WO2024259415A3 - Lamp universelle pour capturer des acides nucléiques non apparentés dans des concatémères lamp - Google Patents

Lamp universelle pour capturer des acides nucléiques non apparentés dans des concatémères lamp Download PDF

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Publication number
WO2024259415A3
WO2024259415A3 PCT/US2024/034311 US2024034311W WO2024259415A3 WO 2024259415 A3 WO2024259415 A3 WO 2024259415A3 US 2024034311 W US2024034311 W US 2024034311W WO 2024259415 A3 WO2024259415 A3 WO 2024259415A3
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WO
WIPO (PCT)
Prior art keywords
lamp
concatemers
universal
nucleic acids
cognate nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/034311
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English (en)
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WO2024259415A2 (fr
Inventor
Andrew Ellington
Sanchita BHADRA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
University of Texas at Austin
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University of Texas System
University of Texas at Austin
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Application filed by University of Texas System, University of Texas at Austin filed Critical University of Texas System
Publication of WO2024259415A2 publication Critical patent/WO2024259415A2/fr
Publication of WO2024259415A3 publication Critical patent/WO2024259415A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Une LAMP universelle peut être utilisée pour capturer des séquences d'acides nucléiques cibles dans des concatémères LAMP sans avoir à concevoir de nouvelles amorces LAMP pour chaque cible. Ces amorces LAMP universelles peuvent être utilisées dans diverses techniques d'amplification et peuvent être incluses dans un kit.
PCT/US2024/034311 2023-06-16 2024-06-17 Lamp universelle pour capturer des acides nucléiques non apparentés dans des concatémères lamp Ceased WO2024259415A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508633P 2023-06-16 2023-06-16
US63/508,633 2023-06-16

Publications (2)

Publication Number Publication Date
WO2024259415A2 WO2024259415A2 (fr) 2024-12-19
WO2024259415A3 true WO2024259415A3 (fr) 2025-04-03

Family

ID=93852690

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/034311 Ceased WO2024259415A2 (fr) 2023-06-16 2024-06-17 Lamp universelle pour capturer des acides nucléiques non apparentés dans des concatémères lamp

Country Status (1)

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WO (1) WO2024259415A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150126382A1 (en) * 2012-05-14 2015-05-07 Peter Warthoe Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids
US20210403979A1 (en) * 2014-09-17 2021-12-30 Board Of Regents, The University Of Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150126382A1 (en) * 2012-05-14 2015-05-07 Peter Warthoe Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids
US20210403979A1 (en) * 2014-09-17 2021-12-30 Board Of Regents, The University Of Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Also Published As

Publication number Publication date
WO2024259415A2 (fr) 2024-12-19

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