WO2024259415A3 - Universal lamp for capturing non-cognate nucleic acids in lamp concatemers - Google Patents

Universal lamp for capturing non-cognate nucleic acids in lamp concatemers Download PDF

Info

Publication number
WO2024259415A3
WO2024259415A3 PCT/US2024/034311 US2024034311W WO2024259415A3 WO 2024259415 A3 WO2024259415 A3 WO 2024259415A3 US 2024034311 W US2024034311 W US 2024034311W WO 2024259415 A3 WO2024259415 A3 WO 2024259415A3
Authority
WO
WIPO (PCT)
Prior art keywords
lamp
concatemers
universal
nucleic acids
cognate nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/034311
Other languages
French (fr)
Other versions
WO2024259415A2 (en
Inventor
Andrew Ellington
Sanchita BHADRA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
University of Texas at Austin
Original Assignee
University of Texas System
University of Texas at Austin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System, University of Texas at Austin filed Critical University of Texas System
Publication of WO2024259415A2 publication Critical patent/WO2024259415A2/en
Publication of WO2024259415A3 publication Critical patent/WO2024259415A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Universal LAMP can be used to capture target nucleic acid sequences within LAMP concatemers without having to design new LAMP primers for each target. These universal LAMP primers can be used in a variety of amplification techniques and can be included in a kit.
PCT/US2024/034311 2023-06-16 2024-06-17 Universal lamp for capturing non-cognate nucleic acids in lamp concatemers Ceased WO2024259415A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508633P 2023-06-16 2023-06-16
US63/508,633 2023-06-16

Publications (2)

Publication Number Publication Date
WO2024259415A2 WO2024259415A2 (en) 2024-12-19
WO2024259415A3 true WO2024259415A3 (en) 2025-04-03

Family

ID=93852690

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/034311 Ceased WO2024259415A2 (en) 2023-06-16 2024-06-17 Universal lamp for capturing non-cognate nucleic acids in lamp concatemers

Country Status (1)

Country Link
WO (1) WO2024259415A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150126382A1 (en) * 2012-05-14 2015-05-07 Peter Warthoe Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids
US20210403979A1 (en) * 2014-09-17 2021-12-30 Board Of Regents, The University Of Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150126382A1 (en) * 2012-05-14 2015-05-07 Peter Warthoe Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids
US20210403979A1 (en) * 2014-09-17 2021-12-30 Board Of Regents, The University Of Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Also Published As

Publication number Publication date
WO2024259415A2 (en) 2024-12-19

Similar Documents

Publication Publication Date Title
Wu et al. Versatile detection with CRISPR/Cas system from applications to challenges
US20090111705A1 (en) Selection of dna adaptor orientation by hybrid capture
US11827933B2 (en) Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element
NZ787446A (en) Compositions and methods for rna-encoded dna-replacement of alleles
WO2007149789A3 (en) Conversion of target specific amplification to universal sequencing
US7901890B2 (en) Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2024259415A3 (en) Universal lamp for capturing non-cognate nucleic acids in lamp concatemers
WO2018191702A3 (en) Methods of attaching adapters to sample nucleic acids
CN106282241A (en) The method obtaining knocking out the Brachydanio rerio of bmp2a gene by CRISPR/Cas9
EP2213741A3 (en) Compositions and methods of using a synthetic DNAse I
DK1836302T3 (en) Ligation-based RNA amplification
CN102286633B (en) Avian infectious bronchitis virus quick detection kit based on loop-mediated isothermal amplification technology and application method thereof
WO2016078096A1 (en) Method using bubble-shaped connector elements to construct sequencing library
WO2016058134A1 (en) Linker element and method of using same to construct sequencing library
EP0919121A4 (en) In vivo electroporation method for early animal embryo
EP4516899A3 (en) Nuclease having improved salt tolerance and/or temperature performance
ATE534731T1 (en) COMPOSITIONS AND METHODS FOR RANDOM MUTAGENESIS OF NUCLEIC ACIDS
EP3587593A3 (en) Compositions, methods and kits to detect herpes simplex virus nucleic acids
CN105255857A (en) A kind of extraction method of tea tree DNA
CN113817803B (en) A library construction method carrying modified small RNA and its application
WO2023244992A3 (en) Cas endonuclease and guide rna variants with improved efficiency
CN110468123B (en) Chlamydomonas reinhardtii Nuclear Genome PCR System
WO2023172988A3 (en) Bst polymerase variants
CN113025689A (en) Library construction method for modified small RNA and application thereof
Gong et al. Recent Progress in Regulating CRISPR-Cas9 System for Gene Editing

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24824343

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE