WO2008132247A8 - Behälter und verfahren zum nachweis von fluoreszenz - Google Patents

Behälter und verfahren zum nachweis von fluoreszenz Download PDF

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Publication number
WO2008132247A8
WO2008132247A8 PCT/EP2008/058302 EP2008058302W WO2008132247A8 WO 2008132247 A8 WO2008132247 A8 WO 2008132247A8 EP 2008058302 W EP2008058302 W EP 2008058302W WO 2008132247 A8 WO2008132247 A8 WO 2008132247A8
Authority
WO
WIPO (PCT)
Prior art keywords
light
sensor surface
area
receptacle
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/058302
Other languages
English (en)
French (fr)
Other versions
WO2008132247A2 (de
WO2008132247A3 (de
Inventor
Thomas Ruckstuhl
Stefan Seeger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP08761398A priority Critical patent/EP2227684A2/de
Priority to JP2010504761A priority patent/JP2011525612A/ja
Priority to AU2008244225A priority patent/AU2008244225A1/en
Priority to US12/598,397 priority patent/US20100136709A1/en
Priority to CN2008800143192A priority patent/CN101910823A/zh
Priority to CA002685574A priority patent/CA2685574A1/en
Publication of WO2008132247A2 publication Critical patent/WO2008132247A2/de
Anticipated expiration legal-status Critical
Publication of WO2008132247A8 publication Critical patent/WO2008132247A8/de
Publication of WO2008132247A3 publication Critical patent/WO2008132247A3/de
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/064—Stray light conditioning
    • G01N2201/0642—Light traps; baffles
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B2207/00—Coding scheme for general features or characteristics of optical elements and systems of subclass G02B, but not including elements and systems which would be classified in G02B6/00 and subgroups
    • G02B2207/113—Fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Die Erfindung betrifft einen Flüssigkeitsbehälter (22), umfassend einen Boden (1) und Seitenwände zum Halten einer Flüssigkeit, wobei der Boden (1) umfasst: eine ebene Sensoroberfläche (3), die bei Befüllung mit der Flüssigkeit in Kontakt steht; eine Lichteintrittsfläche (2) unterhalb der Sensoroberfläche (3), die geeignet ist, Licht (13) auf die Sensoroberfläche (3) zu fokussieren; eine Lichtaustrittsfläche (5); und eine Mantelfläche (4), die geeignet ist, Licht von der Sensoroberfläche (3) zu reflektieren, so dass es durch die Lichtaustrittsfläche (5) austreten kann. Weiterhin betrifft die Erfindung ein Verfahren zur qualitativen oder quantitativen Analytbestimmung in einem derartigen Flüssigkeitsbehälter (22) wobei Anregungslicht (13) über die Lichteintrittsfläche (2) auf die Sensoroberfläche (3) fokussiert wird, hierdurch ein den Analyten kennzeichnender Lumineszenzmarker angeregt wird, und dann die so entstehende Lumineszenz an der Manteloberfläche (4) reflektiert und nach Austritt durch die Lichtaustrittsfläche (5) detektiert wird. Die Erfindung betrifft auch eine Analysevorrichtung, umfassend eine Halterung für einen Flüssigkeitsbehälter; eine Lichtquelle (10), die derart angeordnet ist, dass ihr Licht (13) über die Lichteintrittsfläche (2) auf die Sensoroberfläche (3) des Flüssigkeitsbehälters (22) fokussiert werden kann,- sowie einen Detektor (17), der derart angeordnet ist, dass er das aus der Lichtaustrittsfläche (5) des Flüssigkeitsbehälters (22) austretende Licht (9) detektieren kann.
PCT/EP2008/058302 2007-04-30 2008-06-27 Behälter und verfahren zum nachweis von fluoreszenz Ceased WO2008132247A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP08761398A EP2227684A2 (de) 2007-04-30 2008-06-27 Behälter und verfahren zum nachweis von fluoreszenz
JP2010504761A JP2011525612A (ja) 2007-04-30 2008-06-27 蛍光検出のための容器及び方法
AU2008244225A AU2008244225A1 (en) 2007-04-30 2008-06-27 Receptacle, and method for the detection of fluorescence
US12/598,397 US20100136709A1 (en) 2007-04-30 2008-06-27 Receptacle and method for the detection of fluorescence
CN2008800143192A CN101910823A (zh) 2007-04-30 2008-06-27 用于荧光检测的容器与方法
CA002685574A CA2685574A1 (en) 2008-06-27 2008-06-27 Receptacle and method for the detection of fluorescence

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007020610A DE102007020610A1 (de) 2007-04-30 2007-04-30 Behälter und Verfahren zum Nachweis von Fluoreszenz
DE102007020610.2 2007-04-30

Publications (3)

Publication Number Publication Date
WO2008132247A2 WO2008132247A2 (de) 2008-11-06
WO2008132247A8 true WO2008132247A8 (de) 2010-01-07
WO2008132247A3 WO2008132247A3 (de) 2010-03-11

Family

ID=39868588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/058302 Ceased WO2008132247A2 (de) 2007-04-30 2008-06-27 Behälter und verfahren zum nachweis von fluoreszenz

Country Status (7)

Country Link
US (1) US20100136709A1 (de)
EP (1) EP2227684A2 (de)
JP (1) JP2011525612A (de)
CN (1) CN101910823A (de)
AU (1) AU2008244225A1 (de)
DE (1) DE102007020610A1 (de)
WO (1) WO2008132247A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102687062B (zh) * 2009-12-21 2016-08-03 马丁专业公司 具有多个光源的投影照明装置
GB2480293A (en) * 2010-05-12 2011-11-16 Univ Dublin City A luminescence based sensor
JP5669087B2 (ja) * 2010-09-28 2015-02-12 国立大学法人九州工業大学 蛍光測定方法及び蛍光測定装置
DE102012102983A1 (de) 2012-04-05 2013-10-10 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen eines kritischen Winkels eines Anregungslichtstrahls
EP3227664B1 (de) * 2014-12-05 2020-09-16 Bacterioscan Ltd. Mehfachabtastendes laserstreuungsmessinstrument mit inkubationsfunktion und systeme zur verwendung davon
KR102494654B1 (ko) 2015-02-06 2023-01-31 라이프 테크놀로지스 코포레이션 생물학적 시료 평가 시스템과 방법
US20170108435A1 (en) * 2015-10-14 2017-04-20 University Of Alaska, Fairbanks Fluorometer
TWI789343B (zh) * 2016-02-01 2023-01-11 丹麥商碩騰丹麥有限公司 微流體分析系統、執行分析的微流體匣及方法
DE102017115661A1 (de) 2017-07-12 2019-01-17 Endress+Hauser Conducta Gmbh+Co. Kg Optischer Sensor
CN107457017A (zh) * 2017-08-17 2017-12-12 北京诺亚威仪器仪表有限公司 一种试剂检测管
DE102017223851B4 (de) * 2017-12-28 2020-08-06 Biochip Systems GmbH Sensoranordnung zur Detektion wenigstens einer stofflichen Eigenschaft einer Probe sowie Mikrotiter-Platte mit einer Vielzahl von Sensoranordnungen
DE102018202588A1 (de) * 2018-02-21 2019-08-22 Robert Bosch Gmbh Optische Sensorvorrichtung sowie ein Verfahren zur Herstellung einer optischen Sensorvorrichtung
US20240151584A1 (en) * 2022-11-07 2024-05-09 Endress+Hauser Optical Analysis, Inc. Flowcell and system with improved collection efficiency for raman spectroscopy

Family Cites Families (19)

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FI64862C (fi) * 1982-02-05 1984-01-10 Kone Oy Foerfarande foer fotometrisk maetning av vaetskor i reaktionskaerl och reaktionskaerl
DE3424108A1 (de) * 1984-06-29 1986-01-09 Bernhard Prof. Dr.-Ing. 4300 Essen Schrader Probenanordnung zur spektrometrie, verfahren zur messung von lumineszenz und streuung und verwendung der probenanordnung
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US5192510A (en) * 1991-01-30 1993-03-09 E. I. Du Pont De Nemours And Company Apparatus for performing fluorescent assays which separates bulk and evanescent fluorescence
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Also Published As

Publication number Publication date
DE102007020610A1 (de) 2008-11-20
CN101910823A (zh) 2010-12-08
AU2008244225A1 (en) 2008-11-06
EP2227684A2 (de) 2010-09-15
WO2008132247A2 (de) 2008-11-06
US20100136709A1 (en) 2010-06-03
JP2011525612A (ja) 2011-09-22
WO2008132247A3 (de) 2010-03-11

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