WO2024254620A3 - Protease for diagnostics - Google Patents

Protease for diagnostics Download PDF

Info

Publication number
WO2024254620A3
WO2024254620A3 PCT/US2024/040585 US2024040585W WO2024254620A3 WO 2024254620 A3 WO2024254620 A3 WO 2024254620A3 US 2024040585 W US2024040585 W US 2024040585W WO 2024254620 A3 WO2024254620 A3 WO 2024254620A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
diagnostics
kits
buffers
extracting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/040585
Other languages
French (fr)
Other versions
WO2024254620A2 (en
Inventor
Maria Tang
Stephanie MANTON
Andrea Sabine ILG
Lars Kobberoee Skov
Swapnil Surendra Mohile
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to PCT/US2024/040585 priority Critical patent/WO2024254620A2/en
Publication of WO2024254620A2 publication Critical patent/WO2024254620A2/en
Publication of WO2024254620A3 publication Critical patent/WO2024254620A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention pertains to methods, kits, and buffers comprising a protease for hydrolyzing proteins and extracting a non-proteinaceous component from biological samples.
PCT/US2024/040585 2024-08-01 2024-08-01 Protease for diagnostics Pending WO2024254620A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2024/040585 WO2024254620A2 (en) 2024-08-01 2024-08-01 Protease for diagnostics

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2024/040585 WO2024254620A2 (en) 2024-08-01 2024-08-01 Protease for diagnostics

Publications (2)

Publication Number Publication Date
WO2024254620A2 WO2024254620A2 (en) 2024-12-12
WO2024254620A3 true WO2024254620A3 (en) 2025-05-01

Family

ID=92538870

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/040585 Pending WO2024254620A2 (en) 2024-08-01 2024-08-01 Protease for diagnostics

Country Status (1)

Country Link
WO (1) WO2024254620A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1201753A1 (en) * 2000-10-31 2002-05-02 Boehringer Mannheim Gmbh Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
EP1201752A1 (en) * 2000-10-31 2002-05-02 Roche Diagnostics GmbH Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US6727067B2 (en) * 2000-10-31 2004-04-27 Roche Molecular Systems, Inc. Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
CN102115739B (en) * 2009-12-31 2014-07-16 财团法人工业技术研究院 Method, reagent and kit for isolating nucleic acid
KR102247419B1 (en) * 2020-03-24 2021-05-03 정지원 Buffer composition and analysis method using the same
KR102580940B1 (en) * 2021-08-19 2023-09-20 바이오메디팜 주식회사 Extraction Method of high purity Poly Deoxy Ribo Nucleotide from meats of Salmonid fish

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK263584D0 (en) 1984-05-29 1984-05-29 Novo Industri As ENZYMOUS GRANULATES USED AS DETERGENT ADDITIVES
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5879920A (en) 1991-10-07 1999-03-09 Genencor International, Inc. Coated enzyme-containing granule
AU677166B2 (en) 1991-10-07 1997-04-17 Genencor International, Inc. Coated enzyme containing granule
DE4343591A1 (en) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Process for the evolutionary design and synthesis of functional polymers based on shape elements and shape codes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
EP1092007B1 (en) 1998-06-30 2003-11-26 Novozymes A/S A new improved enzyme containing granule
EP1224272B1 (en) 1999-10-01 2005-12-07 Novozymes A/S Spray dried enzyme product
NZ589121A (en) 2008-06-09 2012-10-26 Danisco Us Inc Recovery of insoluble enzyme from fermentation broth and formulation of insoluble enzyme
WO2015028567A1 (en) 2013-08-28 2015-03-05 Novozymes A/S Enzyme granule with fluorescent whitening agent
EP3122859B1 (en) 2014-03-25 2019-08-28 Novozymes A/S Improved microencapsulation using small amines
EP3180429B1 (en) 2014-08-11 2020-03-04 Novozymes A/S Detergents and compositions with enzymatic polymer particles
US20200181542A1 (en) 2017-06-30 2020-06-11 Novozymes A/S Enzyme Slurry Composition

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1201753A1 (en) * 2000-10-31 2002-05-02 Boehringer Mannheim Gmbh Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
EP1201752A1 (en) * 2000-10-31 2002-05-02 Roche Diagnostics GmbH Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US6727067B2 (en) * 2000-10-31 2004-04-27 Roche Molecular Systems, Inc. Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
CN102115739B (en) * 2009-12-31 2014-07-16 财团法人工业技术研究院 Method, reagent and kit for isolating nucleic acid
KR102247419B1 (en) * 2020-03-24 2021-05-03 정지원 Buffer composition and analysis method using the same
KR102580940B1 (en) * 2021-08-19 2023-09-20 바이오메디팜 주식회사 Extraction Method of high purity Poly Deoxy Ribo Nucleotide from meats of Salmonid fish

Also Published As

Publication number Publication date
WO2024254620A2 (en) 2024-12-12

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