WO2007002136A3 - Differenciation de cellules souches pluripotentes en cellules a lignage cardiomyocyte - Google Patents

Differenciation de cellules souches pluripotentes en cellules a lignage cardiomyocyte Download PDF

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Publication number
WO2007002136A3
WO2007002136A3 PCT/US2006/024060 US2006024060W WO2007002136A3 WO 2007002136 A3 WO2007002136 A3 WO 2007002136A3 US 2006024060 W US2006024060 W US 2006024060W WO 2007002136 A3 WO2007002136 A3 WO 2007002136A3
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WO
WIPO (PCT)
Prior art keywords
cells
cardiomyocyte
pluripotent stem
differentiation
primate pluripotent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/024060
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English (en)
Other versions
WO2007002136A2 (fr
WO2007002136A9 (fr
Inventor
Joseph D Gold
Mohammad Hassanipour
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geron Corp
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Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2611809A priority Critical patent/CA2611809C/fr
Priority to EP06785229.3A priority patent/EP1910518B1/fr
Priority to KR1020137021754A priority patent/KR20130099253A/ko
Priority to CN200680022866.6A priority patent/CN101228264B/zh
Priority to KR1020087001452A priority patent/KR101529317B1/ko
Priority to GB0800264A priority patent/GB2441718B/en
Priority to HK08103905.0A priority patent/HK1109913B/xx
Priority to AU2006262329A priority patent/AU2006262329B2/en
Priority to JP2008518339A priority patent/JP5560393B2/ja
Application filed by Geron Corp filed Critical Geron Corp
Publication of WO2007002136A2 publication Critical patent/WO2007002136A2/fr
Publication of WO2007002136A3 publication Critical patent/WO2007002136A3/fr
Priority to IL187611A priority patent/IL187611A/en
Anticipated expiration legal-status Critical
Publication of WO2007002136A9 publication Critical patent/WO2007002136A9/fr
Priority to US14/746,599 priority patent/US20150284684A1/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention décrit de nouveaux procédés de différenciation de cellules souches pluripotentes primates en des cellules à lignage cardiomyocyte. Les procédés consistent à utiliser la mise en culture séquentielle des cellules souches pluripotentes primates de certains facteurs de croissance pour produire des cellules à lignage cardiomyocyte. Dans certains modes de réalisation de l'invention, la population cellulaire produite par la mise en culture séquentielle est encore enrichie en cellules à lignage cardiomyocyte de manière à produire une pourcentage plus élevé de ces cellules.
PCT/US2006/024060 2005-06-22 2006-06-20 Differenciation de cellules souches pluripotentes en cellules a lignage cardiomyocyte Ceased WO2007002136A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
JP2008518339A JP5560393B2 (ja) 2005-06-22 2006-06-20 心筋細胞系列細胞への霊長類多能性幹細胞の分化
KR1020137021754A KR20130099253A (ko) 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화
CN200680022866.6A CN101228264B (zh) 2005-06-22 2006-06-20 使灵长类多能干细胞分化成心肌细胞系细胞
KR1020087001452A KR101529317B1 (ko) 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화
GB0800264A GB2441718B (en) 2005-06-22 2006-06-20 Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells
HK08103905.0A HK1109913B (en) 2005-06-22 2006-06-20 Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells
AU2006262329A AU2006262329B2 (en) 2005-06-22 2006-06-20 Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
CA2611809A CA2611809C (fr) 2005-06-22 2006-06-20 Differenciation de cellules souches pluripotentes en cellules a lignage cardiomyocyte
EP06785229.3A EP1910518B1 (fr) 2005-06-22 2006-06-20 Différenciation de cellules souches pluripotentes en cellules à lignage cardiomyocyte
IL187611A IL187611A (en) 2005-06-22 2007-11-25 A method for extracting cells from a series of cardiomyocytes from stem cells of human embryos
US14/746,599 US20150284684A1 (en) 2005-06-22 2015-06-22 Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69314105P 2005-06-22 2005-06-22
US60/693,141 2005-06-22

Publications (3)

Publication Number Publication Date
WO2007002136A2 WO2007002136A2 (fr) 2007-01-04
WO2007002136A3 true WO2007002136A3 (fr) 2007-04-19
WO2007002136A9 WO2007002136A9 (fr) 2008-03-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024060 Ceased WO2007002136A2 (fr) 2005-06-22 2006-06-20 Differenciation de cellules souches pluripotentes en cellules a lignage cardiomyocyte

Country Status (10)

Country Link
US (2) US9062289B2 (fr)
EP (1) EP1910518B1 (fr)
JP (2) JP5560393B2 (fr)
KR (2) KR101529317B1 (fr)
CN (1) CN101228264B (fr)
AU (1) AU2006262329B2 (fr)
CA (1) CA2611809C (fr)
GB (1) GB2441718B (fr)
IL (1) IL187611A (fr)
WO (1) WO2007002136A2 (fr)

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CA2611809A1 (fr) 2007-01-04
US9062289B2 (en) 2015-06-23
WO2007002136A2 (fr) 2007-01-04
CA2611809C (fr) 2018-06-19
KR101529317B1 (ko) 2015-06-16
KR20130099253A (ko) 2013-09-05
GB2441718A (en) 2008-03-12
JP5560393B2 (ja) 2014-07-23
IL187611A0 (en) 2008-03-20
CN101228264A (zh) 2008-07-23
AU2006262329A1 (en) 2007-01-04
JP2008543338A (ja) 2008-12-04
JP2013215206A (ja) 2013-10-24
KR20080030026A (ko) 2008-04-03
WO2007002136A9 (fr) 2008-03-06
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US20070010012A1 (en) 2007-01-11
IL187611A (en) 2012-12-31
EP1910518A2 (fr) 2008-04-16
AU2006262329B2 (en) 2011-04-07
EP1910518A4 (fr) 2015-03-11
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