TR201907221T4 - G-csf üretimine yönelik yöntem. - Google Patents

G-csf üretimine yönelik yöntem. Download PDF

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Publication number
TR201907221T4
TR201907221T4 TR2019/07221T TR201907221T TR201907221T4 TR 201907221 T4 TR201907221 T4 TR 201907221T4 TR 2019/07221 T TR2019/07221 T TR 2019/07221T TR 201907221 T TR201907221 T TR 201907221T TR 201907221 T4 TR201907221 T4 TR 201907221T4
Authority
TR
Turkey
Prior art keywords
temperature
host cell
cultivation
recombinant polypeptide
csf
Prior art date
Application number
TR2019/07221T
Other languages
English (en)
Inventor
Felföldi Ferenc
Olasz Katalin
Kozma József
Original Assignee
Richter Gedeon Nyrt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Richter Gedeon Nyrt filed Critical Richter Gedeon Nyrt
Publication of TR201907221T4 publication Critical patent/TR201907221T4/tr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

İnklüzyon gövdelerinden rekombinant polipeptitlerin üretimi için yeni yöntemler açıklanmaktadır. Hücre kültür koşullarının modülasyonu, aktif formda rekombinant polipeptit verimini olumlu bir şekilde etkilemektedir. Bir yönde yöntem, (a) bir birinci sıcaklıkta söz konusu mikrobiyal bir konak hücrenin kültive edilmesini, konak hücre bir rekombinant polipeptidi kodlayan bir nükleik asit içermektedir, (b) kültivasyon sıcaklığının birinci sıcaklıktan bir ikinci sıcaklığa düşürülmesini, ve (c) ikinci sıcaklıkta konak hücrenin kültive edilmesini içermektedir.
TR2019/07221T 2012-03-19 2013-03-18 G-csf üretimine yönelik yöntem. TR201907221T4 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU1200171A HUP1200171A1 (hu) 2012-03-19 2012-03-19 Módszerek polipeptidek elõállítására

Publications (1)

Publication Number Publication Date
TR201907221T4 true TR201907221T4 (tr) 2019-06-21

Family

ID=89990648

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2019/07221T TR201907221T4 (tr) 2012-03-19 2013-03-18 G-csf üretimine yönelik yöntem.

Country Status (15)

Country Link
US (1) US9416164B2 (tr)
EP (2) EP2828396B1 (tr)
JP (1) JP6154885B2 (tr)
KR (1) KR101949556B1 (tr)
CN (1) CN104603280A (tr)
CA (1) CA2867794C (tr)
DK (1) DK2828396T3 (tr)
ES (1) ES2732374T3 (tr)
HR (1) HRP20190846T1 (tr)
HU (2) HUP1200171A1 (tr)
LT (1) LT2828396T (tr)
PL (1) PL2828396T3 (tr)
SI (1) SI2828396T1 (tr)
TR (1) TR201907221T4 (tr)
WO (1) WO2013068602A2 (tr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
US10435732B2 (en) 2014-06-09 2019-10-08 Kaneka Corporation Method for producing recombinant proteins using recombinant brevibacillus
WO2020140021A2 (en) * 2018-12-28 2020-07-02 Coherus Biosciences, Inc. Process for producing, isolating, and purifying modified recombinant proteins
WO2020234742A1 (en) * 2019-05-20 2020-11-26 Lupin Limited Granulocyte colony stimulating factor purification

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US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
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US5681720A (en) 1986-12-23 1997-10-28 Kyowa Hakko Co., Ltd. DNA encoding human granulocyte colony stimulating factor plasmids and host cells comprising same, and methods of expressing the encoded polypeptide
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CN101691560B (zh) * 2008-12-19 2011-08-31 华南理工大学 大肠杆菌及其可溶性表达转谷氨酰胺酶酶原的方法
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HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására

Also Published As

Publication number Publication date
KR20140144705A (ko) 2014-12-19
LT2828396T (lt) 2019-05-27
US20150044721A1 (en) 2015-02-12
EP2828396A2 (en) 2015-01-28
WO2013068602A2 (en) 2013-05-16
KR101949556B1 (ko) 2019-02-18
ES2732374T3 (es) 2019-11-22
CA2867794A1 (en) 2013-05-16
SI2828396T1 (sl) 2019-06-28
JP6154885B2 (ja) 2017-06-28
EP3517621A1 (en) 2019-07-31
JP2015514398A (ja) 2015-05-21
WO2013068602A3 (en) 2013-08-01
HUP1200171A1 (hu) 2013-09-30
CA2867794C (en) 2020-06-09
DK2828396T3 (da) 2019-05-27
HRP20190846T1 (hr) 2019-08-09
US9416164B2 (en) 2016-08-16
EP2828396B1 (en) 2019-04-24
CN104603280A (zh) 2015-05-06
HUE045310T2 (hu) 2019-12-30
PL2828396T3 (pl) 2019-08-30

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