WO2017014494A1 - Composition cosmétique pour rhytidectomie cutanée et prévenir le vieillissement, contenant un extrait de toile d'araignée - Google Patents

Composition cosmétique pour rhytidectomie cutanée et prévenir le vieillissement, contenant un extrait de toile d'araignée Download PDF

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Publication number
WO2017014494A1
WO2017014494A1 PCT/KR2016/007698 KR2016007698W WO2017014494A1 WO 2017014494 A1 WO2017014494 A1 WO 2017014494A1 KR 2016007698 W KR2016007698 W KR 2016007698W WO 2017014494 A1 WO2017014494 A1 WO 2017014494A1
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WIPO (PCT)
Prior art keywords
cobweb
cosmetic composition
spider web
peptide
spider
Prior art date
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Ceased
Application number
PCT/KR2016/007698
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English (en)
Korean (ko)
Inventor
이상정
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coseedbiopharm Co Ltd
Oberon Cosmetic Co Ltd
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Coseedbiopharm Co Ltd
Oberon Cosmetic Co Ltd
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Filing date
Publication date
Priority claimed from KR1020150144693A external-priority patent/KR101648494B1/ko
Application filed by Coseedbiopharm Co Ltd, Oberon Cosmetic Co Ltd filed Critical Coseedbiopharm Co Ltd
Publication of WO2017014494A1 publication Critical patent/WO2017014494A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a skin lifting webs and the anti-aging cosmetic composition containing the extract, specifically a red deokdari mushroom webs or webs peptide has a molecular weight of 10 ⁇ 70 KDa (Laetiporus sulphureus var. miniatus ) relates to a cosmetic composition exhibiting excellent antioxidant effect, skin wrinkle improvement effect, skin moisturizing effect, elastase inhibitory effect and skin elasticity increasing effect by containing as a active ingredient spider web hydrolyzate obtained by hydrolysis by miniatus .
  • the stratum corneum (SC) of the skin is present in the outermost layer of the skin and protects our body from substances such as harmful microorganisms, ultraviolet rays, pollutants, and heat, and regulates skin moisture evaporation and skin moisture retention. It is often known as a skin barrier function and is known to have the largest surface area in our body. SC layer is composed of dead keratinocytes layered, and intercellular lipid components such as ceramide, cholesterol, and fatty acid attach dead keratinocytes to maintain the form of skin barrier.
  • This intercellular lipid component has a function of preventing skin penetration of foreign substances and preventing the amount of water evaporated from the surface of the skin, that is, transepideramal water loss (TEWL).
  • TEWL transepideramal water loss
  • NMF natural moisturizing factor
  • keratinocytes in healthy humans, reducing the tension on the surface of the skin and receiving moisture repulsion from keratin, which directly affects moisturizing and inhibits drying of the skin.
  • dry skin tends to dry out the skin's surface and lose its elasticity, leading to exfoliation. When the skin elasticity falls, wrinkles occur and the skin loses its vitality. Therefore, maintaining the skin's surface moisture is a good way to maintain skin elasticity.
  • Collagen is a major component of the extracellular matrix (ECM), which consists of two ⁇ 1 (I) chains and one ⁇ 2 (I) chain, which are degraded by Type I collagen in the dermal layer of the skin as it ages.
  • ECM extracellular matrix
  • the protein that supports this collagen is elastin.
  • Elastin is very elastic and has a property of returning to its original form even when it is contracted or relaxed. These elastin fibers maintain skin elasticity.
  • collagen and elastin decomposing enzymes are produced from various stimulants that cause external aging and internal aging, and collagen and elastin are decomposed to create wrinkles and lose elasticity. It is a major cause of this.
  • spiders are arthropods that inhabit about 30,000 species around the world, and about 60 species are known to live in Korea.
  • the habitats are found in various places such as the ground, above the ground, inside and outside the buildings, but the shape of the cobweb and the web is known to be different according to the types of spiders.
  • liquid protein in the spider's body meets with acid to make a spider web, but even though the spider web looks like a strand, it is seen as a strand of spider web with many overlapping web strands overlapping and touching.
  • spider webs are classified into catching, safety, egg protecting, and when males give food to female spiders.
  • Cobwebs are known to have good elasticity and are about 5 times stronger than steel's tensile strength when they become thick and have hydrophilicity and lipophilic properties without causing allergies to humans.
  • GPGGX / GPGQQ, GGX, etc. are common amino acids and Gly 37.1%. Ala 21.1%, Arg 7.6%, Ser 4.5%, Glu 4.4%, Pro 4.3%, Leu 3.8%.
  • Glu and Gly are hydrophilic and determine the role of water. Gly, Glu, Tyr, Ser and Leu have been reported to affect contractile action and increase molecular motion.
  • the spider web is used only for spinning techniques such as bandages, artificial fibers, bulletproof vests, the technology to apply the spider web to the cosmetic composition is insignificant.
  • the present invention contains a spider web hydrolyzate obtained by hydrolyzing spider web peptide or spider web having a molecular weight of 10 ⁇ 70 KDa by fermentation of Laetiporus sulphureus var. Miniatus as an active ingredient, excellent antioxidant effect, skin wrinkle improvement
  • An object of the present invention is to provide a cosmetic composition exhibiting an effect, a skin moisturizing effect, an elastase inhibitory effect, and an increase in skin elasticity.
  • the present invention provides a cosmetic composition
  • a cosmetic composition comprising a spider web peptide having a molecular weight of 10 ⁇ 70 KDa as an active ingredient.
  • Spider web peptide having a molecular weight of 10 ⁇ 70 KDa of the present invention exhibits excellent antioxidant effect, MMP-1 production inhibitory effect, collagen synthesis enhancement effect, elastase inhibitory effect.
  • the cosmetic composition containing a spider web peptide having a molecular weight of 10 ⁇ 70 KDa as an active ingredient exhibits excellent skin moisturizing effect, skin elasticity enhancing effect and skin wrinkle improvement effect. According to the experimental example below it can be seen that the spider web peptide having a molecular weight of less than 10 KDa, more than 70 KDa does not exert such excellent skin improvement effect.
  • the cobweb peptide is preferably obtained by hydrolyzing the cobweb with an acid or protease. More preferably, the hydrolyzed spider web peptide is obtained by using a molecular weight cut off resin.
  • the present invention is a red spider web spider ( Laetiporus) sulphureus var. miniatus ) Provides a cosmetic composition containing a spider web hydrolyzate obtained by hydrolysis by fermentation as an active ingredient.
  • Spider web hydrolyzate of the present invention exhibits excellent antioxidant effect, MMP-1 production inhibitory effect, collagen synthesis enhancement effect, elastase inhibitory effect.
  • the cosmetic composition containing the spider web hydrolyzate as an active ingredient exhibits an excellent skin moisturizing effect, skin elasticity enhancing effect and skin wrinkle improvement effect. According to the following experimental example, it was confirmed that the cosmetic composition containing the spider web hydrolyzate as an active ingredient exhibits an excellent skin improvement effect than the general spider web extract.
  • the red toad mushroom ( Laetiporus sulphureus var.miniatus ) belonging to the fungus of the basidiomycetes is native to Mt. Baekdu, Wolchulsan, Jiri, and Valwangsan, and is distributed throughout the tropical regions of Japan and Asia. This is a 1 year-old heartwood brownish fungi that regenerate on the conifers, trees, and stumps from spring to summer. Reddish-legged mushrooms have long been reported to be effective in regulating function, improving health, and anti-disease, but the exact pharmacological effects are unknown. In addition, it has not been disclosed about the excellent skin moisturizing effect, skin elasticity enhancing effect, skin wrinkle improvement effect of the spider web hydrolyzate obtained by hydrolyzing the spider web by red mandarin mushroom fermentation.
  • the cosmetic composition may be preferably for improving skin wrinkles, for improving skin elasticity, for moisturizing the skin.
  • the cobweb peptide or cobweb hydrolyzate preferably 0.00001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably 0.01 to 10% by weight relative to the total weight of the cosmetic composition.
  • the content of the spider web peptide or spider web hydrolyzate is less than 0.00001% by weight does not appear to improve the skin, when the content exceeds 30.0% by weight does not increase the apparent effect of increasing the content.
  • the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to spider web peptide or spider web hydrolyzate having a molecular weight of 10 ⁇ 70 KDa as an active ingredient, such as antioxidants, stabilizers, Conventional adjuvants such as solubilizers, vitamins, pigments and flavorings, and carriers.
  • Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.
  • the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide
  • cellulose derivatives polyethylene glycols
  • silicones bentonites
  • silicas talc or zinc oxide
  • a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.
  • liquid carrier diluents such as water, ethanol or propylene glycol
  • suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components.
  • Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
  • the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether.
  • Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin.
  • the soap is liquid soap, powdered soap, solid soap and oil soap
  • the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack
  • the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.
  • the cobweb hydrolyzate obtained by hydrolyzing a cobweb peptide or cobweb having a molecular weight of 10 to 70 KDa of the present invention by red mandarin mushroom fermentation has an antioxidant effect, an MMP-1 production inhibitory effect, collagen synthesis
  • the cosmetic composition containing the cobweb peptide or the cobweb hydrolyzate as an active ingredient exhibits an enhancement effect and an elastase inhibitory effect, and has an excellent skin moisturizing effect, skin elasticity enhancing effect and skin wrinkle improvement effect.
  • the webs 10.0 g collected from wild and interior 100 g was dispersed in water, a red deokdari mushroom (Laetiporus sulphureus) CS0218 (KFCC 11494P ) strain culture was added. To this, 3% glucose was added as a carbon source, and 0.3% of peptone was further added and cultured. Cultivation was carried out using a 5 L fermenter for 7 days, maintained at 37 °C, pH 6. After the culture, the culture medium was centrifuged to remove the culture medium first.
  • the cobweb fermented product from which culture was first removed was refluxed three times for 5 hours by adding ethanol to a final 70% (V / V) ethanol aqueous solution, and then cooled, and filtered through whatman # 10 filter paper. It was. The filtrate thus obtained was subjected to final filtration using a filter of 0.25 ⁇ M to obtain spider web hydrolysate.
  • the spider web hydrolysate was dissolved in 50% butylene glycol aqueous solution to 1% and used in the following experimental example.
  • the spider web hydrolysate obtained by hydrolyzing the spider web by red mandarin mushroom fermentation was referred to as the 'red mandarin mushroom- spider web hydrolysate'.
  • the spider web peptide hydrolyzed by the acid in Preparation Example 1 was fractionated into a column tube filled with Sephadex resin (Sephadex LH-20) to obtain a spider web peptide of less than 10 KDa. Thereafter, the low molecular cobweb peptide was dissolved in 50% butylene glycol aqueous solution to 1% and used in the following experimental example.
  • the spider web peptide hydrolyzed by the acid in Preparation Example 1 was fractionated into a column tube filled with Sephadex resin (Sephadex LH-20) to obtain a spider web peptide of more than 70 KDa. Thereafter, the polymer spider web peptide was dissolved in 50% butylene glycol aqueous solution to 1% and used in the following experimental example.
  • the spider web peptide hydrolyzed by the protease in Preparation Example 2 was fractionated into a column tube filled with Sephadex resin (Sephadex LH-20) to obtain a spider web peptide of less than 10 KDa. Thereafter, the low molecular cobweb peptide was dissolved in 50% butylene glycol aqueous solution to 1% and used in the following experimental example.
  • the cobweb peptide hydrolyzed by the protease in Preparation Example 2 was fractionated into a column tube filled with Sephadex resin (Sephadex LH-20) to obtain more than 70 KDa cobweb peptide. Thereafter, the polymer spider web peptide was dissolved in 50% butylene glycol aqueous solution to 1% and used in the following experimental example.
  • the free radical scavenging rate was measured by using DPPH method to determine the skin antioxidant effect of the spider web peptide and red thigh mushroom-spider hydrolyzate of 10 ⁇ 70 KDa prepared in Preparation Examples 1 and 3.
  • the DPPH method is a method of measuring the antioxidant effect by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical.
  • the free radical scavenging rate (%) can be measured at a wavelength of 560 nm by comparing the degree of decrease in absorbance of DPPH by the sample to the absorbance of the blank.
  • a reagent used 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem.
  • the blank test is performed in the same manner as in the case of measuring the absorbance St, except that distilled water is used instead of the sample solution to measure the absorbance Bt.
  • Blank of the blank test was measured in the same manner as when the absorbance St was measured except that methanol was used instead of the 0.1 mM DPPH solution and distilled water was used instead of the sample solution.
  • Fibroblasts (Korea Cell Line Bank, Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 ⁇ 10 6 cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. Incubated for 24 hours at. Subsequently, after adding TGF- ⁇ to the spider web peptides, red spider mushroom-spider hydrolyzate, spider web extract, and the positive control group of Preparation Examples 1 to 3 and Comparative Preparation Examples 1-1 to 3, and further cultured for 48 hours It was. At this time, the medium without the sample was used as a control.
  • Fibroblasts human normal epithelial cells, were seeded in 48-well microplates at 1 ⁇ 10 6 cells per well and incubated for 24 hours in DMEM medium. Then, TGF- ⁇ was added to the spider web peptides, red spider mushroom-spider hydrolyzate, spider web extract, and the positive control group of Preparation Examples 1 to 3 and Comparative Preparation Examples 1 to 3, and then further cultured for 48 hours. . At this time, the medium without the sample was used as a control.
  • the spider web peptide, spider web hydrolyzate and spider web extract obtained in Preparation Examples 1 to 3 and Comparative Preparation Examples 1-1 to 3 were prepared at the concentrations shown in Table 4 below.
  • 100 ⁇ l of each sample was put in a 96-well plate and 60 ⁇ l of Tris buffer (Sigma T1503).
  • 20 ⁇ l of substrate N-Suc-Ala-Ala-Ala-NA-8.8mM
  • 20 ⁇ l of enzyme (Elastase, Sigma E0258, 10 ⁇ g / ml) was added thereto. After reacting for a minute, absorbance was measured at 410 nm. At this time, the control group had a buffer solution instead of the sample.
  • Elastase inhibition rate (%) was calculated according to Equation 4 below, the results are shown in Table 4.
  • the components are mixed in the order of ABC as shown in Table 5 below to prepare a cosmetic composition containing spider web peptide, red spider-spider hydrolyzate or spider web extract, and then Formulation Examples 1 to 3, Comparative Formulation Examples, respectively. Referred to 1-5.
  • the cosmetic compositions of Formulation Examples 1 to 3 were applied to the D, E, F, G, and H groups on the face for 8 weeks, twice a day for the cosmetic compositions of Comparative Formulations 1 to 5, respectively.
  • Moisturizing effect was evaluated using a moisture meter (Corneometer CM 820, Corage + Khazaka, Germany) before use, 2 to 4 weeks after use. The experimental results are shown in Table 6 below.
  • the cosmetics (10-70 KDa cobweb peptide or red thigh mushroom-spider hydrolyzate containing cosmetic composition) of Formulation Examples 1-3 contained Comparative Formulation Examples 1-5 ( ⁇ 10 KDa, more than 70 KDa cobweb peptide or cobweb extract) Cosmetic composition) was confirmed to exhibit an excellent skin moisturizing effect.
  • the cosmetic composition containing a spider web hydrolyzate obtained by hydrolyzing spider web peptide having a molecular weight of 10 ⁇ 70 KDa and spider web by red mandarin mushroom fermentation exhibits excellent skin moisturizing effect.
  • Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 5 were used twice a day in the morning and evening for 20 women in their 30s and 50s. Subsequently, skin elasticity of the area around the eyes was measured using a Cutometer SEM 575 before and after 2 to 4 weeks of use. After washing the instrument with lukewarm water, it was measured after 20 minutes in constant temperature and humidity room. The results were as shown in Table 7 below.
  • Formulation Examples 1 to 3 (10 ⁇ 70 KDa spider web peptide or red spider mushroom-spider hydrolyzate containing cosmetic composition) Comparative Formulation Examples 1 to 5 (less than 10 KDa, more than 70 KDa spider web peptide or cobweb extract containing cosmetics It was confirmed that the exertion effect of skin elasticity than the composition).
  • the cosmetic composition containing a spider web hydrolyzate obtained by hydrolyzing spider web peptide having a molecular weight of 10 ⁇ 70 KDa and spider web by red mandarin mushroom fermentation exhibits excellent skin elasticity promoting effect. .
  • the skin wrinkle improvement effect was evaluated by visual observation of an experienced doctor before and after using the product for three months, and the results of wrinkle improvement based on this evaluation are shown in Table 8 below.
  • Formulation Examples 1 to 3 (cosmetic composition containing 10 ⁇ 70 KDa spider web peptide or red spider mushroom- spider web hydrolyzate) is less than Comparative Formulation Examples 1 and 2 (cosmetic composition containing spider web peptide less than 10 KDa or greater than 70 KDa) It can be seen that the skin has two times higher wrinkle improvement.

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Abstract

La présente invention concerne une composition cosmétique contenant, comme principe actif, un peptide de toile d'araignée ayant un poids moléculaire de 10 à 70 kDa ou un hydrolysat de toile d'araignée obtenu par hydrolyse d'une toile d'araignée par l'intermédiaire de la fermentation de Laetiporus sulphureus var. miniatus. Le peptide de toile d'araignée ayant un poids moléculaire de 10 à 70 kDa ou l'hydrolysat de toile d'araignée obtenu par hydrolyse de toile d'araignée par l'intermédiaire de la fermentation de Laetiporus sulphureus var. miniatus présente un excellent effet antioxydant, effet inhibiteur de production de MMP-1, effet favorisant la synthèse de collagène, et effet inhibiteur d'élastase, et la composition cosmétique de la présente invention contenant, comme principe actif, le peptide de toile d'araignée ou l'hydrolysat de toile d'araignée présente d'excellents effets d'hydratation de peau, d'élasticité de peau et d'atténuation de rides cutanées.
PCT/KR2016/007698 2015-07-17 2016-07-14 Composition cosmétique pour rhytidectomie cutanée et prévenir le vieillissement, contenant un extrait de toile d'araignée Ceased WO2017014494A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020150101686A KR101565542B1 (ko) 2015-07-17 2015-07-17 거미줄 추출물을 함유하는 피부 리프팅 및 노화 방지용 화장료 조성물
KR10-2015-0101686 2015-07-17
KR10-2015-0144693 2015-07-17
KR1020150144693A KR101648494B1 (ko) 2015-10-16 2015-10-16 거미줄 가수분해물을 함유하는 피부 리프팅 및 노화 방지용 화장료 조성물

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EP3494980A1 (fr) * 2017-12-08 2019-06-12 Oguzhan Doganlar Extrait de toile d'araignée, sa methode de préparation, et son utilisation dans le traitement du cancer de la prostate
CN117357427A (zh) * 2023-12-07 2024-01-09 深圳市灵蛛科技有限公司 一种重组蛛丝蛋白的用途

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KR101652953B1 (ko) 2016-01-15 2016-08-31 (주)넥스젠바이오텍 열 안정성이 증가한 인간성장호르몬 융합단백질 및 이를 유효성분으로 함유하는 피부 주름 개선 및 탄력 유지용 화장료 조성물
KR102101952B1 (ko) * 2018-11-23 2020-04-27 주식회사 코씨드바이오팜 거미줄 추출물을 함유하는 피부 미백용 및 자외선에 의한 피부 손상 예방용 화장료 조성물
KR102346006B1 (ko) 2021-05-21 2022-01-03 (주)파이온텍 거미줄 유래 펜타펩타이드 및 이를 함유하는 항노화용 조성물

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11269061A (ja) * 1998-02-11 1999-10-05 L'oreal Sa 少なくとも一つの天然のまたは組換えクモの糸タンパク質または類似体を含む化粧用または皮膚科用組成物
CN103126918A (zh) * 2013-02-18 2013-06-05 崇州市地龙海龙生物制品开发研究所 一种抗皱霜制备方法
KR20140119094A (ko) * 2012-01-04 2014-10-08 모멘티브 퍼포먼스 머티리얼즈 인크. 이온성 실리콘 및 막형성제를 함유하는 퍼스널 케어 조성물
KR20150007368A (ko) * 2013-07-09 2015-01-21 한불화장품주식회사 미숙 토마토 발효물을 함유하는 항노화 기능성 화장료 조성물
KR101508614B1 (ko) * 2014-08-25 2015-04-08 한솔바이오 주식회사 스파이더 실크 단백질, 거머리 추출물, 봉독 추출물 및 뱀독 유사 펩타이드 복합물로 제조된 포이즌 컴플렉스를 유효성분으로 함유하는 항노화 화장료 조성물

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11269061A (ja) * 1998-02-11 1999-10-05 L'oreal Sa 少なくとも一つの天然のまたは組換えクモの糸タンパク質または類似体を含む化粧用または皮膚科用組成物
KR20140119094A (ko) * 2012-01-04 2014-10-08 모멘티브 퍼포먼스 머티리얼즈 인크. 이온성 실리콘 및 막형성제를 함유하는 퍼스널 케어 조성물
CN103126918A (zh) * 2013-02-18 2013-06-05 崇州市地龙海龙生物制品开发研究所 一种抗皱霜制备方法
KR20150007368A (ko) * 2013-07-09 2015-01-21 한불화장품주식회사 미숙 토마토 발효물을 함유하는 항노화 기능성 화장료 조성물
KR101508614B1 (ko) * 2014-08-25 2015-04-08 한솔바이오 주식회사 스파이더 실크 단백질, 거머리 추출물, 봉독 추출물 및 뱀독 유사 펩타이드 복합물로 제조된 포이즌 컴플렉스를 유효성분으로 함유하는 항노화 화장료 조성물

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* Cited by examiner, † Cited by third party
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EP3494980A1 (fr) * 2017-12-08 2019-06-12 Oguzhan Doganlar Extrait de toile d'araignée, sa methode de préparation, et son utilisation dans le traitement du cancer de la prostate
CN117357427A (zh) * 2023-12-07 2024-01-09 深圳市灵蛛科技有限公司 一种重组蛛丝蛋白的用途

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