UA94399C2 - Мутантные промоторы аох1 - Google Patents

Мутантные промоторы аох1

Info

Publication number
UA94399C2
UA94399C2 UAA200710436A UAA200710436A UA94399C2 UA 94399 C2 UA94399 C2 UA 94399C2 UA A200710436 A UAA200710436 A UA A200710436A UA A200710436 A UAA200710436 A UA A200710436A UA 94399 C2 UA94399 C2 UA 94399C2
Authority
UA
Ukraine
Prior art keywords
nucleotides
mutant promoters
seq
aox1
pichia pastoris
Prior art date
Application number
UAA200710436A
Other languages
English (en)
Ukrainian (uk)
Inventor
Франц Хартнер
Антон Глідер
Original Assignee
Технише Университет Грац
ВТУ Холдинг ГмбХ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Технише Университет Грац, ВТУ Холдинг ГмбХ filed Critical Технише Университет Грац
Publication of UA94399C2 publication Critical patent/UA94399C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)

Abstract

Мутантный промотор алкогольоксидазы 1 (АОХ1) Pichia pastoris промотора AOX1 Pichia pastoris дикого типа (SEQ ID No. 1), который включает как минимум одну мутацию, выбранную из группы, которая состоит из: а) сайта связывания фактора транскрипции (TFBS), б) нуклеотидов 170-235 (от -784 до -719), нуклеотидов 192-213 (от -762 до -741), 192-210 (от -762 до -744), нуклеотидов 207-209 (от -747 до -745), нуклеотидов 214-235 (от -740 до -719), нуклеотидов 304-350 (от -650 до -604), нуклеотидов 364-393 (от -590 до -561), нуклеотидов 434-508 (от -520 до -446), нуклеотидов 509-551 (от -445 до -403), нуклеотидов 552-560 (от -402 до -394), нуклеотидов 585-617 (от -369 до -337), нуклеотидов 621-660 (от -333 до -294), нуклеотидов 625-683 (от -329 до -271), нуклеотидов 736-741 (от -218 до -213), нуклеотидов 737-738 (от -217 до -216), нуклеотидов 726-755 (от -228 до -199), нуклеотидов 784-800 (от -170 до -154) или нуклеотидов 823-861 (от -131 до -93) Seq ID No. 1 и их комбинации.
UAA200710436A 2005-02-23 2006-02-23 Мутантные промоторы аох1 UA94399C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0030405A AT501955B1 (de) 2005-02-23 2005-02-23 Mutierte aox1-promotoren

Publications (1)

Publication Number Publication Date
UA94399C2 true UA94399C2 (ru) 2011-05-10

Family

ID=36927772

Family Applications (2)

Application Number Title Priority Date Filing Date
UAA201012563A UA98604C2 (ru) 2005-02-23 2006-02-23 Мутантные промоторы аох1
UAA200710436A UA94399C2 (ru) 2005-02-23 2006-02-23 Мутантные промоторы аох1

Family Applications Before (1)

Application Number Title Priority Date Filing Date
UAA201012563A UA98604C2 (ru) 2005-02-23 2006-02-23 Мутантные промоторы аох1

Country Status (20)

Country Link
US (2) US9012175B2 (ru)
EP (2) EP1851312B9 (ru)
JP (2) JP5497986B2 (ru)
KR (2) KR101352399B1 (ru)
CN (2) CN101935657B (ru)
AT (3) AT501955B1 (ru)
AU (2) AU2006216094B2 (ru)
BR (1) BRPI0606179B1 (ru)
CA (2) CA2598514C (ru)
DE (2) DE602006019789D1 (ru)
DK (2) DK2199389T3 (ru)
ES (2) ES2358086T3 (ru)
HR (2) HRP20110084T2 (ru)
IN (1) IN2012DN06694A (ru)
NZ (2) NZ560542A (ru)
PL (2) PL1851312T3 (ru)
PT (1) PT1851312E (ru)
SI (2) SI2199389T1 (ru)
UA (2) UA98604C2 (ru)
WO (1) WO2006089329A2 (ru)

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AU2009228322B2 (en) * 2008-03-27 2013-09-26 Alliance For Sustainable Energy, Llc High expression Zymomonas promoters
CN102105589A (zh) * 2008-07-11 2011-06-22 诺维信公司 巴斯德毕赤酵母(Pichia pastoris) DAS启动子变体
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
US20140011236A1 (en) * 2011-03-22 2014-01-09 Merch Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
GB201113419D0 (en) * 2011-08-04 2011-09-21 Fujifilm Diosynth Biotechnologies Uk Ltd Yeast vector
EP2565262A1 (en) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
KR101954389B1 (ko) * 2011-10-07 2019-03-06 론자 리미티드 조절가능한 프로모터
WO2014088563A1 (en) * 2012-12-05 2014-06-12 Exxonmobil Research And Engineering Company Nannochloropsis kozak consensus sequence
EP3839055B1 (en) 2013-03-08 2023-08-23 Biogrammatics, Inc. Yeast promoters for protein expression
US10619164B2 (en) 2013-03-08 2020-04-14 Keck Graduate Institute Of Applied Life Sciences Yeast promoters from Pichia pastoris
CN105229154B (zh) 2013-03-15 2026-03-03 龙沙有限公司 组成型启动子
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
EP3217807A4 (en) 2014-11-11 2018-09-12 Clara Foods Co. Methods and compositions for egg white protein production
CN107614516A (zh) 2015-05-11 2018-01-19 非凡食品有限公司 用于基因工程改造甲基营养型酵母的表达构建体和方法
US10752907B2 (en) 2015-08-05 2020-08-25 Lonza Ltd Promoter variants
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
AU2018291000B2 (en) * 2017-06-30 2022-10-27 Dow Global Technologies Llc Coating for aldehyde remediation and method of making
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
JP2022502018A (ja) * 2018-09-24 2022-01-11 オルタ ドグ テクニク ユニヴェルシテシ 改変されたａｏｘ１プロモータバリアント
WO2020068019A2 (en) 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering
CN109735547B (zh) * 2019-03-19 2022-05-31 青岛蔚蓝生物集团有限公司 一种提高毕赤酵母外源蛋白表达量的启动子及其应用
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
WO2020215017A1 (en) * 2019-04-17 2020-10-22 Impossible Foods Inc. Materials and methods for protein production
CN110106168B (zh) * 2019-05-27 2021-07-13 西南大学 基于转录因子间的互作关系构建双激素响应启动子的方法
SI3997118T1 (sl) 2019-07-11 2025-06-30 Clara Foods Co. Sestavek napitka, ki obsega rekombinantni ovomukoidni protein
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN110724720A (zh) * 2019-11-01 2020-01-24 山东仙普爱瑞科技股份有限公司 一种猪α-干扰素的发酵工艺
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
KR20230144629A (ko) 2021-02-12 2023-10-16 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 단백질 분비 증가를 위한 신호 펩타이드
US11512336B1 (en) * 2021-06-24 2022-11-29 BioBoost Synbio Consulting Inc. Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products
WO2024253122A1 (ja) 2023-06-06 2024-12-12 富士フイルム株式会社 遺伝子組換え酵母、目的タンパク質の製造方法、キットおよび遺伝子組換え酵母の製造方法
EP4728077A1 (en) * 2023-06-15 2026-04-22 Danstar Ferment AG C2 carbon source-responsive promoters
CN117417934B (zh) * 2023-10-27 2024-09-13 江南大学 跨菌种启动子、多菌种穿梭质粒及其应用
WO2025104126A1 (en) 2023-11-14 2025-05-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
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WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN118599913B (zh) * 2024-08-07 2024-12-27 南昌大学 醛氧化酶Aox1基因敲除动物模型的构建方法及应用

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CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
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MXPA03009059A (es) * 2001-04-05 2004-10-15 Univ Nebraska Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura.
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Also Published As

Publication number Publication date
AU2011200187B2 (en) 2012-01-19
AT501955A1 (de) 2006-12-15
BRPI0606179B1 (pt) 2018-05-29
DK1851312T3 (da) 2011-02-28
ES2358086T3 (es) 2011-05-05
ES2356127T9 (es) 2011-05-30
JP5497986B2 (ja) 2014-05-21
CA2785161A1 (en) 2006-08-31
EP1851312A2 (en) 2007-11-07
CA2785161C (en) 2020-03-31
ATE487788T1 (de) 2010-11-15
DK1851312T5 (da) 2011-04-18
DK2199389T3 (da) 2011-04-11
DE602006018113D1 (de) 2010-12-23
ES2356127T3 (es) 2011-04-05
NZ560542A (en) 2010-06-25
US9012175B2 (en) 2015-04-21
KR101352399B1 (ko) 2014-02-18
PT1851312E (pt) 2011-01-05
HRP20110084T1 (hr) 2011-03-31
DE602006019789D1 (de) 2011-03-03
HK1113172A1 (en) 2008-09-26
CN101128581A (zh) 2008-02-20
WO2006089329A2 (en) 2006-08-31
AT501955B1 (de) 2007-08-15
ATE496125T1 (de) 2011-02-15
CN101935657B (zh) 2013-02-13
KR20070107781A (ko) 2007-11-07
SI2199389T1 (sl) 2011-05-31
PL2199389T3 (pl) 2011-06-30
EP1851312B9 (en) 2011-04-20
WO2006089329A8 (en) 2006-11-02
AU2006216094A1 (en) 2006-08-31
KR101358663B1 (ko) 2014-02-20
HRP20110254T1 (hr) 2011-05-31
EP2199389B1 (en) 2011-01-19
CA2598514C (en) 2013-07-09
JP2012080886A (ja) 2012-04-26
BRPI0606179A2 (pt) 2009-06-02
SI1851312T1 (sl) 2011-03-31
AU2011200187A1 (en) 2011-02-03
KR20120106979A (ko) 2012-09-27
CA2598514A1 (en) 2006-08-31
US9279129B2 (en) 2016-03-08
CN101935657A (zh) 2011-01-05
UA98604C2 (ru) 2012-05-25
EP2199389A1 (en) 2010-06-23
JP5552107B2 (ja) 2014-07-16
PL1851312T3 (pl) 2011-05-31
US20100196913A1 (en) 2010-08-05
WO2006089329A3 (en) 2007-05-31
NZ585821A (en) 2011-02-25
CN101128581B (zh) 2012-04-18
US20080153126A1 (en) 2008-06-26
HRP20110084T2 (hr) 2011-10-31
JP2008531000A (ja) 2008-08-14
IN2012DN06694A (ru) 2015-10-23
AU2006216094B2 (en) 2010-10-21
EP1851312B1 (en) 2010-11-10
HK1148313A1 (en) 2011-09-02

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