US20170137792A1 - Recombinant polynucleotide sequence for producing astaxanthin and uses thereof - Google Patents

Recombinant polynucleotide sequence for producing astaxanthin and uses thereof Download PDF

Info

Publication number
US20170137792A1
US20170137792A1 US15/353,372 US201615353372A US2017137792A1 US 20170137792 A1 US20170137792 A1 US 20170137792A1 US 201615353372 A US201615353372 A US 201615353372A US 2017137792 A1 US2017137792 A1 US 2017137792A1
Authority
US
United States
Prior art keywords
gene
seq
amino acid
promoter
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US15/353,372
Other languages
English (en)
Inventor
Jui-Jen Chang
Caroline THIA
Hao-Yeh Lin
Yu-Ju Lin
Chieh-Chen Huang
Wen-Hsiung Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Academia Sinica
Original Assignee
Academia Sinica
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Academia Sinica filed Critical Academia Sinica
Priority to US15/353,372 priority Critical patent/US20170137792A1/en
Assigned to ACADEMIA SINICA reassignment ACADEMIA SINICA ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHANG, JUI-JEN, HUANG, CHIEH-CHEN, LI, WEN-HSIUNG, LIN, Hao-Yeh, LIN, YU-JU, THIA, Caroline
Publication of US20170137792A1 publication Critical patent/US20170137792A1/en
Priority to US16/200,269 priority patent/US10865397B2/en
Priority to US17/094,623 priority patent/US11781124B2/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • FIG. 17C is a line chart that depicts the growth curve of specified strains according to one example of the present disclosure.
  • FIG. 24F presents the HPLC data of the astaxanthin produced by CA6-ITS strain according to one example of the present disclosure
  • FIG. 29A are photographs that reveal the colonies of wild-type and Cz30 strains respectively treated with specified concentration of 10 deacetylbaccatin III (10 DB), which is dissolved in specified concentration of ethanol, according to one example of the present disclosure;
  • promoter is to describe a synthetic or fusion molecule or derivative, which confers, activates or enhances expression of a nucleic acid sequence in a cell, tissue or organ.
  • the first promoter is the ScPGK promoter
  • the second promoter is the ScADHI promoter
  • the third promoter is the KILac4 promoter
  • the fourth promoter is the KIADHI promoter
  • the marker promoter is the KIGapDH promoter.
  • the fifth gene cassette comprises a fifth nucleic acid sequence driven by a fifth promoter, in which the fifth nucleic acid sequence encodes a ⁇ -carotene hydroxylase.
  • the sixth gene cassette comprises a sixth nucleic acid sequence driven by a sixth promoter, in which the sixth nucleic acid sequence encodes a ⁇ -carotene ketolase.
  • the present recombinant polynucleotide sequence and/or vector can be introduced into the host cell by the method known to a skilled artisan.
  • the method for introducing the exogenous DNA and/or vector into a prokaryotic host cell includes chemical treatment (such as incubating the host cell in a solution containing divalent cations, then followed by a heat treatment), and electroporation (such as briefly treating the host cell with an electric field that creates holes in the cell membrane).
  • the thus produced astaxanthin, a precursor, and/or a derivative thereof exhibits anti-oxidation activity that renders the host cell tolerant to a stress.
  • the stress may be caused from the host cell being exposed to ethanol, butanol, UV exposure, furfural, and/or the precursor of an anticancer drug.
  • the host cell is tolerant to a precursor of an anticancer drug, 10-deacetyl baccatin III (10 DB), which gives rise to the anticancer drug, paclitaxel.
  • the tolerance of the host cell to different stresses can be evaluated by various methods, depending on the experimental purpose. For example, the tolerance can be evaluated by the measurement of colony number, cell growth, cell density, or gene expression.
  • the medium may comprises at least two components selected from the group consisting of glucose, galactose, glycerol, and fatty acid so as to efficiently induce the expression of gene cassettes within the hose cell.
  • the synthesized bkt gene had an amino acid sequence of SEQ ID NO: 8; the synthesized CrChYb gene had an amino acid sequence of SEQ ID NO: 5; the synthesized CzChYb gene had an amino acid sequence of SEQ ID NO: 6; and the synthesized HpChYb gene had an amino acid sequence of SEQ ID NO: 7.
  • the engineered yeasts were cultivated in YPG medium (1 BactoDifco-Yeast Extract, 2% BactoDifco-Peptone, 2% Merck-D(+)-Galactose) respectively at 25° C., 30° C., and 37° C. for 3 days.
  • the engineered yeasts were cultivated in YPG medium with the addition of 20% glucose, 20% galactose, or 20% glycerol.
  • the electroporation was performed (1.0 kV, 400 ⁇ , and 25 ⁇ F capacitance) using a BioRad system (GenePluserXcellTM, Bio-Rad, Hercules, Calif.) with an aluminum cuvette (2 mm).
  • the cells were spread onto YPG plates (1% BactoDifco-Yeast Extract, 2% BactoDifco-Peptone, and 2% Merck-galactose) containing G418 (200 ⁇ g/ml).
  • a recombinant polynucleotide sequence crtE-Kan-tHMG1 was constructed to comprise a crtE gene and a HMG1 gene.
  • the neomycin phosphotransferase gene essential for G418 resistance (Kan) was used as a marker gene for clone screening.
  • the Cz30 cell cultivated in glycerol which is the byproduct of the bio-diesel industry, exhibited a significant red color change, as compared with culturing in other carbon sources, indicating the potential use of Cz30 for the development of a green industry.
  • the broth of Cz30 remained in red color after being cultured for 5 days in 20% glycerol, although the relationship between glycerol metabolism and carotenoid esterification is still not clear.
  • the carotenoids were extracted from WT and Cz30 strain, and the antioxidant ability of the thus produced carotenoids was determined by use of the ABTS substrate reaction. After culturing in YPG medium at 25° C. for 72 hours, the host cells were lyophilized and the pigments in the cells were extracted by methanol. The extract of Cz30 exhibited a higher free radical scavenging capacity (72.1%) than that of WT (52.3%) ( FIG. 23A ).
  • the astaxanthin synthesis gene cassettes were introduced together with a high copy expression plasmid RS426 to the CA6-plasmid strain ( FIG. 24D ).
  • the gene cassettes would be spontaneously assembled to the plasmid in vivo; and the transformant could produce orange to red colonies.
  • the host cell With the high copy number of plasmids, the host cell has potential to express high amounts of proteins, and to convert the precursor to astaxanthin more efficiently.
  • the data of FIG. 23 revealed that the cell extract of Cz30 exhibited an antioxidant activity in comparison to that of wild-type (WT) control.
  • WT wild-type
  • the anti-stress capability of Cz30 was further confirmed by UV, furfural, ethanol, or isobutanol treatment.
  • the WT and Cz30 were separately exposed to UV for 5, 10, or 20 minutes, and then inoculated in the YPG plate with a series dilution and cultivated for 48 hours. Only some Cz30 colonies could grow on the YPG plate after being exposed to UV light for 20 minutes ( FIG. 27A ). This observation suggested that the carotenoid products of Cz30 could reduce UV damage, resulting in faster cell growth as compared to that of the WT.
  • Toxins produced during acid and steam pretreatment of lignocellulose cover a large range of substances, such as furfural and hydroxymethylfurfural from hemicellulose and cellulose, alcohols and aldehydes from lignin, and heavy metals from bioreactor. Accordingly, the second stress factor examined was furfural treatment.
  • the Cz30 strain could tolerate the treatment of 100 mM furfural, whereas the WT strain could only tolerate the treatment with 80 mM furfural.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Epoxy Compounds (AREA)
  • Cosmetics (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
US15/353,372 2014-05-16 2016-11-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof Abandoned US20170137792A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US15/353,372 US20170137792A1 (en) 2014-05-16 2016-11-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US16/200,269 US10865397B2 (en) 2014-05-16 2018-11-26 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US17/094,623 US11781124B2 (en) 2014-05-16 2020-11-10 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461994828P 2014-05-16 2014-05-16
PCT/US2015/031273 WO2015176054A2 (en) 2014-05-16 2015-05-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US15/353,372 US20170137792A1 (en) 2014-05-16 2016-11-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/031273 Continuation WO2015176054A2 (en) 2014-05-16 2015-05-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US16/200,269 Continuation US10865397B2 (en) 2014-05-16 2018-11-26 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Publications (1)

Publication Number Publication Date
US20170137792A1 true US20170137792A1 (en) 2017-05-18

Family

ID=54480945

Family Applications (3)

Application Number Title Priority Date Filing Date
US15/353,372 Abandoned US20170137792A1 (en) 2014-05-16 2016-11-16 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US16/200,269 Active US10865397B2 (en) 2014-05-16 2018-11-26 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US17/094,623 Active 2036-10-03 US11781124B2 (en) 2014-05-16 2020-11-10 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Family Applications After (2)

Application Number Title Priority Date Filing Date
US16/200,269 Active US10865397B2 (en) 2014-05-16 2018-11-26 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US17/094,623 Active 2036-10-03 US11781124B2 (en) 2014-05-16 2020-11-10 Recombinant polynucleotide sequence for producing astaxanthin and uses thereof

Country Status (8)

Country Link
US (3) US20170137792A1 (de)
EP (2) EP3460044B1 (de)
JP (2) JP2017515512A (de)
CN (2) CN111500599A (de)
CA (2) CA3109156C (de)
CL (1) CL2016002906A1 (de)
TW (1) TWI681052B (de)
WO (1) WO2015176054A2 (de)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11124819B2 (en) * 2017-03-15 2021-09-21 The Regents Of The University Of California Genes involved in astaxanthin biosynthesis
CN114410523A (zh) * 2022-01-20 2022-04-29 中国农业科学院茶叶研究所 一种高效制备红茶菌的菌种组合及其应用
US20230159941A1 (en) * 2019-01-29 2023-05-25 Clariant Produkte (Deutschland) Gmbh Chimeric Promoter For Use In Metabolic Engineering

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110283867A (zh) * 2019-05-24 2019-09-27 华南理工大学 一种利用佐夫色绿藻生产虾青素的方法
WO2024151157A2 (en) * 2023-01-10 2024-07-18 Universiti Putra Malaysia Astaxanthin-expressing yeast as feed additive for aquaculture
CN117604060A (zh) * 2024-01-24 2024-02-27 逢时(青岛)海洋科技有限公司 一种基于固定化胆固醇酯酶的游离虾青素及其制备方法

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2216027T3 (es) * 1995-06-09 2004-10-16 Dsm Ip Assets B.V. Produccion de carotenoides fermentativos.
DE69633343T2 (de) * 1995-12-22 2005-09-22 Dsm Ip Assets B.V. Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar
DE60005871T2 (de) * 1999-03-09 2004-05-06 Dsm Ip Assets B.V. Astaxanthin Synthetase
DE19916140A1 (de) * 1999-04-09 2000-10-12 Basf Ag Carotinhydroxylase und Verfahren zur Herstellung von Xanthophyllderivaten
JP2004033070A (ja) * 2002-07-01 2004-02-05 Yamaha Motor Co Ltd 緑藻ヘマトコッカスへの外来遺伝子導入方法
DE10253112A1 (de) * 2002-11-13 2004-06-03 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in genetisch veränderten Organismen
DE10238980A1 (de) * 2002-08-20 2004-03-04 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von Ketocarotinoiden in Blütenblättern von Pflanzen
DE10258971A1 (de) * 2002-12-16 2004-07-01 Sungene Gmbh & Co. Kgaa Verwendung von astaxanthinhaltigen Pflanzen oder Pflanzenteilen der Gattung Tagetes als Futtermittel
DE10300649A1 (de) * 2003-01-09 2004-07-22 Basf Ag Verfahren zur Herstellung von Ketocarotinoiden durch Kultivierung von genetisch veränderten Organismen
DE102004007624A1 (de) 2004-02-17 2005-09-15 Sungene Gmbh & Co. Kgaa Neue Ketolasen und Verfahren zur Herstellung von Ketocarotinoiden
CN103589650A (zh) * 2005-03-18 2014-02-19 米克罗比亚公司 产油酵母和真菌中类胡萝卜素的产生
US20070157339A1 (en) * 2006-01-04 2007-07-05 Cunningham Francis X Jr Biochemical route to astaxanthin
US8373023B2 (en) * 2006-04-21 2013-02-12 University Of Maryland Biochemical route to astaxanthin
CN101516906B (zh) * 2006-07-21 2013-11-06 诺维信股份有限公司 提高具有生物学活性之多肽的分泌的方法
EP2048939A4 (de) 2006-08-17 2010-04-28 Monsanto Technology Llc Transgene pflanzen mit erweiterten agronomischen merkmalen
WO2008042338A2 (en) * 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7935156B2 (en) * 2006-11-21 2011-05-03 Amyris Biotechnologies, Inc. Jet fuel compositions and methods of making and using same
US8846374B2 (en) * 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
WO2008148131A1 (en) * 2007-05-31 2008-12-04 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CL2008001699A1 (es) * 2008-06-09 2010-02-05 Univ Chile Secuencia de adn que codifica enzima con actividad citocromo p450 reductasa de x. dendrorhous; secuencia de polipeptido codificada; vector o plasmido; celula hesped; proceso de produccion de polipeptido; proceso de produccion de astaxantina desde beta-caroteno; composiciones, productos y/o formulaciones.
EP2199399A1 (de) * 2008-12-17 2010-06-23 BASF Plant Science GmbH Ketokarotinoidproduktion in Pflanzen
WO2010079032A1 (en) * 2008-12-17 2010-07-15 Basf Plant Science Gmbh Production of ketocarotenoids in plants
WO2011146833A1 (en) * 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
IN2014CN01129A (de) * 2011-08-08 2015-04-10 Evolva Sa
AU2012359983B2 (en) * 2011-12-28 2018-04-12 Evogene Ltd. Isolated polynucleotides and polypeptides, and methods of using same for increasing yield of plants
CN102888425B (zh) * 2012-07-02 2015-02-25 中国科学院昆明植物研究所 利用转基因植物生产虾青素的方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11124819B2 (en) * 2017-03-15 2021-09-21 The Regents Of The University Of California Genes involved in astaxanthin biosynthesis
US20230159941A1 (en) * 2019-01-29 2023-05-25 Clariant Produkte (Deutschland) Gmbh Chimeric Promoter For Use In Metabolic Engineering
CN114410523A (zh) * 2022-01-20 2022-04-29 中国农业科学院茶叶研究所 一种高效制备红茶菌的菌种组合及其应用

Also Published As

Publication number Publication date
TWI681052B (zh) 2020-01-01
EP3143121A4 (de) 2018-02-28
CA3109156A1 (en) 2015-11-19
CA3109156C (en) 2023-08-15
CN111500599A (zh) 2020-08-07
CA2949381A1 (en) 2015-11-19
WO2015176054A2 (en) 2015-11-19
EP3460044A1 (de) 2019-03-27
CL2016002906A1 (es) 2018-03-16
CN106795477A (zh) 2017-05-31
JP2019150047A (ja) 2019-09-12
US10865397B2 (en) 2020-12-15
US11781124B2 (en) 2023-10-10
US20190144841A1 (en) 2019-05-16
US20210071153A1 (en) 2021-03-11
WO2015176054A3 (en) 2016-01-07
EP3143121A2 (de) 2017-03-22
JP6964622B2 (ja) 2021-11-10
TW201544593A (zh) 2015-12-01
JP2017515512A (ja) 2017-06-15
EP3460044B1 (de) 2024-01-24

Similar Documents

Publication Publication Date Title
US11781124B2 (en) Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
D'Adamo et al. Engineering the unicellular alga Phaeodactylum tricornutum for high‐value plant triterpenoid production
Lin et al. Metabolic engineering a yeast to produce astaxanthin
Xiao et al. The c4h, tat, hppr and hppd genes prompted engineering of rosmarinic acid biosynthetic pathway in Salvia miltiorrhiza hairy root cultures
CN103827304B (zh) 用于异丁醇生产的宿主细胞和方法
DE112015003962B4 (de) 3-Hydroxypropionsäure erzeugende rekombinante Hefe und Verfahren zum Herstellen von 3-Hydroxypropionsäure unter Nutzung derselben
Chang et al. Integrating an algal β-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast
Liu et al. Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin
Lin et al. Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis
KR102170444B1 (ko) 세포 소기관이 변이된 재조합 효모 및 이를 이용한 아이소프레노이드 생산 방법
Lou et al. Molecular cloning and functional characterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris
Naz et al. Genetic modification of Mucor circinelloides for canthaxanthin production by heterologous expression of β-carotene ketolase gene
Wang et al. Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica
JP2021525085A (ja) メタクリル酸エステルの産生の方法
Krishna et al. A metabolic-engineering framework approach via fed-batch fermentation for enhancing glucaric acid production in Komagataella phaffii
Song et al. Overexpression of the KAS Ⅲ-like gene YxwZ3 increases carotenoids production in Aurantiochytrium sp. SZU445
Fossati et al. L-ascorbic acid producing yeasts learn from plants how to recycle it
JP7412753B2 (ja) α－リノレン酸産生能が向上した微生物またはその培養物
Han et al. CRISPR-Cas9 mediated construction of a food-grade pyrroloquinoline quinone high-yielding Acetobacter pasteurianus Ab3 strain
EA035512B1 (ru) Промоторы, подходящие для экспрессии гетерологичных генов в дрожжах
Dong et al. De Novo Biosynthesis of Violacein Utilizing Saccharomyces cerevisiae as a Cell Factory
Zhu et al. Reprogramming lipid metabolism and morphology engineering in Yarrowia lipolytica for algae-free, high-titer production of esterified astaxanthin
KR20240041434A (ko) 효모에서 폴리케티드를 제조하기 위한 방법
Zheng et al. Transgenic approaches to boost bromoform production in Pyropia yezoensis for application as anti-methanogenic feed additive
Ganesan Developing Next-Generation Tools for Metabolic Engineering in Non-conventional Yeast Yarrowia lipolytica

Legal Events

Date Code Title Description
AS Assignment

Owner name: ACADEMIA SINICA, TAIWAN

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:CHANG, JUI-JEN;THIA, CAROLINE;LIN, HAO-YEH;AND OTHERS;REEL/FRAME:041123/0172

Effective date: 20161117

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION