WO2014100879A3 - Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases - Google Patents

Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases Download PDF

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Publication number
WO2014100879A3
WO2014100879A3 PCT/BR2013/000608 BR2013000608W WO2014100879A3 WO 2014100879 A3 WO2014100879 A3 WO 2014100879A3 BR 2013000608 W BR2013000608 W BR 2013000608W WO 2014100879 A3 WO2014100879 A3 WO 2014100879A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
cotton plants
chitin synthase
gene silencing
insect pests
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2013/000608
Other languages
English (en)
French (fr)
Other versions
WO2014100879A8 (pt
WO2014100879A2 (pt
Inventor
Maria Fátima GROSSI DE SÁ
Maria Cristina Mattar Da Silva
Leonardo LIMA PEPINO DE MACEDO
Alexandre Augusto PEREIRA FIRMINO
Roberta RAMOS COELHO
Isabela TRISTAN LOURENÇO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Original Assignee
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA filed Critical Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Priority to US14/653,699 priority Critical patent/US10182571B2/en
Publication of WO2014100879A2 publication Critical patent/WO2014100879A2/pt
Publication of WO2014100879A8 publication Critical patent/WO2014100879A8/pt
Publication of WO2014100879A3 publication Critical patent/WO2014100879A3/pt
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Insects & Arthropods (AREA)
  • Food Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A presente invenção está relacionada ao controle de infestação de praga através da inibição ou redução da expressão de genes da família da quitina sintase. A invenção provê ainda métodos e composições para o controle de pragas, através da alimentação de uma ou mais moléculas de RNA de fita dupla provida pela presente invenção. A invenção descreve ainda um método de obtenção de plantas transgênicas que expressem moléculas de RNA de fita dupla. A presente invenção é preferencialmente utilizada para plantas de algodoeiro.
PCT/BR2013/000608 2012-12-28 2013-12-27 Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases Ceased WO2014100879A2 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/653,699 US10182571B2 (en) 2012-12-28 2013-12-27 Method and compositions for the genetic control of insect pests in cotton plants by chitin synthase gene silencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRBR1020120335395 2012-12-28
BR102012033539A BR102012033539A8 (pt) 2012-12-28 2012-12-28 método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases

Publications (3)

Publication Number Publication Date
WO2014100879A2 WO2014100879A2 (pt) 2014-07-03
WO2014100879A8 WO2014100879A8 (pt) 2014-09-04
WO2014100879A3 true WO2014100879A3 (pt) 2014-11-06

Family

ID=51022142

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2013/000608 Ceased WO2014100879A2 (pt) 2012-12-28 2013-12-27 Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases

Country Status (4)

Country Link
US (1) US10182571B2 (pt)
AR (1) AR094337A1 (pt)
BR (1) BR102012033539A8 (pt)
WO (1) WO2014100879A2 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017074780A2 (en) * 2015-10-28 2017-05-04 Dow Agrosciences Llc Nucleic acid molecules that confer resistance to coleopteran pests
BR112020020870A2 (pt) 2018-07-03 2021-04-06 Embrapa-Empresa Brasileira De Pesquisa Agropecuaria Pesticida
CN119874868A (zh) * 2025-01-13 2025-04-25 贵州省烟草公司遵义市公司 蠋蝽多巴胺D2受体AcDD2R基因及其激活剂与应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080168583A1 (en) * 1999-08-19 2008-07-10 Fincher Karen L Nucleic acid molecules and other molecules associated with plants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARAKANE Y ET AL.: "The Tribolium chitin synthase genes TcCHS] and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix''.", INSECT MOLECULAR BIOLOGY., vol. 15, no. 5, 2005, pages 453 - 463 *
BAUM JA ET AL.: "Control of coleopteran insect pest through RNA interference", NATURE BIOTECHNOLOGY, vol. 25, no. 11, 2007, pages 1322 - 1326 *
DATABASE GENBANK accession no. Q062153 *

Also Published As

Publication number Publication date
US20160000086A1 (en) 2016-01-07
US10182571B2 (en) 2019-01-22
WO2014100879A8 (pt) 2014-09-04
AR094337A1 (es) 2015-07-29
BR102012033539A2 (pt) 2015-08-18
BR102012033539A8 (pt) 2021-08-24
WO2014100879A2 (pt) 2014-07-03

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