WO2011123776A3 - Method for detecting occult blood - Google Patents

Method for detecting occult blood Download PDF

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Publication number
WO2011123776A3
WO2011123776A3 PCT/US2011/030934 US2011030934W WO2011123776A3 WO 2011123776 A3 WO2011123776 A3 WO 2011123776A3 US 2011030934 W US2011030934 W US 2011030934W WO 2011123776 A3 WO2011123776 A3 WO 2011123776A3
Authority
WO
WIPO (PCT)
Prior art keywords
occult blood
chemifluorescent
chemiluminescent
specimen
testing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/030934
Other languages
French (fr)
Other versions
WO2011123776A2 (en
Inventor
B. Eugene Guthery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2011123776A2 publication Critical patent/WO2011123776A2/en
Publication of WO2011123776A3 publication Critical patent/WO2011123776A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/725—Haemoglobin using peroxidative activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A novel method for detecting occult blood in feces, urine, gastric juices and sputum is herein disclosed, representing an improvement in occult blood testing, particularly of the type which is readily useable by a lay person in a "self-testing" format. The method is suitable for application to a specimen predeposited in a testing zone, for example a toilet bowl. The need for specimen manipulation is eliminated. The method comprises application of an oxidizing agent preferably containing a metal chelator to the specimen of interest followed by application of a chemiluminescent or chemifluorescent compound. The ambient light is then eliminated allowing the chemiluminescent or chemifluorescent glow to be observed with the unaided eye. The chemiluminescent or chemifluorescent compounds of the invention are selected from purified sodium or potassium luminol, purified sodium or potassium benzyl (ghi) perylene hydrazide 1,2 dicarboxylic acid at a pH greater than 9.0. Using these sodium or potassium salts of hydrazides produces chemiluminescence with occult blood of such intensity that the results may be read with the unaided eye, thus eliminating the need for detection equipment.
PCT/US2011/030934 2010-04-01 2011-04-01 Method for detecting occult blood Ceased WO2011123776A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32003810P 2010-04-01 2010-04-01
US61/320,038 2010-04-01

Publications (2)

Publication Number Publication Date
WO2011123776A2 WO2011123776A2 (en) 2011-10-06
WO2011123776A3 true WO2011123776A3 (en) 2012-02-09

Family

ID=44712855

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/030934 Ceased WO2011123776A2 (en) 2010-04-01 2011-04-01 Method for detecting occult blood

Country Status (1)

Country Link
WO (1) WO2011123776A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102077288B1 (en) * 2011-11-30 2020-02-14 에릭 비. 휠든 Apparatus and method for the remote sensing of blood in human feces and urine
US8802442B2 (en) * 2011-11-30 2014-08-12 Eric B. Wheeldon Apparatus and method for the remote sensing of blood in human feces and urine
CN114624216A (en) * 2014-12-10 2022-06-14 丰能医药科技（上海）有限责任公司 A device for detecting occult blood in excreta
CA3231903A1 (en) 2015-02-25 2016-09-01 Outsense Diagnostics Ltd. Bodily emission analysis
CN107923907B (en) * 2015-08-28 2020-05-29 荣研化学株式会社 Reagent composition for immunological measurement and use thereof
US11111218B2 (en) * 2016-02-16 2021-09-07 Metriopharm Ag Crystalline form of 5-amino-2,3-dihydrophthalazine-1,4-dione
US11203575B2 (en) * 2016-02-16 2021-12-21 Metriopharm Ag Methhod for producing a crystalline form of 5-amino-2,3-dihydrophthalazine-1,4-dione
WO2018042431A1 (en) 2016-08-30 2018-03-08 Outsense Diagnostics Ltd. Bodily emission analysis
CN112858269A (en) * 2020-12-25 2021-05-28 广东绿康医疗器械有限公司 Preparation method and application method of detection reagent for nasopharyngeal mucosa cell free heme

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4578358A (en) * 1983-05-03 1986-03-25 Warner-Lambert Company Collection of specimens and detection of occult blood therein
US4725553A (en) * 1983-09-29 1988-02-16 Helena Laboratories Corporation Test composition for detecting occult blood
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US5840584A (en) * 1995-06-07 1998-11-24 Waldenburg; Ottfried Blood in feces test device
US20070037296A1 (en) * 2005-08-12 2007-02-15 Leslie Goulden Combined chemical and immunochemical fecal occult blood test

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4578358A (en) * 1983-05-03 1986-03-25 Warner-Lambert Company Collection of specimens and detection of occult blood therein
US4725553A (en) * 1983-09-29 1988-02-16 Helena Laboratories Corporation Test composition for detecting occult blood
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US5840584A (en) * 1995-06-07 1998-11-24 Waldenburg; Ottfried Blood in feces test device
US20070037296A1 (en) * 2005-08-12 2007-02-15 Leslie Goulden Combined chemical and immunochemical fecal occult blood test

Also Published As

Publication number Publication date
WO2011123776A2 (en) 2011-10-06

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