WO2025238109A2 - Procédé de réduction de la charge bactérienne sur ou dans une installation de climatisation - Google Patents

Procédé de réduction de la charge bactérienne sur ou dans une installation de climatisation

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Publication number
WO2025238109A2
WO2025238109A2 PCT/EP2025/063289 EP2025063289W WO2025238109A2 WO 2025238109 A2 WO2025238109 A2 WO 2025238109A2 EP 2025063289 W EP2025063289 W EP 2025063289W WO 2025238109 A2 WO2025238109 A2 WO 2025238109A2
Authority
WO
WIPO (PCT)
Prior art keywords
bacteriophages
air conditioning
component
conditioning system
bacteriophage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/063289
Other languages
German (de)
English (en)
Other versions
WO2025238109A3 (fr
Inventor
Klaus Hoppenheidt
Sabine Riha
Sandra Held
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bifa Umweltinstitut GmbH
Tunap GmbH and Co KG
Original Assignee
Bifa Umweltinstitut GmbH
Tunap GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bifa Umweltinstitut GmbH, Tunap GmbH and Co KG filed Critical Bifa Umweltinstitut GmbH
Publication of WO2025238109A2 publication Critical patent/WO2025238109A2/fr
Publication of WO2025238109A3 publication Critical patent/WO2025238109A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Disinfection or sterilisation of materials or objects, in general; Accessories therefor
    • A61L2/16—Disinfection or sterilisation of materials or objects, in general; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B60—VEHICLES IN GENERAL
    • B60H—ARRANGEMENTS OF HEATING, COOLING, VENTILATING OR OTHER AIR-TREATING DEVICES SPECIALLY ADAPTED FOR PASSENGER OR GOODS SPACES OF VEHICLES
    • B60H3/00—Other air-treating devices
    • B60H3/0085—Smell or pollution preventing arrangements
    • B60H3/0092—Smell or pollution preventing arrangements in the interior of the HVAC unit, e.g. by spraying substances inside the unit
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24—HEATING; RANGES; VENTILATING
    • F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
    • F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
    • F24F8/20—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation
    • F24F8/24—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation using sterilising media
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24—HEATING; RANGES; VENTILATING
    • F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
    • F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
    • F24F8/90—Cleaning of purification apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10—Apparatus features
    • A61L2202/17—Combination with washing or cleaning means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/10—Apparatus features
    • A61L2209/16—Connections to a HVAC unit
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10032—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof

Definitions

  • the present invention relates to a method for reducing the bacterial load on or in an air conditioning system, as well as various uses of bacteriophages or a component thereof. Furthermore, the present invention relates to certain deposited bacteriophages as such.
  • dirt particles and microorganisms often accumulate on the system's components after only a short time, especially directly on the evaporator and the pollen filter housing. These microorganisms use the dirt particles and airborne components as a food source and can form a stable biofilm. Heavy biofilm growth produces unpleasant odors that are clearly noticeable in the passenger compartment. This occurs particularly when air conditioning units are left unused for extended periods after the introduction of microbially digestible substances, especially in high humidity and mild temperatures.
  • An alcohol-based cleaner is sprayed directly onto the evaporator at high pressure using a pressure cup gun and a rigid probe. This removes dirt and deposits.
  • the high spray pressure flushes the evaporator and removes the biofilm formed by microorganisms. This system is currently the most effective system for cleaning air conditioning systems.
  • the foam is sprayed towards the evaporator using a probe, either through the air conditioning system's condensate drain or through the pollen filter box opening.
  • the foam is intended to coat the evaporator and thereby clean/disinfect it.
  • a cleaning agent is atomized using an ultrasonic device placed inside the vehicle.
  • the spray is then circulated through the air conditioning system via the recirculation setting and distributed throughout the interior.
  • ozone is produced from atmospheric oxygen and then introduced into the vehicle interior.
  • organic compounds such as bacteria and other germs, are broken down.
  • the escaping ozone of the Generators are hazardous to health and can cause lung damage and/or headaches, among other things, if inhaled.
  • Biocidal Products Regulation Biocidal Products Regulation
  • viruses can be combated by viruses, in particular by lysis and thus killing them.
  • viruses which are specialized to infect bacteria as host cells, are called bacteriophages and are classified into different groups according to their host specificity.
  • bacteriophages are only used in the medical, therapeutic, and food technology sectors. These applications primarily involve medically relevant bacteriophages, meaning bacteriophages that lyse pathogenic bacteria.
  • WO 2009/090097 A1 describes the use of bacteriophages in hygiene and care products, particularly for wound care.
  • WO 2010/090542 A2 This describes the therapeutic treatment of bacterial infections using specific bacteriophages.
  • bacteriophages are used to treat Pseudomonas infections.
  • DE 601 06 727 T2 discloses food packaging containing bacteriophages.
  • bacteriophages in air conditioning systems, such as those found in vehicles, has not yet been described to date.
  • bacteriophages represent a promising biological approach that can overcome the disadvantages of known chemical and environmentally harmful substances.
  • One object of the present invention is therefore to achieve a biological removal or at least reduction of bacteria in air conditioning systems without the disadvantages of the prior art due to chemical, environmentally harmful substances.
  • This problem is solved according to the invention by using bacteriophages or a component thereof to reduce the bacterial load on or in an air conditioning system, to prevent the formation of a biofilm or to remove a biofilm on or in an air conditioning system, and/or to prevent the formation of odors in an air conditioning system.
  • vehicle air conditioning systems primarily contain representatives of the two bacterial genera Sphingomonas spp. and Methylobacterium spp.
  • the inventors of the present invention Furthermore, the invention made it possible to isolate suitable bacteriophages with these bacterial species as host organisms and thus selectively combat the bacteria predominantly present in a vehicle air conditioning system. A long-term effect (depot effect) can also be achieved, along with good material compatibility.
  • the present invention relates accordingly to a method for reducing the bacterial load (or a method for removing, killing or reducing the number of bacteria, preventing the formation of a biofilm or removing a biofilm and/or preventing the formation of odors) on or in an air conditioning system, wherein the method comprises applying bacteriophages or a component thereof to one or more parts of the air conditioning system.
  • the present invention relates to the use of bacteriophages or a component thereof for reducing the bacterial load (for removing, killing or reducing the number of bacteria) on or in an air conditioning system.
  • the present invention relates to the use of bacteriophages or a component thereof to prevent the formation of a biofilm or to remove a biofilm on or in an air conditioning system.
  • the present invention relates to the use of bacteriophages or a component thereof to prevent the formation of (bacterially caused, unpleasant) odors in an air conditioning system.
  • the present invention relates to selected bacteriophages or a component thereof, comprising at least one from the group consisting of Bacteriophage SP-P_L_l_2022, filed under DSM 34460, and Bacteriophage SP-P_M_l_2022, filed under DSM 34461. Further tasks and advantages of embodiments of the present invention will become apparent from the following detailed description.
  • the present invention relates to a method for reducing the bacterial load on or in an air conditioning system.
  • reduction of bacterial load means in particular that the number of (live or active) bacteria is reduced, or that bacterial infestation is reduced or even prevented.
  • the bacteriophages used, or a component thereof, may exhibit antimicrobial activity or efficacy for this purpose.
  • an “antibacterial effect” or “antibacterial efficacy” is understood to mean the ability to kill bacteria or at least to inhibit their growth.
  • an "antibacterial effect” or “antibacterial efficacy” is understood to mean a bacteriostatic and/or a bactericidal effect or property.
  • the method for reducing the bacterial load on or in an air conditioning system can thus be a method for removing, killing or reducing the number of (living) bacteria on or in an air conditioning system.
  • the method for reducing the bacterial load on or in an air conditioning system can be a method for preventing the formation of a biofilm on or in an air conditioning system, or a method for removing a biofilm from an air conditioning system.
  • a biofilm is understood to mean, in particular, a group of microorganisms, especially bacteria, in which the cells adhere to one another on a surface.
  • the method for reducing the bacterial load on or in an air conditioning system can be a method for preventing the formation of (bacterially caused, unpleasant) odors in an air conditioning system.
  • a "bacterially caused odor” is understood to mean, in particular, an odor produced by odorants generated by bacteria.
  • an "unpleasant odor” is understood to mean, in particular, an odor that is perceived as unpleasant by at least 50% of people, and especially by at least 80% of people.
  • Sphingomonas and Methylobacteria bacteriophages have proven to be particularly suitable for preventing the formation of unpleasant odors in an air conditioning system.
  • An air conditioning system can refer in particular to the air conditioning system of a vehicle, such as a motor vehicle, especially an automobile (e.g., with a gasoline or diesel engine, including a hybrid vehicle, or an electric vehicle), a commercial vehicle (e.g., a truck or a construction vehicle), a ship, or an aircraft.
  • a vehicle such as a motor vehicle, especially an automobile (e.g., with a gasoline or diesel engine, including a hybrid vehicle, or an electric vehicle), a commercial vehicle (e.g., a truck or a construction vehicle), a ship, or an aircraft.
  • an air conditioning system can also refer to a stationary air conditioning system, such as the air conditioning system of a building.
  • the procedure involves applying bacteriophages or a component thereof to one or more parts of the air conditioning system.
  • bacteriophage refers specifically to a virus that can infect a bacterium, multiply at its expense, and ultimately lyse (dissolve) it.
  • a bacteriophage usually infects one or more specific bacteria and therefore typically exhibits a corresponding host specificity.
  • the applied component of a bacteriophage may in particular be an antibacterial component of the bacteriophage.
  • the bacteriophages, or a component thereof comprise at least one from the group consisting of Sphingomonas bacteriophages and Methylobacterium bacteriophages.
  • Sphingomonas spp. the following bacterial species have been identified in vehicle air conditioning systems: Sphingomonas spp. and Methylobacterium spp. Therefore, Sphingomonas bacteriophages and Methylobacterium bacteriophages are especially suitable for reducing the bacterial load on or in an air conditioning system.
  • the bacteriophages, or a component thereof comprise at least one from the group consisting of Bacteriophage SP-P_L_l_2022, deposited under DSM 34460, and Bacteriophage SP-P_M_l_2022, deposited under DSM 34461, or a component thereof. These bacteriophages have proven to be particularly suitable for reducing the bacterial load on or in an air conditioning system.
  • the application of bacteriophages or a component thereof comprises the application of a solution or a dispersion (in particular a suspension or an emulsion) containing the bacteriophages or a component thereof.
  • a solution or a dispersion in particular a suspension or an emulsion
  • the bacteriophages or a component thereof can be applied as a solution, for example, by dissolving the bacteriophages or a component thereof in a solvent.
  • freeze-dried bacteriophages or a component thereof were dissolved or dispersed before application. It is thus possible to freeze-dry bacteriophages or a component thereof, thereby improving their shelf life, and then dissolve or disperse them only immediately before application—for example, directly before application by the user or even by the manufacturer (so to speak, "pre-packaged"). As already mentioned, this improves the shelf life of the bacteriophages or a component thereof.
  • the solution or dispersion further contains at least one nutrient for bacteria, in particular for bacteria that may be found in air conditioning systems.
  • Bacteriophages can multiply in metabolically active bacteria and thereby kill them. Therefore, it is usually necessary to supply the bacteria with nutrients.
  • a "nutrient" is understood to mean, in particular, a substance that can serve as food for the host bacteria of the bacteriophages. Suitable nutrients include, among other things, organic substrates as well as mineral macro- and trace elements and buffer components.
  • the solution or dispersion furthermore has a Ca2+ concentration in the range of 1 to 10 mmol/l, in particular 1.5 to 5 mmol/l, more particularly 2 to 4 mmol/l, preferably about 2.5 mmol/l. It has been shown that a Ca2+- A concentration within the aforementioned range improves the antibacterial efficacy of bacteriophages or a component thereof.
  • the Ca2+ concentration can be adjusted by adding an appropriate amount of a calcium salt, such as calcium chloride, to the applied fluid.
  • the application of bacteriophages or a component thereof comprises spraying a solution or dispersion containing the bacteriophages or a component thereof onto one or more parts of the air conditioning system.
  • This can be done, for example, using a pressure cup gun, optionally with a spray lance, a metering system, or an alternative application system, as is generally known from the prior art.
  • one or more parts of the air conditioning system comprise an evaporator and/or a pollen filter housing.
  • These parts of an air conditioning system, particularly a vehicle air conditioning system, are especially susceptible to bacterial colonization and biofilm formation, making it advantageous to apply the bacteriophages, or a component thereof, directly to the target or intended site of action.
  • the present invention relates to the use of bacteriophages or a component thereof for reducing the bacterial load (for removing, killing, or reducing the number of bacteria) on or in an air conditioning system.
  • the bacteriophages or a component thereof can be used to remove, kill, or reduce the number of (live) bacteria on or in an air conditioning system, such as a vehicle air conditioning system.
  • an air conditioning system such as a vehicle air conditioning system.
  • the present invention relates to the use of bacteriophages or a component thereof to prevent the formation of a biofilm or to remove a biofilm on or in an air conditioning system.
  • the present invention relates to the use of bacteriophages or a component thereof to prevent the formation of (bacterially caused, unpleasant) odors in an air conditioning system.
  • Sphingomonas and Methylobacteria bacteriophages have proven particularly suitable for use in preventing the formation of unpleasant odors in an air conditioning system.
  • the present invention relates to a bacteriophage or a component thereof, comprising at least one from the group consisting of Bacteriophage SP-P_L_l_2022, filed under DSM 34460, and Bacteriophage SP-P_M_l_2022, filed under DSM 34461.
  • Modern car air conditioning systems typically use evaporator plates made primarily of aluminum. Manufacturers optimize the material properties through additional additives and coatings designed to extend the material's lifespan. Some of these coatings are also intended to have antimicrobial properties. In this case, they would prevent the intended investigations by inhibiting the growth of the bacteria used. Therefore, for this study, metal samples were obtained that were not coated with antimicrobial substances.
  • the sheet metal pieces were cut into equal-sized sections (e.g., 5 x 5 cm) for testing. To degrease them, the sections were washed with dish soap and then rinsed with ultrapure water until no more foam appeared. The sheet metal pieces were then pre-sterilized (dry heat at 100°C or 140°C).
  • Bacteriophages can only multiply and kill metabolically active bacteria. Therefore, it is necessary to supply the bacteria with nutrients. A high infection rate can be promoted by the presence of phages, growing bacteria, and higher concentrations of calcium carbonate (CaC). Therefore, samples with 0 mM, 2.5 mM, 5 mM, and 10 mM CaC were prepared.
  • CaC calcium carbonate
  • the experimental setup involved distributing the test bacteria (0.1 mL of a suspension with an OD550 nm of 0.5) onto metal sheets in a soft agar (1.2 mL with 4 g agar/L) containing NAR medium and 0–10 mM CaC. After cooling the agar, 250 pL of a phage solution (with approximately 10 million phage units/mL) were added dropwise. The samples were then incubated for several days and evaluated for visually detectable differences. As a control, samples were prepared using sterile SM buffer instead of phage solution. Additionally, samples were prepared where the agar film was deposited not on metal sheets but on plastic culture dishes. Comparing the data for the plastic culture dishes and the metal sheets was intended to reveal whether the metal affected the efficacy of the phages.
  • the agar was only evenly distributed on the trays after the trays had been preheated and the dosed volume doubled. Presumably, the longer exposure to higher temperatures was the cause. At 46 °C, the vitality of the bacteria was impaired. Even after 3 days of incubation, the growth remained unexpectedly low, even in cultures to which no phages were added. No visual differences were discernible between the variants on metal and plastic culture dishes.
  • the soft agar from the initial series with 10 mM CaC was transferred to a suspension medium (10 mL SM buffer) and eluted. Dilutions of the initial eluates with SM buffer were used for viable cell count determinations on NAR agar.
  • the evaluation data are summarized in Table 1.
  • the two assay variants to which phages were added showed significantly lower numbers of colony-forming units. The percentage of underreported results was 95% for the Petri dish variant and 79% for the variant with metal plates.
  • the effect of phages on bacteria colonizing the metal surface was investigated.
  • the aim was to determine whether the phages were able to reach the bacteria, a prerequisite for infection. It was important that the phage dilution was not too high to allow for successful interaction with the bacteria.
  • 25 mL of NAR broth were placed in 8 screw-top glass jars, with a metal tray placed in each jar.
  • a bacterial solution with a defined initial cell count was then prepared from a pre-culture. 1 mL of this solution was inoculated into each jar and incubated at 30 °C with gentle shaking (45 rpm) over a weekend. As expected, this resulted in turbidity and bacterial growth on the tray surfaces.
  • solutions of NAR broth and CaC with final concentrations of 0 mM, 2.5 mM, 5 mM, and 10 mM were prepared.
  • 0.1 mL of a previously diluted 1:100 phage high-titer solution was added to a 2 mL aliquot of each of the prepared solutions. This solution should then contain approximately 100 million PFU.

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Abstract

L'invention concerne un procédé de réduction de la charge bactérienne sur ou dans une installation de climatisation, le procédé comprenant l'application de bactériophages ou d'un composant de ces bactériophages sur une ou plusieurs parties de l'installation de climatisation. Cette invention concerne également l'utilisation de bactériophages ou d'un de leurs composants pour réduire les bactéries présentes sur ou dans une installation de climatisation, pour empêcher la formation d'un biofilm ou pour éliminer un biofilm sur ou dans une installation de climatisation, et/ou pour prévenir la formation d'odeurs dans une installation de climatisation. Enfin, l'invention concerne certains bactériophages en tant que tels.
PCT/EP2025/063289 2024-05-16 2025-05-14 Procédé de réduction de la charge bactérienne sur ou dans une installation de climatisation Pending WO2025238109A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102024113842.4A DE102024113842A1 (de) 2024-05-16 2024-05-16 Verfahren zur Verringerung der bakteriellen Last auf bzw. in einer Klimaanlage
DE102024113842.4 2024-05-16

Publications (2)

Publication Number Publication Date
WO2025238109A2 true WO2025238109A2 (fr) 2025-11-20
WO2025238109A3 WO2025238109A3 (fr) 2026-02-19

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DE (1) DE102024113842A1 (fr)
WO (1) WO2025238109A2 (fr)

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DE60106727T2 (de) 2000-01-11 2005-12-22 Intralytix Inc. Verfahren und vorrichtung zur reinigung mit bakteriophagen
WO2009090097A2 (fr) 2008-01-16 2009-07-23 Cedes Ag Système de sécurité pour la sécurisation d'un élément mobile guidé, en déplacement, contre des collisions involontaires
WO2010090542A2 (fr) 2009-02-06 2010-08-12 Technophage, Investigação E Desenvolvimento Em Biotecnologia, Sa Phage antibactérien, peptides phages et méthodes d'utilisation de ceux-ci
WO2019136108A1 (fr) 2018-01-02 2019-07-11 Ampliphi Biosciences Corporation Compositions de bactériophages pour le traitement d'infections à pseudomonas

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WO2010141135A2 (fr) * 2009-03-05 2010-12-09 Trustees Of Boston University Bactériophages exprimant des peptides antimicrobiennes et utilisations afférentes
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