ZA200202007B - Method for production of proteinaceous substances. - Google Patents

Method for production of proteinaceous substances. Download PDF

Info

Publication number: ZA200202007B
Authority: ZA; South Africa
Prior art keywords: vegf; plant; production; moss; culture
Prior art date: 1999-10-01

Application number

ZA200202007A

Other languages

English (en)

Inventor

Ralf Reski

Gilbert Gorr

Original Assignee

Greenovation Biotech Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-10-01

Filing date

2002-03-11

Publication date

2003-09-23

2002-03-11 Application filed by Greenovation Biotech Gmbh filed Critical Greenovation Biotech Gmbh

2003-09-23 Publication of ZA200202007B publication Critical patent/ZA200202007B/en

Links

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238000001914 filtration Methods 0.000 description 1
238000000684 flow cytometry Methods 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
230000003325 follicular Effects 0.000 description 1
230000000855 fungicidal effect Effects 0.000 description 1
238000012239 gene modification Methods 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
230000005017 genetic modification Effects 0.000 description 1
235000013617 genetically modified food Nutrition 0.000 description 1
238000000227 grinding Methods 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
238000005805 hydroxylation reaction Methods 0.000 description 1
238000005286 illumination Methods 0.000 description 1
238000012606 in vitro cell culture Methods 0.000 description 1
210000003000 inclusion body Anatomy 0.000 description 1
230000036512 infertility Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002955 isolation Methods 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
230000007774 longterm Effects 0.000 description 1
230000037353 metabolic pathway Effects 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
230000002297 mitogenic effect Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
230000000877 morphologic effect Effects 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
238000011275 oncology therapy Methods 0.000 description 1
238000005457 optimization Methods 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
229920002866 paraformaldehyde Polymers 0.000 description 1
230000002572 peristaltic effect Effects 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
230000001817 pituitary effect Effects 0.000 description 1
229930000223 plant secondary metabolite Natural products 0.000 description 1
238000004161 plant tissue culture Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000012545 processing Methods 0.000 description 1
238000012205 qualitative assay Methods 0.000 description 1
238000000275 quality assurance Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
241000894007 species Species 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
230000000707 stereoselective effect Effects 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
230000009469 supplementation Effects 0.000 description 1
230000001502 supplementing effect Effects 0.000 description 1
238000012360 testing method Methods 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
238000004448 titration Methods 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
230000014616 translation Effects 0.000 description 1
IMNIMPAHZVJRPE-UHFFFAOYSA-N triethylenediamine Chemical compound C1CN2CCN1CC2 IMNIMPAHZVJRPE-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
210000004881 tumor cell Anatomy 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
241000701447 unidentified baculovirus Species 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
230000008728 vascular permeability Effects 0.000 description 1
239000011534 wash buffer Substances 0.000 description 1
239000002699 waste material Substances 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1
239000002023 wood Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Cell Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Toxicology (AREA)
Pharmacology & Pharmacy (AREA)
Botany (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

ZA200202007A 1999-10-01 2002-03-11 Method for production of proteinaceous substances. ZA200202007B (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19947290A DE19947290A1 (de)	1999-10-01	1999-10-01	Verfahren zur Herstellung proteinöser Substanzen

Publications (1)

Publication Number	Publication Date
ZA200202007B true ZA200202007B (en)	2003-09-23

Family

ID=7924139

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ZA200202007A ZA200202007B (en)	1999-10-01	2002-03-11	Method for production of proteinaceous substances.

Country Status (31)

Country	Link
EP (1)	EP1206561B1 (fr)
JP (1)	JP2003511035A (fr)
KR (1)	KR100671217B1 (fr)
CN (1)	CN1192111C (fr)
AT (1)	ATE232904T1 (fr)
AU (1)	AU781920B2 (fr)
BG (1)	BG65487B1 (fr)
BR (1)	BR0014671A (fr)
CA (1)	CA2381995C (fr)
CZ (1)	CZ293818B6 (fr)
DE (2)	DE19947290A1 (fr)
DK (1)	DK1206561T3 (fr)
EE (1)	EE05093B1 (fr)
ES (1)	ES2192545T3 (fr)
HK (1)	HK1054053B (fr)
HR (1)	HRP20020250B1 (fr)
HU (1)	HU228206B1 (fr)
IL (2)	IL148638A0 (fr)
IS (1)	IS6299A (fr)
MX (1)	MXPA02003359A (fr)
NO (1)	NO329774B1 (fr)
NZ (1)	NZ517996A (fr)
PL (1)	PL200252B1 (fr)
PT (1)	PT1206561E (fr)
RS (1)	RS50075B (fr)
RU (1)	RU2250264C2 (fr)
SK (1)	SK287740B6 (fr)
TR (1)	TR200200700T2 (fr)
UA (1)	UA72552C2 (fr)
WO (1)	WO2001025456A2 (fr)
ZA (1)	ZA200202007B (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE60333201D1 (de) *	2002-09-12	2010-08-12	Greenovation Biotech Gmbh	Verfahren zur herstellung von proteinen
EP1398383A1 (fr) *	2002-09-12	2004-03-17	greenovation Biotech GmbH	Procédé de production de protéines
US7781197B2 (en)	2002-12-20	2010-08-24	Greenovation Biotech Gmbh	Transformed bryophyte cell having disrupted endogenous alpha 1,3-fucosyl and beta 1,2-xylosyl transferase encoding nucleotide sequences for the production of heterologous glycosylated proteins
DK1431394T3 (da) *	2002-12-20	2006-12-11	Greenovation Biotech Gmbh	Fremgangsmåde til fremstilling af heterologe, glycosylerede proteiner i mosplanteceller
EP1502954A1 (fr)	2003-07-31	2005-02-02	greenovation Biotech GmbH	Utilisation d'un construct comprenant de séquences de récombinaison pour expression génétique ameliorée dans mousse.
DE60326716D1 (de) *	2003-08-11	2009-04-30	Greenovation Biotech Gmbh	Promoter Sequenzen aus Moos und dessen Verwendung
DE602006015195D1 (de)	2005-07-12	2010-08-12	Greenovation Biotech Gmbh	Verbesserungen der proteinproduktion oder in zusammenhang damit
EP1905825A1 (fr)	2006-09-29	2008-04-02	greenovation Biotech GmbH	Galactosyltransférase
KR200451866Y1 (ko) *	2008-07-28	2011-01-14	구동석	휴대폰 및 귀중품 보관함
RU2415936C1 (ru) *	2010-01-11	2011-04-10	Учреждение Российской академии наук Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН)	Способ секреции зеленого флуоресцентного белка (gfp) из растительных клеток
JP5641232B2 (ja) *	2010-11-24	2014-12-17	石川県公立大学法人	オゴノリ由来のシクロオキシゲナーゼの遺伝子及び該遺伝子を利用するプロスタグランジン類生産方法
EP2653548A1 (fr)	2012-04-17	2013-10-23	Greenovation Biotech GmbH	Procédé d'augmentation de la sécrétion d'une protéine recombinante
RU2663131C1 (ru) *	2017-09-06	2018-08-01	федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ)	Носитель для культивирования клеток человека и животных
EP3808854A1 (fr) *	2019-10-17	2021-04-21	eleva GmbH	Procédé d'obtention de matériau des surfaces de cellules végétales
CN115806930A (zh) *	2022-12-20	2023-03-17	河北师范大学	小反扭藓配子体的培养基及培养方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NL8901932A (nl) *	1989-07-26	1991-02-18	Mogen Int	Produktie van heterologe eiwitten in planten of plantecellen.
AU6504796A (en) *	1995-07-20	1997-02-18	Washington State University Research Foundation	Production of secreted foreign polypeptides in plant cell culture
DE19629402C2 (de) *	1996-07-20	1998-05-14	Dirk Dr Voeste	Verwendung von transformierten vaskulären Wasserpflanzen zur Produktion von arteigenen oder artfremden Substanzen
WO1998021348A1 (fr) *	1996-11-12	1998-05-22	Battelle Memorial Institute	Procede de production facteurs de croissance humain a partir de plantes entieres ou de cultures de cellules vegetales
WO1998036085A1 (fr) *	1997-02-13	1998-08-20	Applied Phytologics, Inc.	Production de proteines matures dans des plantes
US6096546A (en) *	1998-01-30	2000-08-01	Board Of Trustees, Rutgers, The State University Of New Jersey	Methods for recovering polypeptides from plants and portions thereof

1999
- 1999-10-01 DE DE19947290A patent/DE19947290A1/de not_active Withdrawn
2000
- 2000-09-27 PL PL354716A patent/PL200252B1/pl unknown
- 2000-09-27 HK HK03106378.6A patent/HK1054053B/zh not_active IP Right Cessation
- 2000-09-27 CZ CZ20021061A patent/CZ293818B6/cs not_active IP Right Cessation
- 2000-09-27 HU HU0202628A patent/HU228206B1/hu unknown
- 2000-09-27 DE DE50001299T patent/DE50001299D1/de not_active Expired - Lifetime
- 2000-09-27 BR BR0014671-4A patent/BR0014671A/pt not_active Application Discontinuation
- 2000-09-27 ES ES00972602T patent/ES2192545T3/es not_active Expired - Lifetime
- 2000-09-27 PT PT00972602T patent/PT1206561E/pt unknown
- 2000-09-27 CA CA002381995A patent/CA2381995C/fr not_active Expired - Lifetime
- 2000-09-27 NZ NZ517996A patent/NZ517996A/xx not_active IP Right Cessation
- 2000-09-27 WO PCT/DE2000/003374 patent/WO2001025456A2/fr not_active Ceased
- 2000-09-27 KR KR1020027004246A patent/KR100671217B1/ko not_active Expired - Lifetime
- 2000-09-27 UA UA2002043701A patent/UA72552C2/uk unknown
- 2000-09-27 AU AU11295/01A patent/AU781920B2/en not_active Expired
- 2000-09-27 CN CNB008164967A patent/CN1192111C/zh not_active Expired - Lifetime
- 2000-09-27 AT AT00972602T patent/ATE232904T1/de active
- 2000-09-27 DK DK00972602T patent/DK1206561T3/da active
- 2000-09-27 RS YUP-246/02A patent/RS50075B/sr unknown
- 2000-09-27 MX MXPA02003359A patent/MXPA02003359A/es active IP Right Grant
- 2000-09-27 IL IL14863800A patent/IL148638A0/xx unknown
- 2000-09-27 TR TR2002/00700T patent/TR200200700T2/xx unknown
- 2000-09-27 SK SK415-2002A patent/SK287740B6/sk not_active IP Right Cessation
- 2000-09-27 HR HR20020250 patent/HRP20020250B1/xx not_active IP Right Cessation
- 2000-09-27 RU RU2002111666/13A patent/RU2250264C2/ru not_active IP Right Cessation
- 2000-09-27 EE EEP200200159A patent/EE05093B1/xx not_active IP Right Cessation
- 2000-09-27 JP JP2001528608A patent/JP2003511035A/ja active Pending
- 2000-09-27 EP EP00972602A patent/EP1206561B1/fr not_active Expired - Lifetime
2002
- 2002-03-11 ZA ZA200202007A patent/ZA200202007B/en unknown
- 2002-03-12 IL IL148638A patent/IL148638A/en active IP Right Grant
- 2002-03-12 NO NO20021201A patent/NO329774B1/no not_active IP Right Cessation
- 2002-03-13 IS IS6299A patent/IS6299A/is unknown
- 2002-03-22 BG BG106547A patent/BG65487B1/bg unknown

Also Published As

Publication number	Publication date
BG106547A (en)	2003-04-30
CN1192111C (zh)	2005-03-09
NZ517996A (en)	2003-06-30
IS6299A (is)	2002-03-13
EE200200159A (et)	2003-04-15
PL354716A1 (en)	2004-02-09
BG65487B1 (bg)	2008-09-30
HK1054053B (zh)	2005-10-28
RU2002111666A (ru)	2004-03-10
NO329774B1 (no)	2010-12-13
EE05093B1 (et)	2008-10-15
AU781920B2 (en)	2005-06-23
HU228206B1 (en)	2013-01-28
BR0014671A (pt)	2002-07-23
DE19947290A1 (de)	2001-04-19
ES2192545T3 (es)	2003-10-16
KR100671217B1 (ko)	2007-01-18
MXPA02003359A (es)	2002-10-04
DE50001299D1 (de)	2003-03-27
EP1206561A2 (fr)	2002-05-22
NO20021201L (no)	2002-05-08
RU2250264C2 (ru)	2005-04-20
IL148638A (en)	2008-12-29
IL148638A0 (en)	2002-09-12
ATE232904T1 (de)	2003-03-15
CA2381995C (fr)	2010-01-05
CZ293818B6 (cs)	2004-08-18
KR20020065478A (ko)	2002-08-13
SK287740B6 (sk)	2011-08-04
SK4152002A3 (en)	2003-01-09
HUP0202628A2 (hu)	2002-12-28
HRP20020250A2 (en)	2004-02-29
YU24602A (sh)	2005-07-19
RS50075B (sr)	2009-01-22
JP2003511035A (ja)	2003-03-25
HK1054053A1 (en)	2003-11-14
PT1206561E (pt)	2003-06-30
WO2001025456A3 (fr)	2001-12-27
DK1206561T3 (da)	2003-05-26
CA2381995A1 (fr)	2001-04-12
AU1129501A (en)	2001-05-10
CZ20021061A3 (cs)	2002-07-17
PL200252B1 (pl)	2008-12-31
WO2001025456A2 (fr)	2001-04-12
HUP0202628A3 (en)	2004-10-28
TR200200700T2 (tr)	2003-01-21
UA72552C2 (en)	2005-03-15
HRP20020250B1 (en)	2004-12-31
NO20021201D0 (no)	2002-03-12
CN1402791A (zh)	2003-03-12
EP1206561B1 (fr)	2003-02-19

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US6740526B1 (en)	2004-05-25	Quantitative transient protein expression in plant tissue culture
US20030084484A1 (en)	2003-05-01	Commercial use of arabidopsis for production of human and animal therapeutic and diagnostic proteins
Shvedova et al.	2023	Optimization of factors affecting the efficiency of Agrobacterium-mediated transformation of Wolffia arrhiza
WO2001020974A1 (fr)	2001-03-29	Expression quantitative de proteines transitoire dans une culture de tissu vegetal
Liu	2001	Expression of Green Fluorescent Protein in Transgenic Tobacco Cell Suspension Culture
Guan	2003	Fluorescent protein reporter for monitoring transgenic plant cell cultures
JP2005102652A (ja)	2005-04-21	形質転換細胞、および該細胞を用いたタンパク質の生産方法、並びにタンパク質生産キット
AU2002355830A1 (en)	2003-02-17	Commercial use of Arabidopsis for production of human and animal therapeutic and diagnostic proteins
KR20050027836A (ko)	2005-03-21	식물체에서 발현된 염기성 섬유아세포 성장인자
AU2002312362A1 (en)	2002-12-23	Production of proteins in plants
WO2005077153A1 (fr)	2005-08-25	Bioreacteur utilisant des plantes vivipares
KR20050027839A (ko)	2005-03-21	식물체에서 발현된 인간 과립구 대식세포 집락 촉진인자