WO2000077220A3 - Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können - Google Patents

Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können Download PDF

Info

Publication number
WO2000077220A3
WO2000077220A3 PCT/DE2000/001950 DE0001950W WO0077220A3 WO 2000077220 A3 WO2000077220 A3 WO 2000077220A3 DE 0001950 W DE0001950 W DE 0001950W WO 0077220 A3 WO0077220 A3 WO 0077220A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acids
substrate amino
methylate
pps
activation domains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2000/001950
Other languages
English (en)
French (fr)
Other versions
WO2000077220A2 (de
Inventor
Ullrich Keller
Florian Schauwecker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU59651/00A priority Critical patent/AU5965100A/en
Priority to DE10081623T priority patent/DE10081623D2/de
Priority to CA002418798A priority patent/CA2418798A1/en
Priority to EP00945641A priority patent/EP1190066A2/de
Publication of WO2000077220A2 publication Critical patent/WO2000077220A2/de
Publication of WO2000077220A3 publication Critical patent/WO2000077220A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die Erfindung betrifft die Veränderung von Peptidsynthetasen (PPS) in der Weise, daß sie ihre Substrataminosäuren N-methylieren können. PPS sind Enzyme, die Peptide auf nicht-ribosomale Weise synthetisieren. Die PPS sind modular aufgebaut. Jedes Modul besitzt eine Aktivierungsdomäne, welche die jeweilige Substrataminosäure erkennt und kovalent bindet. Die von der PPS katalysierte Peptidsynthese erfolgt dann durch die Kondensation der kovalent gebundenen Substrataminosäuren. Eine geringe Anzahl der bekannten Aktivierungsdomänen ist in der Lage, die gebundenen Substrataminosäuren auch zu N-methylieren. Die hier beschriebene Erfindung ermöglicht die Umwandlung von Aktivierungsdomänen ohne N-Methyltransferase-Aktivität in Aktivienngsolomänen nu7 N-île-thyltransferase-Aktivität, wobei die ursprüngliche Substratspezifität erhalten bleibt.
PCT/DE2000/001950 1999-06-16 2000-06-15 Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können Ceased WO2000077220A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU59651/00A AU5965100A (en) 1999-06-16 2000-06-15 Method of modifying peptide synthetases such that they can n-methylate their substrate amino acids
DE10081623T DE10081623D2 (de) 1999-06-16 2000-06-15 Verfahren zur Veränderung von Peptidsynthetasen in der Weise, daß sie ihre Substrataminosören N-methylieren können
CA002418798A CA2418798A1 (en) 1999-06-16 2000-06-15 Method of modifying peptide synthetases such that they can n-methylate their substrate amino acids
EP00945641A EP1190066A2 (de) 1999-06-16 2000-06-15 Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19928313.3 1999-06-16
DE1999128313 DE19928313A1 (de) 1999-06-16 1999-06-16 Verfahren zur Veränderung von Peptidsynthefasen in der Weise, daß sie ihre Substrataminosäuren N-methylieren können

Publications (2)

Publication Number Publication Date
WO2000077220A2 WO2000077220A2 (de) 2000-12-21
WO2000077220A3 true WO2000077220A3 (de) 2001-03-29

Family

ID=7911976

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/001950 Ceased WO2000077220A2 (de) 1999-06-16 2000-06-15 Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können

Country Status (5)

Country Link
EP (1) EP1190066A2 (de)
AU (1) AU5965100A (de)
CA (2) CA2418798A1 (de)
DE (2) DE19928313A1 (de)
WO (1) WO2000077220A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11180738B2 (en) 2016-04-07 2021-11-23 Eth Zurich Method for producing an n-methylated (poly) peptide

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0578616A2 (de) * 1992-07-09 1994-01-12 Sandoz Ltd. Zyclosporine Synthetase
EP0637630A2 (de) * 1993-07-13 1995-02-08 ENIRICERCHE S.p.A. Verfahren zur biologischen Herstellung von Peptiden
EP0789078A2 (de) * 1995-08-09 1997-08-13 ENIRICERCHE S.p.A. Modifizierte Peptidsynthetasen, und deren Verwendung in die nicht-ribosomaler Herstellung von Peptiden
WO1999002659A2 (en) * 1997-07-09 1999-01-21 Biochemie Gesellschaft M.B.H. Method of altering the domains of cyclosporin synthetase to give a modified cyclosporin synthetase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0578616A2 (de) * 1992-07-09 1994-01-12 Sandoz Ltd. Zyclosporine Synthetase
EP0637630A2 (de) * 1993-07-13 1995-02-08 ENIRICERCHE S.p.A. Verfahren zur biologischen Herstellung von Peptiden
EP0789078A2 (de) * 1995-08-09 1997-08-13 ENIRICERCHE S.p.A. Modifizierte Peptidsynthetasen, und deren Verwendung in die nicht-ribosomaler Herstellung von Peptiden
WO1999002659A2 (en) * 1997-07-09 1999-01-21 Biochemie Gesellschaft M.B.H. Method of altering the domains of cyclosporin synthetase to give a modified cyclosporin synthetase

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BILLICH A ET AL.: "Formation of N-methylated peptide bonds in peptides and peptidols", BIOCHEM. PEPT. ANTIBIOT. (EDITOR(S): KLEINKAUF H; VON DOEHREN H.) PUBL: DE GRUYTER, BERLIN, FED. REP. GER., 1990, pages 57 - 59, XP000965466 *
BURMESTER J ET AL: "Highly conserved N- methyltransferases as an integral part of peptide synthetases.", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 37, no. 2, October 1995 (1995-10-01), pages 201 - 207, XP000965322 *
KELLER U: "Actinomycin synthetases. Multifunctional enzymes responsible for the synthesis of the peptide chains of actinomycin.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 262, no. 12, 25 April 1987 (1987-04-25), pages 5852 - 5856, XP002153253 *
MARAHIEL M A ET AL: "Modular peptide synthetases involved in nonribosomal peptide synthesis", CHEMICAL REVIEWS, vol. 97, no. 7, November 1997 (1997-11-01), pages 2651 - 2673, XP002133489 *
SCHAUWECKER F ET AL.: "Construction and in vitro analysis of a new bi-modular polypeptide synthetase for synthesis of N-methylated acyl peptides", CHEMISTRY AND BIOLOGY, vol. 7, no. 4, 20 March 2000 (2000-03-20), pages 287 - 297, XP000957461 *
SCHAUWECKER F ET AL.: "Molecular cloning of the actinomycin synthetase gene cluster from Streptomyces chrysomallus and functional heterologous expression of the gene encoding actinomycin synthetase II", JOURNAL OF BACTERIOLOGY, vol. 180, no. 9, May 1998 (1998-05-01), pages 2468 - 2474, XP002153252 *
SCHNEIDER A ET AL: "Targeted alteration of the substrate specificity of peptide synthetases by rational module swapping", MOLECULAR AND GENERAL GENETICS, vol. 257, no. 3, February 1998 (1998-02-01), pages 308 - 318, XP002141778 *

Also Published As

Publication number Publication date
WO2000077220A2 (de) 2000-12-21
AU5965100A (en) 2001-01-02
EP1190066A2 (de) 2002-03-27
DE10081623D2 (de) 2002-05-29
DE19928313A1 (de) 2000-12-21
CA2377592A1 (en) 2000-12-21
CA2418798A1 (en) 2002-11-05

Similar Documents

Publication Publication Date Title
EP2385103A3 (de) Abweichender Hypocrea jecorina CBH1
JPH06504758A (ja) プロテアーゼ耐性を有する修飾ペプチドのライブラリー
AU1541497A (en) Nucleic acid molecules from plants coding enzymes which participate in the starch synthesis
PL345092A1 (en) Nucleic acid molecules which code for enzymes derived from wheat and which are involved in the synthesis of starch
EP2368907A3 (de) Anti-Abeta-Antikörper und ihre Verwendung
DE69623005D1 (de) Verbesserte verfahren zur enzymatischen synthese von oligosacchariden
WO1997013845A3 (en) Phosphopantetheinyl transferases and uses thereof
WO2002020565A3 (en) Collections of repeat proteins comprising repeat modules
WO2000073501A3 (de) Nucleinsäuremoleküle mit spezifischer erkennung von nativem prpsc, herstellung und verwendung
WO2004058946A3 (en) Protein arrays
ATE354668T1 (de) Verfahren zur herstellung von tripeptiden
WO2003008638A3 (en) Sorting and immobilization system for nucleic acids using synthetic bidning systems
Hamilton et al. Artificial receptors for biologically active molecules
WO1997007219A3 (en) Genes and proteins controlling cholesterol synthesis
US9976133B2 (en) Synzymes
EP2017343A3 (de) Protein-A-basierte Bindungsdomäne mit gewünschten Aktivitäten
WO2000012732A3 (en) Organelle targeting sequences
WO2000077220A3 (de) Verfahren zur veränderung von peptidsynthetasen in der weise, dass sie ihre substrataminosäuren n-methylieren können
WO1999055718A3 (en) Solution phase biopolymer synthesis
MXPA02004829A (es) Metodo para la sintesis estereoselectiva de aminoacidos ciclicos.
EP1336603A4 (de) Verfahren zur einführung von aminogruppen und verfahren zur synthese von aminosäuren
Karakama et al. One-pot synthesis of 2, 5-diketopiperazine with high titer and versatility using adenylation enzyme
WO2001041811A3 (en) Carrier peptide and method for delivery of molecules into cells
Cao et al. Macrolactonization catalyzed by the terminal thioesterase domain of the nonribosomal peptide synthetase responsible for lichenysin biosynthesis
WO2003089641A3 (en) Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000945641

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 59651/00

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2000945641

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10018113

Country of ref document: US

REF Corresponds to

Ref document number: 10081623

Country of ref document: DE

Date of ref document: 20020529

WWE Wipo information: entry into national phase

Ref document number: 10081623

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 2418798

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000945641

Country of ref document: EP