WO2003102232A3 - Vermehrung von ribonukleinsäuren - Google Patents

Vermehrung von ribonukleinsäuren Download PDF

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Publication number
WO2003102232A3
WO2003102232A3 PCT/EP2003/005579 EP0305579W WO03102232A3 WO 2003102232 A3 WO2003102232 A3 WO 2003102232A3 EP 0305579 W EP0305579 W EP 0305579W WO 03102232 A3 WO03102232 A3 WO 03102232A3
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WIPO (PCT)
Prior art keywords
rna
sequence
triphosphates
dna
produced
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Ceased
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PCT/EP2003/005579
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English (en)
French (fr)
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WO2003102232A2 (de
Inventor
Peter Scheinert
Guido Krupp
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ARTUS - GESELLSCHAFT fur MOLEKULARBIOLOGISCHE DIAGNOSTIK und ENTWICKLUNG MBH
ARTUS GES fur MOLEKULARBIOLOG
Original Assignee
ARTUS - GESELLSCHAFT fur MOLEKULARBIOLOGISCHE DIAGNOSTIK und ENTWICKLUNG MBH
ARTUS GES fur MOLEKULARBIOLOG
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Publication date
Application filed by ARTUS - GESELLSCHAFT fur MOLEKULARBIOLOGISCHE DIAGNOSTIK und ENTWICKLUNG MBH, ARTUS GES fur MOLEKULARBIOLOG filed Critical ARTUS - GESELLSCHAFT fur MOLEKULARBIOLOGISCHE DIAGNOSTIK und ENTWICKLUNG MBH
Priority to CA2487538A priority Critical patent/CA2487538C/en
Priority to JP2004510468A priority patent/JP4372682B2/ja
Priority to EP03755950A priority patent/EP1509623B1/de
Priority to DE50311731T priority patent/DE50311731D1/de
Priority to AT03755950T priority patent/ATE437238T1/de
Priority to AU2003232835A priority patent/AU2003232835B2/en
Priority to US10/516,007 priority patent/US20060035226A1/en
Publication of WO2003102232A2 publication Critical patent/WO2003102232A2/de
Publication of WO2003102232A3 publication Critical patent/WO2003102232A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft Verfahren zur Vermehrung von Ribonukleinsäuren, die Schritte umfassen, bei denen man (a) mittels eines Einzelstrang-Primers definierter Sequenz, einer RNA-abhängigen DNA Polymerase und Desoxyribonukleosidtriphosphaten einen DNA-Einzelstrang durch reverse Transkription aus einer RNA erzeugt; (b) die RNA entfernt; (c) mittels eines Einzelstrang-Primers, der eine Box-Sequenz umfasst, einer DNA-Polymerase und Desoxyribonukleosidtriphosphaten einen DNA-Doppelstrang erzeugt; (d) den Doppelstrang in Einzelstränge auftrennt; (e) mittels eines Einzelstrang-Primers, der im 5'-Bereich die Sequenz eines Promotors und im 3'-Bereich dieselbe definierte Sequenz wie der Primer in (a) umfasst, einer DNA-Polymerase und Desoxyribonukleosidtriphosphaten DNA-Doppelstränge aus einem der in (d) entstandenen Einzelsträngen erzeugt; (f) mittels einer RNA-Polymerase und Ribonukleosidtriphosphaten eine Vielzahl von RNA Einzelsträngen erzeugt, die an beiden Enden definierte Sequenzen umfassen. Die Erfindung betrifft ferner Kits zur Vermehrung von Ribonukleinsäuren gemäss einem der obigen Verfahren, welche die folgenden Bestandteile umfassen: mindestens einen Einzelstrang-Primer, der die Sequenz eines Promotors umfasst; (b) mindestens einen Einzelstrang-Primer, der eine Box-Sequenz umfasst; (c) eine RNA-abhängige DNA-Polymerase; (d) Desoxyribonukleosidtriphosphate; (e) eine DNA-abhängige DNA-Polymerase; eine RNA-Polymerase; und (g) Ribonukleosidtriphosphate.
PCT/EP2003/005579 2002-05-31 2003-05-27 Vermehrung von ribonukleinsäuren Ceased WO2003102232A2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2487538A CA2487538C (en) 2002-05-31 2003-05-27 Amplification of ribonucleic acids
JP2004510468A JP4372682B2 (ja) 2002-05-31 2003-05-27 リボ核酸の増幅方法
EP03755950A EP1509623B1 (de) 2002-05-31 2003-05-27 Vermehrung von ribonukleinsäuren
DE50311731T DE50311731D1 (de) 2002-05-31 2003-05-27 Vermehrung von ribonukleinsäuren
AT03755950T ATE437238T1 (de) 2002-05-31 2003-05-27 Vermehrung von ribonukleinsäuren
AU2003232835A AU2003232835B2 (en) 2002-05-31 2003-05-27 Amplification of ribonucleic acids
US10/516,007 US20060035226A1 (en) 2002-05-31 2003-05-27 Amplification of ribonucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10224200A DE10224200C1 (de) 2002-05-31 2002-05-31 Vermehrung von Ribonukleinsäuren
DE10224200.3 2002-05-31

Publications (2)

Publication Number Publication Date
WO2003102232A2 WO2003102232A2 (de) 2003-12-11
WO2003102232A3 true WO2003102232A3 (de) 2004-02-12

Family

ID=27618852

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/005579 Ceased WO2003102232A2 (de) 2002-05-31 2003-05-27 Vermehrung von ribonukleinsäuren

Country Status (8)

Country Link
US (1) US20060035226A1 (de)
EP (1) EP1509623B1 (de)
JP (1) JP4372682B2 (de)
AT (1) ATE437238T1 (de)
AU (1) AU2003232835B2 (de)
CA (1) CA2487538C (de)
DE (2) DE10224200C1 (de)
WO (1) WO2003102232A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10143106C1 (de) * 2001-09-03 2002-10-10 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
FI20040723A0 (fi) * 2004-05-26 2004-05-26 Orpana Aarne Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
US8039214B2 (en) * 2007-06-29 2011-10-18 Cellscript, Inc. Synthesis of tagged nucleic acids
WO2012019168A2 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2625189B1 (de) 2010-10-01 2018-06-27 ModernaTX, Inc. Manipulierte nukleinsäuren und anwendungsverfahren dafür
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
DE19216461T1 (de) 2011-10-03 2021-10-07 Modernatx, Inc. Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon
CA3018046A1 (en) 2011-12-16 2013-06-20 Moderna Therapeutics, Inc. Modified nucleoside, nucleotide, and nucleic acid compositions
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
WO2013151665A2 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of proteins associated with human disease
PL2922554T3 (pl) 2012-11-26 2022-06-20 Modernatx, Inc. Na zmodyfikowany na końcach
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
EP3052521A1 (de) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynukleotide zur codierung von low-density-lipoprotein-rezeptor

Citations (6)

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Publication number Priority date Publication date Assignee Title
US5972607A (en) * 1996-07-16 1999-10-26 Toyo Boseki Kabushiki Kaisha Methods for nucleic acid amplification with thermostable ribonuclease H
WO2000018966A2 (en) * 1998-09-29 2000-04-06 Arch Development Corporation A new strategy for genome-wide gene analysis: integrated procedures for gene identification
US6132997A (en) * 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6361949B1 (en) * 1988-01-28 2002-03-26 Steve S. Sommer Nucleic acid amplification with direct sequencing
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
EP1275734A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
ATE159763T1 (de) * 1990-12-31 1997-11-15 Promega Corp Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen
US5256555A (en) * 1991-12-20 1993-10-26 Ambion, Inc. Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6361949B1 (en) * 1988-01-28 2002-03-26 Steve S. Sommer Nucleic acid amplification with direct sequencing
US5972607A (en) * 1996-07-16 1999-10-26 Toyo Boseki Kabushiki Kaisha Methods for nucleic acid amplification with thermostable ribonuclease H
WO2000018966A2 (en) * 1998-09-29 2000-04-06 Arch Development Corporation A new strategy for genome-wide gene analysis: integrated procedures for gene identification
US6132997A (en) * 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
EP1275734A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA

Also Published As

Publication number Publication date
CA2487538A1 (en) 2003-12-11
CA2487538C (en) 2012-07-10
JP2005532797A (ja) 2005-11-04
DE50311731D1 (de) 2009-09-03
WO2003102232A2 (de) 2003-12-11
DE10224200C1 (de) 2003-08-21
EP1509623B1 (de) 2009-07-22
AU2003232835B2 (en) 2008-03-13
ATE437238T1 (de) 2009-08-15
JP4372682B2 (ja) 2009-11-25
AU2003232835A1 (en) 2003-12-19
EP1509623A2 (de) 2005-03-02
US20060035226A1 (en) 2006-02-16

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