WO2009136742A1 - Transistors fonctionnalisés en récepteurs olfactifs pour nez bioélectronique hautement sélectif, et biocapteur utilisant de tels transistors - Google Patents

Transistors fonctionnalisés en récepteurs olfactifs pour nez bioélectronique hautement sélectif, et biocapteur utilisant de tels transistors Download PDF

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WO2009136742A1
WO2009136742A1 PCT/KR2009/002401 KR2009002401W WO2009136742A1 WO 2009136742 A1 WO2009136742 A1 WO 2009136742A1 KR 2009002401 W KR2009002401 W KR 2009002401W WO 2009136742 A1 WO2009136742 A1 WO 2009136742A1
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nanostructure
olfactory receptor
substrate
receptor protein
odorants
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Seung-Hun Hong
Tai Hyun Park
Tae-Hyun Kim
Sang Hun Lee
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Seoul National University Industry Foundation
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Seoul National University Industry Foundation
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Priority to US12/991,609 priority Critical patent/US8377706B2/en
Publication of WO2009136742A1 publication Critical patent/WO2009136742A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
    • H10K10/00—Organic devices specially adapted for rectifying, amplifying, oscillating or switching; Organic capacitors or resistors having potential barriers
    • H10K10/40—Organic transistors
    • H10K10/46—Field-effect transistors, e.g. organic thin-film transistors [OTFT]
    • H10K10/462—Insulated gate field-effect transistors [IGFETs]
    • H10K10/466—Lateral bottom-gate IGFETs comprising only a single gate
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
    • H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
    • H10K85/20—Carbon compounds, e.g. carbon nanotubes or fullerenes
    • H10K85/221—Carbon nanotubes

Definitions

  • the present invention relates to a transistor and a biosensor using the same and, more particularly, to an olfactory receptor-functionalized transistor useful for a highly selective bioelectronic nose, and a biosensor using the same.
  • Electrode refers to a system which may reproduce a human nose using sensor arrays and pattern recognition systems.
  • the capabilities of the electronic noses thus developed are still inferior to thoseof the human olfactory system in terms of specificity.
  • the initial step is to bind specific odorants to the olfactory receptor protein which triggers signal transduction in a cell.
  • Olfactory receptors expressed in the cell membranes of olfactory receptor neurons are responsible for the detection of odorant molecules. That is, when the odorants bind to the olfactory receptors as described above, the receptors are activated.
  • the activated olfactory receptors are the initial player in a signal transduction cascade which ultimately produces a nerve impulse which is transmitted to the brain.
  • GPCRs G protein-coupled receptors
  • swCNT-FETs single-walled carbon nanotube-field effect transistors
  • swCNT-FETs have not been usedin the application of the bioelectronic nose.
  • olfactory receptors are G protein-coupled receptors (GPCRs) which may maintain their functionality only as a part of the cell membranes, as described above.
  • GPCRs G protein-coupled receptors
  • lipid membranes containing functional olfactory receptors should be formed on swCNT-FETs to build a bioelectronic nose, which is still a very difficult task.
  • An embodiment of the present invention is directed to providing an olfactory receptor-functionalized transistor useful for a bioelectronic nose which may detect and analyze specific odorants with high selectivity by functionalizing a nanostructure transistor withan olfactory receptor, a method for manufacturing the transistor, a biosensor using the same, and a method for detecting odorants using the biosensor.
  • a transistor including: a substrate; a source electrode and a drain electrode formed being spaced apart from each other on the substrate; a nanostructure electrically contacted with and formed between the source electrode and the drain electrode; and a lipid membrane having an olfactory receptor protein which is formed to cover surfaces of the source electrode, the drain electrode, and the nanostructure.
  • a method for manufacturing a transistor including: forming a nanostructure on a substrate; forming a source electrode and a drain electrode to be spaced apart from each other and electrically contact the nanostructure on the substrate; and forming a lipid membrane having an olfactory receptor protein to cover surfaces of the nanostructure, the source electrode, and the drain electrode.
  • a biosensor for detecting odorants to be bound to the olfactory receptor protein by using the transistor for detecting odorants to be bound to the olfactory receptor protein by using the transistor.
  • a method for detecting odorants using the biosensor including: exposing odorants to the biosensor; and measuring a conductance modulation generated by binding the odorants to the olfactory receptor protein of the biosensor.
  • the significantly improved selectivity and sensitivity in detection of odorants may be achieved by functionalizing a nanostructure transistor with an olfactory receptor.
  • the olfactory receptor-functionalized transistor is a biosensor useful fora bioelectronic nose which can detect odorants highly specifically with femtomolar sensitivity, and may be applied in various fields requiring the rapid detection of specific odorants, for example, anti-bioterrorism, disease diagnostics, and food safety.
  • large-scale and diverse sensor arrays for sensitive and selective multiplexed detection of various odorants may be also realized with a rapid and high-throughput.
  • the present invention may provide a novel and powerful platform for development of novel pharmaceuticals and perfumes based on the capability of monitoring GPCR operation in real time.
  • Figs. 1 to 4 show schematic views of a method for manufacturing a transistor in accordance with one example of the present invention.
  • Fig. 5 shows a schematic view of a mechanism for detection of odorants in accordance with one example of the present invention.
  • Fig. 6 shows a Western blot analysis of hOR2AG1 in accordance with Example 1.
  • Fig. 7 shows a transmission electron microscope (TEM) photograph of the immunogold-labled portions of hOR2AG1 in accordance with Example 1.
  • Fig. 8 shows a schematic view of a method for measuring the effects of odorants in accordance with Example 3.
  • Fig. 9 shows the molecular structures of odorants used in Examples.
  • Fig. 10 shows a graph of a real time conductance measurement of the biosensor of the present invention after amyl butyrate was introduced at various concentrations.
  • Fig. 11 shows a graph of a real time conductance measurement of the biosensor of the present invention after butyl butyrate, propyl butyrate, and pentyl valerate were introduced at 100 ⁇ M and amyl butyrate was introduced at 1 pM.
  • Figs. 12 and 13 show graphs of a real time conductance measurement of the biosensors using modified carbon nanotube transistors with lipid membranes in which hOR2AG1 is not included and bare carbon nanotube transistors, respectively.
  • Figs. 14 to 16 show graphs of a real time conductance measurement of the biosensors after butyl butyrate, pentyl valerate, and propyl butyrate were introduced at various concentrations, respectively, in accordance with Example 5.
  • a transistor in one embodiment, includes a substrate; a source electrode and a drain electrode formed being spaced apart from each other on the substrate; a nanostructure electrically contacted with and formed between the source electrode and the drain electrode; and a lipid membrane having an olfactory receptor protein which is formed to cover surfaces of the source electrode, the drain electrode, and the nanostructure.
  • the lipid membrane having an olfactory receptor protein is formed to wholly cover the surfaces of the source and drain electrodes formed on the substrate and the surface of the nanostructure formed between the electrodes.
  • the olfactory receptor protein belongs to a family of G-protein coupled receptors and may exist over the surface of, the interior of, or the surface and interior of a lipid double membrane.
  • An olfactory receptor membrane generally includes a ionizable cysteine residue and exists in a conformational equilibrium between biophysically activated and non-activated states.
  • the activated and non-activated states of the olfactory receptor molecule are associated with a negatively-charged base form and a neutral acid form of cysteine, respectively.
  • odorant molecules may be detected highly selectively based on electrostatic perturbation of a nanostructure junction generated from a conformational change by binding the odorants to the olfactory receptor molecules.
  • the olfactory receptor proteins are the largest family of GPCR which is the most ubiquitous class of drug targets and up to 50% of current drugs are targeted at GPCR.
  • GPCR the most ubiquitous class of drug targets
  • a highly-specific detection of odorants with femtomolar sensitivity may be achieved in real time, and various and novel applications such as a highly selective artificial nose application and development of novel pharmaceuticals and perfumes may be achieved.
  • the nanostructure may be at least one form selected from the group consisting of nanotube, nanowire, nanorod, nanoribbon, nanofilm, and nanoball.
  • semiconductor nanowires such as silicon nanowires, and carbon nanotubes may be used, and a single-walled carbon nanotube is especially preferable in terms of high biocompatibility and device characteristics.
  • the substrate may be at least one selected from the group consisting of silicon, glass, quartz, metal, plastic and oxide.
  • the source and drain electrodes may be formed of at least one metal selected from the group consisting of platinum, gold, chrome, copper, aluminum, nickel, palladium, and titanium.
  • a method for manufacturing a transistor includes forming a nanostructure on a substrate; forming a source electrode and a drain electrode to be spaced apart from each other and electrically contact the nanostructure on the substrate; and forming a lipid membrane having an olfactory receptor protein to cover surfaces of the nanostructure, the source electrode, and the drain electrode.
  • Said forming a nanostructure on the substrate may include patterning a self-assembled monolayer consisting of a molecule with a interface energy to the nanostructure higher than that of the substrate surface, on the substrate surface, immersing or exposing the patterned substrate in a nanostructure-containing solution or to a nanostructure-containing gas, and selectively adsorbing the nanostructure on a bare surface portion of the substrate on which the self-assembled monolayer is not formed.
  • Non-limiting examples for forming the nanostructure are described in Korean Patent No. 10-736361, which is hereby incorporated by reference.
  • Said forming a lipid membrane having an olfactory receptor protein to cover surfaces of the nanostructure, the source electrode, and the drain electrode may include spreading a solution of lipid membrane having the olfactory receptor protein on surfaces of the nanostructure, the source electrode, and the drain electrode, vacuum-drying the solution, and fixing the olfactory receptor protein.
  • the lipid membrane having an olfactory receptor protein is hereby formed to wholly cover the surfaces of the source and drain electrodes formed on the substrate and the surface of the nanostructure formed between the electrodes.
  • the processes may be applied in detection of odorants by fixing the olfactory receptor protein through reducing complex procedures and simplifying manipulations.
  • a solution of lipid membrane having the olfactory receptor protein may be a membrane fraction having the olfactory receptor protein.
  • the time for vacuum drying may be suitably selected to fix the olfactory receptor protein in accordance with manufacturing conditions, and may be, for example, about 1 hour to about 10 hours, preferably about 3 hours to 5 hours.
  • a method for manufacturing a transistor 1 is specifically described in accordance with one Example of the present invention.
  • a self-assembled monolayer 11 consisting of a molecule with interface energy to the nanostructure higher than that of the surface of the substrate 10, on the substrate 10 surface.
  • a self-assembled monolayer consisting of at least one molecule selected from the group consisting of hydrophobic molecules, especially, octadecyltrichlorosilane (OTS), octadecyltrimethoxysilane (OTMS), octadecyltriethoxysilane (OTE), and octadecanethiol (ODT) may be patterned.
  • SAM self-assembled monolayer
  • the patterning method is not specifically limited, and may include, for example, microcontact printing, photolithography, dip-pen nanolithography, e-beam lithography, ion-beam lithography, nanografting, nanoshaving, or STM lithography.
  • a nanostructure 12 When the thus patterned substrate 10 is immersed in a nanostructure 12-containing solution or exposed to a nanostructure 12-containing gas, a nanostructure 12 may be selectively adsorbed and formed on a bare surface portion of the substrate on which the self-assembled monolayer 11 is not formed, as described in Fig. 2.
  • a source electrode 13 and a drain electrode 14 are formed to be spaced apart from each other and electrically contact the nanostructure 12 formed on the surface of the substrate 10.
  • the electrode formation method may be appropriately selected from methods generally known in the art, and include photolithography, physical vapor deposition (PVD), e-beam evaporation, or thermal evaporation.
  • PVD physical vapor deposition
  • e-beam evaporation e-beam evaporation
  • thermal evaporation e-beam evaporation
  • a lipid membrane 16 having the olfactory receptor protein 15 is formed to cover surfaces of the nanostructure 12, the source electrode 13, and the drain electrode 14. These processes may be achieved by spreading a lipid membrane 16 having the olfactory receptor protein 15-containing solution on surfaces of the nanostructure 12, the source electrode 13, and the drain electrode 14, vacuum-drying the solution, and fixing the olfactory receptor protein 16.
  • said exposing odorants to the biosensor of the present invention may be performed by contacting an odorant-containing solution or an odorant-containing gas with the biosensor.
  • a method for detecting odorants in accordance with one embodiment includes, after an odorant-containing solution is dripped on and contacted with the biosensor, measuring a conductance modulation generated by binding odorants to the olfactory receptor protein of the biosensor.
  • a method for detecting odorants in accordance with embodiment includes, after an odorant-containing gas is exposed to and contacted with the biosensor, measuring a conductance modulation generated by binding odorants to the olfactory receptor protein of the biosensor.
  • the olfactory receptor proteins are the largest family of GPCR, at which up to 50% of current drugs are targeted, and thus a detection of odorants with high selectivity, sensitivity, and specificity may be achieved in real time in accordance with the embodiment of the present invention.
  • Example 1 Preparation, immunoblot analysis, and electron microscopy of an olfactory receptor protein
  • human olfactory receptor 2AG1 (hOR2AG1) protein was used as an olfactory receptor protein.
  • the hOR1AG1 protein is a fusion protein with a glutathione-S-transferase (GST)-tag at the N-terminus, which is expressed in Escherichia coli ( E. coli ).
  • GST glutathione-S-transferase
  • the cultured cells were centrifuged at 7,000 g for 30 min, harvested, and resuspended in 1 ml of phosphate buffered saline (PBS).
  • PBS phosphate buffered saline
  • the resuspended cells were dissolved by an ultrasonic treatment for 5 min, and insoluble fractions including membrane fractions and cell debris were centrifuged at 15,000 g for 30 min and collected. Subsequently, to remove membrane-incorporated proteins other than the hOR1AG1, insoluble fractions including the hOR2AG1 were incubated in a solution containing 2 vol% Triton X-100, and then membrane fractions containing hOR2AG1 were collected by centrifuge.
  • the expression of the hOR2AG1 in E. coli was identified by a Western Blot Analysis. Insoluble fractions were separated by electrophoresis using sodium dodecylsulfate (SDS)-polyacrylamide gels, and then transferred to a polyvinylidene fluoride membrane (PVDF) (Bio-Rad, CA, USA). The membrane was blocked with 5 wt% skimmed milk in PBS containing 0.1 vol% Tween-20 (PBST, pH 7.4). The membrane was incubated with anti-GST antibody (Santa Cruz, CA, USA) and antibody conjugated with horseradish peroxidase (Amersham-Pharmacia Biotech, UK). Blots are shown in Fig. 6 by using an enhanced chemiluminescence detection kit (Amersham-Pharmacia Biotech, UK).
  • Fig. 6 shows a Western Blot Analysis to identify the expression of the hOR2AG1 protein, with a cytosol fraction in lane 1 and an insoluble fraction in lane 2.
  • the protein molecules were bound together as a bimolecular or a trimolecular group in some cases. Polymerized forms such as dimers and trimers occur with monomers of the hOR2AG1.
  • the insoluble fraction may contain a membrane-integrated form and a inclusion body form.
  • E. coli cells were fixed with modified Karnovsky's fixative consisting of 0.5 vol% glutaraldehyde and 4 vol% paraformaldehyde in 0.05 M sodium cacodylate buffer (pH 7.2) for 2 hours, and then were embedded in LR white resin.
  • An ultrathin section of E. coli (thickness: about 60 nm) loaded on the TEM grid was treated with a blocking solution containing 1 wt% bovine serum albumin (BSA) in tris-buffered saline (TBS, pH 7.2) for 30 min. The grids were incubated in the anti-GST antibody inthe blocking solution.
  • BSA bovine serum albumin
  • swCNTs purified single-walled carbon nanotubes (swCNTs) (Carbon Nanotechnologies) were dispersed in 1,2-dichlorobenzene with ultrasonic vibration for 20 min to prepare a swCNT suspension.
  • concentration of the swCNT suspension was 0.1 mg/ml or less.
  • OTS octadecyltrichlorosilane
  • SAM self-assembled monolayer
  • Example 1 1 ⁇ l of the hOR2AG1-containing fraction collected in Example 1 was evenly spread to cover the swCNTs and electrodes, and vacuum-dried for 4 hours to fix the hOR2AG1, thereby manufacturing human olfactory receptor-functionalized carbon nanotube transistors.
  • Example 3 Measurement of effects of odorants on biosensor using human olfactory receptor-functionalized transistors of the present invention
  • Fig. 9 shows four similar odorants (amyl butyrate (AB), butyl butyrate (BB), propyl butyrate(PB), and pentyl valerate (PV)) used for characterization of sensor reactions.
  • AB myl butyrate
  • BB butyl butyrate
  • PB propyl butyrate
  • PV pentyl valerate
  • a 1 M stock solution of the odorants was prepared, and additional dilutions (from 10 -1 M to 10 -13 M) were obtained by serial 1:10 dilutions in PBS.
  • the hOR2AG1 is known to be activated by amyl butyrate (AB), the most common reagent for fruit flavor.
  • Fig. 10 shows a time dependence of the source-drain current of the biosensor after the introduction of amyl butyrate (AB) at various concentrations such as 100 fM, 1 pM, 10 pM, and 100 pM.
  • the source-drain current was sharply decreased by the addition of amyl butyrate (AB), an odorant known to activate the hOR2AG1, and then gradually saturated at lower values.
  • amyl butyrate (AB) induced the binding of amyl butyrate (AB) to the reactive domain of hOR2AG1, causing a gradual saturation.
  • the detection of solutions at 100 fM was enabled, meaning that at least 10-fold sensitivity was achieved compared to conventional bioelectronic nose systems using various transducers.
  • BB butyl butyrate
  • PB propyl butyrate
  • PV pentyl valerate
  • Amyl butyrate was introduced on modified carbon nanotube transistors with lipid membranes in which hOR2AG1 was not included (identical to those manufactured in Example 2 except that lipid membranes were formed but do not have hOR2AG1) and bare carbon nanotube transistors (identical to those manufactured in Example 2 except that lipid membranes having hOR2AG1 were not formed) to measure each of the conductance modulations.
  • the results are shown in Figs. 12 and 13, respectively.
  • each of the conductance modulations was observed on the biosensors using modified carbon nanotube transistors with lipid membranes in which hOR2AG1 was not included and bare carbon nanotube transistors, after the introduction of amyl butyrate at 100 mM. From the observations, it may be identified that for detection limits, 10 12 -fold higher concentrations were recorded when compared to the biosensors using hOR2AG1-functionalized transistors (See Fig. 10).
  • Table 1 shows the detection limits of the biosensors using carbon nanotube transistors for amyl butyrate in accordance with different surface modifications of carbon nanotube transistors as described above.
  • Butyl butyrate, phenyl valerate, and propyl butyrate were introduced at various concentrations on the biosensors using hOR2AG1 functionalized transistors manufactured in Example 2 of the present invention, each of the conductance modulations was measured, and the results were recorded in Figs. 14 to 16, respectively.
  • the arrows illustrated in Figs. 14 to 16 show time points of introduction of each odorant.

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Abstract

La présente invention concerne, pour l'un de ses aspects, un transistor comprenant: un substrat; une électrode source et une électrode collectrice séparées l'une de l'autre sur le substrat; une nanostructure disposée en contact électrique entre les deux électrodes; et une membrane de lipide contenant une protéine récepteur olfactif et recouvrant les surfaces des deux électrodes et de la nanostructure. Le transistor fonctionnalisé en récepteur olfactif selon un aspect de l'invention convient pour un nez bioélectronique capable, à un niveau de sensibilité femtomolaire, de détecter de façon très spécifique des matières odorantes. Les domaines d'application de ce transistor sont essentiellement ceux qui demandent une détection rapide de matières odorantes spécifiques, notamment, notamment les domaines de la lutte contre le terrorisme biologique, du diagnostic de maladies, et de la sécurité alimentaire.
PCT/KR2009/002401 2008-05-07 2009-05-07 Transistors fonctionnalisés en récepteurs olfactifs pour nez bioélectronique hautement sélectif, et biocapteur utilisant de tels transistors Ceased WO2009136742A1 (fr)

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CN101783367A (zh) * 2010-02-11 2010-07-21 复旦大学 一种基于三五族元素的纳米线mos晶体管及其制备方法
WO2012131644A1 (fr) * 2011-03-30 2012-10-04 Centre National De La Recherche Scientifique Procede de formation de motifs d'objets sur la surface d'un substrat
WO2012139526A1 (fr) * 2011-04-14 2012-10-18 上海交通大学医学院 Procédé pour la détection de matières odorantes formant une liaison de coordination avec des métaux
CN106290519A (zh) * 2016-08-30 2017-01-04 上海大学 氮掺杂碳纳米管复合l‑半胱氨酸修饰的玻碳电极的制备方法及其应用
RU2713099C1 (ru) * 2019-07-15 2020-02-03 Александр Евгеньевич Кузнецов Устройство для обнаружения и распознавания аналитов в многокомпонентной среде и способ его изготовления
AU2017383462B2 (en) * 2016-12-21 2023-05-25 Scentian Bio Limited Sensor device and methods
CN116660347A (zh) * 2023-05-15 2023-08-29 上海交通大学 一种仿生嗅觉传感器及其制备方法和应用

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WO2006110347A2 (fr) * 2005-03-29 2006-10-19 The Trustees Of The University Of Pennsylvania Nanotubes de carbone a paroi simple fonctionnellement adsorbes sur des biopolymeres, destines a etre utilises comme capteurs chimiques
US7342479B2 (en) * 2003-04-28 2008-03-11 Eikos, Inc. Sensor device utilizing carbon nanotubes

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US7342479B2 (en) * 2003-04-28 2008-03-11 Eikos, Inc. Sensor device utilizing carbon nanotubes
US20060204428A1 (en) * 2005-01-24 2006-09-14 The Regents Of The University Of California Lipid nanotube or nanowire sensor
WO2006110347A2 (fr) * 2005-03-29 2006-10-19 The Trustees Of The University Of Pennsylvania Nanotubes de carbone a paroi simple fonctionnellement adsorbes sur des biopolymeres, destines a etre utilises comme capteurs chimiques

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101783367A (zh) * 2010-02-11 2010-07-21 复旦大学 一种基于三五族元素的纳米线mos晶体管及其制备方法
WO2012131644A1 (fr) * 2011-03-30 2012-10-04 Centre National De La Recherche Scientifique Procede de formation de motifs d'objets sur la surface d'un substrat
WO2012139526A1 (fr) * 2011-04-14 2012-10-18 上海交通大学医学院 Procédé pour la détection de matières odorantes formant une liaison de coordination avec des métaux
CN103608674A (zh) * 2011-04-14 2014-02-26 上海交通大学医学院 一种配位金属的气味化合物的探测方法
CN106290519A (zh) * 2016-08-30 2017-01-04 上海大学 氮掺杂碳纳米管复合l‑半胱氨酸修饰的玻碳电极的制备方法及其应用
AU2017383462B2 (en) * 2016-12-21 2023-05-25 Scentian Bio Limited Sensor device and methods
AU2023219922B2 (en) * 2016-12-21 2024-05-09 Scentian Bio Limited Sensor device and methods
US12044651B2 (en) 2016-12-21 2024-07-23 Scentian Bio Limited Sensor device and methods
RU2713099C1 (ru) * 2019-07-15 2020-02-03 Александр Евгеньевич Кузнецов Устройство для обнаружения и распознавания аналитов в многокомпонентной среде и способ его изготовления
CN116660347A (zh) * 2023-05-15 2023-08-29 上海交通大学 一种仿生嗅觉传感器及其制备方法和应用

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