WO2025093674A3 - Procédé d'expansion de cellules hépatiques - Google Patents

Procédé d'expansion de cellules hépatiques Download PDF

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Publication number
WO2025093674A3
WO2025093674A3 PCT/EP2024/080816 EP2024080816W WO2025093674A3 WO 2025093674 A3 WO2025093674 A3 WO 2025093674A3 EP 2024080816 W EP2024080816 W EP 2024080816W WO 2025093674 A3 WO2025093674 A3 WO 2025093674A3
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Prior art keywords
liver cells
expanding
expansion medium
expanding liver
proviso
Prior art date
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Pending
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PCT/EP2024/080816
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English (en)
Other versions
WO2025093674A2 (fr
Inventor
Meritxell Huch Ortega
Yohan Kim
Sagarika DAWKA
Robert ARNES BENITO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
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Publication of WO2025093674A2 publication Critical patent/WO2025093674A2/fr
Publication of WO2025093674A3 publication Critical patent/WO2025093674A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Physiology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé d'expansion de cellules hépatiques, le procédé comprenant (a) l'expansion des cellules hépatiques à l'intérieur d'une matrice extracellulaire dans un milieu d'expansion, le milieu d'expansion comprenant un substitut ou activateur de Wnt et un inhibiteur de la voie de signalisation Hippo, et de préférence à condition que le milieu d'expansion ne comprenne pas de nicotinamide.
PCT/EP2024/080816 2023-11-02 2024-10-31 Procédé d'expansion de cellules hépatiques Pending WO2025093674A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23207475.7 2023-11-02
EP23207475 2023-11-02

Publications (2)

Publication Number Publication Date
WO2025093674A2 WO2025093674A2 (fr) 2025-05-08
WO2025093674A3 true WO2025093674A3 (fr) 2025-06-12

Family

ID=88690149

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/080816 Pending WO2025093674A2 (fr) 2023-11-02 2024-10-31 Procédé d'expansion de cellules hépatiques

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WO (1) WO2025093674A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020109324A1 (fr) * 2018-11-26 2020-06-04 Koninklijke Nederlandse Akademie Van Wetenschappen Procédés d'expansion d'hépatocytes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US6080560A (en) 1994-07-25 2000-06-27 Monsanto Company Method for producing antibodies in plant cells
EP1892248A1 (fr) 2006-08-21 2008-02-27 Eidgenössische Technische Hochschule Zürich Protéines liantes spécifiques et de haute affinité comprenant des domaines SH3 de FYN kinase modifiés
EP2393917B1 (fr) 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Milieu de culture de cellules souches épithéliales et organoïdes comprenant lesdites cellules souches
EP2412800A1 (fr) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Organoïde du foie, ses utilisations et son procédé de culture pour l'obtenir
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
CA2949171A1 (fr) 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Procede de culture ameliore pour organoides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020109324A1 (fr) * 2018-11-26 2020-06-04 Koninklijke Nederlandse Akademie Van Wetenschappen Procédés d'expansion d'hépatocytes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FAN FUQIN ET AL: "Pharmacological targeting of kinases MST1 and MST2 augments tissue repair and regeneration", vol. 8, no. 352, 17 August 2016 (2016-08-17), pages 1 - 15, XP009542964, ISSN: 1946-6234, Retrieved from the Internet <URL:https://www.science.org/doi/10.1126/scitranslmed.aaf2304> [retrieved on 20240425], DOI: 10.1126/SCITRANSLMED.AAF2304 *
GREENBAUM LINDA E ET AL: "Clinical translation of liver regeneration therapies: A conceptual road map", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 175, 5 February 2020 (2020-02-05), XP086132997, ISSN: 0006-2952, [retrieved on 20200205], DOI: 10.1016/J.BCP.2020.113847 *
HU YIQING ET AL: "Liver organoid culture methods", vol. 13, no. 1, 1 November 2023 (2023-11-01), London, UK, XP093143437, ISSN: 2045-3701, Retrieved from the Internet <URL:https://link.springer.com/article/10.1186/s13578-023-01136-x/fulltext.html> [retrieved on 20240422], DOI: 10.1186/s13578-023-01136-x *
JORDAN H. DRISKILL: "The Hippo Pathway in Liver Homeostasis and Pathophysiology", vol. 16, no. 1, 24 January 2021 (2021-01-24), US, pages 299 - 322, XP093155344, ISSN: 1553-4006, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594752/pdf/nihms-1747101.pdf> [retrieved on 20240424], DOI: 10.1146/annurev-pathol-030420-105050 *
LAURA BROUTIER ET AL: "Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation", NATURE PROTOCOLS, vol. 11, no. 9, 25 August 2016 (2016-08-25), GB, pages 1724 - 1743, XP055534706, ISSN: 1754-2189, DOI: 10.1038/nprot.2016.097 *
LAURA BROUTIER ET AL: "Human primary liver cancer-derived organoid cultures for disease modeling and drug screening", NATURE MEDICINE(AUTHOR MANUSCRIPT ), vol. 23, no. 12, 13 November 2017 (2017-11-13), New York, pages 1424 - 1435, XP055534757, ISSN: 1078-8956, DOI: 10.1038/nm.4438 *

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