WO2013105807A3 - 신규한 합성 조절 srna 및 그 제법 - Google Patents

신규한 합성 조절 srna 및 그 제법 Download PDF

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WO2013105807A3
WO2013105807A3 PCT/KR2013/000235 KR2013000235W WO2013105807A3 WO 2013105807 A3 WO2013105807 A3 WO 2013105807A3 KR 2013000235 W KR2013000235 W KR 2013000235W WO 2013105807 A3 WO2013105807 A3 WO 2013105807A3
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srna
synthesized
present
regulating
target gene
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WO2013105807A2 (ko
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이상엽
나도균
유승민
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Korea Advanced Institute of Science and Technology KAIST
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Korea Advanced Institute of Science and Technology KAIST
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Priority to CN201380012767.XA priority Critical patent/CN104254606B/zh
Priority to DK13735942.8T priority patent/DK2803727T3/en
Priority to ES13735942T priority patent/ES2709105T3/es
Priority to US14/371,557 priority patent/US9388417B2/en
Priority to EP13735942.8A priority patent/EP2803727B1/en
Publication of WO2013105807A2 publication Critical patent/WO2013105807A2/ko
Publication of WO2013105807A3 publication Critical patent/WO2013105807A3/ko
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Abstract

본 발명은 신규 구조의 원핵세포 내에서 유전자 발현을 감소시키는 맞춤형 합성 sRNA, 그 제법 및 그 용도에 관한 것으로, 보다 상세하게는 MicC, SgrS 및 MicF 중 어느 하나의 sRNA 유래의 Hfq 결합 부위(Hfq binding site) 및 표적 유전자 mRNA와 상보적 결합을 형성하는 영역을 포함하는 합성 sRNA, 그 제법 및 그 용도에 관한 것이다. 본 발명에 따른 합성 sRNA는 표적 유전자의 mRNA와의 결합력을 조절함으로써 억제 정도를 조절할 수 있는 장점이 있으며, 유전자 발현을 조절하는 합성 sRNA를 통해 기존의 유전자 결실을 통한 과정 없이 효과적으로 제작, 목적 유전자 발현을 감소시킬 수 있어 재조합 미생물 제조에 유용하며, 다양한 균주에서 빠르게 적용할 수 있어 균주별 대사 능력 측정 및 최적 균주 선정에 매우 적합하다. 아울러, 합성 sRNA를 통한 미생물 대사 흐름 조작에 의해 수득된 본 발명에 따른 tyrosine 혹은 cadaverine을 고효율로 생산하는 재조합 미생물은 의약품 및 산업용제 미생물로 유용하다. 즉, 본 발명에 따른 sRNA를 이용하여 대사산물의 고효율 생산을 위한 발현 억제 타겟 유전자 선별을 용이하게 할 수 있다. 이에 다양한 대사 산물의 효율적 생산을 위한 재조합 균주의 제작 및 효율적 생산방법 확립에 사용할 수 있는바 매우 유용하다.
PCT/KR2013/000235 2012-01-11 2013-01-11 신규한 합성 조절 srna 및 그 제법 Ceased WO2013105807A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN201380012767.XA CN104254606B (zh) 2012-01-11 2013-01-11 新型合成-调控sRNA及其制备方法
DK13735942.8T DK2803727T3 (en) 2012-01-11 2013-01-11 SYNTHESIS REGULATORY SRNA AND METHOD OF PRODUCING THEREOF
ES13735942T ES2709105T3 (es) 2012-01-11 2013-01-11 ARNs de regulación de síntesis y procedimiento de preparación del mismo
US14/371,557 US9388417B2 (en) 2012-01-11 2013-01-11 Synthesis-regulating sRNA and method for preparing same
EP13735942.8A EP2803727B1 (en) 2012-01-11 2013-01-11 Synthesis-regulating srna and method for preparing same

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Application Number Priority Date Filing Date Title
KR20120003609 2012-01-11
KR10-2012-0003609 2012-01-11

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WO2013105807A2 WO2013105807A2 (ko) 2013-07-18
WO2013105807A3 true WO2013105807A3 (ko) 2013-10-10

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US (1) US9388417B2 (ko)
EP (1) EP2803727B1 (ko)
KR (1) KR101575587B1 (ko)
CN (1) CN104254606B (ko)
DK (1) DK2803727T3 (ko)
ES (1) ES2709105T3 (ko)
WO (1) WO2013105807A2 (ko)

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CN113817732B (zh) * 2021-08-18 2023-12-01 中国农业科学院生物技术研究所 具有固氮基因沉默功能的人工非编码rna及其应用
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KR102853096B1 (ko) * 2022-03-18 2025-09-02 씨제이제일제당 주식회사 카르바모일 인산 합성효소의 활성이 약화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법
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KR101575587B1 (ko) 2015-12-09
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