UA72552C2 - A method for production of heterologous proteinaceous substances in plant material - Google Patents

A method for production of heterologous proteinaceous substances in plant material Download PDF

Info

Publication number: UA72552C2
Authority: UA; Ukraine
Prior art keywords: protein; culture; moss; cells; mese
Prior art date: 1999-10-01

Application number

UA2002043701A

Other languages

English (en)

Ukrainian (uk)

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-10-01

Filing date

2000-09-27

Publication date

2005-03-15

2000-09-27 Application filed filed Critical

2005-03-15 Publication of UA72552C2 publication Critical patent/UA72552C2/uk

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Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

UA2002043701A 1999-10-01 2000-09-27 A method for production of heterologous proteinaceous substances in plant material UA72552C2 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE19947290A DE19947290A1 (de)	1999-10-01	1999-10-01	Verfahren zur Herstellung proteinöser Substanzen
PCT/DE2000/003374 WO2001025456A2 (de)	1999-10-01	2000-09-27	Verfahren zur herstellung proteinöser substanzen

Publications (1)

Publication Number	Publication Date
UA72552C2 true UA72552C2 (en)	2005-03-15

Family

ID=7924139

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
UA2002043701A UA72552C2 (en)	1999-10-01	2000-09-27	A method for production of heterologous proteinaceous substances in plant material

Country Status (31)

Country	Link
EP (1)	EP1206561B1 (is)
JP (1)	JP2003511035A (is)
KR (1)	KR100671217B1 (is)
CN (1)	CN1192111C (is)
AT (1)	ATE232904T1 (is)
AU (1)	AU781920B2 (is)
BG (1)	BG65487B1 (is)
BR (1)	BR0014671A (is)
CA (1)	CA2381995C (is)
CZ (1)	CZ293818B6 (is)
DE (2)	DE19947290A1 (is)
DK (1)	DK1206561T3 (is)
EE (1)	EE05093B1 (is)
ES (1)	ES2192545T3 (is)
HK (1)	HK1054053B (is)
HR (1)	HRP20020250B1 (is)
HU (1)	HU228206B1 (is)
IL (2)	IL148638A0 (is)
IS (1)	IS6299A (is)
MX (1)	MXPA02003359A (is)
NO (1)	NO329774B1 (is)
NZ (1)	NZ517996A (is)
PL (1)	PL200252B1 (is)
PT (1)	PT1206561E (is)
RS (1)	RS50075B (is)
RU (1)	RU2250264C2 (is)
SK (1)	SK287740B6 (is)
TR (1)	TR200200700T2 (is)
UA (1)	UA72552C2 (is)
WO (1)	WO2001025456A2 (is)
ZA (1)	ZA200202007B (is)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE60333201D1 (de) *	2002-09-12	2010-08-12	Greenovation Biotech Gmbh	Verfahren zur herstellung von proteinen
EP1398383A1 (en) *	2002-09-12	2004-03-17	greenovation Biotech GmbH	Protein production method
US7781197B2 (en)	2002-12-20	2010-08-24	Greenovation Biotech Gmbh	Transformed bryophyte cell having disrupted endogenous alpha 1,3-fucosyl and beta 1,2-xylosyl transferase encoding nucleotide sequences for the production of heterologous glycosylated proteins
DK1431394T3 (da) *	2002-12-20	2006-12-11	Greenovation Biotech Gmbh	Fremgangsmåde til fremstilling af heterologe, glycosylerede proteiner i mosplanteceller
EP1502954A1 (en)	2003-07-31	2005-02-02	greenovation Biotech GmbH	Utilisation of constructs comprising recombination sequence motifes for enhancing gene expression in moss
DE60326716D1 (de) *	2003-08-11	2009-04-30	Greenovation Biotech Gmbh	Promoter Sequenzen aus Moos und dessen Verwendung
DE602006015195D1 (de)	2005-07-12	2010-08-12	Greenovation Biotech Gmbh	Verbesserungen der proteinproduktion oder in zusammenhang damit
EP1905825A1 (en)	2006-09-29	2008-04-02	greenovation Biotech GmbH	Galactosyltransferase
KR200451866Y1 (ko) *	2008-07-28	2011-01-14	구동석	휴대폰 및 귀중품 보관함
RU2415936C1 (ru) *	2010-01-11	2011-04-10	Учреждение Российской академии наук Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН)	Способ секреции зеленого флуоресцентного белка (gfp) из растительных клеток
JP5641232B2 (ja) *	2010-11-24	2014-12-17	石川県公立大学法人	オゴノリ由来のシクロオキシゲナーゼの遺伝子及び該遺伝子を利用するプロスタグランジン類生産方法
EP2653548A1 (de)	2012-04-17	2013-10-23	Greenovation Biotech GmbH	Verfahren zur Erhöhung der Sekretion rekombinanter Proteine
RU2663131C1 (ru) *	2017-09-06	2018-08-01	федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ)	Носитель для культивирования клеток человека и животных
EP3808854A1 (de) *	2019-10-17	2021-04-21	eleva GmbH	Verfahren zur gewinnung von material von pflanzenzelloberflächen
CN115806930A (zh) *	2022-12-20	2023-03-17	河北师范大学	小反扭藓配子体的培养基及培养方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NL8901932A (nl) *	1989-07-26	1991-02-18	Mogen Int	Produktie van heterologe eiwitten in planten of plantecellen.
AU6504796A (en) *	1995-07-20	1997-02-18	Washington State University Research Foundation	Production of secreted foreign polypeptides in plant cell culture
DE19629402C2 (de) *	1996-07-20	1998-05-14	Dirk Dr Voeste	Verwendung von transformierten vaskulären Wasserpflanzen zur Produktion von arteigenen oder artfremden Substanzen
WO1998021348A1 (en) *	1996-11-12	1998-05-22	Battelle Memorial Institute	Method of producing human growth factors from whole plants or plant cell cultures
WO1998036085A1 (en) *	1997-02-13	1998-08-20	Applied Phytologics, Inc.	Production of mature proteins in plants
US6096546A (en) *	1998-01-30	2000-08-01	Board Of Trustees, Rutgers, The State University Of New Jersey	Methods for recovering polypeptides from plants and portions thereof

1999
- 1999-10-01 DE DE19947290A patent/DE19947290A1/de not_active Withdrawn
2000
- 2000-09-27 PL PL354716A patent/PL200252B1/pl unknown
- 2000-09-27 HK HK03106378.6A patent/HK1054053B/zh not_active IP Right Cessation
- 2000-09-27 CZ CZ20021061A patent/CZ293818B6/cs not_active IP Right Cessation
- 2000-09-27 HU HU0202628A patent/HU228206B1/hu unknown
- 2000-09-27 DE DE50001299T patent/DE50001299D1/de not_active Expired - Lifetime
- 2000-09-27 BR BR0014671-4A patent/BR0014671A/pt not_active Application Discontinuation
- 2000-09-27 ES ES00972602T patent/ES2192545T3/es not_active Expired - Lifetime
- 2000-09-27 PT PT00972602T patent/PT1206561E/pt unknown
- 2000-09-27 CA CA002381995A patent/CA2381995C/en not_active Expired - Lifetime
- 2000-09-27 NZ NZ517996A patent/NZ517996A/xx not_active IP Right Cessation
- 2000-09-27 WO PCT/DE2000/003374 patent/WO2001025456A2/de not_active Ceased
- 2000-09-27 KR KR1020027004246A patent/KR100671217B1/ko not_active Expired - Lifetime
- 2000-09-27 UA UA2002043701A patent/UA72552C2/uk unknown
- 2000-09-27 AU AU11295/01A patent/AU781920B2/en not_active Expired
- 2000-09-27 CN CNB008164967A patent/CN1192111C/zh not_active Expired - Lifetime
- 2000-09-27 AT AT00972602T patent/ATE232904T1/de active
- 2000-09-27 DK DK00972602T patent/DK1206561T3/da active
- 2000-09-27 RS YUP-246/02A patent/RS50075B/sr unknown
- 2000-09-27 MX MXPA02003359A patent/MXPA02003359A/es active IP Right Grant
- 2000-09-27 IL IL14863800A patent/IL148638A0/xx unknown
- 2000-09-27 TR TR2002/00700T patent/TR200200700T2/xx unknown
- 2000-09-27 SK SK415-2002A patent/SK287740B6/sk not_active IP Right Cessation
- 2000-09-27 HR HR20020250 patent/HRP20020250B1/xx not_active IP Right Cessation
- 2000-09-27 RU RU2002111666/13A patent/RU2250264C2/ru not_active IP Right Cessation
- 2000-09-27 EE EEP200200159A patent/EE05093B1/xx not_active IP Right Cessation
- 2000-09-27 JP JP2001528608A patent/JP2003511035A/ja active Pending
- 2000-09-27 EP EP00972602A patent/EP1206561B1/de not_active Expired - Lifetime
2002
- 2002-03-11 ZA ZA200202007A patent/ZA200202007B/en unknown
- 2002-03-12 IL IL148638A patent/IL148638A/en active IP Right Grant
- 2002-03-12 NO NO20021201A patent/NO329774B1/no not_active IP Right Cessation
- 2002-03-13 IS IS6299A patent/IS6299A/is unknown
- 2002-03-22 BG BG106547A patent/BG65487B1/bg unknown

Also Published As

Publication number	Publication date
BG106547A (en)	2003-04-30
CN1192111C (zh)	2005-03-09
NZ517996A (en)	2003-06-30
IS6299A (is)	2002-03-13
EE200200159A (et)	2003-04-15
PL354716A1 (en)	2004-02-09
BG65487B1 (bg)	2008-09-30
HK1054053B (zh)	2005-10-28
RU2002111666A (ru)	2004-03-10
NO329774B1 (no)	2010-12-13
EE05093B1 (et)	2008-10-15
AU781920B2 (en)	2005-06-23
HU228206B1 (en)	2013-01-28
BR0014671A (pt)	2002-07-23
DE19947290A1 (de)	2001-04-19
ES2192545T3 (es)	2003-10-16
KR100671217B1 (ko)	2007-01-18
MXPA02003359A (es)	2002-10-04
DE50001299D1 (de)	2003-03-27
EP1206561A2 (de)	2002-05-22
NO20021201L (no)	2002-05-08
RU2250264C2 (ru)	2005-04-20
IL148638A (en)	2008-12-29
IL148638A0 (en)	2002-09-12
ATE232904T1 (de)	2003-03-15
CA2381995C (en)	2010-01-05
CZ293818B6 (cs)	2004-08-18
KR20020065478A (ko)	2002-08-13
SK287740B6 (sk)	2011-08-04
SK4152002A3 (en)	2003-01-09
HUP0202628A2 (hu)	2002-12-28
HRP20020250A2 (en)	2004-02-29
YU24602A (sh)	2005-07-19
RS50075B (sr)	2009-01-22
JP2003511035A (ja)	2003-03-25
HK1054053A1 (en)	2003-11-14
PT1206561E (pt)	2003-06-30
WO2001025456A3 (de)	2001-12-27
DK1206561T3 (da)	2003-05-26
CA2381995A1 (en)	2001-04-12
AU1129501A (en)	2001-05-10
CZ20021061A3 (cs)	2002-07-17
PL200252B1 (pl)	2008-12-31
WO2001025456A2 (de)	2001-04-12
HUP0202628A3 (en)	2004-10-28
TR200200700T2 (tr)	2003-01-21
ZA200202007B (en)	2003-09-23
HRP20020250B1 (en)	2004-12-31
NO20021201D0 (no)	2002-03-12
CN1402791A (zh)	2003-03-12
EP1206561B1 (de)	2003-02-19

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