US8747913B2 - Herbal extract pharmaceutical composition and method for treating and/or preventing of hyperlipidemia and processes for producing the same - Google Patents

Herbal extract pharmaceutical composition and method for treating and/or preventing of hyperlipidemia and processes for producing the same Download PDF

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US8747913B2
US8747913B2 US11/721,245 US72124505A US8747913B2 US 8747913 B2 US8747913 B2 US 8747913B2 US 72124505 A US72124505 A US 72124505A US 8747913 B2 US8747913 B2 US 8747913B2
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pharmaceutical composition
extract
parts
water extraction
extraction
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US20090291154A1 (en
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Xijun Yan
Boli Zhang
Zhixin Guo
Guoguang Zhu
Zhengliang Ye
Feng Wei
Ruizhi Luo
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Tasly Pharmaceutical Group Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734—Crataegus (hawthorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives

Definitions

  • the present invention relates to the pharmaceutical field. More specifically, the present invention relates to a pharmaceutical composition comprising Polygonum multiflorum Thunb, which provides the pharmacological effect of lowering blood lipid, their preparation method, their pharmacological effect and their use in preparation of the blood-lipid lowering drugs or the drugs for treatment and/or prevention of hyperlipidemia.
  • the composition of the present invention and its corresponding preparations can be used for treatment and/or prevention of hyperlipidemia.
  • the present invention also relates to the method of utilizing the composition for treatment and/or prevention of hyperlipidemia.
  • Polygonum multiflorum Thunb (Tuber fleeceflower root), also named as the Shouwu and Chi Shouwu in Chinese, originated from the dried root tuber of a perennial voluble herb plant of Polygonaceae Polygonum multiflorum Thunb, which belongs to a wild plant. It abounds in a lot of regions of China, mainly including Henan province, Hubei City, Guangxi City, Guangdong City, Guizhou province, Sichuan province and Jiangsu province, etc.
  • ZHANG et al. [ZHANG, Minfang et al, Clinically therapeutic study of Dispel Fatty Decoction in combination with sodium alginate diester in 35 cases of fatty liver, Shaanxi Journal of Traditional Chinese Medicine, 2002, Vol. 23, No. 10, 903-904] used the Dispel Fatty Decoction ( Salvia miltiorrhiza Bunge, Fructus Crataegi, Polygonum multiflorum Thunb, Semen Cassiae, Radix Curcumae and Rhizoma atractylodis etc.) in combination with sodium alginate diester to treat 35 cases of fatty liver, and a control group of 23 cases was set up. The results showed a significant difference between the therapeutic group and the control group. It was indicated that the integration of traditional Chinese medicine (TCM) and western medicine for treatment of fatty liver possessed many beneficial efficacies such as promoting lipid metabolism in liver, adjusting liver function and strengthening organic metabolism.
  • TCM traditional Chinese medicine
  • LI et al. [LI, Xiating et al, Clinically therapeutic analysis of Liver-Softening and Fat-Reducing Capsule in 45 cases of fatty liver, Jiangsu Journal of Traditional Chinese Medicine, 2000, Vol. 21, No. 6, 15-16] disclosed the application of the Liver-Softening and Fat-Reducing Capsule (which was prepared from Radix et Rhizoma Rhei Preparata, Radix Paeoniae Rubra, Salvia miltiorrhiza Bunge, crude Fructus Crataegi (Hawthorn fruit), Polygonum multiflorum Thunb and Concha Ostreae etc. for treating fatty liver.
  • the capsule can effectively improve liver functions and the level of blood lipid of fatty liver patients with the marked improvement in the images of liver.
  • Polygonum multiflorum Thunb has many functions such as nourishing liver and kidney, benefiting essence and blood and relaxing bowels.
  • Modern pharmacological studies show that many positive efficacies had been found for this herbal medicine, which was present not only in lowering cholesterol, decreasing cholesterol absorption through intestine and stopping its deposition in blood, relieving and alleviating the formation of arteriosclerosis, holding back the stagnation of lipids in blood or permeation into endarterium, but also improving the microcirculation and preventing the formation of thrombus.
  • Salvia miltiorrhiza Bunge and Radix Notoginseng are characterized in promoting blood circulation and removing blood stasis and improving microcirculation. By working with Fructus Crataegi, the purposes of improving the microcirculation and lowering blood lipid may be achieved simultaneously, and therefore will be beneficial to its therapeutic effects of hyperlipidemia in clinic.
  • the objective of the present invention is to provide a pharmaceutical composition
  • a pharmaceutical composition comprising Polygonum multiflorum Thunb.
  • Said composition and its preparations of all kind have a function of lowering blood lipid and can be used for treatment and/or prevention of hyperlipidemia and for controlling and/or alleviating various hyperlipidemia-related cardio and cerebral vascular diseases.
  • This composition comprises Polygonum multiflorum Thunb, Salvia miltiorrhiza Bunge, Fructus Crataegi and Radix Notoginseng.
  • the pharmaceutical composition may comprise the conventional pharmaceutically acceptable carriers.
  • All of the crude medicines used in this invention are in line with the standards of Chinese Pharmacopeia. They may be and preferably used as the form of the prepared medicinal herbs (“Yinpian” is referred to the herbs that are processed from the crude medicines).
  • composition of the present invention can be prepared into any of conventional preparations, in particular oral preparations.
  • the composition of the present invention comprises 10 to 20 parts by weight of Polygonum multiflorum Thunb, 5 to 15 parts by weight of Salvia miltiorrhiza Bunge, 10 to 20 parts by weight of Fructus Crataegi and 1 to 10 parts by weight of Radix Notoginseng.
  • composition of the present invention may also comprise one or more pharmaceutically acceptable carriers, wherein said carriers may be the conventional ones known in the pharmaceutical area.
  • said carriers are liquid or solid excipients, diluters, wetting agents, preservatives, sweeteners, flavoring agents as well as colorants etc.
  • the most conventional carriers are exemplified as starch, lactose, talc powder and/or dextrine etc.
  • the type and/or amount of the carriers are selected according to the knowledge known in the pharmaceutical area. Generally, the amount of the carriers varies greatly, for example, the carriers may account for 1 wt % to even several folds of the total amount of the crude medicines.
  • Another objective of the present invention is to provide a method for preparing said pharmaceutical composition.
  • Said composition can be prepared by a conventional method known in prior art.
  • the crude medicines of Polygonum multiflorum Thunb, Salvia miltiorrhiza Bunge, Fructus Crataegi and Radix Notoginseng are pulverized directly into powder, which is followed by preparing into the desired preparations.
  • the method of the present invention comprises the following steps:
  • Polygonum multiflorum Thunb, Salvia miltiorrhiza Bunge, Fructus Crataegi and Radix Notoginseng can be prepared into their extracts firstly, and then these extracts are prepared into the composition.
  • the preparation method for the composition of the present invention comprises the following steps:
  • an additional drying step at temperature of about 50 ⁇ 60° C., for example 55° C., for about 10 ⁇ 12 hours may be further included in the method.
  • each of four crude medicines can be extracted separately, or 2-4 kinds of these four crude medicines are mixed in random combination, such as the mixture of Polygonum multiflorum Thunb and Salvia miltiorrhiza Bunge, the mixture of Salvia miltiorrhiza Bunge and Radix Notoginseng, or the mixture of Polygonum multiflorum Thunb, Salvia miltiorrhiza Bunge and Radix Notoginseng, then the mixtures are extracted separately.
  • the aforesaid four crude medicines are extracted by conventional methods, for example, the methods are, but do not limited to, water extraction-alcohol precipitation method, percolate method, and column chromatography etc.
  • the extract process may be selected from water extraction method, alcohol extraction method, or the method of water extraction-alcohol precipitation followed by separation with macro-porous resin
  • the extract process may be selected from water extraction method, or the method of alcohol extraction followed by separation with macro-porous resin
  • the extract process may be selected from water extraction method, or the method of alcohol extraction followed by separation with macro-porous resin
  • Fructus Crataegi the extract process may be selected from water extraction method, or the method of water extraction followed by separation with polyamide resin
  • Radix Notoginseng the extract process may be selected from ethanol extraction method, or the method of alcohol extraction followed by separation with macro-porous resin.
  • Polygonum multiflorum Thunb was taken and pulverized, into which water was added and extracted for several times. The resulting extracts were combined and concentrated to give the Polygonum multiflorum Thunb extract.
  • Salvia miltiorrhiza Bunge was taken, pulverized and passed through 20-mesh sieve. Water was added to extract for two times. For the first time, the medicinal material was soaked in water ( ⁇ 9 ⁇ 10 folds) and extracted by heating for 1.5 hours, and for the second time, water ( ⁇ 5 ⁇ 7 folds) was added and extracted by heating for 1 hour. Resulting decoctions were combined, concentrated properly and added 95% ethanol to make the concentration of ethanol at 50 ⁇ 70%, then concentrated by recovering ethanol to give the Salvia miltiorrhiza Bunge extract.
  • Salvia miltiorrhiza Bunge was taken, pulverized and passed through 20-mesh sieve. The herb was extracted with 7 ⁇ 9-fold volume of 70% ethanol by heating reflux twice, one hour for each time. The extracts were combined and concentrated to give the Salvia miltiorrhiza Bunge extract.
  • the extraction method of the active fraction of Salvia miltiorrhiza Bunge (the method of water extraction-alcohol precipitation): the medicinal material of Salvia miltiorrhiza Bunge was taken, pulverized and passed through 20-mesh sieve and extracted with water or ethanol. The extract was concentrated and precipitated with ethanol. After recovering ethanol, the extract was dissolved with water and separated by macro-porous resin column. The column was washed with water to eliminate impurities, then to wash with ethanol until the active fraction was totally separated. The dried Salvia miltiorrhiza Bunge extract was obtained after recovering ethanol.
  • Fructus Crataegi was taken and extracted with water at suitable temperature for several times, the extracts was combined and concentrated to obtain the Fructus Crataegi extract.
  • Fructus Crataegi was taken, extracted with ethanol by refluxing for several times. The extracts were combined and concentrated properly, followed by being applied on polyamide resin column for further separation. The washing was collected and concentrated to give the Fructus Crataegi extract.
  • Radix Notoginseng was extracted with 10-fold volume of 10% ⁇ 30% ethanol for three times, refluxing by heating 8 hours. The extracts were combined and concentrated to recover ethanol to give the Radix Notoginseng extract.
  • the extraction method of the active fraction of Radix Notoginseng (the method of alcohol extraction-column chromatography): Radix Notoginseng was taken, pulverized properly and extracted with ethanol twice. After filtrated, the filtrate was combined and concentrated to certain volume by recovering ethanol under reduced pressure. A proper amount of water was added, followed by recovering ethanol until no smell of ethanol. The resulting extract was applied on pretreated macro-porous resin column, washed with water until washing had no color and then washed with 50% ethanol. The eluents were collected and concentrated to give the Radix Notoginseng extract.
  • composition of the present invention can be obtained and further prepared into any of desired specified preparations, in particular oral preparations, for example either the oral solid ones such as tablet, pill, granule or capsule etc, or oral liquid preparations such as syrup and oral liquid.
  • oral preparations for example either the oral solid ones such as tablet, pill, granule or capsule etc, or oral liquid preparations such as syrup and oral liquid.
  • the composition can be added with conventional carriers by a conventional method known in the prior art to give a desired preparation.
  • talc powder and dextrine may be used as the carriers in preparing of oral preparations with all kinds of crude medicine extracts.
  • the total amount of talc powder and dextrine accounts for 7 ⁇ 20 wt % of the total amount of the crude medicine extracts, for example, the amount of talc powder accounts for 1 ⁇ 5 wt % and dextrine is 6 ⁇ 15 wt % of total amount of the crude medicine extracts.
  • talc powder is 1 ⁇ 2 wt % and dextrine is 6 ⁇ 7%.
  • composition had the function of lowering blood lipid and can be used for treatment and/or prevention of hyperlipidemia.
  • Another objective of the present invention is to provide an application of the composition of the present invention in preparation of drugs for treatment of lowering blood lipid, as well as drugs for treatment and/or prevention of hyperlipidemia.
  • the pharmaceutical composition of the present invention may be used for treatment and/or prevention of hyperlipidemia, and for controlling and/or alleviating various hyperlipidemia-related cardio and cerebral vascular diseases.
  • Another objective of the present invention is to provide a method for treatment and/or prevention of hyperlipidemia, comprising administering a patient in need of this treatment with a therapeutically effective amount of the pharmaceutical composition and its corresponding preparations.
  • compositions and their preparations produced by any one of method of the present invention may be used for lowering blood lipid or treating and/or preventing hyperlipidemia.
  • daily orally-administrated amount of the composition for an adult patient corresponds to 10 ⁇ 50 g of the total amount of the crude medicine, preferably 20 ⁇ 30 g, once a day or several times such as two to three times. If they were used for prevention of disease, the dosage may be properly reduced.
  • FIG. 1 is the photograph of hepar tissue slice of the mice with hyperlipidemia (normal diet) in the control group after experiment.
  • FIG. 2 is the photograph of hepar tissue slice of the mice with hyperlipidemia (normal diet) in the therapeutic group after experiment.
  • FIG. 3 is the statistical analysis on hepatic steatosis of the mice with hyperlipidemia (normal diet) in the control group and the therapeutic group after experiment.
  • FIG. 4 is the photograph of hepar tissue slice of the mice with hyperlipidemia (high-fatty diet) in the control group after experiment.
  • FIG. 5 is the photograph of hepar tissue slice of the mice with hyperlipidemia (high-fatty diet) in the therapeutic group after experiment.
  • FIG. 6 is the statistical analysis on hepatic steatosis of the mice with hyperlipidemia (high-fatty diet) in the control group and the therapeutic group after experiment.
  • FIG. 7 is the photograph of renal tissue slice of the mice with hyperlipidemia (high-fatty diet) in the control group after experiment.
  • FIG. 8 is the photograph of renal tissue slice of the mice with hyperlipidemia (high-fatty diet) in the therapeutic group after experiment.
  • FIG. 9 is the statistical analysis on renal glomerulus lipid deposition of the mice with hyperlipidemia (high-fatty diet) in the control group and the therapeutic group after experiment.
  • FIG. 10 is the statistical analysis on aorta atherosclerosis of the mice with hyperlipidemia (high-fatty diet) in the control group and the therapeutic group after experiment, wherein said “HF” refers to high-fatty diet.
  • the objective of this test was to investigate the blood lipid lowering effect of the present pharmaceutical composition.
  • Drugs used in the test were the capsules prepared from the following Example 4 (hereinafter referred to as “HeShouWu Capsule”, or in short “HSWC”).
  • Inclusion criteria are healthy subjects with moderately elevated cholesterol levels. Exclusion criteria are those with underlying cardiovascular or other diseases or those in the treatment period of the cardiovascular diseases.
  • subjects were administrated with the HSWC or the corresponding amount of the placebo, wherein the HSWC were the capsules prepared from Example 4 of the present invention, and the dosage is 3 times/day and 3 capsules/time.
  • LDL Low-density lipoprotein
  • HDL High-density lipoprotein
  • TG triglyceride
  • Compliance of vascular system Measuring elasticity of arteries and calculating the change in volume associated with the change in blood pressure;
  • Flow mediated dilatation Using high-resolution ultrasonic apparatus of brachial artery to measure the change of vein in diameter with reactive hyperaemia;
  • Cutaneous vascular reactivity Using Laser Doppler Velocimetry with DC iontophoresis to measure cutaneous blood flux.
  • the objective of this test was to investigate the therapeutic effect of the present pharmaceutical composition on hyperlipidemia-associated diseases.
  • Drugs used in the test were the capsules prepared from Example 7.
  • mice Male Apo E knocked-out mice (this type of mouse is the animal model of primary hyperlipidemia) at 10 weeks of age, weighing 20 g, were selected and tested for 2 months, half of which were fed with normal diet (fat: 4.3%, cholesterol: 0.02%, the normal diet group) and the other with high-fatty diet (fat: 16.0%, cholesterol: 1.0%, the high-fatty diet group). Either the normal diet group or the high-fatty diet group was divided into the therapeutic group and the control group, ten for each group.
  • the therapeutic group was administered with the HSWC-dissolved drinking water (the concentration of the drug is about 0.8 g/L), so that the dosage of the drug for the normally-drinking mouse is about 90 ⁇ 160 mg drug/kg/d (expressed in the total amount of the crude medicines) for 2 months, and the control group with the HSWC-free drinking water.
  • all mice were executed and their liver, kidney and aortic vessel were taken out to make into tissue slice, and stained with oil-red O for the observation by microscope.
  • the amount of the crude medicines is 0.3 g in each capsule.
  • the amount of the crude medicines is 0.3 g in each capsule.
  • the amount of the crude medicines is 0.3 g in each capsule.
  • Polygonum multiflorum Thunb extract Polygonum multiflorum Thunb was weighed and extracted by percolating with 80% ethanol. Resulting extract was applied on a column of macroporous absorptive resin and eluted with 50% ethanol. The elutant was collected, and concentrated by recovering ethanol under reduced pressure and further heated to give 15 g of Polygonum multiflorum Thunb extract powder;
  • Fructus Crataegi extract Fructus Crataegi was weighed and extracted with 50% ethanol ( ⁇ 15 folds) twice, 60 minutes for each time. Resulting extracts were combined and concentrated by recovering ethanol, and further concentrated to give 15 g of the Fructus Crataegi extract powder.
  • the amount of the aforesaid extracts is 0.3 g in each capsule, which corresponds to 3 g of the crude medicines.
  • the capsules were prepared according to the process of Example 4, except that 0.9 g of talc powder and 3.15 g of dextrine were added, and the mixture was loaded into 150 capsules.
  • the amount of the aforesaid extracts is 0.3 g in each capsule, which corresponds to 3 g of the crude medicines.
  • the medicinal material of Salvia miltiorrhiza Bunge was pulverized and passed through 20-mesh sieve and extracted with water by heating twice: for the first time, the medicinal material was soaked in water ( ⁇ 9 ⁇ 10 folds) and extracted by heating for 1.5 hours, and for the second time, water ( ⁇ 5 ⁇ 7 folds) was added and extracted by heating for 1 hour. Resulting decoctions were combined, concentrated properly and 95% ethanol was added to make the concentration of ethanol at 50 ⁇ 70%, concentrated by recovering ethanol to give 6 g of the Salvia miltiorrhiza Bunge extract powder.
  • the amount of the aforesaid extracts is 0.3 g in each capsule, which corresponds to 3 g of the crude medicines.
  • the capsules were prepared according to the process of Example 4, except that 0.72 g of talc powder and 2.86 g of dextrine were added, and the mixture was loaded into 150 capsules.
  • the amount of the aforesaid extracts is 0.3 g in each capsule, which corresponds to 3 g of the crude medicines.
  • the amount of the aforesaid extracts is 0.3 g in each capsule, which corresponds to 3 g of the crude medicines.
  • the amount of the aforesaid extracts is 0.3 g in each tablet, which corresponds to 3 g of the crude medicines.

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CN200410093887 2004-12-10
CN200410093887XA CN1785284B (zh) 2004-12-10 2004-12-10 一种含有何首乌的药物组合物
CN200410093887.X 2004-12-10
PCT/CN2005/002089 WO2006060951A1 (fr) 2004-12-10 2005-12-05 Composition pharmaceutique pour le traitement et/ou la prevention de l’hyperlipidemie, procedes de production et utilisation de cette composition

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US20090304822A1 (en) 2008-06-09 2009-12-10 Chi-Hua Chen Extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum and compositions for improving metabolic syndrome
CA2756823C (fr) * 2009-03-30 2017-01-17 Tianjin Tasly Pharmaceutical Co., Ltd. Nouveau compose de l'acide l-salvianolique, methode pour sa preparation et son utilisation
US20120164242A1 (en) * 2010-12-27 2012-06-28 Wen-Jen Yu Novel methods for modulating melanin production
CN102614271A (zh) * 2012-04-20 2012-08-01 北京绿源求证科技发展有限责任公司 一种治疗糖尿病性血脂异常的中药
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CN103239525B (zh) * 2013-05-23 2014-11-05 山西省肿瘤研究所 一种活血降脂药酒及其制备方法
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CN1785284A (zh) 2006-06-14
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WO2006060951A1 (fr) 2006-06-15
AU2005313755B2 (en) 2008-07-31
US20090291154A1 (en) 2009-11-26
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