WO2024258979A3 - Method and system of multi-modal sub-cellular segmentation - Google Patents

Method and system of multi-modal sub-cellular segmentation Download PDF

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Publication number
WO2024258979A3
WO2024258979A3 PCT/US2024/033630 US2024033630W WO2024258979A3 WO 2024258979 A3 WO2024258979 A3 WO 2024258979A3 US 2024033630 W US2024033630 W US 2024033630W WO 2024258979 A3 WO2024258979 A3 WO 2024258979A3
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segmentation
cleavable
photo
systems
modal
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French (fr)
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WO2024258979A2 (en
Inventor
Aster WARDHANI
Lidan WU
Dwayne Dunaway
Winnie Wing-Yin LEUNG
Sanghamithra Korukonda
David Ross
John E. Chandler
Carl Brown
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Bruker Spatial Biology Inc
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Bruker Spatial Biology Inc
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Priority to CN202480039614.2A priority Critical patent/CN121569321A/en
Priority to AU2024304188A priority patent/AU2024304188A1/en
Priority to KR1020267000485A priority patent/KR20260022393A/en
Priority to EP24824082.2A priority patent/EP4728471A2/en
Publication of WO2024258979A2 publication Critical patent/WO2024258979A2/en
Publication of WO2024258979A3 publication Critical patent/WO2024258979A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/73—Deblurring; Sharpening
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H30/00—ICT specially adapted for the handling or processing of medical images
    • G16H30/40—ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/20—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20016—Hierarchical, coarse-to-fine, multiscale or multiresolution image processing; Pyramid transform
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20081—Training; Learning
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20084—Artificial neural networks [ANN]
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30096—Tumor; Lesion
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Primary Health Care (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Databases & Information Systems (AREA)
  • Data Mining & Analysis (AREA)
  • Radiology & Medical Imaging (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Described herein are systems and methods of multi-modal subcellular segmentation using photo-cleavable biomarkers and/or transcriptomic readout density maps. The systems and methods provide an improvement in cell segmentation accuracy for nuclei, cytoplasm and membrane regions through the use of optical and bleaching corrections from multiple sources of photo-cleavable morphology markers in combination with high-quality 3D images acquired with high-dynamic range scanning, and spatial transcriptomic readout density maps.
PCT/US2024/033630 2023-06-13 2024-06-12 Method and system of multi-modal sub-cellular segmentation Ceased WO2024258979A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN202480039614.2A CN121569321A (en) 2023-06-13 2024-06-12 Multi-mode subcellular segmentation method and system
AU2024304188A AU2024304188A1 (en) 2023-06-13 2024-06-12 Method and system of multi-modal sub-cellular segmentation
KR1020267000485A KR20260022393A (en) 2023-06-13 2024-06-12 Multi-modal intracellular segmentation method and system
EP24824082.2A EP4728471A2 (en) 2023-06-13 2024-06-12 Method and system of multi-modal sub-cellular segmentation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363507824P 2023-06-13 2023-06-13
US63/507,824 2023-06-13

Publications (2)

Publication Number Publication Date
WO2024258979A2 WO2024258979A2 (en) 2024-12-19
WO2024258979A3 true WO2024258979A3 (en) 2025-02-20

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PCT/US2024/033630 Ceased WO2024258979A2 (en) 2023-06-13 2024-06-12 Method and system of multi-modal sub-cellular segmentation

Country Status (5)

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EP (1) EP4728471A2 (en)
KR (1) KR20260022393A (en)
CN (1) CN121569321A (en)
AU (1) AU2024304188A1 (en)
WO (1) WO2024258979A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120125582B (en) * 2025-05-13 2026-04-10 大熊(天津)医疗科技有限公司 A medical cell detection method
CN120178493B (en) * 2025-05-22 2025-08-15 北京心联光电科技有限公司 Automatic focusing method and system for cells interfered by fluorescent microspheres in hydrogel

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140314299A1 (en) * 2013-04-17 2014-10-23 General Electric Company System and Method for Multiplexed Biomarker Quantitation Using Single Cell Segmentation on Sequentially Stained Tissue
WO2021076928A1 (en) * 2019-10-18 2021-04-22 Nanostring Technologies, Inc. Systems and methods for spatial mapping of expression profiling
US20230100912A1 (en) * 2020-03-05 2023-03-30 Insightec, Ltd. Differential prediction of aberration corrections for ultrasound therapy

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140314299A1 (en) * 2013-04-17 2014-10-23 General Electric Company System and Method for Multiplexed Biomarker Quantitation Using Single Cell Segmentation on Sequentially Stained Tissue
WO2021076928A1 (en) * 2019-10-18 2021-04-22 Nanostring Technologies, Inc. Systems and methods for spatial mapping of expression profiling
US20230100912A1 (en) * 2020-03-05 2023-03-30 Insightec, Ltd. Differential prediction of aberration corrections for ultrasound therapy

Also Published As

Publication number Publication date
KR20260022393A (en) 2026-02-19
WO2024258979A2 (en) 2024-12-19
AU2024304188A1 (en) 2025-11-13
EP4728471A2 (en) 2026-04-22
CN121569321A (en) 2026-02-24

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