WO2004078956A3 - Procede nouveau pour detacher des cellules confluentes de microsupports bidimensionnels et son application pour preparer des transplantations - Google Patents

Procede nouveau pour detacher des cellules confluentes de microsupports bidimensionnels et son application pour preparer des transplantations Download PDF

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Publication number
WO2004078956A3
WO2004078956A3 PCT/IB2004/000930 IB2004000930W WO2004078956A3 WO 2004078956 A3 WO2004078956 A3 WO 2004078956A3 IB 2004000930 W IB2004000930 W IB 2004000930W WO 2004078956 A3 WO2004078956 A3 WO 2004078956A3
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WO
WIPO (PCT)
Prior art keywords
adcs
microsupports
detaching
dimensional
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2004/000930
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English (en)
Other versions
WO2004078956A2 (fr
Inventor
Alain Miller
Christophe Henry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CILBIOTECH
Original Assignee
CILBIOTECH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CILBIOTECH filed Critical CILBIOTECH
Priority to EP04717723A priority Critical patent/EP1615999A2/fr
Publication of WO2004078956A2 publication Critical patent/WO2004078956A2/fr
Anticipated expiration legal-status Critical
Publication of WO2004078956A3 publication Critical patent/WO2004078956A3/fr
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé pour détacher des cellules confluentes dépendantes de l'ancrage (ADC's) de microsupports bidimensionnels (2D-MS) sur lesquels ces ADC's sont cultivées, notamment des ADC's qui peuvent être cultivées dans un état approprié pour transplantation, telles que des ADC's dérivées de cellules pancréatiques. L'invention concerne également un procédé de préparation d'organoïdes reconstitués à partir de ces ADC's confluentes détachées qui peuvent être immortalisées selon l'invention. L'invention concerne en outre l'utilisation des organoïdes obtenus par le processus selon l'invention pour dépister des modulateurs de l'activité cellulaire ou comme un transplant thérapeutique pour leur administration à des patients nécessitant un tel traitement. Finalement, l'invention concerne un procédé de prévention ou de traitement du diabète, selon lequel des organoïdes dérivés de cellules pancréatiques selon l'invention sont transplantés dans un patient nécessitant un tel traitement.
PCT/IB2004/000930 2003-03-05 2004-03-05 Procede nouveau pour detacher des cellules confluentes de microsupports bidimensionnels et son application pour preparer des transplantations Ceased WO2004078956A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04717723A EP1615999A2 (fr) 2003-03-05 2004-03-05 Procede nouveau pour detacher des cellules confluentes de microsupports bidimensionnels et son application pour preparer des transplantations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45260103P 2003-03-05 2003-03-05
US60/452,601 2003-03-05

Publications (2)

Publication Number Publication Date
WO2004078956A2 WO2004078956A2 (fr) 2004-09-16
WO2004078956A3 true WO2004078956A3 (fr) 2006-09-21

Family

ID=32962735

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2004/000930 Ceased WO2004078956A2 (fr) 2003-03-05 2004-03-05 Procede nouveau pour detacher des cellules confluentes de microsupports bidimensionnels et son application pour preparer des transplantations

Country Status (2)

Country Link
EP (1) EP1615999A2 (fr)
WO (1) WO2004078956A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111979179B (zh) * 2020-08-24 2022-12-27 扬州大学 一种猪肝脏组织类器官模型及其体外构建方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
"Now introducing: Virtually two dimensional ...", November 2001, NUNC BRAND PRODUCTS "FLYER", XP002286805 *
A.G.SMITH ET AL.: "Successfull Sub-Culture of Human Dermal Fibroblasts and Intact HACAT Monolayers from Hydrogel Carriers", EUR CELLS MATERIALS, vol. 2002, no. Suppl.2, 2002, pages 91, XP002286802 *
CRUZ H J ET AL: "Cell-dislodging methods under serum-free conditions", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 47, no. 5, 1997, pages 482 - 488, XP002286803, ISSN: 0175-7598 *
EMING SABINE A ET AL: "Regulation of the spatial organization of mesenchymal connective tissue: Effects of cell-associated versus released isoforms of platelet-derived growth factor", AMERICAN JOURNAL OF PATHOLOGY, vol. 154, no. 1, January 1999 (1999-01-01), pages 281 - 289, XP002286804, ISSN: 0002-9440 *
GEBB C ET AL: "Harvesting and subculturing cells growing on denatured-collagen coated microcarriers (Cytodex 3).", DEVELOPMENTS IN BIOLOGICAL STANDARDIZATION. SWITZERLAND 1983, vol. 55, 1983, pages 57 - 65, XP001182316, ISSN: 0301-5149 *
GMYR V ET AL: "HUMAN PANCREATIC DUCTAL CELLS: LARGE-SCALE ISOLATION AND EXPANSION", CELL TRANSPLANTATION, ELSEVIER SCIENCE, US, vol. 10, no. 1, 2001, pages 109 - 121, XP001010271, ISSN: 0963-6897 *
GRUBER H E ET AL: "Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formation.", BMC MUSCULOSKELETAL DISORDERS [ELECTRONIC RESOURCE]. 23 OCT 2000, vol. 1, 23 October 2000 (2000-10-23), pages 1, XP002383862, ISSN: 1471-2474 *
OLIVARES ELIZABETH ET AL: "Functional integrity of dispersed islet cells attached to a two-dimensional microsupport", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 6, no. 3, September 2000 (2000-09-01), pages 313 - 314, XP009033028, ISSN: 1107-3756 *
STEINER M G ET AL: "Characterization of novel cell lines from pleomorphic adenomas of the parotid gland established in a collagen gel system.", THE LARYNGOSCOPE. MAY 1997, vol. 107, no. 5, May 1997 (1997-05-01), pages 654 - 660, XP008065008, ISSN: 0023-852X *

Also Published As

Publication number Publication date
WO2004078956A2 (fr) 2004-09-16
EP1615999A2 (fr) 2006-01-18

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