WO1999047536A3 - Verfahren zur synthese von nucleinsäuremolekülen - Google Patents

Verfahren zur synthese von nucleinsäuremolekülen Download PDF

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Publication number
WO1999047536A3
WO1999047536A3 PCT/DE1999/000755 DE9900755W WO9947536A3 WO 1999047536 A3 WO1999047536 A3 WO 1999047536A3 DE 9900755 W DE9900755 W DE 9900755W WO 9947536 A3 WO9947536 A3 WO 9947536A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
conducting
synthesis
coupled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1999/000755
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English (en)
French (fr)
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WO1999047536A2 (de
Inventor
Annette Bernauer
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Individual
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Individual
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Application filed by Individual filed Critical Individual
Priority to AU36998/99A priority Critical patent/AU3699899A/en
Priority to EP99919096A priority patent/EP1047706A2/de
Publication of WO1999047536A2 publication Critical patent/WO1999047536A2/de
Publication of WO1999047536A3 publication Critical patent/WO1999047536A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Synthese von Nucleinsäuremolekülen. Insbesondere betrifft die Erfindung ein derartiges Verfahren, das rekursiv durchgeführt wird. Die Nucleinsäurekomponenten sind vorzugsweise synthetischen oder semisynthetischen Ursprungs. Das Prinzip des erfindungsgemässen Verfahrens beruht darauf, dass an bzw. mit einem bereitgestellten Nucleinsäuremolekül ein weiteres Nucleinsäuremolekül angelagert und/oder verknüpft wird, das Ende des bereitgestellten Nucleinsäuremoleküls maskiert wird, falls an dieses bzw. mit diesem kein weiteres Nucleinsäuremolekül angelagert und/oder verknüpft wurde, das weitere Nucleinsäuremolekül an einer vorbestimmten Stelle gespalten wird, wobei vorzugsweise wiederum ein Ende entsteht, an das bzw. mit dem ein weiteres Nucleinsäuremolekül angelagert und/oder verknüpft werden kann, und die vorgenannten Verfahrensschritte gegebenenfalls so oft wiederholt werden, bis das gewünschte Produkt synthetisiert ist. Die Erfindung betrifft ferner einen Kit zur Durchführung des erfindungsgemässen Verfahrens.
PCT/DE1999/000755 1998-03-19 1999-03-18 Verfahren zur synthese von nucleinsäuremolekülen Ceased WO1999047536A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU36998/99A AU3699899A (en) 1998-03-19 1999-03-18 Method for conducting the synthesis of nucleic acid molecules
EP99919096A EP1047706A2 (de) 1998-03-19 1999-03-18 Verfahren zur synthese von nucleinsäuremolekülen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998112103 DE19812103A1 (de) 1998-03-19 1998-03-19 Verfahren zur Synthese von Nucleinsäuremolekülen
DE19812103.2 1998-03-19

Publications (2)

Publication Number Publication Date
WO1999047536A2 WO1999047536A2 (de) 1999-09-23
WO1999047536A3 true WO1999047536A3 (de) 2000-01-27

Family

ID=7861560

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000755 Ceased WO1999047536A2 (de) 1998-03-19 1999-03-18 Verfahren zur synthese von nucleinsäuremolekülen

Country Status (4)

Country Link
EP (1) EP1047706A2 (de)
AU (1) AU3699899A (de)
DE (1) DE19812103A1 (de)
WO (1) WO1999047536A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19925862A1 (de) * 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
WO2001036679A2 (en) * 1999-11-15 2001-05-25 Hartwell John G METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
DE10145553B4 (de) * 2001-09-16 2006-06-08 Gene Architects Ag Nukleinsäure für einen Klonierungsvektor
EP1314783B1 (de) * 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
JP4663988B2 (ja) * 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
ES2310197T3 (es) 2002-10-18 2009-01-01 Sloning Biotechnology Gmbh Metodo para la preparacion de moleculas de acido nucleico.
ES2353302T3 (es) 2004-01-23 2011-03-01 Sloning Biotechnology Gmbh Producción enzimática de novo de moléculas de ácido nucleico.
EP2028272B1 (de) * 2006-06-06 2014-01-08 Panasonic Corporation Verfahren zur modifizierung einer nukleotidkette
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
EP3257942B1 (de) 2008-12-18 2020-07-22 ITI Scotland Limited Verfahren zum zusammensetzen von polynukleinsäuresequenzen
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP6214530B2 (ja) 2011-07-15 2017-10-18 ザ ジェネラル ホスピタル コーポレイション 転写活性化因子様エフェクターの組立て方法
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9890364B2 (en) 2012-05-29 2018-02-13 The General Hospital Corporation TAL-Tet1 fusion proteins and methods of use thereof
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
EP3789405A1 (de) 2012-10-12 2021-03-10 The General Hospital Corporation Transkriptionsaktivator-like-effektor-lysinspezifische demethylase-1 (lsd1)-fusionsproteine
EP2954042B1 (de) 2013-02-07 2017-12-06 The General Hospital Corporation Tale-transkriptionsaktivatoren
DE102013006487A1 (de) 2013-04-10 2014-11-06 ATG: biosynthetics GmbH Iterative Assemblierung und Deassemblierung von vorbestimmten, artifiziellen DNA -Konstrukten und dem spezifischen Austausch von funktionalen genetischen Elementen
SG11202012762WA (en) * 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
JP7587425B2 (ja) 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
US20240384319A1 (en) * 2021-09-17 2024-11-21 The University Of Manchester Synthesis of Oligonucleotides

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497272A1 (de) * 1991-01-31 1992-08-05 Becton, Dickinson and Company Strangverdrängungsamplifikation
WO1993019202A2 (en) * 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Exchangeable template reaction
US5466586A (en) * 1989-05-10 1995-11-14 Akzo Nobel N.V. Method for the synthesis of ribonucleic acid (RNA)
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
DE19633427A1 (de) * 1996-08-20 1998-03-19 Hubert S Dr Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466586A (en) * 1989-05-10 1995-11-14 Akzo Nobel N.V. Method for the synthesis of ribonucleic acid (RNA)
EP0497272A1 (de) * 1991-01-31 1992-08-05 Becton, Dickinson and Company Strangverdrängungsamplifikation
WO1993019202A2 (en) * 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Exchangeable template reaction
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
DE19633427A1 (de) * 1996-08-20 1998-03-19 Hubert S Dr Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J.FUJIMOTO ET AL.: "Synthesis and Cleavage of Oligodeoxynucleotides Containing a 5-Hydroxyuracil Residue at a Defined Site.", CHEMICAL RESEARCH IN TOXICOLOGY, vol. 10, no. 11, November 1997 (1997-11-01), pages 1254 - 1258, XP000856548 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
AU3699899A (en) 1999-10-11
WO1999047536A2 (de) 1999-09-23
DE19812103A1 (de) 1999-09-23
EP1047706A2 (de) 2000-11-02

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