ATE265684T1 - Fluoreszenzkorrelationsspektroskopie zur identifizierung von verbindung-ziel-bindung - Google Patents

Fluoreszenzkorrelationsspektroskopie zur identifizierung von verbindung-ziel-bindung

Info

Publication number
ATE265684T1
ATE265684T1 AT00992663T AT00992663T ATE265684T1 AT E265684 T1 ATE265684 T1 AT E265684T1 AT 00992663 T AT00992663 T AT 00992663T AT 00992663 T AT00992663 T AT 00992663T AT E265684 T1 ATE265684 T1 AT E265684T1
Authority
AT
Austria
Prior art keywords
target species
test compound
correlation spectroscopy
fluorescence correlation
denaturing conditions
Prior art date
Application number
AT00992663T
Other languages
English (en)
Inventor
Dennis E Epps
Paul K Tomich
Ferenc J Kezdy
Charles K Marschke
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Application granted granted Critical
Publication of ATE265684T1 publication Critical patent/ATE265684T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/12—Condition responsive control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT00992663T 1999-12-09 2000-12-07 Fluoreszenzkorrelationsspektroskopie zur identifizierung von verbindung-ziel-bindung ATE265684T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/458,172 US6582907B1 (en) 1999-12-09 1999-12-09 Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
PCT/US2000/042642 WO2001044817A2 (en) 1999-12-09 2000-12-07 Fluorescence correlation spectroscopy to identify compound-target binding

Publications (1)

Publication Number Publication Date
ATE265684T1 true ATE265684T1 (de) 2004-05-15

Family

ID=23819671

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00992663T ATE265684T1 (de) 1999-12-09 2000-12-07 Fluoreszenzkorrelationsspektroskopie zur identifizierung von verbindung-ziel-bindung

Country Status (13)

Country Link
US (2) US6582907B1 (de)
EP (1) EP1236047B1 (de)
JP (1) JP2003517609A (de)
AT (1) ATE265684T1 (de)
AU (1) AU777163B2 (de)
CA (1) CA2392239A1 (de)
DE (1) DE60010323T2 (de)
DK (1) DK1236047T3 (de)
ES (1) ES2218280T3 (de)
HK (1) HK1047158B (de)
PT (1) PT1236047E (de)
TR (1) TR200401075T4 (de)
WO (1) WO2001044817A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6517781B1 (en) * 1997-06-02 2003-02-11 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
DE10013854A1 (de) * 2000-03-17 2001-09-20 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
AU2003244122A1 (en) 2002-06-21 2004-01-06 Olympus Corporation Biomolecule analyzer
WO2004013610A2 (en) * 2002-08-01 2004-02-12 Sensor Technologies Llc Method of measuring molecular interactions
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2006013109A1 (en) * 2004-08-06 2006-02-09 Epfl Swiss Federal Institute Of Technology-Lausanne Method for testing a substance interacting with a target molecule
WO2006087727A1 (en) * 2005-02-15 2006-08-24 Tata Institute Of Fundamental Research Fluorescence correlation microscopy with real-time alignment readout
DE102007045666B4 (de) * 2007-09-25 2026-04-02 Carl Zeiss Microscopy Gmbh Verfahren und Anordnung zur Ausgabe von Restfehlern einer an eine Punktemenge angepassten Funktion
KR100963305B1 (ko) 2008-01-23 2010-06-11 건국대학교 산학협력단 단백질 내의 도메인 간 변성과정 분석방법
US12400741B2 (en) * 2020-02-28 2025-08-26 Howard University Selective extraction of late exponentials

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585277A (en) 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
WO1997020952A1 (en) 1995-12-07 1997-06-12 Scriptgen Pharmaceuticals, Inc. A fluorescence-based screening method for identifying ligands
US6036920A (en) 1996-05-09 2000-03-14 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
EP0836090A1 (de) 1996-10-12 1998-04-15 Evotec BioSystems GmbH Probenanalyseverfahren durch Bestimmung der spezifischen Helligkeitsverteilung von Teilchen
PT1036192E (pt) 1997-12-05 2003-06-30 Upjohn Co Ensaios de pesquisa de alto rendimento a base de fluorescencia para proteina-quinases e -fosfatases
US6242190B1 (en) 1999-12-01 2001-06-05 John Hopkins University Method for high throughput thermodynamic screening of ligands
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions

Also Published As

Publication number Publication date
DE60010323T2 (de) 2005-05-25
AU4519801A (en) 2001-06-25
AU777163B2 (en) 2004-10-07
EP1236047B1 (de) 2004-04-28
US6582907B1 (en) 2003-06-24
US20030219820A1 (en) 2003-11-27
HK1047158B (en) 2004-12-03
JP2003517609A (ja) 2003-05-27
HK1047158A1 (en) 2003-02-07
EP1236047A2 (de) 2002-09-04
TR200401075T4 (tr) 2004-07-21
ES2218280T3 (es) 2004-11-16
CA2392239A1 (en) 2001-06-21
PT1236047E (pt) 2004-08-31
WO2001044817A2 (en) 2001-06-21
DE60010323D1 (de) 2004-06-03
DK1236047T3 (da) 2004-07-05
WO2001044817A3 (en) 2002-05-10

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