TWI675041B - 用於純化抗體之連續多步驟方法 - Google Patents

用於純化抗體之連續多步驟方法 Download PDF

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Publication number
TWI675041B
TWI675041B TW107117528A TW107117528A TWI675041B TW I675041 B TWI675041 B TW I675041B TW 107117528 A TW107117528 A TW 107117528A TW 107117528 A TW107117528 A TW 107117528A TW I675041 B TWI675041 B TW I675041B
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Taiwan
Prior art keywords
chromatography
buffer
protein
chromatography column
passing
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TW107117528A
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English (en)
Chinese (zh)
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TW201908331A (zh
Inventor
多斯迪德
海默席琳
藍瑞克-伯丁羅瑞
莫斯班歐特
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法商賽諾菲公司
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Water Supply & Treatment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
TW107117528A 2013-05-06 2014-05-06 用於純化抗體之連續多步驟方法 TWI675041B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
??13305593.9 2013-05-06
EP13305593 2013-05-06

Publications (2)

Publication Number Publication Date
TW201908331A TW201908331A (zh) 2019-03-01
TWI675041B true TWI675041B (zh) 2019-10-21

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Family Applications (2)

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TW107117528A TWI675041B (zh) 2013-05-06 2014-05-06 用於純化抗體之連續多步驟方法
TW103116153A TWI631132B (zh) 2013-05-06 2014-05-06 用於純化抗體之連續多步驟方法

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TW103116153A TWI631132B (zh) 2013-05-06 2014-05-06 用於純化抗體之連續多步驟方法

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US (1) US10793622B2 (2)
EP (2) EP2994485B1 (2)
JP (2) JP6463734B2 (2)
KR (1) KR102272851B1 (2)
CN (2) CN105358572A (2)
AU (1) AU2014264697B2 (2)
BR (1) BR112015027812B1 (2)
CA (1) CA2911462C (2)
CY (1) CY1125281T1 (2)
DK (2) DK3301115T3 (2)
ES (2) ES2905675T3 (2)
HU (2) HUE057119T2 (2)
IL (1) IL242408B (2)
MX (1) MX368177B (2)
NO (1) NO2994485T3 (2)
RU (1) RU2662668C2 (2)
SG (1) SG11201508968XA (2)
TW (2) TWI675041B (2)
WO (1) WO2014180852A1 (2)

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Also Published As

Publication number Publication date
CA2911462A1 (en) 2014-11-13
SG11201508968XA (en) 2015-11-27
BR112015027812B1 (pt) 2021-03-30
JP2019104735A (ja) 2019-06-27
TWI631132B (zh) 2018-08-01
RU2662668C2 (ru) 2018-07-26
JP2016519137A (ja) 2016-06-30
JP6463734B2 (ja) 2019-02-06
EP2994485A1 (en) 2016-03-16
HUE057119T2 (hu) 2022-04-28
AU2014264697A1 (en) 2015-11-26
DK3301115T3 (da) 2022-01-31
EP3301115A1 (en) 2018-04-04
TW201908331A (zh) 2019-03-01
EP2994485B1 (en) 2018-01-10
CA2911462C (en) 2021-04-06
DK2994485T3 (en) 2018-04-23
ES2905675T3 (es) 2022-04-11
NO2994485T3 (2) 2018-06-09
AU2014264697B2 (en) 2019-08-22
MX2015015482A (es) 2016-03-21
RU2015151869A (ru) 2017-06-13
US10793622B2 (en) 2020-10-06
EP3301115B1 (en) 2021-11-10
TW201522363A (zh) 2015-06-16
BR112015027812A2 (pt) 2017-07-25
HUE038741T2 (hu) 2018-11-28
ES2665645T3 (es) 2018-04-26
CN105358572A (zh) 2016-02-24
WO2014180852A1 (en) 2014-11-13
MX368177B (es) 2019-09-23
KR20160005047A (ko) 2016-01-13
KR102272851B1 (ko) 2021-07-02
CN116003579A (zh) 2023-04-25
CY1125281T1 (el) 2023-06-09
IL242408B (en) 2020-04-30
US20160083454A1 (en) 2016-03-24

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