WO2002013613A1 - Method for producing working reagent and for biological prevention or control - Google Patents

Method for producing working reagent and for biological prevention or control Download PDF

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Publication number
WO2002013613A1
WO2002013613A1 PCT/CN2000/000237 CN0000237W WO0213613A1 WO 2002013613 A1 WO2002013613 A1 WO 2002013613A1 CN 0000237 W CN0000237 W CN 0000237W WO 0213613 A1 WO0213613 A1 WO 0213613A1
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Prior art keywords
agent
microbial
substance
plant
styling
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PCT/CN2000/000237
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English (en)
French (fr)
Inventor
Shihpanyu Hsieh
Ruizhi Huang
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Yuen Foong Yu Paper Mfg Co Ltd
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Yuen Foong Yu Paper Mfg Co Ltd
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Priority to PCT/CN2000/000237 priority Critical patent/WO2002013613A1/zh
Priority to AU2000266812A priority patent/AU2000266812A1/en
Priority to CNB008198209A priority patent/CN100473281C/zh
Priority to EP00954262A priority patent/EP1312263A4/en
Priority to JP2002518767A priority patent/JP2004505995A/ja
Priority to MYPI20004672A priority patent/MY138985A/en
Publication of WO2002013613A1 publication Critical patent/WO2002013613A1/zh
Anticipated expiration legal-status Critical
Priority to US11/376,006 priority patent/US20060159660A1/en
Priority to US11/376,261 priority patent/US20060177425A1/en
Priority to US11/376,392 priority patent/US20060159661A1/en
Priority to US11/595,102 priority patent/US20070053882A1/en
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom

Definitions

  • the present invention relates to a method for manufacturing a microbial agent and its application in biological control to prevent the opportunity of biological infection by disease. Background of the invention
  • pesticides have been used in the past to control plant diseases and insect pests in order to increase crop yield and reduce the probability of crop diseases.
  • the pesticide itself is a chemical agent. If the application concentration is too high and it is close to the late harvest, it will be caused by the improper use of the method, which will cause the toxicity of pesticide residues in the crops, endangering people's health and causing the pesticide to accumulate Phenomenon of body or acute poisoning.
  • microorganisms to control plant diseases in a non-pesticidal way to prevent plant diseases.
  • Using microorganisms to prevent plant diseases can eliminate the problem of pesticide residues on crops and maintain people's health. It can eliminate the problem that the large amount of pesticides harm the environment.
  • the control microorganism is applied to the crop cultivation area by spraying, but this method cannot surely send the control microorganism to the location where the disease source invades.
  • the location of the pathogen invasion is most common on leaf sheaths.
  • the spraying method is used to control the disease and insect pests, the movement of the sprayer ’s lateral resistance spray rod will cause rice to be produced on the plant.
  • due to the difficulty of the movement of the spray rod a large number of wounds were found on the rice plant body. Instead of effectively acting on the leaf sheath, it increased the chance of other pathogens invading the injured rice and increased the chance of disease.
  • the present invention is based on careful testing and research and a persevering spirit, and finally created the present invention "a manufacturing method of an agent and its application in biological control" to solve the above application. Missing and hurting. The following is a brief description of this case.
  • the purpose of the present invention is to provide a microbial agent instead of the use of traditional pesticides, and solve the problems of abuse and improper use of a large number of pesticides.
  • Another object of the present invention is to provide a microbial agent and its application in biological control, which can correctly enter the path position of a plant caused by a pathogen to enhance the effect of disease control and killing the pathogen.
  • Another object of the present invention is to provide a microbial agent and a method for applying the same to biological control, so that the microbial agent acts on the affected plant body to perform anti-biotic effect, super-parasitic effect and competitive action. It is used to inhibit the activity of pathogens and achieve the purpose of controlling diseases.
  • the main purpose of the present invention is to provide a method for manufacturing a microbial agent, the manufacturing method includes providing a microbial agent, which can act on an object, and secondly, the microbial agent and a modeling substance are combined into an intermediate. Finally, a styling agent is added to pre-shape to form the agent.
  • the agent further comprises a carrier for carrying the microbial agent.
  • the microbial agent is an antagonistic bacteria, which is Gliocladium virens, which is used to antagonize Rhizoctonia solani AG-l.
  • the modeling material contains a binder, a culture solution, and a floating agent.
  • the binding substance is sodium alginate (Sodium Alginate :) for modeling purposes;
  • the culture fluid is a thick spore culture fluid for cultivating the microorganism;
  • the floating agent may be a non-water-friendly substance.
  • the substance or air is to provide the agent with a floating property, wherein the non-water-compatible substance is a vegetable salad oil of soybean salad oil.
  • the styling agent is a solution of calcium chloride (CaCl 3 ), and is used to produce a coacervation with one of the binding substances in the styling substance, wherein the binding substance is sodium alginate.
  • CaCl 3 calcium chloride
  • Yet another important object of the present invention is to provide a method for acting with an agent, the method comprising providing an agent having a microbial agent, and contacting the agent with an object, so that the agent The agent releases the microbial agent to the object.
  • the way that the agent contacts the object is to make the agent adsorb on the object-.
  • Yet another important object of the present invention is to provide a method for controlling plant diseases with an agent.
  • the method includes providing an agent, and the agent has a microbial agent, which can be used to control a plant disease. After the agent comes into contact with a plant body, the agent releases the microbial agent on the plant body to control plant diseases.
  • the method is applicable to a plant hydroponic system.
  • the present invention provides an optimal method for manufacturing an agent.
  • the method is as follows: firstly, a microbial effector is provided to act on an object; the microbial effector is combined with a modeling substance to form an intermediate, and finally an The styling agent is preformed to form the agent.
  • the detailed steps are described below.
  • a carrier is first provided for carrying the microbial agent.
  • a modeling substance is added, and the modeling substance contains a binder, a culture solution, and a floating agent.
  • the binder used here is 2% Sodium Alginate, which can coacervate with calcium chloride for modeling purposes.
  • the culture medium used is a thick spore culture medium.
  • the floating agent can use a substance that is not compatible with water or inject air.
  • the agent has a floating property. In the present invention, 10% of a substance that is not compatible with water is used.
  • a vegetable-based soy salad oil that acts as a buoyant.
  • the floating microbial agent can be used as a microbial agent in the above-mentioned agents.
  • Buoyancy is a characteristic of the water surface of a hydroponic plant system, and biological control experiments are performed based on it. Because of the surface tension of water and the flow of water, the floating microbial agent will approach the rice stalk and tightly adhere to the rice leaf sheath, thereby occupying the leaf sheath growth space and living on the leaf sheath.
  • antibiotic effect, superparasite effect and competition effect were carried out to inhibit the pathogenic activity and achieve the disease control effect. The implementation process and results are described below.
  • the floating microbial agent of Gliocladium virens was applied to each area in the first experimental area when the rice tillering period was about 50 days.
  • the application amount was 100 grams, and another 50 grams was applied every other week.
  • a plot without any control measures was added as a control test group and a thick spore culture solution of Gliocladium virens microorganism was applied once and three times to operate.
  • Each of the above tests was performed in quadruplicate, and the paper mat change and the incidence of rice leaf blight were recorded during the growth of rice. The fresh weight and dry seeds of rice were recorded at the time of harvest, and the rice blight was measured.
  • the relative height of the diseased spot on the rice diseased plant, and the relative height of the diseased spot is calculated by the following formula: Relative height of the diseased spot o / _ Highest point visible lesion (Highest Point a Lesion Is Seen) cm lQQ a
  • the test results are shown in Table 1.
  • Table 1 the relative lesion height of the floating microbial agent of Gliocladium virens was 13.4%, and the relative height of the lesion was 3 times when the thick spore culture solution of the Gliocladium virens microorganism was applied three times.
  • % The relative lesion height of a single application of the thick spore culture solution of the Gliocladium virens microorganism was 37.6%.
  • the results of these three tests were compared with the control test group of the plot without any control measures, and the relative lesion height was 41.4%.
  • test results are shown in Table 2.
  • Table 2 the relative lesion height of the floating microbial agent of Gliocladium virens was 18.75%, and the relative lesion height of the thick spore culture solution of the Gliocladium virens microorganism three times was 13.5%.
  • the results of these three tests were 47.25% relative to the control spot of the control group without any control measures. Afterwards, all showed obvious differences, showing that the rice with the floating microbial agent of Gliocladium virens, had a relatively reduced height of diseased spots and had a control effect. test results
  • Table 1 Floatability of Gliocladimn virens in the first test area
  • Microbial agents as a result of rice biological control.
  • Microbial agents as a result of rice biological control.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Description

一种作用剂制造方法及其生物防治之应用 发明领域
本发明系关于一种微生物作用剂之制造方法及其于生物防治的应用， 以防治 生物受病害感染之机会。 发明背景
一般在对于防治植物病虫害， 以往皆以使用农药来进行防治植物病虫害， 以 提高作物产量及降低作物罹患病害的机率。但农药本身是一种化学制剂，若施用的 浓度过高及接近釆收后期才施用，此因使用的方法不当，会使作物产生农药残留的 毒性问题， 而危及人们的健康， 造成农药积聚于人们体内或急性中毒现象。
而现阶段在以非农药方式防治植物病害上， 以一种微生物来进行生物性防 - 治， 已成为可能， 利用微生物来防治植物病害， 可以解除农药残留于作物之问题以 维护人们健康， 亦可消除大量使用农药危害环境的问题。
一般在施用生物防治方法时， 是利用喷洒方式将该防治性微生物施用于作物 栽培区，但此种方式并不能确实将该防治性微生物确切的送至病源侵入的位置。以 水稻枯紋病为例，病原侵入的位置最常见于叶鞘上，若以喷洒方式来操作防治病虫 害的过程，会因为实施喷洒的水稻叶横阻喷杆的移动，造成水稻于植物体上产生伤 口， 同时因喷杆的移动不易， 更造成大量伤口处见于水稻植物体上， 非但不能有效 作用于叶鞘上，反而增加其他病原菌侵入该受伤水稻的机会，使发生病害的机会提 高。
因此， 本发明鉴于习之技术之缺失， 乃悉心试验与研究并一本锲而不舍之精 神， 终创作出本发明 "一种作用剂之制造方法及其于生物防治之应用"， 以解决上 述之施用缺失与伤害。 以下为本案之简要说明。
'
发明简要说明
本发明之目的在于提供一种微生物作用剂以代替传统农药之使用， 解决大量 农药被滥用与使用不当之问题。
本发明之另一目的在于提供一种微生物作用剂及其应用于生物防治， 能正确 . 的进入病原造成植物病害的途径位置， 以提升病害防治与杀死病原的效果。
本发明之又一目的为提供一微生物作用剂及其应用于生物防治之方法， 使该 微生物性作用剂作用于该受作用之植物体， 以进行抗生作用、超寄生作用与竞争作 用等用于抑制病原之活性， 达到防治病害之目的。
是以本发明之主要目的， 在提供一种微生物作用剂之制造方法， 其制造法包 含提供一种微生物作用物，其可作用于一物体，其次使此微生物作用物与一造型物 质结合成一中间物， 最后加入一造型剂， 俾予造型而形成该作用剂。
根据上述构想， 其中该作用剂更包含一载体， 用以携带该微生物作用物。 而 其中之微生物作用物， 为一种拮抗菌， 系为黏帚霉菌 Gliocladium virens ， 用以拮 抗水稻紋枯病原菌 (Rhizoctonia solani)AG-l。
根据上述构想， 其中该造型物质含有一粘合物、 一培养液与一漂浮剂。 该粘 合物为藻酸钠 (Sodium Alginate：)，供以作造型之用；该培养液为一种厚孢子培养液， 用以培养该微生物作用物；该漂浮剂可以是一不与水亲和之物质或是空气， 以提供 该作用剂具有一漂浮性质，其中该不与水亲和之物质为一种植物性沙拉油之大豆沙 拉油。
根据上述构想， 其中该造型剂为氯化钙 (CaCl3)溶液， 用以与该造型物质中之 一粘合物产生凝聚作用， 其中该粘合物为藻酸钠 (Sodium Alginate), 为供以作造型 之用。
本发明之再另一重要目的为提供一种以作用剂进行作用之方法， 此方法包含 提供一作用剂， 该作用剂具有一微生物作用物， 并使该作用剂与一物体接触， 从而 使该作用剂释出该微生物作用物于该物体。
根据上述构想， 该作用剂与该物体接触之方式为使该作用剂吸附于该物体 - 上。
本发明之再另一重要目的为提供一种以作用剂防治植物病害之方法， 方法包 含提供一作用剂， 且该作用剂具有一微生物作用物， 其可用以防治一种植物病害， 经使该作用剂与一植物体接触后， 使该作用剂释出该微生物作用物于该植物体上， 以进行防治植物病害。
根据上述构想， 其中该方法适用于一植物水耕系统。
由上所述， 本发明得藉由下面之实施例中之详细说明， 俾得一更深入了解。 实施例说明
本发明提供一种作用剂之最佳制造方法， 其方法为： 首先提供一种微生物作 用物， 用于作用于一物体； 再使该微生物作用物与一造型物质结合成一中间物， 最 后加入一造型剂， 俾予造型而形成该作用剂。 其详细步骤说明如下所述。
首先提供以一载体， 用以携带该微生物作用物。 在本发明中是以一种黏帚霉 菌， 其学名为 Gliocladium virens作为一拮抗菌， 来作为上述作用剂中之微生物作 用物， 用以拮抗水稻紋枯病原菌， 其学名为 Rhizoctonia solani AG-l , 以作为防治 水稻枯紋病之用。
接著， 再添加入一造型物质， 此造型物质含有一粘合物、 一培养液与一漂浮 剂。其中所使用的粘合物为 2 % 的藻酸钠 (Sodium Alginate), 可与氯化钙产生凝聚 作用，供以作造型之用， 所使用之培养液为一种厚孢子培养液， 是用来培养微生物 作用物质， 即本发明所述之 Gliocladium virens 黏帚霉菌。 而漂浮剂， 可使用一不 与水亲和之物质或是以打入空气的方式，来提供该作用剂具有一漂浮性质，在本发 明中是使用 10 %的一不与水亲和之物质之植物性大豆沙拉油， 作为漂浮剂。
最后步骤加入一氯化钙 (CaCl2)溶液作为造型剂， 与上述之藻酸钠 (Sodium
Alginate),产生凝聚作用，经干燥后，即完成此具有黏帚霉菌 Gliodadimn virens 之 漂浮性微生物作用剂制造过程。
在有关于应用此含有黏帚霉菌 Gliocladium virens 之拮抗菌， 作为上述作用剂 中之微生物作用物， 以防治拮抗水稻紋枯病之生物防治实验，乃利用此具漂浮性微 生物作用剂，可藉由浮力漂浮一水耕植物系统水面上的特性，而据以实施生物防治 试验。 因为水的表面张力及水的流动§区使此漂浮性微生物作用剂会向水稻稻秆靠 近， 而紧紧吸附在水稻叶鞘上， 藉以占据叶鞘生长空间， 并存活于叶鞘上， 在病原 靠近时， 随即进行抗生作用、超寄生作用与竞争作用来抑制病原活性，达到病害防 治功效。 其实施过程与结果说明如下。
在第一试验区， 是以水稻插秧后， 在水稻分蘖盛期约在第 50天时， 均勾施用 黏帚霉菌 Gliocladium virens 之漂浮性微生物作用剂于第一试验区中的每一小区， 每小区施用量为 100克， 隔周每小区再施用 50克。 如上述方式， 并加上一无任何 防治措施之小区以作为对照试验组及一只施用黏帚霉菌 Gliocladium virens 微生物 之厚孢子培养液 1次与 3次之试验来操作。 以上之试验， 各施以四重复试验， 并在 在水稻生长期间纪录纸席变化及水稻枯紋病发生机率，且于收割时纪录水稻之鲜重 与干种，及测量罹患水稻枯纹病之水稻病株上病斑的相对病斑高度，其相对病斑高 度， 是以下列式子计算而得： 相对病斑高度 o/ _可见病斑最高点（Highest P oint a Lesion Is Seen)cm lQQ a
(Relative Lesion Height) °"： 水稻植物体高度 (KantHei gl^cm " ° 在第二试验区， 是以水稻插秧后， 在水稻生长第 70天时， 均勾施用黏帚霉菌 Gliocladium virens 之漂浮性微生物作用剂于第一试验区中的每一小区， 每小区施 用量为 100克， 第 77天每小区再施用 100克， 第 84天每小区再施用 50克。 如上 述方式， 并加上一无任何防治措施之小区以作为对照试验组及一只施用黏帚霉菌 Gliocladium virens 微生物之厚孢子培养液 1次与 3次之试验来操作。以上之试验， 各施以四重复试验。并测量罹患水稻枯纹病之水稻病株上病斑的相对病斑高度，其 相对病斑高度， 是以下列式子计算而得：
相对病斑高度 %_可见病斑最高点（Highest P oint a Lesion Is Seen)cm :, 1QQ
(Relative Lesion Height) 水稻植物体高度（Plant Hei ght)cm °
在第一试验区， 其试验结果详见表一。 由表一中可知， 经施用黏帚霉菌 Gliocladium virens 之漂浮性微生物作用剂的相对病斑高度为 13.4 %， 施用黏帚霉 菌 Gliocladium virens微生物之厚抱子培养液 3次的相对病斑高度为 23.4 %， 施用 黏帚霉菌 Gliocladium virens 微生物之厚孢子培养液 1 次的相对病斑高度为 37.6 %， 此三试验结果经与无任何防治措施之小区的对照试验组， 其相对病斑高度为 41.4 %比较后， 在统计上皆呈现明显的差异， 显示该施用黏帚霉菌 Gliocladium virens 之漂浮性微生物作用剂的水稻， 其相对病斑高度降低， 具有防治效果。另外 经前述三种施用黏帚霉菌 Gliopladium virens G-8之漂浮性微生物作用剂、 施用黏 帚霉菌 Gliocladium virens G-8 微生物之厚孢子培养液 3 次与施用黏帚霉菌 Gliocladium virens G-8微生物之厚孢子培养液 1次处理之稻谷鲜重分别为 8148.8 公斤 /公顷、 7917.3公斤 / 公顷与 7593.2公斤 /公顷重， 亦与无任何防治措施之对 照组的 5898.7 公斤 /公顷的产量比较后， 呈现明显差异。 相同的， 前述三种处理 之稻谷干童分别为 6852.4公斤 / 公顷、 6620.9公斤 / 公顷与 6250.5公斤 /公顷重， 亦与对照组之 5648公斤 /公顷呈现明显差异。
在第二试验区，试验结果详见表二。由表二可见，经施用黏帚霉菌 Gliocladium virens 之漂浮性微生物作用剂的相对病斑高度为 18.75 %， 施用黏帚霉菌 Gliocladium virens 微生物之厚孢子培养液 3次的相对病斑高度为 13.5 %， 施用黏 帚霉菌 Gliocladium virens微生物之厚孢子培养液 1次的相对病斑高度为 29.5 %， 此三试验结果与无任何防治措施之小区的对照试验组， 其相对病斑高度为 47.25 %， 经比较后， 皆呈现明显的差异， 显示该施用黏帚霉菌 Gliocladium virens 之漂 浮性微生物作用剂的水稻， 其相对病斑高度降低， 具有防治效果。 试验结果
表一： 于第一试验区施用黏帚霉菌 Gliocladimn virens 之漂浮性
微生物作用剂作为水稻生物防治的结果。
Figure imgf000006_0001
(附注： 以上， 表格一之同一栏内之各项数值， 经 Duncan's多重范围分析检测 过， 在 p=0.05下， 并无显著差异 (Significantly Different)存在， 其数值足可个别代 表其四重复实验值。 ） 表二： 于第二试验区施用黏帚霉菌 Gliocladium virens 之漂浮性
微生物作用剂作为水稻生物防治的结果。
Figure imgf000006_0002
(附注： 以上， 表格一之同一栏内之各项数值， 经 Duncan's多重范围分析检测 过， 在 p=0.05下， 并无显著差异 (Significantly Different)存在， 其数值足可个别代 表其四重复实验值。 ） 综上所述， 可知本发明所揭露之应用此一微生物作用剂制造方法而得的产品 于生物防治上， 相对于习知技术， 确实能达到防治植物病害， 降低水稻植物体受病 原菌感染之病班相对高度，且可广泛应用于欲各类欲制造该作用剂之方法及其应用 于生物防治方面，充分达成本发明之目的。本发明得由熟悉此技艺之人士任施匠思 而为诸般修饰， 然皆不脱如附申请专利范围所欲作用者。

Claims

权 利 要 求
1.一种作用剂制造方法， 包含以下步骤：
(1)提供一种微生物作用物， 该微生物作用物可作用于一物体；
(2)使该微生物作用物与一造型物质结合成一中间物；
(3)加入一造型剂， 俾予该中间物造型而形成该作用剂。
2.如权利要求 1所述的方法， 其中该作用剂更包含一载体，用以携带该微生物 作用物。
3.如权利要求 1所述的方法， 其中该微生物作用物， 系为一种拮抗菌。
4.如权利要求 3所述的方法，其中该拮抗菌，系为黏帚霉菌 Gliocladium virens。
5.如权利要求 4 所述的方法， 其中该拮抗菌系用以拮抗水稻紋枯病原菌 (Rhizoctonia solani)AG-l。
6.如权利要求 1所述的方法， 其中该造型物质含有一粘合物、一培养液与一漂 浮剂。
7.如权利要求 6所述的方法， 其中该粘合物为藻酸钠 (Sodium Alginate), 供以 作造型之用。
8.如权利要求 6所述的方法，其中该培养液为一种厚孢子培养液， 用以培养该 微生物作用物。
9.如权利要求 6所述的方法， 其中该漂浮剂， 系选自于一不与水亲和之物质与 空气其中之一， 以提供该作用剂具有一漂浮性质。
10.如权利要求 9 所述的方法， 其中该不与水亲和之物质为一种植物性沙拉 , 油， 系为一大豆沙拉油。
11.如权利要求 1所述的方法， 其中该造型剂为氯化钙溶液， 用以与该造型物 质中之一粘合物产生凝聚作用。
12.如权利要求 11所述的方法， 其中该粘合物为藻酸钠 (Sodium Alginate), 供 以作造型之用。
13.—种以作用剂进行作用之方法， 包含:
(1)提供一作用剂， 该作用剂具有一微生物作用物；
(2)使该作用剂与一物体接触；
(3)使该作用剂释出该微生物作用物于该物体。
14.如权利要求 13所述的方法， 其中该作用剂更包含一载体， 用以携带该微生 物作用物。
15.如权利要求 13所述的方法， 其中该微生物作用物， 系为一种拮抗菌。
16.如权利要求 15 所述的方法， 其中该拮抗菌， 系为黏帚霉菌 Gliocladimn virens■>
17.如权利要求 16 所述的方法， 其中该拮抗菌系用以拮抗水稻紋枯病原菌 (Rhizoctonia s'olani) AG-1。
18.如权利要求 13所述的方法，该作用剂与该物体接触之方式为使该作用剂吸 附于该物体上。
19.一种以作用剂防治楦物病害之方法， 包含-
(1)提供一作用剂， 该作用剂具有一微生物作用物， 其可用以防治一种植物 病害；
(2)使该作用剂与一植物体接触；
(3)使该作用剂释出该微生物作用物于该植物体， 以进行防治植物病害。
20.如权利要求 19所述的方法， 其中该方法适用于一植物水耕系统。
21.如权利要求 19所述的方法， 其中该作用剂更包含一载体，用以携带该微生 物作用物。
22.如权利要求 19所述的方法， 其中该微生物作用物， 系为一种拮抗菌。
23.如权利要求 22 所述的方法， 其中该拮抗菌， 系为黏帚霉菌 Gliocladium virens 。
24.如权利要求 23 所述的方法， 其中该拮抗菌系用以拮抗水稻紋枯病原菌 (Rhizoctonia solani)AG- 1。
25.如权利要求 19所述的方法，该作用剂与该物体接触之方式为使该作用剂吸 附于该物体上。
PCT/CN2000/000237 2000-08-16 2000-08-16 Method for producing working reagent and for biological prevention or control Ceased WO2002013613A1 (en)

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AU2000266812A AU2000266812A1 (en) 2000-08-16 2000-08-16 Method for producing working reagent and for biological prevention or control
CNB008198209A CN100473281C (zh) 2000-08-16 2000-08-16 一种漂浮性制剂制造方法及其生物防治之应用
EP00954262A EP1312263A4 (en) 2000-08-16 2000-08-16 PROCESS FOR THE PREPARATION OF A REAGENT, ITS THERAPEUTIC AND PROPHYLACTIC BIOLOGICAL APPLICATIONS
JP2002518767A JP2004505995A (ja) 2000-08-16 2000-08-16 微生物作用剤の製造方法及び生物病害防除方法
MYPI20004672A MY138985A (en) 2000-08-16 2000-10-06 Method for producing microorganism agent and its biocontrol application
US11/376,006 US20060159660A1 (en) 2000-08-16 2006-03-15 Method for producing working reagent and for biological prevention or control
US11/376,261 US20060177425A1 (en) 2000-08-16 2006-03-15 Method for producing working reagent and for biological prevention or control
US11/376,392 US20060159661A1 (en) 2000-08-16 2006-03-15 Method for producing working reagent and for biological prevention or control
US11/595,102 US20070053882A1 (en) 2000-08-16 2006-11-09 Method for producing microorganism agent

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668512A (en) * 1985-03-20 1987-05-26 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi and nutrient for control of soilborne plant pathogens
US4724147A (en) * 1983-06-22 1988-02-09 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi for control of soilborne diseases

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4718935A (en) * 1983-06-22 1988-01-12 The United States Of America As Represented By The Secretary Of Agriculture Method for the preparation of mycoherbicide-containing pellets
US4818530A (en) * 1983-06-22 1989-04-04 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi for control of soilborne diseases
ATE69263T1 (de) * 1984-09-21 1991-11-15 Cernitin Sa Lebende mikroorganismen enthaltendes fluessiges praeparat, verfahren zu dessen herstellung, lebende mikroorganismen enthaltendes pelletiertes produkt und verfahren zu dessen herstellung.
DE69130327D1 (de) * 1990-12-07 1998-11-12 Univ Temple Stabilisierte, insektizide, nematode-enthaltende mittel
KR0145740B1 (ko) * 1991-05-23 1998-08-01 채영복 고정화 미생물 농약과 그의 제조방법
US5358863A (en) * 1993-03-30 1994-10-25 The United States Of America As Represented By The Secretary Of The Agriculture Oil and absorbent coated granules containing encapsulated living organisms for controlling agricultural pests
JPH10182309A (ja) * 1996-12-25 1998-07-07 Japan Tobacco Inc 水面浮遊性微生物農薬

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4724147A (en) * 1983-06-22 1988-02-09 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi for control of soilborne diseases
US4668512A (en) * 1985-03-20 1987-05-26 The United States Of America As Represented By The Secretary Of Agriculture Preparation of pellets containing fungi and nutrient for control of soilborne plant pathogens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1312263A4 *

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