WO2002061064A3 - Verfahren zur herstellung von rekombinantem trypsin - Google Patents

Verfahren zur herstellung von rekombinantem trypsin Download PDF

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Publication number
WO2002061064A3
WO2002061064A3 PCT/EP2002/001072 EP0201072W WO02061064A3 WO 2002061064 A3 WO2002061064 A3 WO 2002061064A3 EP 0201072 W EP0201072 W EP 0201072W WO 02061064 A3 WO02061064 A3 WO 02061064A3
Authority
WO
WIPO (PCT)
Prior art keywords
trypsin
producing recombinant
recombinant trypsin
trypsinogen
autolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/001072
Other languages
English (en)
French (fr)
Other versions
WO2002061064A2 (de
Inventor
Rainer Mueller
Stephan Glaser
Frank Geipel
Johann-Peter Thalhofer
Bernhard Rexer
Claus Schneider
Michael Ratka
Stephanie Ronning
Hellmut Eckstein
Claudia Giessel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8176376&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002061064(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP02716713A priority Critical patent/EP1399568B1/de
Priority to JP2002561621A priority patent/JP4159878B2/ja
Priority to CA2437342A priority patent/CA2437342C/en
Priority to US10/470,508 priority patent/US7276605B2/en
Priority to AT02716713T priority patent/ATE310092T1/de
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to DE50204952T priority patent/DE50204952D1/de
Priority to CN02805619.1A priority patent/CN1545553B/zh
Publication of WO2002061064A2 publication Critical patent/WO2002061064A2/de
Anticipated expiration legal-status Critical
Publication of WO2002061064A3 publication Critical patent/WO2002061064A3/de
Priority to US11/853,483 priority patent/US7666629B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die vorliegende ERfindung beschreibt ein Verfahren zur Herstellung von rekombinanten Trypsin aus Schweinepankreas in Pichia pastoris löslich und sekretiert in das Kulturmedium, wobei duch Expression bei pH 3,0 - 4,0 die Aktivierung von Trypsinogen zu β-Trypsin und die Autolyse von β-Trypsin über α-Trypsin zu ε-Trypsin und von da aus zu inaktiven Peptiden weigtehend verhindert wird.
PCT/EP2002/001072 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin Ceased WO2002061064A2 (de)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CN02805619.1A CN1545553B (zh) 2001-02-01 2002-02-01 生产重组胰蛋白酶的方法
JP2002561621A JP4159878B2 (ja) 2001-02-01 2002-02-01 組換えトリプシンの製造方法
CA2437342A CA2437342C (en) 2001-02-01 2002-02-01 Method for producing recombinant trypsin
US10/470,508 US7276605B2 (en) 2001-02-01 2002-02-01 Method for producing recombinant trypsin
AT02716713T ATE310092T1 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin
EP02716713A EP1399568B1 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin
DE50204952T DE50204952D1 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin
US11/853,483 US7666629B2 (en) 2001-02-01 2007-09-11 Method for producing recombinant trypsin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01102342.1 2001-02-01
EP01102342 2001-02-01

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10470508 A-371-Of-International 2002-02-01
US11/853,483 Division US7666629B2 (en) 2001-02-01 2007-09-11 Method for producing recombinant trypsin

Publications (2)

Publication Number Publication Date
WO2002061064A2 WO2002061064A2 (de) 2002-08-08
WO2002061064A3 true WO2002061064A3 (de) 2003-12-24

Family

ID=8176376

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/001072 Ceased WO2002061064A2 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Country Status (10)

Country Link
US (2) US7276605B2 (de)
EP (1) EP1399568B1 (de)
JP (1) JP4159878B2 (de)
CN (1) CN1545553B (de)
AT (1) ATE310092T1 (de)
CA (1) CA2437342C (de)
CZ (1) CZ303658B6 (de)
DE (1) DE50204952D1 (de)
DK (1) DK1399568T3 (de)
WO (1) WO2002061064A2 (de)

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WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin
ATE439432T1 (de) * 2002-08-30 2009-08-15 Novozymes Inc Verfahren zur herstellung von säugetier-trypsinen
MY146082A (en) * 2005-09-14 2012-06-29 Sanofi Aventis Deutschland Cleavage of precursors of insulins by a variant of trypsin
WO2010079039A1 (en) * 2009-01-06 2010-07-15 Nestec S.A. Processing of macronutrients
CN101967469B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组胰蛋白酶的生产及应用
EP2475771B1 (de) * 2009-09-10 2019-06-05 Biocon Limited Neue prolipase-bovin-trypsinogenfusionsproteine
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103805584B (zh) * 2012-11-12 2016-08-24 宜昌东阳光长江药业股份有限公司 一种重组胰蛋白酶纯化方法
CN103405756B (zh) * 2013-07-10 2014-12-24 浙江众益制药股份有限公司 药物组合物复方消化酶胶囊(ii)及其制备方法
MA39835A (fr) 2014-04-17 2017-02-22 Biogen Ma Inc Compositions et procédés de modulation de l'épissage du smn2 chez un patient
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
WO2016040748A1 (en) 2014-09-12 2016-03-17 Ionis Pharmaceuticals, Inc. Compositions and methods for detection of smn protein in a subject and treatment of a subject
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
US10851371B2 (en) 2015-04-10 2020-12-01 Ionis Pharmaceuticals, Inc. Modulation of SMN expression
WO2017218884A1 (en) 2016-06-16 2017-12-21 Ionis Pharmaceuticals, Inc. Combinations for the modulation of smn expression
CN106350497B (zh) * 2016-11-24 2019-07-16 天津大学 一种胰蛋白酶及其制备方法与应用
CN110511916B (zh) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 一种重组胰蛋白酶的生产工艺
PH12022552258A1 (en) 2020-02-28 2023-11-20 Ionis Pharmaceuticals Inc Compounds and methods for modulating smn2
CN115216463B (zh) * 2022-06-15 2023-08-15 武汉瀚海新酶生物科技有限公司 具有稳定性的重组胰蛋白酶及其制备方法和应用
KR20250110226A (ko) 2022-11-23 2025-07-18 에프. 호프만-라 로슈 아게 재조합 단백질 발현을 증가시키는 방법

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Publication number Priority date Publication date Assignee Title
EP0597681A1 (de) * 1992-11-13 1994-05-18 Eli Lilly And Company Expressionsvektore für Rinder-Trypsine und Trypsinogene und damit transformierte Wirtzellen
EP0699687A2 (de) * 1994-08-31 1996-03-06 The Green Cross Corporation Verfahren zur Reinigung von menschlichem Serumalbumin
WO1997000316A1 (en) * 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
WO2000017332A1 (en) * 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
WO2001055429A2 (en) * 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin

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EP0597681A1 (de) * 1992-11-13 1994-05-18 Eli Lilly And Company Expressionsvektore für Rinder-Trypsine und Trypsinogene und damit transformierte Wirtzellen
EP0699687A2 (de) * 1994-08-31 1996-03-06 The Green Cross Corporation Verfahren zur Reinigung von menschlichem Serumalbumin
WO1997000316A1 (en) * 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
WO2000017332A1 (en) * 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
WO2001055429A2 (en) * 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin

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Also Published As

Publication number Publication date
EP1399568A2 (de) 2004-03-24
DK1399568T3 (da) 2006-03-27
CA2437342A1 (en) 2002-08-08
EP1399568B1 (de) 2005-11-16
CZ303658B6 (cs) 2013-02-06
US7276605B2 (en) 2007-10-02
JP4159878B2 (ja) 2008-10-01
DE50204952D1 (de) 2005-12-22
CN1545553A (zh) 2004-11-10
CA2437342C (en) 2013-08-06
WO2002061064A2 (de) 2002-08-08
ATE310092T1 (de) 2005-12-15
CZ20032337A3 (cs) 2004-04-14
US20040203095A1 (en) 2004-10-14
CN1545553B (zh) 2011-09-21
JP2004520049A (ja) 2004-07-08
US7666629B2 (en) 2010-02-23
US20080064084A1 (en) 2008-03-13

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