WO1998010102A3 - Bacteries multiresistantes, procedes d'obtention et utilisation - Google Patents

Bacteries multiresistantes, procedes d'obtention et utilisation Download PDF

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Publication number
WO1998010102A3
WO1998010102A3 PCT/FR1997/001566 FR9701566W WO9810102A3 WO 1998010102 A3 WO1998010102 A3 WO 1998010102A3 FR 9701566 W FR9701566 W FR 9701566W WO 9810102 A3 WO9810102 A3 WO 9810102A3
Authority
WO
WIPO (PCT)
Prior art keywords
glnp
genes
gene
psts
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1997/001566
Other languages
English (en)
Other versions
WO1998010102A2 (fr
Inventor
Patrick Ludovic-Henri Duwat
Alexandra Dorothy Gruss
Emmanuelle Ghislaine Je Maguin
Fabien Claude Rallu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9610926A external-priority patent/FR2753201B1/fr
Application filed by Institut National de la Recherche Agronomique INRA filed Critical Institut National de la Recherche Agronomique INRA
Priority to EP97938986A priority Critical patent/EP0941308A2/fr
Priority to AU41228/97A priority patent/AU4122897A/en
Priority to CA002266190A priority patent/CA2266190A1/fr
Publication of WO1998010102A2 publication Critical patent/WO1998010102A2/fr
Publication of WO1998010102A3 publication Critical patent/WO1998010102A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne des bactéries présentant une résistance aux stress améliorée par rapport à la souche sauvage, caractérisées en ce qu'elles comportent au moins une mutation dans un gène qui altère l'activité normale dudit gène, ce gène étant impliqué dans le transport d'électron, d'acides aminés, d'oligopeptides, du phosphate, de la réparation de l'ADN, de la stabilité des ARN ou de nouveaux gènes et choisi parmi: les gènes de la voie de biosynthèse des GP, les gènes du clone R1.1 (pstS), R1.4 (arl1), R1.5 (glnP), R1.7 (carB), R1.8 (glnP), R1.14 (arl2), R1.16 (glnP), R1.17 (glnQ), R1.20 (arl3), R2.6 (pstB), R2.9 (recN), R2.11 (arl4), R2.15 (arl5), R2.17 (arl7), R2.20 (arl8, yybT), pstS, pnpA, et trl1.
PCT/FR1997/001566 1996-09-06 1997-09-05 Bacteries multiresistantes, procedes d'obtention et utilisation Ceased WO1998010102A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97938986A EP0941308A2 (fr) 1996-09-06 1997-09-05 Bacteries multiresistantes, procedes d'obtention et utilisation
AU41228/97A AU4122897A (en) 1996-09-06 1997-09-05 Multi-resistant bacteria, methods for obtaining them and their uses
CA002266190A CA2266190A1 (fr) 1996-09-06 1997-09-05 Bacteries multiresistantes, procedes d'obtention et utilisation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9610926A FR2753201B1 (fr) 1996-09-06 1996-09-06 Bacteries multiresistantes, procedes d'obtention et utilisation
FR96/10926 1996-09-06
FR9611555A FR2753202A1 (fr) 1996-09-06 1996-09-23 Bacteries multiresistantes, procedes d'obtention et utilisation
FR96/11555 1996-09-23

Publications (2)

Publication Number Publication Date
WO1998010102A2 WO1998010102A2 (fr) 1998-03-12
WO1998010102A3 true WO1998010102A3 (fr) 1998-05-14

Family

ID=26232947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1997/001566 Ceased WO1998010102A2 (fr) 1996-09-06 1997-09-05 Bacteries multiresistantes, procedes d'obtention et utilisation

Country Status (5)

Country Link
EP (1) EP0941308A2 (fr)
AU (1) AU4122897A (fr)
CA (1) CA2266190A1 (fr)
FR (1) FR2753202A1 (fr)
WO (1) WO1998010102A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
CA2605162A1 (fr) * 2005-04-15 2006-10-26 North Carolina State University Procedes et compositions pour moduler l'adhesion et la resistance au stress chez des bacteries
CN109628364B (zh) * 2019-01-03 2020-12-29 江南大学 一种提高乳酸菌对酸性条件的耐受能力的方法

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; ZHAO G ET AL: "An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B-6) as well as the aromatic amino acids and vitamins for growth.", XP002051188 *
H. HALL ET AL: "Molecular responses of microbes to environmental pH stress", ADVANCES IN MICROBIAL PHYSIOLOGY, vol. 37, 1995, pages 229 - 272, XP000674366 *
HARTKE A ET AL: "UV-inducible proteins and UV-induced cross-protection against acid, ethanol, H-2O-2 or heat treatment in Lactococcus lactis subsp. lactis.", ARCHIVES OF MICROBIOLOGY 163 (5). 1995. 329-336. ISSN: 0302-8933, XP000672629 *
J. FOSTER AND H. HALL: "Inducible pH homeostasis and acid tolerance response of Salmonella typhimurium", JOURNAL OF BACTERIOLOGY, vol. 173, no. 16, August 1991 (1991-08-01), pages 5129 - 5135, XP000674345 *
JOURNAL OF BACTERIOLOGY 176 (19). 1994. 6134-6138. ISSN: 0021-9193 *
MAGUIN E ET AL: "Efficient insertional mutagenesis in lactococci and other gram-positive bacteria.", JOURNAL OF BACTERIOLOGY 178 (3). 1996. 931-935. ISSN: 0021-9193, XP000673892 *
NILSSON D ET AL: "ISOLATION OF PURINE AUXOTROPHIC MUTANTS OF LACTOCOCCUS -LACTIS AND CHARACTERIZATION OF THE GENE HPT ENCODING HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE.", MOL GEN GENET 235 (2-3). 1992. 359-364. CODEN: MGGEAE ISSN: 0026-8925, XP002032525 *
RALLU F ET AL: "Lactococcus lactis and stress.", ANTONIE VAN LEEUWENHOEK 70 (2-4). 1996. 243-251. ISSN: 0003-6072, October 1996 (1996-10-01), XP000673964 *

Also Published As

Publication number Publication date
WO1998010102A2 (fr) 1998-03-12
FR2753202A1 (fr) 1998-03-13
CA2266190A1 (fr) 1998-03-12
AU4122897A (en) 1998-03-26
EP0941308A2 (fr) 1999-09-15

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