TR200905663A2 - Enzimatik olmayan yöntemle kandan dna izolasyonu. - Google Patents
Enzimatik olmayan yöntemle kandan dna izolasyonu.Info
- Publication number
- TR200905663A2 TR200905663A2 TR2009/05663A TR200905663A TR200905663A2 TR 200905663 A2 TR200905663 A2 TR 200905663A2 TR 2009/05663 A TR2009/05663 A TR 2009/05663A TR 200905663 A TR200905663 A TR 200905663A TR 200905663 A2 TR200905663 A2 TR 200905663A2
- Authority
- TR
- Turkey
- Prior art keywords
- blood
- spin column
- dna isolation
- dna
- enzymatic method
- Prior art date
Links
- 239000008280 blood Substances 0.000 title abstract 3
- 210000004369 blood Anatomy 0.000 title abstract 3
- 238000007399 DNA isolation Methods 0.000 title abstract 2
- 238000006911 enzymatic reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- -1 amino modified silica particles Chemical class 0.000 abstract 1
- 238000009739 binding Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000000945 filler Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Buluş, kandan genomik DNA'nın pzr'nu engelleyecek safsızlıklardan arındırılarak izole edilmesi için bir yöntemdir. Kandan DNA izolasyonu, parçalama-bağlama, yıkama ve elüsyon çözletileri kullanılarak ve ucuna amino modifiye silika partiküller ile hazırlanmış filtre yerleştirilmiş, spin kolonda gerçekleştirilmektedir. Buluşta kullanılan yöntem, mevcut yöntemlerdeki gibi bir enzimatik işlem içermemektedir, böylece buluş sayesinde protein parçalayıcı bir enzime ve ısıtma işlemine gereksinim ortadan kaldınlmıştır. Buluşun içeriğinde tanımlanan çözeltiler ve kolon dolgu materyali ile oluşturulabilecek spin kolon ile oluşturulacak tam kandan spin kolon DNA saflaştırma kitiile buluş sananyiye uygulanabilir.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2009/05663A TR200905663A2 (tr) | 2009-07-22 | 2009-07-22 | Enzimatik olmayan yöntemle kandan dna izolasyonu. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2009/05663A TR200905663A2 (tr) | 2009-07-22 | 2009-07-22 | Enzimatik olmayan yöntemle kandan dna izolasyonu. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TR200905663A2 true TR200905663A2 (tr) | 2011-02-21 |
Family
ID=44948432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR2009/05663A TR200905663A2 (tr) | 2009-07-22 | 2009-07-22 | Enzimatik olmayan yöntemle kandan dna izolasyonu. |
Country Status (1)
| Country | Link |
|---|---|
| TR (1) | TR200905663A2 (tr) |
-
2009
- 2009-07-22 TR TR2009/05663A patent/TR200905663A2/tr unknown
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