WO2014018751A1 - Dispositif de filtration de cellules - Google Patents

Dispositif de filtration de cellules Download PDF

Info

Publication number
WO2014018751A1
WO2014018751A1 PCT/US2013/052052 US2013052052W WO2014018751A1 WO 2014018751 A1 WO2014018751 A1 WO 2014018751A1 US 2013052052 W US2013052052 W US 2013052052W WO 2014018751 A1 WO2014018751 A1 WO 2014018751A1
Authority
WO
WIPO (PCT)
Prior art keywords
section
well
disposed
filtration device
cell filtration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/052052
Other languages
English (en)
Inventor
John Donald ROBACK
Wenhong Neoh
Kaitlyn Frazier STRICKLAND
Michael J. WEILER
Jessica KANTER
Justin GO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Emory University
Georgia Tech Research Institute
Georgia Tech Research Corp
Original Assignee
Emory University
Georgia Tech Research Institute
Georgia Tech Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emory University, Georgia Tech Research Institute, Georgia Tech Research Corp filed Critical Emory University
Publication of WO2014018751A1 publication Critical patent/WO2014018751A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components

Definitions

  • the disclosure relates to devices configured for use with automated testing or analyzing instruments.
  • the devices are configured to eliminate expensive steps and/or components required by many automated testing instruments.
  • the devices can be configured for testing of a sample, for example, blood typing of a RBC sample.
  • the devices may include a well member configured to be disposed in a sample well of a well plate.
  • the well member may include a first end and an opposing second end.
  • the well member may further include a first section disposed at the first end and a second section disposed at the second end.
  • the second section may define a reservoir configured to hold a predetermined volume of a fluid.
  • the well member may include a plurality of cut-outs that extend between the first section and the second section.
  • the devices may include a plurality of support members extending between the first section and the second section, each cut-out being disposed between a set of support members.
  • the devices may include a filter member disposed to surround the well member, the filter member at least covering the cut-out members.
  • the support members may be configured to support the filter member.
  • the filter member may include a hydrophilic material.
  • the filter member may have a pore size of less than about 2.0 ⁇ .
  • the absorbent member may be disposed to surround the filter member and configured to absorb some of the fluid. In some embodiments, the absorbent member may be configured to be disposed adjacent to a wall of the sample well.
  • the devices may include a depression configured to receive the well member.
  • the depression may be configured to be disposed within the sample well.
  • the first section has a length and the second section has a length and the length of the second section may be longer than the length of the first section.
  • the length of the second section may correspond to the predetermined volume.
  • the devices may include a well member configured to be disposed in a sample well of a well plate.
  • the well member may include a first end and an opposing second end.
  • the well member may include a first section disposed at the first end and a second section disposed at the second end, the second section defining a reservoir configured to hold a predetermined volume of a fluid; a plurality of support members extending between the first section and the second section; and plurality of cut-outs extending between the first section and the second section and disposed between a set of plurality of support members.
  • the cut-outs may be configured to allow the fluid to flow out of the well member.
  • the devices may include a filter member disposed to at least partially surround the well member. The filter member may at least cover the cut-out members and may be configured to substantially contain the sample within the well member during the testing.
  • the devices may include a plurality of support members extending between the first section and the second section. Each cut-out may be disposed between a set of support members.
  • the support members may be configured to support the filter member.
  • the filter member may include a hydrophilic material. In some embodiments, the filter member may have a pore size less than 2.0 ⁇ .
  • the devices may include a well plate.
  • the well plate may include a plurality of sample wells.
  • the devices may include a depression disposed on the bottom surface of the sample well and configured to receive the well member.
  • the devices may include an absorbent member that surrounds the filter member and is disposed between the filter member and a wall of the sample well. The absorbent member may be configured to absorb at least some of the fluid.
  • the absorbent member may include a material having hydrophilic properties.
  • the second section may be configured to control a volume of the fluid for the testing.
  • Figure 1 shows a cell filtration device according to embodiments
  • Figure 2 shows an enlarged view of the cell filtration device in Figure 1 ;
  • Figure 3 shows an enlarged perspective view of the cell filtration member according to embodiments
  • Figure 4 shows an enlarged front view of the cell filtration member
  • Figure 5 shows an enlarged top view of the cell filtration member
  • Figure 6 shows prototypes of the cell filtration member
  • Figure 7 shows an orthographic illustration of the cell filtration member
  • Figure 8 shows an exploded view of the cell filtration member
  • Figure 9 shows a top perspective view of the well plate according to
  • Figure 10 shows a bottom perspective view of the well plate according to
  • Figure 11 shows a method of using the cell filtration device in a blood-typing assay procedure.
  • the cell filtration devices can address the deficiencies of the current automated systems by being configured to eliminate expensive steps and/or components.
  • the cell filtration devices are designed to remove excess fluid from a cell samples (e.g., red blood cells (RBC)).
  • RBC red blood cells
  • the cell filtration device can be capable of isolating cells in solution without inflicting the cell damage associated with the vacuum or centrifuge.
  • the devices can rely upon passive fluid diffusion and hydrophilic forces to achieve fluid filtration rather than a vacuum gradient or gravitational separation.
  • centrifugation can be no longer required.
  • the cell filtration devices may be used with any blood typing system.
  • the embodiments are described with respect to RBC samples and blood typing tests.
  • the cell filtration device may be used with other cell samples and other tests.
  • the cell filtration devices may be used with the corresponding system for that test.
  • the dimensions of the well member (also referred to as “filter well insert” or “inner well”) and/or well plate (also referred to as “outer well” or “main well”) may be adjusted to be compatible with the system.
  • Figures 1-10 show a cell filtration device according to embodiments.
  • the cell filtration device 100 may include a well member 110 configured to be disposed within a sample well of a well plate (see, for example Figures 1, 2 and 8).
  • the well member 110 may be configured to hold a sample (e.g., a RBC sample) and a fluid (e.g., assay wash fluid).
  • Figures 3-5 show the well member 110 according to embodiments.
  • the well member 110 may be configured to contain the sample for the duration of the assay process.
  • the well member 110 may have a substantially cylindrical shape. In other embodiments, the well member 110 may have a different shape. The shape may depend on the wells of the well plate.
  • the well member 110 may be a substantially hollow structure.
  • the well member 110 may be made of a polymer material.
  • the material may be biocompatible.
  • the well member 110 may be made of polystyrene, for example, through plastic injection molding.
  • the well member 110 may have a first end 210 and a second end 220 (also referred to as "lower” or “bottom") that opposes the first end 210.
  • the first end 210 may be an open end and define an opening 212.
  • the second end 220 may be a substantially closed end and/or an open end.
  • the second end 220 may be entirely or partially closed.
  • the second end 220 may define a closed end with a bottom of a sample well of a well plate or a molded insert.
  • the first well member 110 may have a length 202 that extends between the first end 210 and the second end 220.
  • the well member 110 may include a body portion 230 that extends along the length 202.
  • the body portion 230 may include a bore 232 that extends substantially along the length 202.
  • the bore 232 may terminate at the second end 220.
  • the well member 110 may have a wall thickness 204. In some embodiments, the well member 110 may have the same wall thickness. In some embodiments, the wall thickness 204 may be about 0.0050".
  • the well member 110 may have a diameter 206 (also referred to as the "outer diameter") that is the same along the length 202.
  • the diameter 206 may be about 0.367 inches.
  • the well member 110 may have a different diameter along the length 202, for example, the well member 110 may be tapered.
  • the opening 212 and the bore 232 may have a diameter 208 that is the same (also referred to as the "inner diameter" of the well member 110). In some embodiments, the diameter 208 may be about 0.267". In other embodiments, a diameter of the opening 212 and the diameter of the bore 232 may be the different.
  • the well member 110 may include a (bottom) surface 222 that extends across the bore 232 and is disposed at the second end 220 (perpendicular to the length 202).
  • the surface 222 may define the closed end.
  • the well member 110 may omit a bottom surface 222.
  • the closed end may be defined by the second end 220 and the bottom of a sample well and/or a bottom of a molded insert.
  • the well member 110 may include a member configured to provide a seal between the bottom of the sample well and the well member 110.
  • the member may be disposed at the second end and substantially surround the bore 232.
  • the member may be a silicone ring or the like.
  • the well member 110 may include a first section 240 disposed at the first end 210.
  • the first section 240 may be disposed to surround the opening 212 and the bore 232.
  • the first section 210 may extend parallel to the length 202 of the well member 110.
  • the well member 110 may include a second section 250 also referred to as "lower lip” and "bottom lip") disposed at the second end 220.
  • the second section 250 may be configured to hold a predetermined volume of a solution or a fluid (e.g., a RBC solution).
  • the second section 250 may be disposed to surround the bore
  • the second section 250 may extend parallel to the length 202 of the well member 110 from the second end 220.
  • the second section 250 and the bottom surface 222 may define a reservoir 256 (also referred to as "well depression") configured to hold a predetermined volume of a solution (e.g., a RBC solution).
  • the second section 250 may be configured to hold any volume of a solution.
  • the length 252 of the second section 250 may determine the capacity of the second section 250.
  • the dimensions (e.g., the length 252 and/or diameter) of the second section 250 may be adjusted to meet the fluid volume needs of any particular application.
  • the second section may be configured to hold a volume of about 100 ⁇ , a volume of about 187 ⁇ ⁇ , and the like.
  • the second section 250 may be configured to control the final fluid volume after the assay process. This can improve operation as the flow cytometer is concentration dependent.
  • the dimensions (e.g., length) of the second section 250 may be adjusted to meet the fluid volume needs of any particular application.
  • the well member 110 thus can be easily adaptable to ancillary tests.
  • Figure 6 shows prototypes 610 and 620 of a well member according embodiments with different sized reservoirs.
  • the prototypes 610 and 620 are substantially identical but for the different sized second sections 612 and 622, respectively.
  • the second section 612 of the well member 610 is bigger (e.g., longer length) than the second section 622 of the well member 620.
  • the well member 610 has the second section 612 configured for a volume of about 187 ⁇ .
  • the well member 620 has the second section 622 configured for a volume of about ⁇ ⁇ .
  • first section 240 and the second section 250 may have the same diameter. In other embodiments, the first section 240 and the second section 250 may have different diameters.
  • the first section 240 may be smaller than the second section
  • the first section 240 may have a length 242 that is smaller than a length 252 of the second portion of the second section 250. In other embodiments, the first section 240 may be larger than the second section 250. The length 242 of the first section 240 may be larger than the length 252 of the second section 250. In further embodiments, the first section 240 may have substantially the same dimensions of the second section 250. The length 242 of first section 240 may be the same as the length 252 of the second section 250.
  • the well member 110 may include a plurality of support members 260 (also referred to as “vertical supports") extending partially along the length 202 between the first section 240 and the second section 250.
  • the support members 260 may be disposed around and surround the bore 232.
  • the well member 110 may include a plurality of cut-outs 270 disposed at least partially along the length 202 between the first section 240 and the second section 250.
  • the cut-outs 270 may be disposed around and surround the bore 232. The width or
  • each cut-out 270 may be defined by two opposing support members 260.
  • the cut-outs 270 may be configured to allow a fluid (e.g., a washing fluid) to flow out of the (bore 232) of the well member 110.
  • a fluid e.g., a washing fluid
  • the well member 110 may include four support members 260 and four cut-outs 270, as shown in the figures. In other embodiments, the well member 110 may include a different number of the support members 260 and the cut-outs 270. For example, the well member 110 may include two, three, five, six, etc. number of the support members 260 and cut-outs 270. The number of the plurality of support members 260 may correspond to the number of cut-outs 270.
  • the well member 110 may have any combination of sizes of the support members
  • each cut-out 270 may have a length 274 of about 0.052 inches. However, the length 274 of the cut-outs 270 is not limited to this length and may be of a different length.
  • the cut-outs 270 may have a width (or circumference) 272 that is substantially the same. In this way, the spacing between the support members may be substantially the same. In some embodiments, the cut-outs 270 may be of different sizes. For example, the cutouts 270 may have varying and different widths. The cut-outs 270 may have any combination of widths. In this way, the spacing between the supports members 260 may vary and be different.
  • the support members 260 may be substantially the same. In other embodiments, the support members 260 may be different and vary in size. For example, the support members may have varying width.
  • the support members 260 and the cut-outs 270 may have the same dimensions. In other embodiments, the support members 260 and the cut-outs 270 may have different dimensions. For example, the support members 260 may have a different width or circumference than the cut-outs 270. The width or circumference of the support members may be larger or smaller than the width or the circumference of the cut-outs 270.
  • the cut-outs 270 may be disposed relative to the body 230 at predetermined angle 276.
  • the angle 276 may be about 65°. In other embodiments, the predetermined angle 276 may be different.
  • the cell filtration device 100 may include a filter member 120 disposed to at least partially surround the well member 110.
  • the filter member 110 may be configured to substantially contain the sample (e.g., RBCs) within the well member 110 for the duration of the assay process.
  • the filter member 120 may be fixedly disposed to the outer diameter of the well member 110, for example, by an adhesive.
  • the filter member 120 may have at least a length that covers at least the cut-outs 270.
  • the support members 260 of the well member 110 may be configured to support the filter member 120 in addition to supporting the body of the well member 110.
  • the filter member 120 may be made of a semi-permeable material.
  • the material may be hydrophilic.
  • the hydrophilic material may include but is not limited to a polyester material.
  • the filter member 120 may include at least one layer of a hydrophilic material.
  • the filter member 120 may be configured to draw an assay fluid out of the well member 110 at a desired diffusion rate. For example, a filter member having a diffusion rate of about 20 seconds may have a filter size of about ⁇ . ⁇ pore size.
  • the filter member 120 may have a pore size configured to prevent passage of the sample (e.g., RBC cells) to be analyzed across the filter member 120.
  • the pore size may depend on the sample to be analyzed. It can be adjusted to meet the individual pore size requirements of the assay applications.
  • the pore size may be generally the largest pore size that can prevent the passage of the cells across the filter member 120, and thus the filter member 120 may be configured to allow the fastest diffusion without risk of cell sample loss.
  • RBCs generally can be as small as 2.0 ⁇ .
  • the pore size of the filter member 120 may be less than about 2.0 ⁇ .
  • the filter member 120 may include a pore size of about 1.5 ⁇ or 1.0 ⁇ .
  • the filter member 120 may have any thickness and any pore density.
  • the filter member 120 may have a thickness of about 11 ⁇ and a pore density of about 2 x 10 7 pores/cm 2 .
  • the cell filtration device 100 may include an absorbent member 130 disposed to substantially surround the filter member 120.
  • the absorbent member 130 may be configured to absorb at least some of the fluid and draw the assay wash from the well member 110 through the filter member 120.
  • the absorbent member 130 may be made of any material(s) having hydrophilic properties.
  • the absorbent material may include but is not limited to cotton materials, silicia gel derivatives, and the like.
  • the absorbent material may be configured to produce an average diffusion rate and absorb enough fluid to withstand a number of washes required for the application without requiring a change of material.
  • the material may be a highly absorbent cotton that is configured to produce average diffusion rates of less than about 20 seconds and configured to withstand about six washes.
  • the absorbent member 130 may be bonded to the filter member 120 together during the manufacturing process of the members, for example, by needle- punching. In other embodiments, the absorbent member 130 and the filter member 120 may be separately manufactured. The absorbent member 130 may be disposed to surround the filter member 120 during the manufacturing of the cell filtration device 100.
  • the cell filtration device 100 may include a well plate 140
  • the well plate 140 may be any microplate or microwell plate.
  • Figures 9 and 10 show the well plate 140 according to embodiments. It will be understood that the well plate 140 is not limited to the well plate shown in and described with respect to the figures and may be any microplate or microwell plate.
  • the well plate 140 may be made of the same material or a different material as the cell filtration member 110.
  • the well plate 140 may be made of a polymer material having biocompatibility. In some embodiments, the material may include but is not limited to polystyrene.
  • the well plate 140 may be manufactured using injection-molding methods.
  • the well plate 140 may include a plurality of sample wells 902.
  • the well plate 140 may include any number of sample wells 902 and is not limited to the 24 sample wells shown.
  • the well plate 140 may include 6, 24, 96, 384, or 1536 sample wells arranged in a rectangular matrix.
  • the well plate 140 may be configured to compatible with existing pipette and flow cytometry equipment.
  • the dimensions of the well plate 140 may correspond to a standard microtiter plate. The dimensions may alternatively or optionally correspond to the requirements in the
  • the well plate 140 may have a length by width of about 5.023" by about 3.369".
  • the inner diameter of each sample well 902 may be about 0.27" and the height of each sample well may be about 0.67".
  • the well plate 140 may include a first section 910 and a second section 920.
  • the first section 910 may include the plurality of sample wells 902.
  • each of the sample wells 902 may include the well member 110, the filter member 120 and the absorbent member 130.
  • the second section 920 may include a planar surface on which the first section 910 is disposed.
  • the second section 920 may have a longer width and diameter than the first section 910 so as to define a "flange.”
  • the "flange" may be considered the area of the second section 920 that extends beyond the first section 910.
  • the second section 920 may include a top surface 922 on which the first section 910 is disposed and an opposing bottom surface 924.
  • the well plate 140 may include least one protrusion 930 disposed on the bottom surface 924.
  • the well plate 140 may include any number of protrusions 930 and is not limited to the ten protrusions shown in Figure 10.
  • the well plate 140 may include a plurality of the protrusions 930, such as more or less than the ten protrusions shown.
  • the protrusions 930 may be configured to enable stacking of the plates 140.
  • the protrusions 930 may be disposed within the flange (the area that extends beyond the first section 910) on the bottom surface 930.
  • the well plate 140 may include at least one angled surface 940
  • the first section 910 may include at least one angled surface 940 disposed at one of the corners.
  • the first section 910 may include two angled surfaces 940 disposed at opposing corners on the same side.
  • the (top) surface 912 of the first section 910 of the well plate is the (top) surface 912 of the first section 910 of the well plate
  • the 140 may include an inlet 950 configured to provide protection to the well members 140.
  • the inlet 950 may have any dimension and may include and is not limited to 1/32".
  • the inlet 950 may be configured to provide protection, for example, during shipping, storage, handling, and usage in the lab environment.
  • the cell filtration device 100 may include a depression 150 configured to receive the well member 110.
  • the depression 150 may be configured to be disposed in a sample well of a well plate.
  • the depression 150 may be configured to be disposed at the bottom of a sample well.
  • the inner diameter of the depression 150 may correspond to the (outer) diameter of the well member 110.
  • the depression 150 may have dimensions of about 0.400" (diameter) by about 0.0625" (height).
  • the well plate 140 may include the depression 150.
  • Each sample well of the well plate 140 may include the depression 150.
  • a sample well of the well plate 140 may be retrofitted with the depression 150.
  • Figure 7 shows the well plate 140 including the depression 150.
  • the depression 150 may be made of a polymer material.
  • the depression 150 may be made of the same and/or different material as the cell filter member 110 and the well plate 140.
  • the depression 150 may be made of polystyrene.
  • the cell filtration device 100 may alternatively or additionally
  • the depression 150 includes a member configured to provide a seal between the well member 100 and the well plate 140.
  • the member may be disposed at the second end and/or at the bottom of a sample well.
  • the member may be a silicone o-ring or the like.
  • the cell filtration device 100 may include the well plate 140.
  • each sample well may be configured as shown in Figures 1, 2, 7, and 8. As shown in these figures, each sample well may include the well member 110 disposed within the depression 150 provided on the bottom of the sample well of the plate 140.
  • the filter member 120 may surround the well member 110 so as to at least partially cover the cut-outs.
  • the absorbent member 130 may be disposed or be configured to be disposed adjacent to a wall 142 of the sample well and surround the filter member 120.
  • At least the well plate 140 and the cell filter member 110 may be manufactured together as part of a singular mold.
  • the cell filtration device 100 may not include a well plate 140.
  • the cell filtration device may be configured to retrofit an existing well plate.
  • the cell filtration device may be an insert configured to fit into an existing plate.
  • the cell filtration device may include the cell filtration member, the filtration member and the absorbent member according to embodiments.
  • the cell filtration device may include the depression.
  • the cell filtration device 100 may include a molded insert including a plurality of protrusions substantially corresponding to the sample wells of a well plate. The insert maybe configured to be disposed on a top surface of a well plate and the protrusions may be configured to be disposed within the corresponding sample well. The protrusions may have a substantially open or closed bottom.
  • the molded insert may include a depression according to embodiments.
  • the depression may be disposed on the bottom of each protrusion.
  • the bottom surface of the protrusion may be the depression and/or the depression may be disposed on top of or above the bottom surface of the protrusion.
  • the insert may additionally or alternatively (to the depression) include a member disposed on the bottom of each protrusion.
  • the member may be configured to provide a seal between the bottom of the sample well and the bottom of the insert.
  • the member may be a silicone o-ring or the like.
  • the bottom of the protrusion with the second section of the well member may be configured to form a closed end and a reservoir. In other embodiments, the bottom of the protrusion and the bottom of the sample well may be configured to form a closed end and a reservoir.
  • the cell filtration member, the filtration member and the absorbent member may be disposed within each protrusion as shown in and described with respect to Figures 7 and 8 and the sample well of a well plate.
  • the cell filtration member may be configured to be sterilized or cleaned. In some embodiments, one, some, or all parts of the cell filtration member may be configured to be reused. In further embodiments, one, some, or all parts of the cell filtration member may be disposable. In further embodiments, the cell filtration member may be a single, use device.
  • the cell filtration device may be part of a kit.
  • the cell filtration device may include any combination of the well member, the filter member, the absorbent member, the depression.
  • the cell filtration device may also include the well plate.
  • the cell filtration device may include a molded insert for a well plate.
  • the kit may include a plurality of the cell filtration devices.
  • the cell filtration devices are configured to substantially remove the assay wash fluid from the cell sample and configured to contain the cell sample within a specified chamber (e.g., the bore within the well member). More specifically, the cell filtration devices are configured to leverage the difference in hydrophilicity between the cell filtration member and the well plate to create a strong enough driving force to encourage passive fluid diffusion, i.e., pull the fluid from the well member to the outer hydrophilic member. In this way, the cell filtration devices can isolate the cells (e.g., RBCs) in solution so that they can be feed into a flow cytometer to determine blood type.
  • the cell filtration devices according to embodiments can thereby allow for the streamlining of manufacturing and material costs, drastic reduction in overall testing times and easy integration into compact systems, such as the AEGIS platform manufactured by 3Ti.
  • Figure 11 shows a method 1100 of performing a blood-typing method using the cell filtration devices and related operation of the cell filtration devices according to embodiments.
  • the method may be performed using a system that includes at least pipette and flow cytometry equipment and may be performed using automated equipment.
  • the method does not require a centrifuge or a vacuum device.
  • the reaction steps can begin. First, a hemocrit solution (e.g., a 1.5% hemorcrit solution) and reagents may be added (steps 1110 and 1120, respectively). Next, the wash steps may begin. A saline solution (e.g., 200 ⁇ ⁇ of a saline a solution) may be added to the cell filtration member (step 1130). The absorbent member may then absorb some of the saline solution (step 1140), thereby wicking or absorbing the excess assay fluid out of the cell filtration member. After the excess assay fluid 1152 is absorbed (step 1150), the sample 1102 may be analyzed (step 1160).
  • a hemocrit solution e.g., a 1.5% hemorcrit solution
  • reagents e.g., a 1.5% hemorcrit solution
  • the wash steps may begin.
  • a saline solution e.g., 200 ⁇ ⁇ of a saline a solution
  • the absorbent member may then absorb
  • the remaining fluid (e.g., 20 ⁇ .) of the final suspension can be withdrawn and analyzed by a flow cytometer.
  • the second section e.g., reservoir
  • the second section is thereby configured to prevent the absorbent member from contacting the remaining assay fluid, thus allowing precision control of the remaining fluid volume to be sent to the flow cytometer for typing.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne des dispositifs, conçus à partir des déficiences des systèmes automatiques actuels d'analyse d'échantillons de cellules, et destinés à éliminer les étapes et/ou composants coûteux. Les dispositifs peuvent être capables d'isoler les cellules dans une solution, sans infliger aux cellules de dégâts associés à la mise sous vide ou à la centrifugation. Les dispositifs peuvent comprendre au moins un élément formant puits, conçu pour être placé dans un puits d'échantillon d'une plaque de puits. L'élément formant puits peut comprendre une première extrémité et une seconde extrémité opposée ; une première section disposée sur la première extrémité et une seconde section disposée sur la seconde extrémité, la seconde section définissant un réservoir conçu pour contenir un volume prédéfini d'un fluide ; et une pluralité de découpes, s'étendant entre la première section et la seconde section, les découpes étant conçues pour permettre au fluide de sortir de l'élément formant puits.
PCT/US2013/052052 2012-07-27 2013-07-25 Dispositif de filtration de cellules Ceased WO2014018751A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261676357P 2012-07-27 2012-07-27
US61/676,357 2012-07-27

Publications (1)

Publication Number Publication Date
WO2014018751A1 true WO2014018751A1 (fr) 2014-01-30

Family

ID=49997835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/052052 Ceased WO2014018751A1 (fr) 2012-07-27 2013-07-25 Dispositif de filtration de cellules

Country Status (1)

Country Link
WO (1) WO2014018751A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3834938A1 (fr) * 2019-12-10 2021-06-16 Tecan Trading Ag Insert pour un réseau de puits, procédé d'application et d'utilisation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124715A1 (en) * 1999-07-16 2003-07-03 Tortorella Micky D. Novel filtrate plate device and reversible-well plate device
US6830732B1 (en) * 2000-08-02 2004-12-14 Invitek Gmbh Multiwell filtration plate
US20080003670A1 (en) * 2006-06-30 2008-01-03 Corning Incorporated High density permeable supports for high throughput screening
US20110165589A1 (en) * 2008-05-14 2011-07-07 Fabpulous B.V. Device and method for separating and analyzing blood
JP2011189270A (ja) * 2010-03-15 2011-09-29 Sumitomo Bakelite Co Ltd マイクロプレートおよび分離方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124715A1 (en) * 1999-07-16 2003-07-03 Tortorella Micky D. Novel filtrate plate device and reversible-well plate device
US6830732B1 (en) * 2000-08-02 2004-12-14 Invitek Gmbh Multiwell filtration plate
US20080003670A1 (en) * 2006-06-30 2008-01-03 Corning Incorporated High density permeable supports for high throughput screening
US20110165589A1 (en) * 2008-05-14 2011-07-07 Fabpulous B.V. Device and method for separating and analyzing blood
JP2011189270A (ja) * 2010-03-15 2011-09-29 Sumitomo Bakelite Co Ltd マイクロプレートおよび分離方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3834938A1 (fr) * 2019-12-10 2021-06-16 Tecan Trading Ag Insert pour un réseau de puits, procédé d'application et d'utilisation

Similar Documents

Publication Publication Date Title
US8404489B2 (en) Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus
EP3218300B1 (fr) Plateforme microfluidique pour l'étude d'interactions à base de cellules
CA2785390C (fr) Systeme et procede pour filtration de particules
JP2017200481A (ja) 試料導入から結果出力までのプロセス化を提供する単一構造バイオチップおよび製造方法
TWI789746B (zh) 液態檢體的自動化處理裝置
WO2008127639A1 (fr) Système de fractionnement de suspension à flotteur
CN107107059A (zh) 具有移液引导件的微流体芯筒
US9926553B2 (en) Sample extraction and preparation device
EP3395939B1 (fr) Cuve de tri, de culture et de croissance cellulaires ; et procédé de tri, de culture et de croissance cellulaires
EP3349897B1 (fr) Dispositif et procede de separation de liquides par centrifugation en dependance de leur densité
JP2014532874A (ja) ２次的液体を使って懸濁液の成分を分離するための方法およびシステム
US9816903B2 (en) Filtration device for liquid samples
WO2020039677A1 (fr) Dispositif de filtration et procédé de filtration
WO2014018751A1 (fr) Dispositif de filtration de cellules
CN115926980A (zh) 一种用于细胞培养的芯片装置及方法
KR101955330B1 (ko) 핵산 분석용 반응 시약 공급 장치
CN111044514A (zh) 一种富集方法、样本制造方法以及样本检测方法
KR101573501B1 (ko) 세포 분리칩 및 이를 이용한 세포 분리 방법
EP4050336B1 (fr) Enrichisseur, système d'enrichissement, système de fabrication d'échantillon et système de détection d'échantillon
CN211856600U (zh) 一种富集器、富集系统、样本制作系统和样本检测系统
EP4031286B1 (fr) Pot jetable de centrifugeuse
US20110178286A1 (en) Consumable analytical plasticware comprising high-solubility plastics
WO2008134748A1 (fr) Couvercle de microplaque à dessus maillé et ensemble
WO2021023722A1 (fr) Couvercle de récipient de fluide
Asai Akashi et a

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13823631

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13823631

Country of ref document: EP

Kind code of ref document: A1