WO2007132164A3 - Analyse de protéines - Google Patents

Analyse de protéines Download PDF

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Publication number
WO2007132164A3
WO2007132164A3 PCT/GB2007/001621 GB2007001621W WO2007132164A3 WO 2007132164 A3 WO2007132164 A3 WO 2007132164A3 GB 2007001621 W GB2007001621 W GB 2007001621W WO 2007132164 A3 WO2007132164 A3 WO 2007132164A3
Authority
WO
WIPO (PCT)
Prior art keywords
product
protein extract
proteins
analysis
modified plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/001621
Other languages
English (en)
Other versions
WO2007132164A2 (fr
Inventor
Peter Bramley
Paul Fraser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Royal Holloway and Bedford New College
Original Assignee
Royal Holloway and Bedford New College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0608604A external-priority patent/GB0608604D0/en
Priority claimed from GB0608602A external-priority patent/GB0608602D0/en
Application filed by Royal Holloway and Bedford New College filed Critical Royal Holloway and Bedford New College
Publication of WO2007132164A2 publication Critical patent/WO2007132164A2/fr
Publication of WO2007132164A3 publication Critical patent/WO2007132164A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un procédé de détermination de la présence, dans un produit, d'une matière dérivée d'une plante génétiquement modifiée. Le procédé consiste à utiliser un extrait de protéine provenant du produit; à enrichir l'extrait de protéine; à digérer l'extrait de protéine au moyen d'une enzyme; et à détecter la présence ou l'absence d'au moins un peptide marqueur produit par la digestion enzymatique d'une protéine transgénique, ce qui permet de déterminer si la matière dérivée d'une plante génétiquement modifiée est présente dans le produit.
PCT/GB2007/001621 2006-05-02 2007-05-02 Analyse de protéines Ceased WO2007132164A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0608604A GB0608604D0 (en) 2006-05-02 2006-05-02 Mass spectrometric analysis of proteins
GB0608602.9 2006-05-02
GB0608604.5 2006-05-02
GB0608602A GB0608602D0 (en) 2006-05-02 2006-05-02 Analysis of proteins

Publications (2)

Publication Number Publication Date
WO2007132164A2 WO2007132164A2 (fr) 2007-11-22
WO2007132164A3 true WO2007132164A3 (fr) 2008-03-27

Family

ID=38441488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/001621 Ceased WO2007132164A2 (fr) 2006-05-02 2007-05-02 Analyse de protéines

Country Status (1)

Country Link
WO (1) WO2007132164A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA112515C2 (uk) * 2009-06-03 2016-09-26 Дау Аґросаєнсиз Елелсі Спосіб виявлення присутності двох або більше білків, що становлять інтерес, в зразках рослинного походження
WO2014010700A1 (fr) * 2012-07-12 2014-01-16 国立大学法人九州大学 Composé amino, procédé de spectrométrie de masse à sensibilité élevée pour celui-ci et procédé de dosage d'un biomarqueur
CN103698418B (zh) * 2013-11-12 2015-07-08 北京理工大学 植物中转基因蛋白cp4-epsps的定量检测方法
DE102014008135A1 (de) * 2014-06-06 2015-12-17 Gfl Gesellschaft Für Lebensmittel-Forschung Mbh Verfahren zur analytischen Authentizitätprüfung von Inhaltsstoffen in Lebensmitteln unter Verwendung künstlicher Antikörper
US20150355189A1 (en) * 2014-06-10 2015-12-10 Dow Agrosciences Llc Quantitative analysis of transgenic protein 5-enolpyruvylshikimate-3-phosphate synthase
CN106841458A (zh) * 2017-03-17 2017-06-13 中国肉类食品综合研究中心 液相色谱串联质谱测定肉类食品中植物源性成分的方法
CN109521105A (zh) * 2018-08-03 2019-03-26 西北工业大学 一种用于出土面食文物的鉴定方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001098523A2 (fr) * 2000-06-20 2001-12-27 Gd Biotech Co., Ltd Procede de detection d'aliments obtenus par recombinaison genetique, et trousse pour effectuer cette detection
WO2002084250A2 (fr) * 2001-04-17 2002-10-24 Femtolink Biotechnologies Llc Procedes de detection par spectrometrie de masse et quantification de proteines cibles specifiques dans des echantillons biologiques complexes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001098523A2 (fr) * 2000-06-20 2001-12-27 Gd Biotech Co., Ltd Procede de detection d'aliments obtenus par recombinaison genetique, et trousse pour effectuer cette detection
WO2002084250A2 (fr) * 2001-04-17 2002-10-24 Femtolink Biotechnologies Llc Procedes de detection par spectrometrie de masse et quantification de proteines cibles specifiques dans des echantillons biologiques complexes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KREKEL F ET AL: "SUBSTRATE AND INHIBITOR-INDUCED CONFORMATIONAL CHANGES IN THE STRUCTURALLY RELATED ENZYMES UDP-N-ACETYLGLUCOSAMINE ENOPYRUVYL TRANSFERASE (MURA) AND 5-ENOLPYRUVYLSHIKIMATE 3-PHOSPHATE SYNTHASE (EPSP)", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 38, no. 28, 1999, pages 8864 - 8878, XP002931371, ISSN: 0006-2960 *
OCANA MIREIA FERNANDEZ ET AL: "Mass spectrometric detection of CP4 EPSPS in genetically modified soya and maize", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 21, no. 3, 2007, pages 319 - 328, XP002451539, ISSN: 0951-4198 *

Also Published As

Publication number Publication date
WO2007132164A2 (fr) 2007-11-22

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