TR201907065T4 - Aptamer kullanarak hedef maddeyi tespit yöntemi. - Google Patents

Aptamer kullanarak hedef maddeyi tespit yöntemi. Download PDF

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Publication number
TR201907065T4
TR201907065T4 TR2019/07065T TR201907065T TR201907065T4 TR 201907065 T4 TR201907065 T4 TR 201907065T4 TR 2019/07065 T TR2019/07065 T TR 2019/07065T TR 201907065 T TR201907065 T TR 201907065T TR 201907065 T4 TR201907065 T4 TR 201907065T4
Authority
TR
Turkey
Prior art keywords
aptamer
detecting
target substance
target material
kit
Prior art date
Application number
TR2019/07065T
Other languages
English (en)
Inventor
Youn Kim So
Original Assignee
Univ Dongguk Ind Acad Coop
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020090012288A external-priority patent/KR101437616B1/ko
Application filed by Univ Dongguk Ind Acad Coop filed Critical Univ Dongguk Ind Acad Coop
Publication of TR201907065T4 publication Critical patent/TR201907065T4/tr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Mevcut buluş, bir aptamer kullanarak bir hedef materyali tespit etmek için bir metot ve kite ve daha özel olarak, birinci aptamer, hedef malzeme ve ikinci aptamer arasında sandviçlenmiş bir bağ oluşturmak üzere katı bir fazda immobilize edilmiş bir birinci aptamere, bir numune ve ikinci aptamerin eklendiği bir hedef materyali tespit etmek için bir metot ve kit ile ilgilidir.
TR2019/07065T 2009-02-16 2010-02-16 Aptamer kullanarak hedef maddeyi tespit yöntemi. TR201907065T4 (tr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090012288A KR101437616B1 (ko) 2009-02-16 2009-02-16 압타머를 이용한 ｆｅｔ 센서 기반 타겟물질의 검출방법
KR20090012287 2009-02-16

Publications (1)

Publication Number Publication Date
TR201907065T4 true TR201907065T4 (tr) 2019-06-21

Family

ID=42562220

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2019/07065T TR201907065T4 (tr) 2009-02-16 2010-02-16 Aptamer kullanarak hedef maddeyi tespit yöntemi.

Country Status (6)

Country Link
US (1) US9075053B2 (tr)
EP (3) EP3495501B1 (tr)
CN (1) CN102395684B (tr)
ES (1) ES2730808T3 (tr)
TR (1) TR201907065T4 (tr)
WO (1) WO2010093223A2 (tr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101892540B1 (ko) * 2012-05-10 2018-08-28 삼성전자주식회사 바이오 물질의 알에프 특성 측정 방법 및 장치
US9310357B2 (en) 2012-09-28 2016-04-12 Src, Inc. Detection of chemical and biological agents using oligonucleotide aptamers
CN102980888A (zh) * 2012-12-18 2013-03-20 合肥工业大学 基于核酸适配体探针的一步法非标记型双酚a的快速比色检测法
DE102013211125A1 (de) * 2013-06-14 2014-12-18 Siemens Aktiengesellschaft Verfahren zur kombinierten Quantifizierung und Sequenzierung von mindestens einer Ziel-Nukleinsäure
CN111443069A (zh) 2013-10-28 2020-07-24 多茨技术公司 过敏原检测
EP3289064B1 (en) 2015-04-29 2020-11-18 DOTS Technology Corp. Compositions and methods for allergen detection
AU2019352500B2 (en) 2018-10-02 2024-12-12 WearOptimo Pty Ltd Treatment delivery system
WO2020069564A1 (en) 2018-10-02 2020-04-09 WearOptimo Pty Ltd A system for determining fluid level in a biological subject
WO2020069569A1 (en) 2018-10-02 2020-04-09 WearOptimo Pty Ltd Actuator system
KR102806191B1 (ko) 2018-12-21 2025-05-09 일루미나, 인코포레이티드 감지 시스템들
AU2020359292A1 (en) * 2019-10-01 2022-04-21 WearOptimo Pty Ltd Analyte measurement system
US11488326B2 (en) 2019-12-11 2022-11-01 Ananya Achanta Estimation of bisphenol a using image analysis
CN112098487A (zh) * 2020-08-08 2020-12-18 青岛科技大学 一种纳米孔道光电化学dna传感器及其制备方法与应用
CN113960128B (zh) * 2020-12-15 2022-08-23 有研工程技术研究院有限公司 基于钾离子适配体修饰的硅纳米线场效应管生物传感器
WO2025207992A1 (en) * 2024-03-28 2025-10-02 Worcester Polytechnic Institute Aptamer-based pollutant protection

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
CA2433674A1 (en) * 2001-01-05 2002-07-11 Invitrogen Corporation Method for relative quantification of attached nucleic acids
US20030064530A1 (en) * 2001-07-04 2003-04-03 Nitto Denko Corporation Aptamer capable of specifically adsorbing to bisphenol A and method for obtaining the aptamer
US20050079520A1 (en) * 2003-07-21 2005-04-14 Jie Wu Multiplexed analyte detection
KR100828481B1 (ko) * 2006-12-22 2008-05-13 고려대학교 산학협력단 환경호르몬에 특이적으로 결합하는 ｄｎａ 앱타머를 이용한환경호르몬 제거방법
US20110065086A1 (en) * 2008-02-21 2011-03-17 Otc Biotechnologies, Llc Methods of producing homogeneous plastic-adherent aptamer-magnetic bead-fluorophore and other sandwich assays

Also Published As

Publication number Publication date
EP3192881B1 (en) 2019-04-17
US9075053B2 (en) 2015-07-07
WO2010093223A3 (ko) 2011-02-24
WO2010093223A2 (ko) 2010-08-19
EP3495501B1 (en) 2022-10-05
EP2397562B1 (en) 2017-08-23
EP3192881A1 (en) 2017-07-19
EP3495501A1 (en) 2019-06-12
EP2397562A4 (en) 2012-08-15
CN102395684A (zh) 2012-03-28
ES2730808T3 (es) 2019-11-12
CN102395684B (zh) 2015-06-03
US20120040865A1 (en) 2012-02-16
EP2397562A2 (en) 2011-12-21

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