ZA91500B - Process for the production of thrombin and high purity thrombin preparations thereby obtained - Google Patents

Process for the production of thrombin and high purity thrombin preparations thereby obtained

Info

Publication number: ZA91500B
Authority: ZA; South Africa
Prior art keywords: thrombin; production; preparations; high purity; column
Prior art date: 1990-01-24

Application number

ZA91500A

Other languages

English (en)

Inventor

Carmel Crowley

Crowley Carmel

Timothy Gerard Sheehan

Gerard Sheehan Timothy

Frederick Gerard Ward

Gerard Ward Frederick

Original Assignee

Warner Lambert Pottery Road Li

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-01-24

Filing date

1991-01-23

Publication date

1991-11-27

1991-01-23 Application filed by Warner Lambert Pottery Road Li filed Critical Warner Lambert Pottery Road Li

1991-11-27 Publication of ZA91500B publication Critical patent/ZA91500B/xx

Links

108090000190 Thrombin Proteins 0.000 title abstract 6
229960004072 thrombin Drugs 0.000 title abstract 6
238000002360 preparation method Methods 0.000 title abstract 3
238000004519 manufacturing process Methods 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
229920000936 Agarose Polymers 0.000 abstract 2
239000007788 liquid Substances 0.000 abstract 2
PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical compound CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 abstract 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
108010094028 Prothrombin Proteins 0.000 abstract 1
102100027378 Prothrombin Human genes 0.000 abstract 1
108010000499 Thromboplastin Proteins 0.000 abstract 1
102000002262 Thromboplastin Human genes 0.000 abstract 1
238000005349 anion exchange Methods 0.000 abstract 1
229910052791 calcium Inorganic materials 0.000 abstract 1
239000011575 calcium Substances 0.000 abstract 1
238000005341 cation exchange Methods 0.000 abstract 1
238000007865 diluting Methods 0.000 abstract 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 abstract 1
239000000706 filtrate Substances 0.000 abstract 1
238000001914 filtration Methods 0.000 abstract 1
230000023597 hemostasis Effects 0.000 abstract 1
239000008363 phosphate buffer Substances 0.000 abstract 1
239000002953 phosphate buffered saline Substances 0.000 abstract 1
229940039716 prothrombin Drugs 0.000 abstract 1
239000000725 suspension Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6429—Thrombin (3.4.21.5)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21005—Thrombin (3.4.21.5)

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Public Health (AREA)
Pharmacology & Pharmacy (AREA)
Hematology (AREA)
Animal Behavior & Ethology (AREA)
Diabetes (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Enzymes And Modification Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

ZA91500A 1990-01-24 1991-01-23 Process for the production of thrombin and high purity thrombin preparations thereby obtained ZA91500B (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IE26690A IE73210B1 (en)	1990-01-24	1990-01-24	Process for the production of thrombin and high purity thrombin preparation thereby obtained

Publications (1)

Publication Number	Publication Date
ZA91500B true ZA91500B (en)	1991-11-27

Family

ID=11009586

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ZA91500A ZA91500B (en)	1990-01-24	1991-01-23	Process for the production of thrombin and high purity thrombin preparations thereby obtained

Country Status (8)

Country	Link
US (1)	US5151355A (de)
EP (1)	EP0439156B1 (de)
JP (1)	JP3276964B2 (de)
AT (1)	ATE140026T1 (de)
CA (1)	CA2034826A1 (de)
DE (1)	DE69120550D1 (de)
IE (1)	IE73210B1 (de)
ZA (1)	ZA91500B (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5281528A (en) *	1989-12-18	1994-01-25	Warner-Lambert Company	Process for purified thromboplastin for ultra-pure thrombin preparation
AU642987B2 (en) *	1991-03-25	1993-11-04	Warner-Lambert Company	Ultra-pure thrombin preparation
ATE143411T1 (de) *	1991-03-28	1996-10-15	Warner Lambert Co	Hochreine thrombinzubereitung
AT402891B (de) *	1991-06-20	1997-09-25	Immuno Ag	Verfahren zur herstellung eines inaktivierten blutproduktes
FR2679251B1 (fr) *	1991-07-18	1993-11-12	Nord Assoc Essor Transfusion San	Procede de preparation d'un concentre de thrombine humaine destine a un usage therapeutique.
AT398079B (de) *	1991-11-04	1994-09-26	Immuno Ag	Präparation mit thrombinaktivität sowie verfahren zu ihrer herstellung
DE4137996A1 (de) *	1991-11-19	1993-05-27	Behringwerke Ag	Verfahren zur herstellung eines virussicheren thrombinkonzentrates
AT397390B (de) *	1992-04-06	1994-03-25	Immuno Ag	Verfahren zur spaltung von proteinen
US5792623A (en) *	1992-04-06	1998-08-11	Immuno Aktiengesellschaft	Method for producing activated blood factors with a protease and a detergent
AT399818B (de) *	1992-04-24	1995-07-25	Immuno Ag	Verfahren zur herstellung einer hochgereinigten virussicheren faktor viii-präparation
DK83092D0 (da) *	1992-06-24	1992-06-24	Unes As	Fremgangsmaade til udvinding af thrombin
WO1994013329A1 (de) *	1992-12-16	1994-06-23	Immuno Aktiengesellschaft	Verfahren zur herstellung eines virussicheren biologischen präparates
DE4320294A1 (de) *	1993-06-18	1994-12-22	Immuno Ag	Verwendung von humanem Protein C zur Verhinderung und Behandlung von Thrombozytenablagerungen
US5795780A (en) *	1993-06-23	1998-08-18	Bristol-Myers Squibb Company	Method of use of autologous thrombin blood fraction in a cell culture with keratinocytes
JPH07308190A (ja) *	1994-05-18	1995-11-28	Green Cross Corp:The	トロンビンの製造方法
US5506127A (en) *	1994-09-21	1996-04-09	Proba; Zbigniew	Therapeutic grade thrombin produced by chromatography
US5677162A (en) *	1995-05-01	1997-10-14	New York Blood Center, Inc.	Method for activating prothrombin to thrombin
WO2000062828A1 (en)	1996-04-30	2000-10-26	Medtronic, Inc.	Autologous fibrin sealant and method for making the same
US6168788B1 (en)	1997-09-26	2001-01-02	Leon Wortham	Fibrin glue without fibrinogen and biosealant compositions and methods
US6274090B1 (en)	1998-08-05	2001-08-14	Thermogenesis Corp.	Apparatus and method of preparation of stable, long term thrombin from plasma and thrombin formed thereby
US6472162B1 (en)	1999-06-04	2002-10-29	Thermogenesis Corp.	Method for preparing thrombin for use in a biological glue
DE10012732A1 (de) *	2000-03-18	2001-09-20	Aventis Behring Gmbh	Thrombin-Zubereitungen und Verfahren zu ihrer Herstellung
GB0216002D0 (en) *	2002-07-10	2002-08-21	Nat Blood Authority	Process and composition
US20040243044A1 (en) *	2003-06-02	2004-12-02	Penegor Stephen A.	Hemostatic wound dressing
US20060270014A1 (en) *	2005-05-26	2006-11-30	Dan Pawlak	Thrombin purification
US20060270015A1 (en) *	2005-05-26	2006-11-30	Dan Pawlak	Thrombin purification
US9932388B2 (en)	2014-11-13	2018-04-03	Hemarus Therapeutics Limited	Chromatographic process for producing high purity fibrinogen and thrombin
CN107406840B (zh) *	2015-02-25	2021-05-28	奥姆里克斯生物药品有限公司	用于纯化和定量凝血酶及其降解多肽的方法
ES2791982T3 (es) *	2015-02-25	2020-11-06	Omrix Biopharmaceuticals Ltd	Método para purificar y cuantificar la trombina y sus polipéptidos de degradación
CN113438928B (zh)	2019-03-06	2024-10-29	甘布罗伦迪亚股份公司	用于递送凝血酶激活的纤维蛋白密封剂的装置和方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE265329C (de) *
US4286056A (en) *	1980-01-28	1981-08-25	Baxter Travenol Laboratories, Inc.	Method for making therapeutic enzyme compositions
US4363319A (en) *	1980-06-30	1982-12-14	Applied Medical Devices, Inc.	Ready-to-use bandage incorporating a coagulant composition and method of preparing same
US4380511A (en) *	1981-08-12	1983-04-19	Miles Laboratories, Inc.	Purification of bovine thrombin
US4473553A (en) *	1983-12-02	1984-09-25	Miles Laboratories, Inc.	Process for producing a lipoprotein-poor concentrate of coagulation factors VII and VIIa
US4470969A (en) *	1983-12-02	1984-09-11	Miles Laboratories, Inc.	Process for producing a concentrate of coagulation factors VII and VIIa
KR930007434B1 (ko) *	1984-09-21	1993-08-10	베링거 잉겔하임 인터내셔날 게엠베하	혈액응고 억제 단백질 제조방법
US4637932A (en) *	1984-10-15	1987-01-20	Miles Laboratories, Inc.	Process for producing a concentrate enriched in coagulation factors VII and VIIa
US4696812A (en) *	1985-10-28	1987-09-29	Warner-Lambert Company	Thrombin preparations
US4965203A (en) *	1987-01-28	1990-10-23	Warner-Lambert Company	Purified thrombin preparations
ZA88227B (en) *	1987-01-28	1988-06-30	Warner-Lambert Company	Thrombin preparations
DE3877529T2 (de) *	1987-10-23	1996-11-07	Centre Regional De Transfusion	Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat und dessen therapeutische Verwendung.
SU1527261A1 (ru) *	1988-03-21	1989-12-07	Институт Химической И Биологической Физики Ан Эсср	Способ получени тромбина

1990
- 1990-01-24 IE IE26690A patent/IE73210B1/en not_active IP Right Cessation
1991
- 1991-01-23 JP JP02134891A patent/JP3276964B2/ja not_active Expired - Fee Related
- 1991-01-23 EP EP91100853A patent/EP0439156B1/de not_active Expired - Lifetime
- 1991-01-23 CA CA002034826A patent/CA2034826A1/en not_active Abandoned
- 1991-01-23 US US07/644,800 patent/US5151355A/en not_active Expired - Lifetime
- 1991-01-23 DE DE69120550T patent/DE69120550D1/de not_active Expired - Lifetime
- 1991-01-23 ZA ZA91500A patent/ZA91500B/xx unknown
- 1991-01-23 AT AT91100853T patent/ATE140026T1/de not_active IP Right Cessation

Also Published As

Publication number	Publication date
EP0439156A1 (de)	1991-07-31
ATE140026T1 (de)	1996-07-15
US5151355A (en)	1992-09-29
JP3276964B2 (ja)	2002-04-22
JPH04211372A (ja)	1992-08-03
DE69120550D1 (de)	1996-08-08
CA2034826A1 (en)	1991-07-25
EP0439156B1 (de)	1996-07-03
IE900266A1 (en)	1991-07-31
IE73210B1 (en)	1997-05-07

Publication	Publication Date	Title
ZA91500B (en)	1991-11-27	Process for the production of thrombin and high purity thrombin preparations thereby obtained
EP0056629B1 (de)	1985-05-15	Verfahren zur Reinigung der Blutgerinnungsfaktoren II, VII, IX und/oder X und danach hergestellte Präparationen
Chibber et al.	1974	[48] Plasminogen
ATE94206T1 (de)	1993-09-15	Enzymhaltige zubereitung und eine solche zubereitung enthaltendes detergens.
EP0391526A3 (de)	1991-01-09	Reinigungsverfahren für Antikörper
CA2160531A1 (en)	1994-10-27	Process for purifying collagenase
ATE70301T1 (de)	1991-12-15	Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
KR970009949B1 (en)	1997-06-19	Novel serine proteolytic enzyme and its process
US4137127A (en)	1979-01-30	Process for the preparation of thrombin-like enzymes from snake venoms
AU4815885A (en)	1986-04-10	Enzymatic process
Chavin et al.	1984	Blood clotting factor IX. Loss of activity after cleavage of sialic acid residues.
EP0092845A2 (de)	1983-11-02	Verfahren zur Enzymreinigung
AU8003294A (en)	1995-05-22	Human activated protein c preparation and process for producing the same
US6200791B1 (en)	2001-03-13	Method of purifying thrombin-like protease enzymes obtained from snake venom
GR3006419T3 (de)	1993-06-21
Williams et al.	1989	Purification and properties of a procoagulant from peninsula tiger snake (Notechis ater niger) venom
Teng et al.	1981	Production of Factor X and Factor Xa variants with thrombin, acutin and by autolysis
GB1293795A (en)	1972-10-25	Improvements relating to anticoagulants
AU1239795A (en)	1995-07-13	Purified stable liquid prealbumin
NO931773L (no)	1993-11-16	Fremgangsm}te ved fremstilling av fibrinogen
GB1488463A (en)	1977-10-12	Meningococcal polysaccharide vaccine
US4957864A (en)	1990-09-18	Novel plasminogen activator and its preparation process
NO174111C (no)	1994-03-16	Fremgangsmåte for rensing av vevsplasminogenaktivatorer (t-PA)
EP0151996A3 (en)	1986-10-01	Novel purified plasminogen activators, process for their production and thrombolytic composition containing them
Workman Jr et al.	1978	On the preparation of bovine α-thrombin