WO2005040332A3 - Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten - Google Patents

Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten Download PDF

Info

Publication number
WO2005040332A3
WO2005040332A3 PCT/EP2004/011788 EP2004011788W WO2005040332A3 WO 2005040332 A3 WO2005040332 A3 WO 2005040332A3 EP 2004011788 W EP2004011788 W EP 2004011788W WO 2005040332 A3 WO2005040332 A3 WO 2005040332A3
Authority
WO
WIPO (PCT)
Prior art keywords
bioreactor
cultivation
stimulation
mechanically
resistant cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/011788
Other languages
English (en)
French (fr)
Other versions
WO2005040332A2 (de
Inventor
Ronny Schulz
Augustinus Bader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet Leipzig
Original Assignee
Universitaet Leipzig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Leipzig filed Critical Universitaet Leipzig
Priority to JP2006536026A priority Critical patent/JP2007508830A/ja
Priority to EP04790613A priority patent/EP1675939A2/de
Priority to US10/576,618 priority patent/US20070026517A1/en
Priority to CA002543374A priority patent/CA2543374A1/en
Publication of WO2005040332A2 publication Critical patent/WO2005040332A2/de
Publication of WO2005040332A3 publication Critical patent/WO2005040332A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Der Bioreaktor (1) besteht aus einem Grundkörper, der mit einem Reaktorverschluss (21) druckdicht und steril verbunden ist und mindestens einen Reaktorraum bildet, in dem eine Ablagefläche für ein Transplantat (11) sowie ein Mini-Aktuator (14) implementiert sind. Weiterhin ist der Bioreaktor (1) mit wenigstens zwei Schlauchkupplungsanschlüssen (19) für die Mediumzufuhr und Mediumabfuhr bzw. zur Begasung versehen ist.
PCT/EP2004/011788 2003-10-21 2004-10-19 Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten Ceased WO2005040332A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006536026A JP2007508830A (ja) 2003-10-21 2004-10-19 三次元、生体及び機械的抵抗性を有する細胞移植体の培養と刺激のための方法とバイオ反応装置
EP04790613A EP1675939A2 (de) 2003-10-21 2004-10-19 Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsf higen zel ltransplantaten
US10/576,618 US20070026517A1 (en) 2004-10-19 2004-10-19 Method and bioreactor for the cultivation and stimulation of three-dimensional, vitally and mechanically reistant cell transplants
CA002543374A CA2543374A1 (en) 2003-10-21 2004-10-19 Method and bioreactor for the cultivation and stimulation of three-dimensional vital and mechanically-resistant cell transplants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10349484.7 2003-10-21
DE2003149484 DE10349484A1 (de) 2003-10-21 2003-10-21 Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten

Publications (2)

Publication Number Publication Date
WO2005040332A2 WO2005040332A2 (de) 2005-05-06
WO2005040332A3 true WO2005040332A3 (de) 2005-06-30

Family

ID=34484964

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/011788 Ceased WO2005040332A2 (de) 2003-10-21 2004-10-19 Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten

Country Status (7)

Country Link
EP (1) EP1675939A2 (de)
JP (1) JP2007508830A (de)
CN (1) CN1898375A (de)
CA (1) CA2543374A1 (de)
DE (1) DE10349484A1 (de)
RU (1) RU2370534C2 (de)
WO (1) WO2005040332A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2814472C1 (ru) * 2022-11-29 2024-02-29 Общество с ограниченной ответственностью "РЕГЕНТРОН" Биореактор для тканевой инженерии с аэрозольным способом подачи культуральной питательной среды

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005049905A1 (de) * 2005-10-17 2007-04-19 Medizinische Hochschule Hannover Verfahren und Vorrichtung zur Herstellung eines Gelenkersatzes
JP4981374B2 (ja) 2006-07-10 2012-07-18 パーパス株式会社 細胞又は組織の培養装置及び培養方法
JP5433129B2 (ja) 2006-07-10 2014-03-05 パーパス株式会社 細胞又は組織の培養方法
ITMI20071945A1 (it) * 2007-10-09 2009-04-10 Milano Politecnico Bioreattore per la generazione e la stimolazione meccanica complessa di tessuto biologico ingegnerizzato.
WO2009118140A2 (de) * 2008-03-25 2009-10-01 Novatissue Gmbh Perfundierbarer bioreaktor zur herstellung von menschlichen oder tierischen geweben
DE102008015633B4 (de) * 2008-03-25 2010-07-01 Nova Tissue Gmbh Perfundierbarer Bioreaktor zur Herstellung und/oder Kultivierung eines menschlichen oder tierischen Blutgefäßes und/oder eines menschlichen oder tierischen Gewebes
AT506826B1 (de) * 2008-05-23 2010-03-15 Greiner Bio One Gmbh Bioreaktor und verfahren zum kultivieren von zellen und geweben
DE102008050424B4 (de) 2008-10-08 2010-11-25 Universität Leipzig Verfahren und Vorrichtung zur homogenen Verteilung einer zellulären Suspension in porösem Trägermaterial für die Herstellung von vitalem biologischem Ersatzgewebe
EP2184347A3 (de) 2008-11-06 2011-05-18 Georg N. Duda Neue Komponenten und neues Bioreaktorsystem für die Kultur und mechanische Stimulation von biologischem Material
ITMI20090388A1 (it) * 2009-03-13 2010-09-14 Istituto Ortopedico Galeazzi S P A Bioreattore per la coltura di cellule o tessuti biologici, relativo metodo e sis tema multistazione
DE102009050498B4 (de) 2009-10-23 2015-08-06 Universität Leipzig Perfundierbarer Bioreaktor zur Herstellung von menschlichen und tierischen Geweben
MX344642B (es) 2009-12-29 2017-01-04 W R Grace & Co -Conn * Composiciones para formar peliculas que tienen un grado deseado de cobertura y metodos para hacer y usar las mismas.
DE102010026369B4 (de) 2010-07-07 2015-07-30 Zellwerk Gmbh Verfahren und Druck-Drehbett-Bioreaktor zur dynamischen Expansion und/oder Differenzierung von Stammzellen oder primären Zellen humanen und tierischen Ursprungs
KR101313769B1 (ko) 2011-02-28 2013-10-01 주식회사 셀루메드 동종 연조직 이식체 배양 장치
WO2012118304A2 (ko) * 2011-02-28 2012-09-07 주식회사 코리아본뱅크 동종 연조직 이식체 배양 장치
RU2482180C2 (ru) * 2011-07-14 2013-05-20 Алексей Вячеславович Ковалев Биореактор для выращивания тканеинженерных конструкций
AU2012308724A1 (en) * 2011-09-12 2014-04-24 Organovo, Inc. Platform for engineered implantable tissues and organs and methods of making the same
DE102012101078A1 (de) * 2012-02-09 2013-10-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Stimulationszelle und Verfahren zur in vitro Stimulation von Zellen oder Geweben
WO2014132101A2 (en) * 2012-10-18 2014-09-04 Yongxin Zhang Bioreactor system and methods for alternative cell culture between static and dynamic
ITFI20130220A1 (it) * 2013-09-20 2015-03-21 Azienda Ospedaliero Universitaria P Isana Apparato e processo per la preparazione di una protesi tissutale biomimetica della membrana timpanica
ITUB20160272A1 (it) * 2016-01-22 2017-07-22 Univ Degli Studi Di Palermo Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D
RU2612904C1 (ru) * 2016-04-15 2017-03-13 Евгений Александрович Тоневицкий Способ и микрофлюидный чип для культивирования клеток или клеточной модели
FR3081168B1 (fr) * 2018-05-21 2022-03-11 Univ Bordeaux Systeme de culture cellulaire en bioreacteur
DE102018122745B3 (de) * 2018-09-17 2019-12-19 Naturin Viscofan Gmbh Vorrichtung zur Medienzufuhr oder -abfuhr, Kulturgefäß mit einer solchen Vorrichtung und Verfahren zur Kultivierung von mikrobiologischen Systemen unter Verwendung eines solchen Kulturgefäßes
RU190863U1 (ru) * 2019-02-15 2019-07-15 Федеральное государственное бюджетное учреждение "Российский ордена Трудового Красного Знамени научно-исследовательский институт травматологии и ортопедии имени Р.Р. Вредена" Министерства здравоохранения Российской Федерации (ФГБУ "РНИИТО им. Р.Р. Вредена" Минздрава России) Устройство для совмещения клеточной культуры и биодеградируемого носителя
CN111705034A (zh) * 2020-06-30 2020-09-25 山东省医药生物技术研究中心（山东省病毒研究所） 一种高效促进软骨细胞增殖的方法
CN111996120B (zh) * 2020-09-25 2025-04-25 南通纺织丝绸产业技术研究院 一种带有微电流刺激功能的细胞培养装置
AT523906B1 (de) * 2020-10-02 2022-01-15 Inncellys Gmbh Inkubationskammer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039992A1 (en) * 1995-06-07 1996-12-19 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing replacement cartilage tissue constructs
DE19808055A1 (de) * 1998-02-27 1999-09-02 Adamietz Verfahren und Apparatur zur Herstellung von dreidimensionalen Gewebezellkulturen
US20010043918A1 (en) * 2000-05-05 2001-11-22 Masini Michael A. In vitro mechanical loading of musculoskeletal tissues
WO2002048317A2 (de) * 2000-12-12 2002-06-20 Bauer Joerg Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer
WO2003029398A1 (en) * 2001-08-30 2003-04-10 Takagi Industrial Co., Ltd. Cell and structure incubator

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4306661C2 (de) * 1993-03-03 1995-04-20 Michael Dipl Biol Sittinger Verfahren zum Herstellen eines Implantates aus Zellkulturen
BR0008552A (pt) * 1999-03-10 2002-05-07 Univ Pittsburgh Células tronco e redes derivadas de tecido adiposo
DE19962456A1 (de) * 1999-12-22 2001-07-12 Biotechnologie Ges Mittelhesse Verfahren und Apparatur zur Herstellung, Untersuchung und mechanischen Stimulation dreidimensionaler Gewebezellkulturen
DE10201259A1 (de) * 2002-01-15 2003-08-28 Augustinus Bader Vorrichtung zum Züchten oder Kultivieren von Zellen in einem dosenartigen Behälter

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996039992A1 (en) * 1995-06-07 1996-12-19 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing replacement cartilage tissue constructs
DE19808055A1 (de) * 1998-02-27 1999-09-02 Adamietz Verfahren und Apparatur zur Herstellung von dreidimensionalen Gewebezellkulturen
US20010043918A1 (en) * 2000-05-05 2001-11-22 Masini Michael A. In vitro mechanical loading of musculoskeletal tissues
WO2002048317A2 (de) * 2000-12-12 2002-06-20 Bauer Joerg Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer
WO2003029398A1 (en) * 2001-08-30 2003-04-10 Takagi Industrial Co., Ltd. Cell and structure incubator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2814472C1 (ru) * 2022-11-29 2024-02-29 Общество с ограниченной ответственностью "РЕГЕНТРОН" Биореактор для тканевой инженерии с аэрозольным способом подачи культуральной питательной среды

Also Published As

Publication number Publication date
JP2007508830A (ja) 2007-04-12
WO2005040332A2 (de) 2005-05-06
DE10349484A1 (de) 2005-05-25
CA2543374A1 (en) 2005-05-06
CN1898375A (zh) 2007-01-17
RU2006117360A (ru) 2007-11-27
RU2370534C2 (ru) 2009-10-20
EP1675939A2 (de) 2006-07-05

Similar Documents

Publication Publication Date Title
WO2005040332A3 (de) Verfahren und bioreaktor zum kultivieren und stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen zelltransplantaten
WO2005065419A3 (en) Method of culturing cells
WO2004106484A3 (en) Cell culturing device and system
WO2005108546A3 (en) Stirred-tank reactor system
AU3600201A (en) Method and apparatus for culturing cell or tissue
WO2005069980A3 (en) Microfluidic chemostat and a method to prevent biofilm formation with same
WO2003016507A3 (en) Generation of multipotent central nervous system stem cells
EG23843A (en) Modified pictet-spengler reaction and products prepared therefrom
WO2001021767A3 (en) Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
WO2002095187A3 (en) Methods of well treatment
AU2002343045A1 (en) A needleless injector drug capsule and a method for filling thereof
SI1625212T1 (sl) Umeten imunski organ
WO2003006613A3 (en) Methods for ex vivo propagation of somatic stem cells
IL161873A0 (en) Production of cell suspensions
ATE452180T1 (de) Blakeslea trispora zur herstellung von lycopin in hoher ausbeute, in einem geeigneten medium in abwesenheit eines exogenen corotenogenese- hemmstoffes
AU551487B2 (en) Culturing human cells in algae extract
WO2002033052A3 (de) Verfahren zur in-vitro-herstellung von vitalem, biologischem ersatzgewebe unter verwendung von zellkulturen und einem trägermaterial
WO2003084431A3 (en) Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering
MXPA05009672A (es) Metodo y dispositivo desechable para muestrear y distribuir un liquido, por ejemplo, en condiciones esteriles.
AU1380401A (en) Method for cultivating cells, a membrane module, utilization of a membrane module and reaction system for cultivation of said cells
WO2002102971A3 (en) Methods of utilizing cultured hematopoietic progenitor cells for inducing immunological tolerance
WO2002053094A8 (en) Method for detoxifying a carbohydrate containing solution
TW200500466A (en) Process for manufacturing tricholoma matsutake mycelia
Lee Jeong et al. Carbon dioxide mitigation by microalgal photosynthesis
WO2001000006A3 (en) Container for explant culture, method of using same, and process, mold and apparatus of making same

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200480038317.9

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2007026517

Country of ref document: US

Ref document number: 2543374

Country of ref document: CA

Ref document number: 2006536026

Country of ref document: JP

Ref document number: 10576618

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2004790613

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2006117360

Country of ref document: RU

WWP Wipo information: published in national office

Ref document number: 2004790613

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 10576618

Country of ref document: US

ENP Entry into the national phase

Ref document number: 2543374

Country of ref document: CA