WO2024254620A3 - Protéase pour diagnostic - Google Patents

Protéase pour diagnostic Download PDF

Info

Publication number
WO2024254620A3
WO2024254620A3 PCT/US2024/040585 US2024040585W WO2024254620A3 WO 2024254620 A3 WO2024254620 A3 WO 2024254620A3 US 2024040585 W US2024040585 W US 2024040585W WO 2024254620 A3 WO2024254620 A3 WO 2024254620A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
diagnostics
kits
buffers
extracting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/040585
Other languages
English (en)
Other versions
WO2024254620A2 (fr
Inventor
Maria Tang
Stephanie MANTON
Andrea Sabine ILG
Lars Kobberoee Skov
Swapnil Surendra Mohile
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to PCT/US2024/040585 priority Critical patent/WO2024254620A2/fr
Publication of WO2024254620A2 publication Critical patent/WO2024254620A2/fr
Publication of WO2024254620A3 publication Critical patent/WO2024254620A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés, des kits et des tampons comprenant une protéase pour hydrolyser des protéines et extraire un composant non protéique d'échantillons biologiques.
PCT/US2024/040585 2024-08-01 2024-08-01 Protéase pour diagnostic Pending WO2024254620A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2024/040585 WO2024254620A2 (fr) 2024-08-01 2024-08-01 Protéase pour diagnostic

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2024/040585 WO2024254620A2 (fr) 2024-08-01 2024-08-01 Protéase pour diagnostic

Publications (2)

Publication Number Publication Date
WO2024254620A2 WO2024254620A2 (fr) 2024-12-12
WO2024254620A3 true WO2024254620A3 (fr) 2025-05-01

Family

ID=92538870

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/040585 Pending WO2024254620A2 (fr) 2024-08-01 2024-08-01 Protéase pour diagnostic

Country Status (1)

Country Link
WO (1) WO2024254620A2 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1201753A1 (fr) * 2000-10-31 2002-05-02 Boehringer Mannheim Gmbh Procédé pour l'analyse de composants non-protéiques en utilisant une protéase provénante d'une souche de Bacillus
EP1201752A1 (fr) * 2000-10-31 2002-05-02 Roche Diagnostics GmbH Methode pour l'analyse de composants non protéiques utilisant une protéase derivée d'une souche de Bacillus
US6727067B2 (en) * 2000-10-31 2004-04-27 Roche Molecular Systems, Inc. Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
CN102115739B (zh) * 2009-12-31 2014-07-16 财团法人工业技术研究院 分离核酸的方法、试剂及套组
KR102247419B1 (ko) * 2020-03-24 2021-05-03 정지원 버퍼 조성물 및 이를 이용한 분석방법
KR102580940B1 (ko) * 2021-08-19 2023-09-20 바이오메디팜 주식회사 연어과 어류의 살로부터 고순도 디엔에이의 추출 방법

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
US5879920A (en) 1991-10-07 1999-03-09 Genencor International, Inc. Coated enzyme-containing granule
ES2167319T3 (es) 1991-10-07 2002-05-16 Genencor Int Granulo que contiene una enzima revestida.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE69913146T2 (de) 1998-06-30 2004-09-16 Novozymes A/S Neues, verbessertes, enzymhaltiges Granulat
AU7405200A (en) 1999-10-01 2001-05-10 Novozymes A/S Spray dried enzyme product
RU2010154462A (ru) 2008-06-09 2012-07-20 ДАНИСКО ЮЭс ИНК. (US) Выделение нерастворимого фермента из ферментного бульона и составление нерастворимого фермента
US20160177240A1 (en) 2013-08-28 2016-06-23 Novozymes A/S Enzyme Granule with Fluorescent Whitening Agent
EP3122859B1 (fr) 2014-03-25 2019-08-28 Novozymes A/S Micro-encapsulation faisant intervenir de petites amines
CN106414734B (zh) 2014-08-11 2020-11-03 诺维信公司 具有酶促聚合物颗粒的洗涤剂和组合物
CN111108183A (zh) 2017-06-30 2020-05-05 诺维信公司 酶浆液组合物

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1201753A1 (fr) * 2000-10-31 2002-05-02 Boehringer Mannheim Gmbh Procédé pour l'analyse de composants non-protéiques en utilisant une protéase provénante d'une souche de Bacillus
EP1201752A1 (fr) * 2000-10-31 2002-05-02 Roche Diagnostics GmbH Methode pour l'analyse de composants non protéiques utilisant une protéase derivée d'une souche de Bacillus
US6727067B2 (en) * 2000-10-31 2004-04-27 Roche Molecular Systems, Inc. Methods for the analysis of non-proteinaceous components using a protease from a Bacillus strain
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
CN102115739B (zh) * 2009-12-31 2014-07-16 财团法人工业技术研究院 分离核酸的方法、试剂及套组
KR102247419B1 (ko) * 2020-03-24 2021-05-03 정지원 버퍼 조성물 및 이를 이용한 분석방법
KR102580940B1 (ko) * 2021-08-19 2023-09-20 바이오메디팜 주식회사 연어과 어류의 살로부터 고순도 디엔에이의 추출 방법

Also Published As

Publication number Publication date
WO2024254620A2 (fr) 2024-12-12

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