WO2012141471A3 - 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법 - Google Patents

역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법 Download PDF

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WO2012141471A3
WO2012141471A3 PCT/KR2012/002696 KR2012002696W WO2012141471A3 WO 2012141471 A3 WO2012141471 A3 WO 2012141471A3 KR 2012002696 W KR2012002696 W KR 2012002696W WO 2012141471 A3 WO2012141471 A3 WO 2012141471A3
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induced pluripotent
pluripotent stem
stem cells
dedifferentiation
enhancer
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French (fr)
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WO2012141471A2 (ko
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황동연
이강인
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Industry Academic Cooperation Foundation of College of Medicine Pochon CHA University
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Industry Academic Cooperation Foundation of College of Medicine Pochon CHA University
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Publication of WO2012141471A3 publication Critical patent/WO2012141471A3/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 (a) 역분화 유도 인자를 코딩하는 유전자가 도입된 인간-유래의 체세포를, 프로틴 키나아제 C 저해제, 히스톤 데아세틸라제 저해제, 및 골 형성 단백질 경로 차단제로 이루어진 군으로부터 1종 이상 선택된 역분화-증진제을 포함하는 배지 중에서 배양하는 단계; 및 (b) 단계(a)로부터 얻어진 배양물로부터 배아줄기세포-유사 콜로니(embryonic stem cell-like colonies)를 분리하는 단계를 포함하는 역분화 만능 줄기세포(iPS cells)의 제조방법을 제공한다. 본 발명의 제조방법은 높은 효율로 역분화 만능 줄기세포를 제조할 수 있으며, 또한 무-이종감염물질(xenopathogen-free) 및 무-지지세포(feeder cell-free) 조건하에서 역분화 만능 줄기세포를 제조할 수 있다.
PCT/KR2012/002696 2011-04-12 2012-04-10 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법 Ceased WO2012141471A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110033789A KR101807704B1 (ko) 2011-04-12 2011-04-12 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법
KR10-2011-0033789 2011-04-12

Publications (2)

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WO2012141471A2 WO2012141471A2 (ko) 2012-10-18
WO2012141471A3 true WO2012141471A3 (ko) 2012-12-06

Family

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PCT/KR2012/002696 Ceased WO2012141471A2 (ko) 2011-04-12 2012-04-10 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법

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KR (1) KR101807704B1 (ko)
WO (1) WO2012141471A2 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101836523B1 (ko) 2012-10-18 2018-03-08 현대자동차주식회사 차량의 ｄｃｔ 제어방법
PL2970890T3 (pl) * 2013-03-14 2020-11-16 The Brigham And Women's Hospital, Inc. Kompozycje i sposoby ekspansji i hodowli nabłonkowych komórek macierzystych
WO2015178728A1 (ko) * 2014-05-23 2015-11-26 주식회사 비비에이치씨 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법
KR101699761B1 (ko) 2014-05-23 2017-01-25 주식회사 비비에이치씨 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법
EP3189134A1 (en) 2014-09-03 2017-07-12 The Brigham and Women's Hospital, Inc. Compositions, systems, and methods for generating inner ear hair cells for treatment of hearing loss
KR102413235B1 (ko) * 2017-12-22 2022-06-24 고려대학교 산학협력단 체세포에서 전능성 줄기세포로의 역분화 유도용 태반유래세포 조건화 배지 및 이를 이용한 역분화 유도 방법
KR102051221B1 (ko) * 2018-07-24 2019-12-03 한국과학기술원 Cfc 신드롬 환자의 발달 저해를 완화 할 수 있는 치료용 조성물
US11617745B2 (en) 2018-08-17 2023-04-04 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating FOXO
KR102131921B1 (ko) * 2018-10-30 2020-07-08 차의과학대학교 산학협력단 줄기 세포의 신경전구세포로의 분화용 조성물, 키트, 및 이를 이용한 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100173414A1 (en) * 2008-12-03 2010-07-08 International Stem Cell Corporation Methods of Deriving Differentiated Cells from Stem Cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100173414A1 (en) * 2008-12-03 2010-07-08 International Stem Cell Corporation Methods of Deriving Differentiated Cells from Stem Cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DUTTA ET AL.: "Self-renewal versus lineage commitment of embryonic stem cells: protein kinase C signaling shifts the balance", STEM CELLS, vol. 29, 3 February 2011 (2011-02-03), pages 618 - 628 *
HAO ET AL.: "Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells", PLOS ONE, vol. 3, no. IS.8, 6 August 2008 (2008-08-06), pages 1 - 8 *
HUANGFU ET AL.: "Induction of pluripotent stem stem cells by defined factors is greatly improved by small-molecule compounds", NATURE BIOTECHNOLOGY, vol. 26, no. 7, 22 June 2008 (2008-06-22), pages 795 - 797, XP002502536, DOI: doi:10.1038/NBT1418 *
ZHOU ET AL.: "High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors", STEM CELLS, vol. 28, 23 August 2010 (2010-08-23), pages 1741 - 1750 *
ZHU ET AL.: "Reprogramming of human primary somatic cells by Oct4 and chemical compounds", CELL STEM CELL, vol. 7, 3 December 2010 (2010-12-03), pages 651 - 655, XP055075780, DOI: doi:10.1016/j.stem.2010.11.015 *

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Publication number Publication date
WO2012141471A2 (ko) 2012-10-18
KR101807704B1 (ko) 2017-12-13
KR20120116193A (ko) 2012-10-22

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