WO2012124890A3 - meso-2,3-부탄다이올의 제조방법 - Google Patents

meso-2,3-부탄다이올의 제조방법 Download PDF

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WO2012124890A3
WO2012124890A3 PCT/KR2012/000436 KR2012000436W WO2012124890A3 WO 2012124890 A3 WO2012124890 A3 WO 2012124890A3 KR 2012000436 W KR2012000436 W KR 2012000436W WO 2012124890 A3 WO2012124890 A3 WO 2012124890A3
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butanediol
meso
nucleotide
coding
present
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WO2012124890A2 (ko
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이진원
이수진
김보림
최우주
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Industry University Cooperation Foundation of Sogang University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01004—R,R-butanediol dehydrogenase (1.1.1.4)
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
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Abstract

본 발명은 meso-2,3-부탄다이올 제조방법에 관한 것이다. 보다 상세하게는, 다음의 뉴클레오티드 서열로 구성된 군으로부터 선택되는 최소 1종의 뉴클레오티드 서열을 포함하는 발현벡터로 형질전환된(cotransformed) meso-2,3-부탄다이올 과발현용 대장균를 제공한다: (a) 서열목록 제5서열에 기재된 아미노산 서열을 가지는 아세토인 환원효소(acetoin reductase)을 코딩하는 뉴클레오티드; (b) 아세토락테이트 디카복실레이즈(acetolactate decarboxylase)를 코딩하는 뉴클레오티드; 및 (c) 알코올 디하이드로지네이즈(alcohol dehydrogenase)를 코딩하는 뉴클레오티드. 본 발명의 형질전환 대장균은 야생종에서는 생산하지 못하는 2,3-부탄다이올을 생산할 수 있다. 따라서 당으로부터 meso-2,3-부탄다이올 생합성 경로 발현과 다른 종의 유전자 삽입에 의한 대사 흐름 변경을 통해 대량의 meso-2,3-부탄다이올 생합성이 가능하다.
PCT/KR2012/000436 2011-03-14 2012-01-18 meso-2,3-부탄다이올의 제조방법 Ceased WO2012124890A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0022359 2011-03-14
KR1020110022359A KR101334971B1 (ko) 2011-03-14 2011-03-14 비병원성 2,3-부탄다이올 생산을 위한 이종 대장균 및 제조방법

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WO2012124890A3 true WO2012124890A3 (ko) 2012-12-27

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Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013076144A2 (en) * 2011-11-21 2013-05-30 Metabolic Explorer Microorganism strains for the production of 2,3-butanediol
KR101534221B1 (ko) * 2013-06-04 2015-07-09 서강대학교산학협력단 (a) ｗａｂＧ 유전자가 불활성화 되어 있고 (b) ｂｕｄＲ 유전자가 과발현된 2,3-부탄다이올 생산용 재조합 균주
KR101590993B1 (ko) 2013-12-12 2016-02-02 서울대학교산학협력단 재조합 효모를 이용한 2,3-부탄다이올의 생산방법
ES2836746T3 (es) * 2014-10-21 2021-06-28 Composición que contiene meso-2,3-butanodiol
EP3378940B1 (en) 2015-10-27 2020-06-03 Korea Research Institute of Bioscience and Biotechnology Method for producing heavy chain diol
US10858661B2 (en) 2017-01-10 2020-12-08 University-Industry Cooperation Group Of Kyung Hee University Use of Methylomonas sp. DH-1 strain and its transformants
KR102028161B1 (ko) * 2017-01-10 2019-10-02 경희대학교 산학협력단 형질전환 미생물을 이용한 2,3-부탄디올 생산방법
CN108570440A (zh) * 2018-03-23 2018-09-25 山东大学深圳研究院 一株重组大肠杆菌及其在生产光学纯meso-2,3-丁二醇中的应用

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 2 August 2009 (2009-08-02), "Klebsiella oxytoca strain M5al alpha- acetolactate decarboxylase(budA) and alpha-actolactate synthase (budB) genes, complete cds; and acetoin (diacetyl) reductase (budC) gene, partial cds", accession no. Q253371 *
DATABASE GENBANK 6 January 2011 (2011-01-06), "Acetoin reductase [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]", accession no. P 002919837 *
DATABASE GENBANK 6 January 2011 (2011-01-06), "Acetolactate decarboxylase [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]", accession no. P 002919835 *
DAVID R. NIELSEN ET AL.: "Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli", BIOTECHNOLOGY JOURNAL., vol. 5, no. 3, March 2010 (2010-03-01), pages 274 - 284 *
KRISTINA BLOMQVIST ET AL.: "Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes", JOURNAL OF BACTERIOLOGY, vol. 175, no. 5, March 1993 (1993-03-01), pages 1392 - 1404 *
SADAHARU UI ET AL.: "Molecular generation of an Escherichia coli strain producing only the meso-isomer of 2,3-butanediol", JOURNAL OF FERMENTATION AND BIOENGINEERING., vol. 84, no. 3, 1997, pages 185 - 189 *
YAJUN YAN ET AL.: "Enantioselective synthesis of pure(R,R)-2,3-butane-diol in Escherichia coli with stereospecific secondary alcohol dehydro-genases", ORGANIC AND BIOMOLECULAR CHEMISTRY, vol. 7, no. 19, 2009, pages 3914 - 3917 *

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WO2012124890A2 (ko) 2012-09-20
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