US7767297B2 - Fiber, fiber assembly, and fiber producing method - Google Patents

Fiber, fiber assembly, and fiber producing method Download PDF

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US7767297B2
US7767297B2 US12/181,560 US18156008A US7767297B2 US 7767297 B2 US7767297 B2 US 7767297B2 US 18156008 A US18156008 A US 18156008A US 7767297 B2 US7767297 B2 US 7767297B2
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fiber
eggshell membrane
fiber according
solution
membrane component
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US20090031691A1 (en
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Takeharu Tajima
Nobuo Kusamoto
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Biovotec As
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Idemitsu Technofine Co Ltd
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Assigned to IDEMITSU KOSAN CO., LTD. reassignment IDEMITSU KOSAN CO., LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: IDEMITSU TECHNOFINE CO., LTD.
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0208—Tissues; Wipes; Patches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0241—Containing particulates characterized by their shape and/or structure
    • A61K8/027—Fibers; Fibrils
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/982—Reproductive organs; Embryos, Eggs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q15/00—Anti-perspirants or body deodorants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
    • D01D5/0038—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion the fibre formed by solvent evaporation, i.e. dry electro-spinning
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/28—Formation of filaments, threads, or the like while mixing different spinning solutions or melts during the spinning operation; Spinnerette packs therefor
    • D01D5/30—Conjugate filaments; Spinnerette packs therefor
    • D01D5/34—Core-skin structure; Spinnerette packs therefor
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F1/00—General methods for the manufacture of artificial filaments or the like
    • D01F1/02—Addition of substances to the spinning solution or to the melt
    • D01F1/10—Other agents for modifying properties
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2922—Nonlinear [e.g., crimped, coiled, etc.]
    • Y10T428/2924—Composite
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2927—Rod, strand, filament or fiber including structurally defined particulate matter
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2929—Bicomponent, conjugate, composite or collateral fibers or filaments [i.e., coextruded sheath-core or side-by-side type]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2913—Rod, strand, filament or fiber
    • Y10T428/2929—Bicomponent, conjugate, composite or collateral fibers or filaments [i.e., coextruded sheath-core or side-by-side type]
    • Y10T428/2931—Fibers or filaments nonconcentric [e.g., side-by-side or eccentric, etc.]

Definitions

  • the present invention relates to a fiber containing an eggshell membrane component, a fiber assembly, and a fiber producing method.
  • an eggshell membrane can be effectively used as a wound dressing. It is because the eggshell membrane has cell-producing action of amino acid, adherence to texture due to a network structure thereof, appropriate moisture retention, air permeability and the like, which are suitable for the wound dressing.
  • an extractable eggshell membrane varies in size depending on egg size and is not flattened in shape, adjustment of the extractable eggshell membrane to the shape of a wound portion has been difficult. Accordingly, it has been desired to industrially obtain a sheet-like or a film-like dressing consisting of or containing an eggshell membrane component.
  • the film or the sheet disclosed in Documents 1 and 2 does not have a network structure of a natural eggshell membrane. Also, its moisture retention and air permeability, and adherence to texture are not sufficient for using the film or the sheet as the wound dressing or a cosmetic sheet. Further, the fiber treatment agent disclosed in Document 3 does not have enough quality to imitate a structure of the eggshell membrane and a content of the eggshell membrane cannot be easily raised.
  • An object of the present invention is to provide a fiber that imitates an eggshell membrane structure using an eggshell membrane component, a fiber assembly and a fiber producing method.
  • the present invention provides the fiber, the fiber assembly and the fiber producing method as described below.
  • the fiber assembly formed from the fiber has substantially the same properties of a natural eggshell membrane since the fiber itself contains the eggshell membrane component.
  • the fiber assembly according to the present invention has sufficient moisture retention, air permeability by imitating a network structure of the natural eggshell membrane.
  • the fiber assembly since the fiber assembly is excellent in adherence to skin tissue of a human body and stypticity, the fiber assembly may be preferably used as a wound dressing, a cosmetic sheet or the like.
  • a shape and an area of the fiber assembly are not limited, unfavorable features of the nature eggshell membrane that an extractable eggshell membrane varies in size depending on egg size and is not flattened in shape are eliminated.
  • the fiber containing the eggshell membrane can be easily obtained by spinning the solution containing the eggshell membrane component by employing the electropinning method.
  • the fiber assembly can be simultaneously produced with the fiber.
  • FIG. 1 is a perspective view showing an outline of a producing device for a fiber producing method according to an exemplary embodiment of the present invention.
  • FIG. 2 is a cross sectional view of FIG. 1 .
  • FIG. 3 is a schematic diagram showing a producing device (nozzle type) for a fiber producing method according to another exemplary embodiment of the present invention.
  • FIG. 4A is a schematic diagram showing a producing device (core/sheath nozzle type) for a fiber producing method according to still another exemplary embodiment of the present invention.
  • FIG. 4B is another schematic diagram showing the producing device (core/sheath nozzle type) for the fiber producing method according to the still another exemplary embodiment of the present invention.
  • FIG. 5A shows a scanning electron micrograph of a fiber assembly after spinning according to an example 1.
  • FIG. 5B shows another scanning electron micrograph of the fiber assembly after spinning according to the example 1.
  • FIG. 6 shows a scanning electron micrograph of a fiber assembly after spinning according to an example 2.
  • FIG. 7 shows a scanning electron micrograph of a fiber assembly after spinning according to an example 3.
  • FIG. 8 shows another scanning electron micrograph of the fiber assembly (after oxidation and cross-linking) after spinning according to the example 3.
  • FIG. 9 shows a scanning electron micrograph of a fiber assembly after spinning according to an example 4.
  • FIG. 10 shows a scanning electron micrograph of a fiber assembly after spinning according to an example 5.
  • a fiber and a fiber assembly according to the present invention contain an eggshell membrane component.
  • the fiber itself contains the eggshell membrane component.
  • FIGS. 5A and 5B show scanning electron micrographs as examples of the fiber assembly according to the present invention. It should be understood from the micrographs that the fiber assembly imitates a network structure of a natural eggshell membrane.
  • the fiber assembly is used as, for example, a wound dressing.
  • the fiber and the fiber assembly according to the present invention may additionally contain polyphenols.
  • the polyphenols include polyhydric phenols such as catechins.
  • the catechins are extracted from a tea leaf, persimmon tannin, bark, cacao bean or the like.
  • Examples of the catechins include epicatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate and attributes thereof.
  • the eggshell membrane component is preferably contained in the fiber with a content of 20 mass %, more preferably 50 mass %, most preferably substantially 100 mass %.
  • content of the eggshell membrane component is less than 20 mass %, features of the natural eggshell membrane such as cell-producing action and biodegradability are not sufficiently retained.
  • a fiber diameter according to the present invention is preferably between and including 0.01 ⁇ m and 3 ⁇ m, more preferably between and including 0.1 ⁇ m and 1.8 ⁇ m. With such diameter range, a surface area of the fiber assembly can be sufficiently enlarged, and appropriate moisture retention, air permeability and filter effect of the natural eggshell membrane can be exhibited. However, the fiber diameter less than 0.01 ⁇ m is not preferable because it may be difficult to produce the fiber with such diameter and the productivity may be lowered.
  • the above-described fiber and fiber assembly according to the present invention can be produced by employing an electrospinning method (i.e., electrolytic spinning, electrostatic spinning) after pulverizing the eggshell membrane to form a solution.
  • an electrospinning method i.e., electrolytic spinning, electrostatic spinning
  • the eggshell membrane according to the present invention is preferably obtained from chicken eggs in view of availability, but it is not limited to chicken eggs.
  • the eggshell membrane is relatively easily pulverized and soluble in an organic solvent such as thiopropionic acid
  • the eggshell membrane is a protein that is inherently insoluble in water.
  • a water-soluble hydrolysate or other water-soluble materials of the eggshell membrane component containing a specific quantity of activated thiol group can be obtained after subjecting to an appropriate chemical treatment, enzyme treatment or the like.
  • the soluble eggshell membrane component can be obtained by sequentially or simultaneously conducting a reductive reaction to cleave a protein cross-linking disulfide bond and a hydrolytic reaction to partially cut a high molecular weight protein (see JP-A-2005-194663 for greater detail).
  • binders may be dissolved and mixed into the eggshell membrane solution if needed.
  • Commonly-known soluble polymers can be used as the binder, such as polyurethane, silicone, polyvinylidene fluoride, polyacrylonitrile, polymethyl methacrylate, polyvinyl chloride, polyvinylidene chloride, polyethylene, polypropylene, nylon, polyvinyl alcohol, cellulose, polyvinyl acetate, polyethylene oxide, polyethylene imide, polyaniline, polyethylene sulfide, polystyrene, polybutadiene, polyethylene terephthalate, polylactic resin, polyglutamic acid, hyaluronic acid and copolymers thereof.
  • binder examples include natural polymers such as starch, casein, collagen, nucleic acid, catechin, gelatin, sericin, fibroin, chitin, and chitosan, and sol solutions such as organosilica and organotitanium.
  • natural polymers such as starch, casein, collagen, nucleic acid, catechin, gelatin, sericin, fibroin, chitin, and chitosan
  • sol solutions such as organosilica and organotitanium.
  • the above-described polyphenols may be blended with the eggshell membrane solution to obtain the fiber that contains the eggshell membrane component and the polyphenols.
  • Water is usually used as a solvent, but other solvents that can dissolve the above-described components may be arbitrarily used.
  • the other solvents include acid, alkali, methanol, ethanol, propanol, isopropanol, acetone, ether, toluene, tetrahydrofuran, cyclohexane, N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, and chloroform.
  • One of the solvents that can disperse and dissolve the above-described binder uniformly in the eggshell membrane solution may be arbitrarily selected, but more than two kinds of the solvents that are mutually blended may be used in view of spinnability.
  • a cross-linking agent e.g., glutaraldehyde
  • surfactant metallic salt
  • thickner thickner
  • colorant preservative or various stabilizers
  • a blending quantity depends on a purpose such as strength of the finally obtained fiber and fiber assembly.
  • FIG. 1 is a perspective view and FIG. 2 is a cross sectional view showing an outline of the fiber producing device according to the exemplary embodiment of the present invention.
  • the electrospinning device is used as the fiber producing device, in which a voltage-applying roller 20 is provided in a solution immersion vessel 10 that is filled with the above-described eggshell membrane solution.
  • the voltage-applying roller 20 is rotatably supported in the solution immersion vessel 10 , where at least a circumference of the voltage-applying roller 20 is metallic.
  • a high-voltage generator 21 applies a voltage to the solution immersion vessel 10 which is connected to the high-voltage generator 21 . Accordingly, the eggshell membrane solution is positively charged and charges are concentrated on the voltage-applying roller 20 .
  • the voltage-applying roller 20 is connected to a rotary driving mechanism (not shown).
  • An accumulation conveyor 30 having an accumulation surface 30 A is provided to face the voltage-applying roller 20 , the accumulation surface 30 A facing the voltage-applying roller 20 .
  • a metallic block 31 is provided to face the voltage-applying roller 20 to interpose the accumulation conveyor 30 , and is connected to the high-voltage generator 21 to be negatively charged.
  • the accumulation conveyor 30 is a conductive material, for example, a belt-shaped member formed from aluminum or the like, or an unwoven fabric formed from a paper or a synthetic fiber that does not lower the conductivity of the metallic block 31 .
  • the accumulation conveyor 30 is unwound by an unwinding roller (not shown) and wound by a winding roller (not shown).
  • Two guide rollers 32 are arranged longitudinally in the flowing direction of the accumulation conveyor 30 of the metallic block 31 .
  • a distance between the voltage-applying roller 20 and the accumulation surface 30 A of the accumulation conveyor 31 is not particularly limited, and may be arbitrarily decided in accordance with a state of an accumulated fiber assembly so that a solvent is easily evaporated.
  • An applied voltage may vary depending on a property of the solution and an amount of the accumulated fiber assembly. The higher the voltage, the more fiber is obtained. For example, the voltage may be set in a range of 60 to 78 kV.
  • a static electric field is formed between the voltage-applying roller and the metallic block which work as two electrodes.
  • the eggshell membrane solution adhered on the voltage-applying roller is extracted in filaments toward the metallic block and accumulated on the accumulation surface.
  • the distance between the electrodes may be set in a range of 100 to 140 mm, for example.
  • the electrospinning method i.e., electrolytic spinning, electrostatic spinning
  • the fiber producing method using the electrospinning device shown in FIGS. 1 and 2 .
  • the accumulation conveyor 30 is driven. Since the metallic block 31 connected to the high-voltage generator 21 is provided near the accumulation conveyor 30 , the charges are induced and accumulated on a surface of the solution when the high voltage is applied. Thus generated electrostatic attractive force acts against a surface tension of the eggshell membrane solution. When an electric field force exceeds a critical value, the electrostatic attractive force exceeds the surface tension so that jets of the charged solution are ejected. Since the ejected jets have a large surface area relative to a volume, the solvent is effectively evaporated and a charge density increases due to decrease of the volume. Accordingly, the jets become finer. Due to the jet ejection of the solution, the solution is autonomously pulled toward the metallic block 31 for spinning.
  • the spun fiber containing the eggshell membrane component is accumulated on the accumulation surface 30 A of the accumulation conveyor 30 . Since the accumulation conveyor 30 is wound in a predetermined direction, the accumulated fiber (fiber assembly) containing the eggshell membrane component has a constant thickness along the longitudinal direction of the conveyor.
  • the voltage may be set in the range of 60 to 78 kV as described above.
  • the jets may not be generated when the voltage is too low and a spark may be generated when the voltage is too high.
  • the distance between the electrodes may be set in the range of 100 to 140 mm.
  • the solvent may not be sufficiently evaporated when the distance is too short and the jets may not be generated when the distance is too long.
  • a speed of the accumulation conveyor 30 may be arbitrarily set in a range of 4 to 10 cm per minute. The solvent may not be sufficiently evaporated when the speed is too slow and accumulation variations may be caused due to decrease of a Metsuke (a weight per unit area) when the speed is too fast.
  • a net Metsuke of the unwoven fabric that forms the accumulation conveyor 30 may be arbitrarily set in a range of 0.5 to 4.0 g per square meter by repeatedly accumulating the fiber under a condition that the accumulation variations are small. Under such condition, the fiber (fiber assembly) having a diameter in the range of 100 to 500 nm can be preferably produced.
  • the accumulated fiber assembly is exfoliated from the accumulation conveyor 30 by a particular device (not shown). Before or after the exfoliation, a conventional cross-linking process such as thermal cross-linking, ultraviolet cross-linking, radiation cross-linking, and glutaraldehyde cross-linking may be conducted.
  • a conventional cross-linking process such as thermal cross-linking, ultraviolet cross-linking, radiation cross-linking, and glutaraldehyde cross-linking may be conducted.
  • the electrospinning method is employed to obtain the ultrafine fiber without degrading the eggshell membrane component according to the exemplary embodiments, other fiber spinning methods may be employed as long as an object of the present invention can be achieved.
  • a plurality of static electric fields may be formed between a plurality of pairs of electrodes or in the solution immersion vessel.
  • a plurality of voltage-applying rollers 20 may have different voltage values.
  • the eggshell membrane solution was prepared and the fiber (fiber assembly) containing the eggshell membrane was produced by employing the electrospinning method as described below.
  • the electrospinning was performed using the above-described eggshell membrane solution under a condition that the voltage was 70 kV, the distance between electrodes was 100 mm and the speed of the accumulation conveyor 30 was 4 cm per minute in the electrospinning device shown in FIGS. 1 and 2 .
  • the accumulation conveyor 30 was formed from polypropylene (PP) unwoven fabric at which the eggshell membrane solution was repeatedly accumulated.
  • the charges were accumulated on the surface of the eggshell membrane solution. Then, the solution in filaments was jetted from the surface of the voltage-applying roller 20 when the charges increased over the surface tension of the solution. The jets of the solution were accumulated on the accumulation conveyor 30 while repeating evaporation of the solvent, increase of the charge density, and refinement of the filaceous solution.
  • the net Metsuke of the fiber assembly was 1.8 g per square meter.
  • FIGS. 5A and 5B show scanning electron micrographs of the fiber assembly.
  • the fiber diameter was about 460 nm. It should be understood from the scanning electron micrographs that the fiber assembly according to the present invention has the network structure imitating the eggshell membrane.
  • FIG. 6 shows a scanning electron micrograph of the above-described fiber assembly. It should be understood from the scanning electron micrograph that the fiber assembly according to the present invention has the network structure imitating the eggshell membrane.
  • the spinning was performed using the electrospinning device having the core/sheath nozzle as shown in FIGS. 4A and 4B under a condition that a supply ratio of the eggshell membrane solution: PEA aqueous solution was 1:3 to obtain a fiber assembly having a diameter of about 220 nm.
  • the fiber assembly was dipped in a methanol solution containing 2 mass % of iodine for two hours to conduct the oxidation and cross-linking process.
  • FIG. 7 shows a scanning electron micrograph of the fiber assembly before the oxidation and cross-linking process
  • FIG. 8 shows another scanning electron micrograph after the oxidation and cross-linking process. It should be understood from the scanning electron micrographs that the fiber assembly according to the present invention has the network structure imitating the eggshell membrane. Further, due to the oxidation and cross-linking process, an S—S bond that was dissociated when the eggshell membrane was dissolved was recombined and reconfigured in the insoluble material similar to the natural eggshell membrane.
  • the spinning was performed using the eggshell membrane solution obtained as described above in the electrospinning device as shown in FIG. 1 to obtain a fiber assembly.
  • the fiber assembly was dipped in the aqueous solution for 12 hours for an insolubilization process (chemical cross-linking) under a condition that glutaraldehyde/sodium sulfate/sulfuric acid was 0.06/0.96/0.4 (mol/l)
  • the fiber assembly was washed with water and dried at ambient temperature so that the fiber assembly having a fiber diameter of about 330 nm and containing the eggshell membrane component was obtained.
  • FIG. 9 shows a scanning electron micrograph of the above-described fiber assembly. It should be understood from the scanning electron micrograph that the fiber assembly according to the present invention has the network structure imitating the eggshell membrane.
  • EMP-TF water-solubilized eggshell membrane
  • FIG. 10 shows a scanning electron micrograph of the above-described fiber assembly. It should be understood from the scanning electron micrograph that the fiber assembly according to the present invention has the network structure imitating the eggshell membrane.
  • Example 2 After the eggshell membrane solution obtained in Example 1 was put on a glass petri dish and dried, the eggshell membrane solution was washed with methanol to obtain the film-shaped eggshell membrane.
  • the above-described eggshell membrane did not possess sufficient air permeability due to lack of the network structure of the natural eggshell membrane.
  • E-SF eggshell membrane power
  • the powder was too clingy to produce the fiber.
  • EMP-TF water-solubilized eggshell membrane
  • the eggshell membrane was too agglutinated to produce the fiber.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Manufacturing & Machinery (AREA)
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  • Dermatology (AREA)
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US20110177395A1 (en) * 2008-09-04 2011-07-21 Daiwabo Holdings Co., Ltd. Fiber assembly, composite of electro conductive substrate and fiber assembly, and production methods thereof
US20180185544A1 (en) * 2017-01-01 2018-07-05 Ying Shi Apparatus for treating human wound
US10590577B2 (en) 2016-08-02 2020-03-17 Fitesa Germany Gmbh System and process for preparing polylactic acid nonwoven fabrics
US10932952B2 (en) 2015-11-11 2021-03-02 Biovotec As Dry biocompatible disintegratable films for delivering particulate egg shell membrane to a wound
US11045578B2 (en) 2015-06-24 2021-06-29 Biovotec As Tissue engineering scaffolds comprising particulate egg shell membrane
TWI740165B (zh) * 2019-05-30 2021-09-21 鴻海精密工業股份有限公司 產生電能的裝置
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US20100297443A1 (en) * 2007-11-30 2010-11-25 Daiwabo Holdings Co., Ltd. Ultrafine composite fiber, ultrafine fiber, method for manufacturing same, and fiber structure
US20110177395A1 (en) * 2008-09-04 2011-07-21 Daiwabo Holdings Co., Ltd. Fiber assembly, composite of electro conductive substrate and fiber assembly, and production methods thereof
US8889573B2 (en) * 2008-09-04 2014-11-18 Daiwabo Holdings Co., Ltd. Fiber assembly, composite of electro conductive substrate and fiber assembly, and production methods thereof
US11992508B2 (en) 2014-10-28 2024-05-28 Biovotec As Micronized eggshell membrane particles and the use thereof to promote the healing of wounds
US12390495B2 (en) 2014-10-28 2025-08-19 Biovotec As Micronized eggshell membrane particles and the use thereof to promote the healing of wounds
US11045578B2 (en) 2015-06-24 2021-06-29 Biovotec As Tissue engineering scaffolds comprising particulate egg shell membrane
US10932952B2 (en) 2015-11-11 2021-03-02 Biovotec As Dry biocompatible disintegratable films for delivering particulate egg shell membrane to a wound
US10590577B2 (en) 2016-08-02 2020-03-17 Fitesa Germany Gmbh System and process for preparing polylactic acid nonwoven fabrics
US11441251B2 (en) 2016-08-16 2022-09-13 Fitesa Germany Gmbh Nonwoven fabrics comprising polylactic acid having improved strength and toughness
US20180185544A1 (en) * 2017-01-01 2018-07-05 Ying Shi Apparatus for treating human wound
TWI740165B (zh) * 2019-05-30 2021-09-21 鴻海精密工業股份有限公司 產生電能的裝置

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