 (tr)
DE (1) DE60010323T2 (tr)
DK (1) DK1236047T3 (tr)
ES (1) ES2218280T3 (tr)
HK (1) HK1047158B (tr)
PT (1) PT1236047E (tr)
TR (1) TR200401075T4 (tr)
WO (1) WO2001044817A2 (tr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
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GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6517781B1 (en) * 1997-06-02 2003-02-11 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
DE10013854A1 (de) * 2000-03-17 2001-09-20 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
AU2003244122A1 (en) * 2002-06-21 2004-01-06 Olympus Corporation Biomolecule analyzer
JP2005534945A (ja) * 2002-08-01 2005-11-17 センサー テクノロジーズ リミティド ライアビリティー カンパニー 分子間相互作用の計測方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2006013109A1 (en) * 2004-08-06 2006-02-09 Epfl Swiss Federal Institute Of Technology-Lausanne Method for testing a substance interacting with a target molecule
EP1848983B1 (en) * 2005-02-15 2013-03-27 Tata Institute of Fundamental Research Fluorescence correlation microscopy with real-time alignment readout
DE102007045666B4 (de) * 2007-09-25 2026-04-02 Carl Zeiss Microscopy Gmbh Verfahren und Anordnung zur Ausgabe von Restfehlern einer an eine Punktemenge angepassten Funktion
KR100963305B1 (ko) 2008-01-23 2010-06-11 건국대학교 산학협력단 단백질 내의 도메인 간 변성과정 분석방법
US12400741B2 (en) * 2020-02-28 2025-08-26 Howard University Selective extraction of late exponentials

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5585277A (en) 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
CA2236339A1 (en) 1995-12-07 1997-06-12 Robert T. Sauer A fluorescence-based screening method for identifying ligands
EP0914608A1 (en) 1996-05-09 1999-05-12 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
EP0836090A1 (en) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Method of analysis of samples by determination of the distribution of specific brightnesses of particles
DK1036192T3 (da) 1997-12-05 2003-06-10 Upjohn Co Fluorescensbaserede screeningsanalyser med høj hastighedforproteinkinaser og phosphataser
US6242190B1 (en) 1999-12-01 2001-06-05 John Hopkins University Method for high throughput thermodynamic screening of ligands
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions

Also Published As

Publication number Publication date
ATE265684T1 (de) 2004-05-15
HK1047158B (en) 2004-12-03
PT1236047E (pt) 2004-08-31
CA2392239A1 (en) 2001-06-21
HK1047158A1 (en) 2003-02-07
DK1236047T3 (da) 2004-07-05
US6582907B1 (en) 2003-06-24
US20030219820A1 (en) 2003-11-27
DE60010323T2 (de) 2005-05-25
EP1236047A2 (en) 2002-09-04
EP1236047B1 (en) 2004-04-28
AU777163B2 (en) 2004-10-07
DE60010323D1 (de) 2004-06-03
AU4519801A (en) 2001-06-25
WO2001044817A2 (en) 2001-06-21
ES2218280T3 (es) 2004-11-16
JP2003517609A (ja) 2003-05-27
WO2001044817A3 (en) 2002-05-10

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