WO2018069578A1 - Unité de culture et kit pour cultiver des cellules - Google Patents

Unité de culture et kit pour cultiver des cellules Download PDF

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Publication number
WO2018069578A1
WO2018069578A1 PCT/FI2017/050717 FI2017050717W WO2018069578A1 WO 2018069578 A1 WO2018069578 A1 WO 2018069578A1 FI 2017050717 W FI2017050717 W FI 2017050717W WO 2018069578 A1 WO2018069578 A1 WO 2018069578A1
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WO
WIPO (PCT)
Prior art keywords
cultivation
chamber
unit
cultivation unit
passageway
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI2017/050717
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English (en)
Inventor
Jouni AHTINEN
Lauri REUTER
Heiko Rischer
Juha-Pekka Pitkänen
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VTT Technical Research Centre of Finland Ltd
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VTT Technical Research Centre of Finland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Publication of WO2018069578A1 publication Critical patent/WO2018069578A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions

Definitions

  • the present disclosure relates to cultivation of plant cells, particularly for the production of food. More specifically, the disclosure relates to a cultivation unit and set for cultivating cells, particularly plant cells.
  • a novel cultivation unit for cultivating cells is therefore herein proposed.
  • the unit includes a sterile boundary isolating the cultivation unit from external contaminants, a cultivation chamber, a water inlet configured to feed water into the cultivation chamber, and an air inlet configured to feed air into the cultivation chamber.
  • a first auxiliary chamber is connected to the cultivation chamber via a passageway, which may be manipulated from a closed state to an open state so as to introduce a substance contained in the cultivation chamber and a substance contained in the first auxiliary chamber to each other.
  • FIGURE 1 illustrates a schematic layout of a cultivation unit according to one embodiment
  • FIGURE 2A illustrates a schematic lateral cross-sectional view of the cultivation unit of
  • FIGURE 1 showing the structure of a filter between two superposed film layers
  • FIGURE 2B illustrates a schematic cross-sectional view of the cultivation unit of FIGURE
  • FIGURE 3A illustrates a side elevation view an actuated passageway between the first and second chamber
  • FIGURE 3B illustrates an explosion view of the device of FIGURE 3 A
  • FIGURE 3C illustrates a perspective view of the device of FIGURE 3 A
  • FIGURE 4 illustrates a schematic layout of a cultivation unit according to another embodiment
  • FIGURE 5 illustrates a schematic layout of a variant of the cultivation unit according to
  • FIGURE 6 illustrates a schematic layout of a variant of the cultivation unit according
  • the cultivation unit 100 takes the form of a single-use unit intended to be used as a stand-alone device.
  • the cultivation unit 100 is preferably constructed as a disposable bag that is self-contained in that it can be attached to air and water supplies at a household or other non- laboratory setting.
  • the cultivation unit 100 is delimited by a sterile boundary enabling the unit to be attached, for example, to a household water tap.
  • all the chambers of the cultivation unit 100 are within the sterile boundary.
  • all the components of the cultivation unit that cater for the production of cells are delimited by the sterile boundary.
  • the sterile boundary is provided by sealing the outer perimeter of the unit.
  • the inlets to the inside of the sealed perimeter have been provided with sterile filters.
  • the filters are integrated into the cultivation unit so as to form part of the sterile boundary.
  • sterile filter refers to a filter, which has a pore size of at most 0.22 ⁇ .
  • the cultivation unit 100 contains an air inlet 60 for feeding air into the bag.
  • the air inlet 60 has been provided with an inlet air filter 61, which is a sterile filter.
  • the inlet air filter 61 may include a port for forced aeration (not shown), such as a tube connection to an air pump.
  • the cultivation unit 100 also includes a water inlet 50 with a preferred two-stage filtering configuration.
  • the water inlet 50 includes a carbon filter 51, which receives water from outside the unit 100 and passes the carbon filtered water onto a sterile filter 52 arranged in series with the carbon filter 51. Accordingly, the perimeter and inlets of the cultivation unit 100 form a sterile boundary.
  • the cultivation unit 100 may be constructed as a bag.
  • the material for the bag could be selected from a host of different materials non-permeable to water.
  • the material is a food-grade polymer, which is pliable and transparent so that resulting bag will be supple and see-through.
  • materials include, for example, polypropylene and polyethylene.
  • pliable refers to a material or structure that is flexible enough to endure bending during transport and normal use. The unit is therefore supple enough to withstand handling and will experience flexible deformation when subjected to a load equal to its own weight.
  • One particularly suitable material is low-density polyethylene film.
  • the material may have the properties as set forth in Table 1. Table 1: Material properties for the cultivation unit.
  • the perimeter of the bag may be formed by superposing two layers of the sheet raw material and sealing the edges together by plastic welding, for example. Openings are left for the water and air supplies 50, 60.
  • the filters 51, 52, 61 may be integrated into the cultivation unit 100 by enclosing the filters 51, 52, 61 by the superposed sheet layers of the cultivation unit 100.
  • FIGURES 2A and 2B One such construction is presented in FIGURES 2A and 2B. As can be seen best from FIGURE 2 A, there are two superposed layers 101, 102 attached to each other along a seam 103.
  • the first film layer 101 is open at one end (left in FIGURE 2 A) and a closed at the opposite end (right in FIGURE 2A).
  • the second film layer 102 is also open at one end (right in FIGURE 2A) and closed at the opposite end (left in FIGURE 2 A), but reversed in respect to the first film layer 101.
  • the filter 81 is arranged between the film layers 101, 102 and attached there to with the seam 103, which may be a plastic weld seam, adhesive seam, etc.
  • the filter 81 therefore divides the inner volume of the bag in two.
  • the filter may be any of the filters herein described.
  • the entry flow indicated with a thickened dashed arrow line I entering the bag at one end cannot exit through the opposite end. Instead, it is forced to go through the filter. Conversely, the filtered flow must exit from the open end of the second film layer as an exit flow indicated thickened dashed arrow line O.
  • FIGURE 2B shows the same structure from a different angle so as to demonstrate the bag-like shape of the unit.
  • the cultivation unit 100 contains several sub- volumes, which may be brought into fluid communication with one another.
  • the cultivation unit 100 includes a cultivation chamber 10 with dimensions set so as to be able to accommodate the substances needed for the cultivation process.
  • the cultivation chamber 10 is also connected to the air inlet 60.
  • the air inlet 60 may be a section of a supple bag divided into a conduit to pass air into the bag, as shown in FIGURE 1.
  • the air inlet 60 may be a separate tube or other profile attached to allow the passage of air into the cultivation chamber 10.
  • the cultivation chamber 10 is therefore in fluid communication with the ambient space or with a connected air source through the inlet air filter 61.
  • the inlet air filter 61 is connected to the cultivation chamber 10 via a channel 62, which leads the air to the cultivation chamber 10 through an inlet aeration port 11.
  • the inlet aeration port 11 is located at the bottom of the cultivation chamber 10.
  • the inlet aeration port 11 may be provided by making a portion of the seam defining the cultivation chamber 10, i.e. the bottom portion, discontinuous so as to arrange small openings being permeable to air but impermeable to the cell mass inside the cultivation chamber 10. Accordingly, the bottom seam of the cultivation chamber 10 is perforated for aeration.
  • the seam separating the cultivation chamber 10 from the channel 62 could alternatively or additionally be perforated.
  • the channel 62 is separated from the cultivation chamber 10 with a solid seam and shaped to extend under the cultivation chamber 10 along its entire length to ensure sufficient air supply to the entire biomass being cultivated including the bottom part of the mass.
  • the cultivation unit 100 also has a first auxiliary chamber 20, which is or can be separated from the cultivation chamber 10.
  • the purpose of the first auxiliary chamber 20 is to include a substance needed to cultivate the cells in the cultivation chamber 10.
  • the cultivation chamber 10 could initially contain the inoculum and the first auxiliary chamber 20 could include a nutrient for the inoculum, or vice versa.
  • the inoculum and nutrient are kept separate before the cultivation process. Indeed, it is particularly preferable to keep the inoculum and nutrient in a dehydrated or dry condition before cultivation.
  • the cultivation and first auxiliary chamber 10, 20 should be kept separate before cultivation.
  • the cultivation and first auxiliary chambers 10, 20 are initially separated from each other.
  • the chambers may be formed into the bag by plastic welding a seam connecting the sheet materials so as to define to sub-volumes of the bag. There should, however, be an opening in the seam to allow flow between the sub- volumes in a controlled manner.
  • a convertible passageway able to be converted from a closed state to an open state is therefore needed to connect the chambers 10, 20 to each other.
  • fluid flow between the cultivation chamber 10 and the first auxiliary chamber 20 is prevented in the closed state of the passageway 22 and enabled only in the open state.
  • Such a passageway 22 may be provided in many different ways. According to one option, the convertible passageway 22 is constructed as floodgate.
  • the floodgate may be set up as a sacrificial wall, separating the cultivation and first auxiliary chambers 10, 20 from each other in the closed state and being configured to be opened so as to enable fluid flow between the chambers 10, 20 in an open state.
  • the wall may be made by connecting the two sheet layers of the raw material of the bag into a brittle or breakable seam, which may be broken. In a broken state, the two layers of the sheet material will be separated thus allowing for a fluid flow between the cultivation and first auxiliary chamber 10, 20.
  • a sacrificial wall would be an irreversible floodgate.
  • the floodgate can be set up as reversible, whereby the two layers of sheet material would be separated from each other but perforated such to allow for a wall acting as the floodgate to be slid across to enable fluid flow.
  • Such a construction would, however, require fine sealing between the floodgate and the layers of sheet material.
  • the passageway 22 is constructed as two- component inter-engagement member.
  • the inter-engagement member can include two mutually fitting components provided to the superposed layers of sheet material.
  • One commercial option would be to use a so called MiniGrip® attachment.
  • the components are preferably configured to be manipulated from outside the cultivation unit 100 so as to fit each other in the closed state for separating the cultivation and first auxiliary chambers 10, 20 from each other, and to disengage from each other in the open state so as to enable fluid flow between the chambers 10, 20.
  • the passageway contains an external divider including a female piece, which has a recess for receiving the bag, which in turn is supple enough to conform to the shape of the recess.
  • the external divider also has a male piece designed to snap into the recess of the female piece and onto the bag and in doing so pressing the bag there between so as to block fluid flow in the back across the external divider.
  • the female piece is designed to be resilient enough to elastically deform upon insertion of the male piece, whereby the internal spring force will keep the female piece tightly on the male piece.
  • the male piece is removed from the female piece, whereby the pressure keeping the layers of sheet material against each other is removed and the substance kept separately in the cultivation and first auxiliary chambers 10, 20 may be brought into contact with each other.
  • the cultivation chamber 10 is connected to a water source via the water inlet 50.
  • the water inlet 50 is constructed to feed water into the cultivation chamber 10 through the first auxiliary chamber 20.
  • the water inlet 50 may be a section of a supple bag divided into a conduit to pass water into the bag, namely to the cultivation chamber 10, as shown in FIGURE 1.
  • the water inlet 50 may be a separate tube or other profile attached to allow the passage of water into the cultivation chamber 10. It is to be pointed out that water is only fed to the cultivation chamber 10 during the cultivation process.
  • the water inlet 50 is particularly designed to flush the contents of the first auxiliary chamber 20 into the cultivation chamber 10 when the passageway 22 is open.
  • the contact point between the first auxiliary chamber 20 and the water inlet is provided with a convertible passageway 21, which in a closed state prevents water from entering the first auxiliary chamber 20 but enables water flow in an opened state.
  • the inlet passageway 21 of the first auxiliary chamber 20 may be constructed similarly to outlet passageway 22.
  • the water inlet 50 comprises a channel 53 running from the sterile filter 52 past the first auxiliary chamber 20, the inlet passageway 21 of which is tapped into the channel 53. The remaining portion of the channel 53 is discussed here after.
  • the cultivation unit may also include more auxiliary chambers connected to the cultivation chamber similarly to the first auxiliary chamber 20.
  • the cultivation unit also includes a second and third auxiliary chamber 30, 40 connected to the cultivation chamber 10 via passageways 32, 42, respectfully.
  • the second and third auxiliary chambers 30, 40 are tapped into the channel 53 of the water inlet 50 similarly to the first auxiliary chamber 20 via inlet passageways 31, 41 , respectively.
  • the auxiliary chambers 20, 30, 40 are connected in parallel to the channel 53 of the water inlet 50.
  • the auxiliary chambers 20, 30, 40 may contain supplements to the inoculum contained in the cultivation chamber 10 that may be introduced to the cultivation chamber 10 in several stages. The use of the cultivation unit 100 will be discussed in greater detail here after.
  • FIGURE 1 also shows further connections of the cultivation chamber 10.
  • the air inlet 60 supplies air to the cultivation chamber 10 from below.
  • the air is lead out of the cultivation chamber 10 through an outlet aeration port 12, which can have a similar structure to the inlet aeration port 11.
  • the outlet aeration port 12 is designed to exhaust excess gases being produced by the cultivation process through an exhaust air port 70.
  • the outlet aeration port 12 is connected to an exhaust air condenser 72, which prevents excess evaporation of moisture during cultivation.
  • the exhaust air condenser 72 can be, for example a metal body cooled by Peltier element.
  • the second stage of the exhaust air port 70 is an exhaust air filter 71 connected to the exhaust air condenser 72 for providing a sterile barrier between the cultivation volume and the outside environment.
  • the exhaust air filter 71 may be a similar construction as the inlet air filter 61, for example.
  • the bottom of the cultivation chamber 10 includes an outlet 13 for the cultivated bio mass.
  • the outlet 13 may simply be an opening equipped with a convertible passageway, such as one described above.
  • the outlet 13 will preferably lead to a harvest chamber 80 located below the cultivation chamber 10 in the illustrated embodiment.
  • the harvest chamber 80 gathers the biomass produced in the cultivation chamber 10 via the outlet 13 and distributes the biomass to be collected to several collecting containers.
  • a collector 90 is provided to the harvest chamber 80 for collecting the ready product there from. According to the embodiment of FIGURE 1, the collector 90 comprises several collecting chambers, namely seven collecting chambers 91 - 97, which are connected to the harvest chamber 80 via a particular feeder channel 82.
  • the feeder channel 82 may simply be the bottom section of the harvest chamber 80 separated by a filter 81 for enabling a flush of the harvested product.
  • the cultivation unit 100 is constructed as a supple bag
  • the cultivated biomass may be pushed into the collecting containers 91 - 97 by applying pressure to the harvest chamber 80 by hand, for example.
  • the channel 53 of the water inlet 50 leads water to the feeder channel 82 for washing the product. Accordingly, it is preferable to equip the connection between the water channel 53 and the feeder channel 82 with a convertible passage way, such as on described above.
  • the collecting containers 91 - 97 connected to the feeder channels 82 may be bags made from the same material as the rest of the cultivation unit 100 but such seamed as to be separated from one another and from the feeder channel 82.
  • the seam surrounding the collecting containers 91 - 97 may therefore be made strong enough to keep the collecting containers 91 - 97 attached during normal use but weak enough to break under fatigue.
  • a cultivation unit 100 such as that shown in FIGURE 1 may be used as part of a cultivating kit produced as a self-contained source of biomass and intended to be use by amateurs in a non- laboratory environment.
  • the kit includes a cultivation unit and the ingredients for producing biomass except for water and air, which is to be sourced from said non- laboratory environment, such as tap water and ambient air.
  • the cultivation unit may be single-use unit, which is to be discarded after use, or it may be returned to the seller after use for replenishment depending on the structure of the cultivation unit.
  • the ingredients are stored in the cultivation unit separately in different chambers so as to prevent the cultivation process from starting during storage.
  • the ingredients are dry, which means that the dry weight content of the ingredients is 25 weight per cent or more, preferably 30 weight per cent or more.
  • the ingredients of the kit include an inoculum for cultivating cells, preferably edible cells, particularly plant cells.
  • suitable inocula include: arctic bramble (Rubus arcticus): several raspberry (Rubus idaeus),
  • strawberry Fragaria x ananasa
  • two cloudberry (Rubus chamaemorus), lines, stone berry (Rubus saxatilis), bilberry (Vaccinium myrtillus), cranberry (Vaccinium oxycoccos), sesame (Sesamum indicum), lingonberry (Vaccinium vitis-idaea), cotton (Gossypium spp.), crowberry (Empetrum nigrum), flax (Linum usitatissimum), rowanberry (Sorbus aucuparia), hazel (Corylus avellana), birch cells (Betula pendula), beech (Fagus sylvatica), honeysuckle (Lonicera caerulea var. parsnip (Pastinaca sativa),
  • spp. Phyllostachys pubescens, dandelion (Taraxacum officinale), Dendrocalamus latiflorus), taro (Colocasia esculenta), seakale (Crambe maritima), sago palm (Metroxylon sagu), cardoon (Cynara cardunculus), jackfruit (Artocarpus heterophyllus), rhubarb (Rheum rhabarbarum), sugar maple (Acer saccharum), garden sorrel (Rumex rugosus, R.
  • New Zealand spinach Tetragonia chick pea (Cicer arietinum)
  • Raphanus spp. Eruca vesicaria
  • mulberry (Morus alba, M. nigra),
  • liquorice (Glycyrrhiza glabra), litchi (Litchi chinensis, Nephelium
  • Cornelian cherry (Cornus mas), purslane (Portulaca oleracea), sea buckthorn (Hippophae rhamnoides), borage (Borago officinalis), elder (Sambucus niger), hyssop (Hyssopus officinalis),
  • juniper Juniperus communis
  • clove tree Syzygium aromaticum
  • Algae such as Chlorella sorokiniana and caraway (Carum carvi), Chlorella vulgaris, and coriander (Coriandrum sativum), Cyanobacterium, such as Arthrospira platensis and Arthrospira maxima.
  • kit may also be set up for cultivating a host of different biological matter including bacteria, yeast, fungus, mould, etc. by including an appropriate inoculum in the cultivation unit 100.
  • the inoculum may be included in the cultivation chamber 10 or the inoculum may be contained in one of the auxiliary chambers 20 - 40.
  • the ingredients also include a nutrient contained in the same chamber as the inoculum or preferably in a separate chamber, where from the nutrient may be introduced to the inoculum for cultivation by opening the separating passageway.
  • the inoculum may be contained in the cultivation chamber 10 and the nutrient may be contained in the first auxiliary chamber 20, whereby the two ingredients may be mixed by converting the passageways 21, 22 to an open state thus enabling water flow to the first auxiliary chamber 20.
  • the water flowing through the first auxiliary chamber 20 flushes the nutrient to the cultivation chamber 10 and the cultivation process may begin.
  • the cultivation chamber 10 may receive air from the air inlet 60 constantly or upon connecting the air inlet to an external pump (not shown). Naturally, the water inlet 50 must be connected to a water source, such as a tap, before use.
  • the kit may also include other ingredients, which are preferably kept separate from the above-mentioned ingredients before actively introduced to one another.
  • the kit may include a second nutrient contained originally in a separate chamber, such as the second auxiliary chamber 30. Similarly to the first nutrient, the second nutrient may be flushed into the cultivation chamber 10 by converting the inlet and outlet passageways 31, 32 into an open state thus allowing water to flow through the second auxiliary chamber 30.
  • the second nutrient may be introduced to the cultivation process later than the first nutrient.
  • the sugar (carbon source) concentration in the cultivation may not be very high (such as >200 g/L) since it may inhibit growth of the cells.
  • the same may apply to other nutrients as well such as ammonium (nitrogen source).
  • ammonium nitrogen source
  • additional foreseeable ingredients include additional formulations, which may be used in later stages of the cultivation process.
  • additional formulations may be included in yet further chambers, such as the third auxiliary chamber 40.
  • the additional formulation may take the form of substances regulating the flavor or texture (e.g. viscosity) of the produced biomass.
  • Exemplary additional formulations include natural or synthetic flavours, fragrances, pigments (colours) and/or their respective precursors for example: flavor/fragrance vanillin; flavor precursor betuligenol; pigment anthocyanin, carotenoid etc.
  • additional formulations will be flushed from the third auxiliary chamber 40 to the cultivation chamber 10.
  • the mass is harvested into the harvesting chamber 80 by opening the outlet 13 of the cultivation chamber 10.
  • the product is distributed along the width of the harvesting chamber 80 by pressing the supple bag by hand or by shaking the unit or by a large chamber-sized actuator.
  • the filter 81 will perform a final cleansing to the biomass before it is collected to the collector 90, such as a series of collecting containers 91 - 97, which may then be removed from the unit for delivering the biomass for consumption.
  • FIGURES 3 A to 3C show as an exemplary fashion the passageway 22 between the cultivation chamber 10 and the first auxiliary chamber 20.
  • the passageway 22 may take the form of an actuator comprising two cooperating components, the operation of which is more apparent in FIGURE 3 A.
  • the first component 22A is arranged on one side of the cultivation unit 100 and the second component 22B is arranged on the other side of the cultivation unit 100.
  • the first component 22A of the actuator includes a plunger, which is configured to be pushed from a closed state as shown in FIGURE 3B into an open state (not shown).
  • the second component is configured to connect to the first component 22A so as to clamp the cultivation unit 100 there between.
  • the clamping brings the two film layers in tight contact with one another thus preventing flow through the actuator.
  • the push of the plunger from the closed state into the open state makes the plunger to penetrate the cultivation unit, or vice versa.
  • the plunger therefore may act as a valve.
  • the actuator may include a biasing spring biasing the actuator toward the closed state. Such valves are known per se in the medical field.
  • the actuator may operate in response to a magnetic, electromagnetic, pneumatic or manual input.
  • FIGURE 1 may be varied in to simplify or otherwise adapt the cultivation unit to a particular purpose.
  • a cultivation unit is provided in a simplified modification from the cultivation unit of FIGURE 1.
  • the cultivation unit comprises only a cultivation chamber 10 and an isolated first auxiliary chamber 20.
  • the air inlet 60, water inlet 50, and exhaust air port 70 differ from those of the embodiment of FIGURE 1 in that they are constructed as tubes having external filters 51, 61, 71 attached to the tubes.
  • the tubes have been attached to the cultivation unit by adhesive connection or the tubes may have been sealed during the lamination of the parallel layers of the sheet forming the bag.
  • the filters 51, 61 , 71 form part of the sterile boundary.
  • the filters 51, 61, 71 are therefore the contact point of the cultivation unit to external supplies of air and water and to the ambience.
  • the collector shown in FIGURE 1 has been replaced by an outlet 98 which may take the form of a tube or other protruding volume.
  • the outlet 98 is preferably sealed by means of a cap 99 or other seal.
  • the aeration port of FIGURE 1 may be replaced by a sloping bottom end of the cultivation chamber 10.
  • the bottom end of the cultivation chamber 10 is made to slope towards the bottom end of the channel 62 of the air inlet 60 so as to feed air to the bottom-most part of the cultivation chamber 10.
  • FIGURE 5 A further variant is shown in FIGURE 6, wherein the cultivation unit of
  • FIGURE 5 is modified by replacing the inlets and air exhaust port of FIGURE 4 with those of FIGURE 1. Also, a collector 90 is added between the outlet 98 and the cultivation chamber 10 to provide for a larger volume.
  • the cultivation unit or kit as the ones described above may be part of a cultivation system including also a dock including the necessary framework for operating the cultivation unit.
  • the cultivation system may include dock for one or more cultivation units.
  • the cultivation unit and the dock preferably contain a standardized interface for connecting the two with a simple motion.
  • the dock may include, for example, ready water and/or air inlets preferably equipped with respective pumps for feeding water and/or air into the bag, automated manipulators for operating the passageways, particularly the actuated passageways, as well as an illuminator for providing sufficient light to the cultivation chamber in an appropriate wavelength.
  • the dock may also be equipped with sensors, such as absorbance, image recognition, spectrometers, etc., for monitoring the progress of the cultivation.
  • the dock also preferably includes a user interface for controlling the system.
  • the user interface may be, for example, a network connection enabling remote control through web-based services, apps, etc.

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Abstract

L'invention concerne également une unité de culture autonome et facile à utiliser pour amateurs. L'unité comprend une limite stérile qui isole l'unité de culture des contaminants externes, une chambre de culture (10), une entrée d'eau (50) configurée pour alimenter en eau la chambre de culture (10), et une entrée d'air (60) configurée pour alimenter en air la chambre de culture (10). Une première chambre auxiliaire (20) est reliée à la chambre de culture (10) par l'intermédiaire d'un passage (22), qui peut être manipulé d'un état fermé à un état ouvert de façon à introduire une substance contenue dans la chambre de culture (10) et une substance contenue dans la première chambre auxiliaire (20) d'une chambre à l'autre.
PCT/FI2017/050717 2016-10-14 2017-10-12 Unité de culture et kit pour cultiver des cellules Ceased WO2018069578A1 (fr)

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FI20165786 2016-10-14
FI20165786 2016-10-14

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025066448A1 (fr) * 2023-09-25 2025-04-03 成都赛恩吉诺生物科技有限公司 Système de dissociation tissulaire et procédé de dissociation tissulaire

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