WO2018043459A1 - Récipient du type sac pour cellules - Google Patents

Récipient du type sac pour cellules Download PDF

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Publication number
WO2018043459A1
WO2018043459A1 PCT/JP2017/030862 JP2017030862W WO2018043459A1 WO 2018043459 A1 WO2018043459 A1 WO 2018043459A1 JP 2017030862 W JP2017030862 W JP 2017030862W WO 2018043459 A1 WO2018043459 A1 WO 2018043459A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
gas
bag
cell
gas sealing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2017/030862
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English (en)
Japanese (ja)
Inventor
貴彦 戸谷
郷史 田中
亮 末永
直己 高橋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Seikan Group Holdings Ltd
Original Assignee
Toyo Seikan Group Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Seikan Group Holdings Ltd filed Critical Toyo Seikan Group Holdings Ltd
Publication of WO2018043459A1 publication Critical patent/WO2018043459A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present invention relates to a cell-like container for cell culture or storage.
  • Patent Document 1 a film material (soft packaging material) for forming a container bottom surface is formed in a concavo-convex shape.
  • a culture bag provided with a recess is proposed. According to this, compared with the culture bag which has a planar bottom face, it is supposed that the proliferation rate of a cell can be raised (refer paragraph [0081] of patent document 1).
  • a bag-like container made of a soft wrapping material like the culture bag proposed in Patent Document 1, is suitable for closed culture with a low risk of contamination, and is therefore widely used for cell culture applications.
  • a container is easily deformed due to the nature of the material. Therefore, in patent document 1, it is made possible to hold
  • Patent Document 1 air holes are provided in the holding tray so that oxygen permeation into the culture bag is not hindered by the holding tray (see paragraph [0072] of Patent Document 1). In addition, there is a limit, and it is inevitable that oxygen permeation is hindered at the portion in contact with the holding tray.
  • the present inventors have provided a container provided in the container without separately preparing a member for holding the shape of the bag-shaped container made of the soft wrapping material for cell culture or storage. As a result of intensive studies so that the shape can be maintained, the present invention has been completed.
  • the cell-like bag-like container according to the present invention is a bag-like container made of a soft wrapping material used for cell culture or storage, and includes a storage part and a gas enclosure partitioned from the storage part.
  • the housing portion is supported by the gas-filled portion in which the bottom portion of the housing portion is separated from the placement surface on which the container is placed by the bag-filled portion and the gas-filled portion inflated by being filled with gas.
  • the configuration is made possible.
  • the present invention it is possible to maintain the shape of the housing portion, and it is also possible to absorb an impact from the outside by the gas sealing portion in which the gas is sealed.
  • FIG. 1 the outline of the bag-shaped container for cells which concerns on 1st embodiment of this invention is shown.
  • a container 1 shown in FIG. 1 is an example in which the present invention is applied to a culture bag made of a soft wrapping material suitable for closed culture, and the soft wrapping material forming the container 1 can perform cell culture in a closed system. It is preferable that it has oxygen permeability and carbon dioxide permeability necessary for cell culture so that it can do. Furthermore, in order to realize high cell growth efficiency, it is preferable that it has low cytotoxicity, low elution property, and radiation sterilization suitability, so that the progress of cell culture and the state of cells can be observed. It is preferable to have transparency to the extent that it can be seen through.
  • soft packaging materials include thermoplastics such as polyethylene, polypropylene, ethylene-vinyl acetate copolymer, polyester, silicone elastomer, polystyrene elastomer, tetrafluoroethylene-hexafluoropropylene copolymer (FEP), and the like.
  • thermoplastics such as polyethylene, polypropylene, ethylene-vinyl acetate copolymer, polyester, silicone elastomer, polystyrene elastomer, tetrafluoroethylene-hexafluoropropylene copolymer (FEP), and the like.
  • FEP tetrafluoroethylene-hexafluoropropylene copolymer
  • a resin film made of a resin can be used. These resins may be used in a single layer or may be used by laminating the same or different resins.
  • the container 1 is bag-made using such a soft wrapping material, is provided with a housing portion 2, and a gas sealing portion 3 partitioned from the housing portion 2 is integrally bag-made.
  • a gas sealing portion 3 partitioned from the housing portion 2 is integrally bag-made.
  • FIG. 1 a square-shaped storage part 2 whose periphery is heat-sealed, and the storage part 2 is partitioned by heat sealing It is possible to form a bag integrally with the gas sealing portion 3 formed.
  • part in which the soft-packing materials were heat-sealed is shown with a halftone dot.
  • the storage unit 2 is a place where cells are stored together with a culture solution and the like, and the cells are stored before and after culture.
  • the housing portion 2 can be formed into a predetermined shape by molding means such as vacuum forming, pressure forming, press forming or the like simultaneously with heat sealing or prior to heat sealing.
  • the housing portion 2 is formed on the bottom surface of the housing portion 2.
  • a plurality of recesses 20 serving as cell culture parts are provided.
  • the concave portion 20 provided on the bottom surface of the accommodating portion 2 suppresses the movement of the cells in the accommodating portion 2, allows the cells being cultured to remain in the single concave portion 20, and collects the cells at the bottom of the concave portion 20 for efficient culture.
  • it can be formed in a spherical crown shape having an opening diameter (diameter) of 1.5 to 10 mm and a depth of 0.1 mm or more.
  • the container 2 is provided with an inlet / outlet port 21 to which a liquid feeding tube can be connected.
  • a liquid feeding tube can be connected.
  • the medium is supplied from the medium bag in which the medium is stored, or the cultured cells are collected into the collection bag after the culture is completed. This operation can be performed while maintaining the closed system via the liquid feeding tube connected to the injection port 21.
  • the injection port 21 is formed of a tubular member through which a culture medium, cells, and the like can circulate.
  • a culture medium for example, injection molding, extrusion molding, or the like using a thermoplastic resin such as polyethylene, polypropylene, vinyl chloride, polystyrene elastomer, or FEP.
  • a thermoplastic resin such as polyethylene, polypropylene, vinyl chloride, polystyrene elastomer, or FEP.
  • FEP polystyrene elastomer
  • the storage portion is formed from the base end thereof. 2 can be provided with a port blocking prevention piece projecting into 2.
  • a gas such as nitrogen gas or air having low permeability with respect to the soft packaging material (resin film) used for the container 1 is sealed in the gas sealing portion 3, and the gas sealing portion 3 expands by sealing the gas. It has become.
  • the container 1 is made using the soft packaging material as described above.
  • a gas barrier layer may be provided as necessary at a site where the gas sealing portion 3 of the soft packaging material is formed.
  • the gas enclosure portion 3 may be filled with gas when the container 1 is made, but if the gas enclosure portion 3 is in an inflated state, it will be bulky during storage and during storage. There is also a concern that the gas sealed in the gas escapes and the gas sealing part 3 is deflated. For this reason, it is preferable that the gas sealing part 3 is provided with a gas sealing port 31 so that the gas can be sealed in the gas sealing part 3 when the container 1 is used.
  • the gas sealing port 31 is not particularly limited as long as the gas sealing port 31 can be opened and closed when the gas is sealed in the gas sealing part 3, but the check is made to facilitate the gas sealing operation. It preferably has a valve structure.
  • the gas sealing portion 3 is formed on the peripheral side of the housing portion 2 so as to surround the housing portion 2 except for a portion where the injection port 21 is provided.
  • the expanded gas enclosure part 3 functions as a support
  • 2A shows the AA end face of FIG. 1
  • FIG. 2B shows a state in which the container 1 inflated by enclosing the gas in the gas enclosure 3 is placed on the placement surface S. Is shown.
  • the shape of the accommodating portion 2 particularly the shape of the recess 20 provided on the bottom surface of the accommodating portion 2, without separately preparing a member for holding the shape.
  • the intended purpose of providing the concave portion 20 on the bottom surface of the accommodating portion 2 is not hindered, and the cells can be collected and cultured efficiently at the bottom portion of the concave portion 20.
  • the bottom surface of the housing portion 2 can be brought into non-contact with the mounting surface S, there is no possibility of impeding permeation of oxygen and carbon dioxide necessary for cell culture.
  • the gas enclosure 3 is disposed so as to be formed on the peripheral edge side of the accommodating portion 2, and the container 1 is placed by the gas enclosure 3 that is expanded by being encapsulated with the gas.
  • the housing part 2 can be supported in a state where the bottom surface side of the housing part 2 is separated from the placement surface S
  • the arrangement of the gas sealing part 3 is not limited to the example shown in FIG.
  • FIG. 3 shows another example in which the gas enclosing part 3 is arranged on the peripheral side of the accommodating part 2.
  • one gas enclosing part 3 is arranged along the three sides of the accommodating part 2.
  • two gas sealing portions 3 that are bent at both ends and formed in a U shape are opposed to each other with the accommodating portion 2 therebetween.
  • two gas sealing portions 3 that are bent at one end side and formed in an L shape are arranged so as to face each other with the accommodating portion 2 therebetween. May be.
  • the size of the container 1 is large, it can be arranged as shown in FIG.
  • the end of one gas enclosure 3 and the end of the other gas enclosure 3 arranged so as to oppose each other via the housing 2. If the part is separated, the accommodating part 2 may hang down.
  • the reinforcing member 30 made of a rigid body can be disposed between both end portions to prevent the housing portion 2 from drooping.
  • the container 1 can be placed on the mounting table by locking the holes 32 drilled at the four corners thereof with fixing pins standing on the mounting table.
  • the gas sealing portion 3 along at least two opposing sides of the housing portion 2, the bottom surface side of the housing portion 2 is placed with respect to the placement surface S.
  • the accommodating part 2 can be supported in the separated state.
  • the usage mode of the container 1 is not limited to this.
  • the gas sealing portion 3 that is expanded by being filled with gas can also be expected to have a function as a buffer material that absorbs an impact when the container 1 drops. From this, the container 1 is used as a storage container excellent in impact resistance used for the preservation of cells, utilizing the fact that the gas sealing part 3 in which the gas is sealed can absorb the impact from the outside. Is also possible.
  • the container when cryopreserving cells, the container is generally frozen with liquid nitrogen at -196 ° C, but the impact resistance of the container is extremely reduced due to freezing, and it is accidentally dropped when the container is removed. Then, the container may be damaged. If the container 1 of the present embodiment is used as a cryopreservation container and gas is enclosed in the gas enclosure 3 and frozen, when the container 1 is taken out, the gas enclosure 3 swells and functions as a buffer material, and the container 2 Can be protected. After thawing, cell culture can be performed as it is.
  • the gas sealing part 3 is formed on the peripheral edge side of the housing part 2, but in this embodiment, as shown in FIG.
  • the gas enclosure 3a is formed.
  • the container 1 shown in FIG. 4 includes, for example, a soft packaging material that forms the top surface side of the housing portion 2, a soft packaging material that forms the bottom surface side of the housing portion 2, and a soft packaging material that forms the gas sealing portion 3a.
  • the injection port 21 and the gas sealing port 31 are sandwiched at predetermined positions, and the periphery of these soft wrapping materials is heat-sealed, so that it is partitioned from the accommodating portion 2 and overlapped below it.
  • the formed gas sealing part 3a can be made into a bag integrally with the accommodating part 2.
  • the housing portion 2 When gas is sealed in the gas sealing portion 3a formed to overlap the lower portion of the housing portion 2, the housing portion 2 is lifted by the expanded gas sealing portion 3a, and the placement surface S on which the container 1 is placed is placed.
  • the accommodating part 2 can be supported in a state where the bottom surface side of the accommodating part 2 is separated. Thereby, it becomes possible to hold
  • the gas enclosure part 3b can be formed also on the upper part of the accommodating part 2, and can be formed.
  • the container 1 shown in FIG. 5 includes, for example, a soft packaging material that forms the gas sealing portion 3b, a soft packaging material that forms the top surface side of the housing portion 2, and a soft packaging material that forms the bottom surface side of the housing portion 2.
  • the peripheral edges of these soft wrapping materials are heat-sealed, thereby The gas sealing portions 3 a and 3 b that are partitioned from each other and overlapped with the upper and lower portions thereof can be formed integrally with the housing portion 2.
  • the container 1 When the gas sealing part 3a is overlapped and formed only on one side below the housing part 2, when the gas sealing part 3a is filled with gas and inflated, the container 1 may be warped in a bow. The warping of the container 1 can be suppressed by forming the gas sealing portions 3a and 3b so as to overlap the upper and lower sides of the housing portion 2.
  • the present embodiment is different from the first embodiment described above in that the gas sealing portions 3a and 3b are formed below the storage portion 2 or stacked one above the other. Since the configuration other than this is common to the above-described first embodiment, a duplicate description is omitted.
  • this embodiment can also be used as a storage container with excellent impact resistance used for storage of cells.
  • the gas enclosures 3a and 3b are formed so as to overlap the upper and lower sides of the housing part 2, the entire surface of the housing part 2 is covered by the gas enclosures 3a and 3b that can absorb external impacts on the surface. Higher shock absorbing performance can be exhibited.
  • the cell-like bag-like container according to the present invention includes the accommodating portion 2 and the gas enclosing portion 3 partitioned from the accommodating portion 2 is integrally formed, and the gas enclosing portion 3 inflated by enclosing the gas. If the container 2 can be supported in a state where the bottom surface side of the container 2 is separated from the placement surface S on which the container 1 is placed, the other detailed configuration is the same as that of the above-described embodiment. It can change suitably, without being limited to. Further, the detailed configurations described in the above-described embodiments can be appropriately selected and combined.
  • the present invention can be widely used as a bag-like container for cells used for cell culture or storage in the fields of pharmaceutics and biochemistry.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un récipient (1) pourvu d'une unité de logement (2); une unité d'enceinte (3) de gaz, qui est séparée de l'unité de logement (2), est intégrée à l'unité de logement (2) pour former un sac, de sorte que l'unité de logement (2) peut être supportée par l'unité d'enceinte (3) de gaz, laquelle est dilatée sous l'action d'un gaz contenu dans celle-ci, dans un état tel que le côté inférieur de l'unité de logement (2) est espacé d'une surface de montage (S) sur laquelle le récipient (1) doit être monté. Selon cette configuration, dans le cas où un récipient du type sac, constitué d'un matériau d'emballage souple, est utilisé pour la culture ou le stockage de cellules, il est possible de maintenir la forme de l'unité de logement (2) prévue dans le récipient sans qu'il soit nécessaire de fournir séparément un élément pour maintenir ladite forme.
PCT/JP2017/030862 2016-09-02 2017-08-29 Récipient du type sac pour cellules Ceased WO2018043459A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016-171764 2016-09-02
JP2016171764A JP2018033423A (ja) 2016-09-02 2016-09-02 細胞用バッグ状容器

Publications (1)

Publication Number Publication Date
WO2018043459A1 true WO2018043459A1 (fr) 2018-03-08

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Application Number Title Priority Date Filing Date
PCT/JP2017/030862 Ceased WO2018043459A1 (fr) 2016-09-02 2017-08-29 Récipient du type sac pour cellules

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JP (1) JP2018033423A (fr)
TW (1) TW201811176A (fr)
WO (1) WO2018043459A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020039865A1 (fr) * 2018-08-24 2020-02-27 富士フイルム株式会社 Appareil de culture de cellules et procédé de transfert de suspension de cellules
WO2021033280A1 (fr) * 2019-08-21 2021-02-25 株式会社サンプラテック Kit pour le transport atmosphère-gaz-cellule

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7264360B1 (ja) 2022-06-29 2023-04-25 ティシューバイネット株式会社 立体細胞構造体作製する培養バッグ
CN116439232B (zh) * 2023-06-14 2023-09-29 山东第一医科大学附属省立医院(山东省立医院) 一种胸外科病变组织收取保藏装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02255077A (ja) * 1989-03-28 1990-10-15 Kawasumi Lab Inc 培養バッグ
JPH037575A (ja) * 1989-03-30 1991-01-14 Shimadzu Corp 細胞培養装置
JP2005323588A (ja) * 2004-04-13 2005-11-24 Toyo Seikan Kaisha Ltd 培養二重容器および培養方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02255077A (ja) * 1989-03-28 1990-10-15 Kawasumi Lab Inc 培養バッグ
JPH037575A (ja) * 1989-03-30 1991-01-14 Shimadzu Corp 細胞培養装置
JP2005323588A (ja) * 2004-04-13 2005-11-24 Toyo Seikan Kaisha Ltd 培養二重容器および培養方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020039865A1 (fr) * 2018-08-24 2020-02-27 富士フイルム株式会社 Appareil de culture de cellules et procédé de transfert de suspension de cellules
WO2021033280A1 (fr) * 2019-08-21 2021-02-25 株式会社サンプラテック Kit pour le transport atmosphère-gaz-cellule

Also Published As

Publication number Publication date
JP2018033423A (ja) 2018-03-08
TW201811176A (zh) 2018-04-01

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