ATE140026T1 - Verfahren zur herstellung von thrombin und danach erhaltene hochgereinigte thrombinzusammensetzung - Google Patents

Verfahren zur herstellung von thrombin und danach erhaltene hochgereinigte thrombinzusammensetzung

Info

Publication number: ATE140026T1
Authority: AT; Austria
Prior art keywords: thrombin; composition obtained; highly purified; obtained therefrom; producing
Prior art date: 1990-01-24

Application number

AT91100853T

Other languages

English (en)

Inventor

Carmel Crowley

Frederick Gerard Ward

Timothy Gerard Sheehan

Original Assignee

Warner Lambert Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-01-24

Filing date

1991-01-23

Publication date

1996-07-15

1991-01-23 Application filed by Warner Lambert Co filed Critical Warner Lambert Co

1996-07-15 Application granted granted Critical

1996-07-15 Publication of ATE140026T1 publication Critical patent/ATE140026T1/de

Links

108090000190 Thrombin Proteins 0.000 title abstract 6
229960004072 thrombin Drugs 0.000 title abstract 6
238000004519 manufacturing process Methods 0.000 title abstract 2
229920000936 Agarose Polymers 0.000 abstract 2
239000007788 liquid Substances 0.000 abstract 2
238000002360 preparation method Methods 0.000 abstract 2
PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical compound CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 abstract 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
108010094028 Prothrombin Proteins 0.000 abstract 1
102100027378 Prothrombin Human genes 0.000 abstract 1
108010000499 Thromboplastin Proteins 0.000 abstract 1
102000002262 Thromboplastin Human genes 0.000 abstract 1
238000005349 anion exchange Methods 0.000 abstract 1
229910052791 calcium Inorganic materials 0.000 abstract 1
239000011575 calcium Substances 0.000 abstract 1
238000005341 cation exchange Methods 0.000 abstract 1
238000007865 diluting Methods 0.000 abstract 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 abstract 1
239000000706 filtrate Substances 0.000 abstract 1
238000001914 filtration Methods 0.000 abstract 1
230000023597 hemostasis Effects 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
239000008363 phosphate buffer Substances 0.000 abstract 1
239000002953 phosphate buffered saline Substances 0.000 abstract 1
229940039716 prothrombin Drugs 0.000 abstract 1
239000000725 suspension Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6429—Thrombin (3.4.21.5)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21005—Thrombin (3.4.21.5)

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Hematology (AREA)
Diabetes (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Enzymes And Modification Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

AT91100853T 1990-01-24 1991-01-23 Verfahren zur herstellung von thrombin und danach erhaltene hochgereinigte thrombinzusammensetzung ATE140026T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IE26690A IE73210B1 (en)	1990-01-24	1990-01-24	Process for the production of thrombin and high purity thrombin preparation thereby obtained

Publications (1)

Publication Number	Publication Date
ATE140026T1 true ATE140026T1 (de)	1996-07-15

Family

ID=11009586

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT91100853T ATE140026T1 (de)	1990-01-24	1991-01-23	Verfahren zur herstellung von thrombin und danach erhaltene hochgereinigte thrombinzusammensetzung

Country Status (8)

Country	Link
US (1)	US5151355A (de)
EP (1)	EP0439156B1 (de)
JP (1)	JP3276964B2 (de)
AT (1)	ATE140026T1 (de)
CA (1)	CA2034826A1 (de)
DE (1)	DE69120550D1 (de)
IE (1)	IE73210B1 (de)
ZA (1)	ZA91500B (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5281528A (en) *	1989-12-18	1994-01-25	Warner-Lambert Company	Process for purified thromboplastin for ultra-pure thrombin preparation
AU642987B2 (en) *	1991-03-25	1993-11-04	Warner-Lambert Company	Ultra-pure thrombin preparation
ATE143411T1 (de) *	1991-03-28	1996-10-15	Warner Lambert Co	Hochreine thrombinzubereitung
AT402891B (de) *	1991-06-20	1997-09-25	Immuno Ag	Verfahren zur herstellung eines inaktivierten blutproduktes
FR2679251B1 (fr) *	1991-07-18	1993-11-12	Nord Assoc Essor Transfusion San	Procede de preparation d'un concentre de thrombine humaine destine a un usage therapeutique.
AT398079B (de) *	1991-11-04	1994-09-26	Immuno Ag	Präparation mit thrombinaktivität sowie verfahren zu ihrer herstellung
DE4137996A1 (de) *	1991-11-19	1993-05-27	Behringwerke Ag	Verfahren zur herstellung eines virussicheren thrombinkonzentrates
US5792623A (en) *	1992-04-06	1998-08-11	Immuno Aktiengesellschaft	Method for producing activated blood factors with a protease and a detergent
AT397390B (de) *	1992-04-06	1994-03-25	Immuno Ag	Verfahren zur spaltung von proteinen
AT399818B (de) *	1992-04-24	1995-07-25	Immuno Ag	Verfahren zur herstellung einer hochgereinigten virussicheren faktor viii-präparation
DK83092D0 (da) *	1992-06-24	1992-06-24	Unes As	Fremgangsmaade til udvinding af thrombin
AU6653094A (en) *	1992-12-16	1994-07-04	Immuno Aktiengesellschaft	Process for preparing a virus-safe biological composition
DE4320294A1 (de) *	1993-06-18	1994-12-22	Immuno Ag	Verwendung von humanem Protein C zur Verhinderung und Behandlung von Thrombozytenablagerungen
US5795780A (en) *	1993-06-23	1998-08-18	Bristol-Myers Squibb Company	Method of use of autologous thrombin blood fraction in a cell culture with keratinocytes
JPH07308190A (ja) *	1994-05-18	1995-11-28	Green Cross Corp:The	トロンビンの製造方法
US5506127A (en) *	1994-09-21	1996-04-09	Proba; Zbigniew	Therapeutic grade thrombin produced by chromatography
US5677162A (en) *	1995-05-01	1997-10-14	New York Blood Center, Inc.	Method for activating prothrombin to thrombin
WO2000062828A1 (en)	1996-04-30	2000-10-26	Medtronic, Inc.	Autologous fibrin sealant and method for making the same
US6168788B1 (en)	1997-09-26	2001-01-02	Leon Wortham	Fibrin glue without fibrinogen and biosealant compositions and methods
US6274090B1 (en)	1998-08-05	2001-08-14	Thermogenesis Corp.	Apparatus and method of preparation of stable, long term thrombin from plasma and thrombin formed thereby
US6472162B1 (en)	1999-06-04	2002-10-29	Thermogenesis Corp.	Method for preparing thrombin for use in a biological glue
DE10012732A1 (de)	2000-03-18	2001-09-20	Aventis Behring Gmbh	Thrombin-Zubereitungen und Verfahren zu ihrer Herstellung
GB0216002D0 (en) *	2002-07-10	2002-08-21	Nat Blood Authority	Process and composition
US20040243044A1 (en) *	2003-06-02	2004-12-02	Penegor Stephen A.	Hemostatic wound dressing
US20060270015A1 (en) *	2005-05-26	2006-11-30	Dan Pawlak	Thrombin purification
US20060270014A1 (en) *	2005-05-26	2006-11-30	Dan Pawlak	Thrombin purification
US9932388B2 (en)	2014-11-13	2018-04-03	Hemarus Therapeutics Limited	Chromatographic process for producing high purity fibrinogen and thrombin
US10066224B2 (en) *	2015-02-25	2018-09-04	Omrix Biopharmaceuticals Ltd.	Method for purifying and quantifying thrombin and its degradation polypeptides
ES2791982T3 (es) *	2015-02-25	2020-11-06	Omrix Biopharmaceuticals Ltd	Método para purificar y cuantificar la trombina y sus polipéptidos de degradación
EP3934542B1 (de)	2019-03-06	2025-04-23	Gambro Lundia AB	Vorrichtung und verfahren zur abgabe eines thrombinaktivierten fibrinversiegelers

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE265329C (de) *
US4286056A (en) *	1980-01-28	1981-08-25	Baxter Travenol Laboratories, Inc.	Method for making therapeutic enzyme compositions
US4363319A (en) *	1980-06-30	1982-12-14	Applied Medical Devices, Inc.	Ready-to-use bandage incorporating a coagulant composition and method of preparing same
US4380511A (en) *	1981-08-12	1983-04-19	Miles Laboratories, Inc.	Purification of bovine thrombin
US4473553A (en) *	1983-12-02	1984-09-25	Miles Laboratories, Inc.	Process for producing a lipoprotein-poor concentrate of coagulation factors VII and VIIa
US4470969A (en) *	1983-12-02	1984-09-11	Miles Laboratories, Inc.	Process for producing a concentrate of coagulation factors VII and VIIa
DK166587B1 (da) *	1984-09-21	1993-06-14	Boehringer Ingelheim Int	Blodkoagulationshaemmende proteiner, fremgangsmaade til deres fremstilling samt deres anvendelse
US4637932A (en) *	1984-10-15	1987-01-20	Miles Laboratories, Inc.	Process for producing a concentrate enriched in coagulation factors VII and VIIa
US4696812A (en) *	1985-10-28	1987-09-29	Warner-Lambert Company	Thrombin preparations
US4965203A (en) *	1987-01-28	1990-10-23	Warner-Lambert Company	Purified thrombin preparations
ZA88227B (en) *	1987-01-28	1988-06-30	Warner-Lambert Company	Thrombin preparations
ES2045167T5 (es) *	1987-10-23	1996-07-01	Centre Regional De Transfusion	Preparacion de concentrado de factor ix humano de elevada pureza y de otras proteinas plasmaticas.
SU1527261A1 (ru) *	1988-03-21	1989-12-07	Институт Химической И Биологической Физики Ан Эсср	Способ получени тромбина

1990
- 1990-01-24 IE IE26690A patent/IE73210B1/en not_active IP Right Cessation
1991
- 1991-01-23 JP JP02134891A patent/JP3276964B2/ja not_active Expired - Fee Related
- 1991-01-23 DE DE69120550T patent/DE69120550D1/de not_active Expired - Lifetime
- 1991-01-23 EP EP91100853A patent/EP0439156B1/de not_active Expired - Lifetime
- 1991-01-23 CA CA002034826A patent/CA2034826A1/en not_active Abandoned
- 1991-01-23 AT AT91100853T patent/ATE140026T1/de not_active IP Right Cessation
- 1991-01-23 ZA ZA91500A patent/ZA91500B/xx unknown
- 1991-01-23 US US07/644,800 patent/US5151355A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
JPH04211372A (ja)	1992-08-03
IE73210B1 (en)	1997-05-07
EP0439156A1 (de)	1991-07-31
CA2034826A1 (en)	1991-07-25
US5151355A (en)	1992-09-29
JP3276964B2 (ja)	2002-04-22
EP0439156B1 (de)	1996-07-03
DE69120550D1 (de)	1996-08-08
IE900266A1 (en)	1991-07-31
ZA91500B (en)	1991-11-27

Legal Events

Date	Code	Title	Description
1997-01-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE140026T1 (de)	1996-07-15	Verfahren zur herstellung von thrombin und danach erhaltene hochgereinigte thrombinzusammensetzung
DE68915675T3 (de)	2002-08-14	Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.
EP0056629B1 (de)	1985-05-15	Verfahren zur Reinigung der Blutgerinnungsfaktoren II, VII, IX und/oder X und danach hergestellte Präparationen
EP0391526A3 (de)	1991-01-09	Reinigungsverfahren für Antikörper
ATE70301T1 (de)	1991-12-15	Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
ATE42178T1 (de)	1989-05-15	Zusammengesetztes fleischprodukt und verfahren zu dessen herstellung.
DE69330807D1 (de)	2001-10-31	Verfahren zur Herstellung von gereinigtem Enzym
DE3767311D1 (de)	1991-02-21	Thrombin bindender stoff und verfahren zu seiner herstellung.
US4137127A (en)	1979-01-30	Process for the preparation of thrombin-like enzymes from snake venoms
ATE56755T1 (de)	1990-10-15	Stamm von clostridium acetobutylleum und verfahren zur herstellung desselben.
ATE121418T1 (de)	1995-05-15	Verfahren zur proteinreinigung.
ATE119196T1 (de)	1995-03-15	Verfahren zur herstellung einer an faktor-viia angereicherten fraktion und ihre verwendung als arzneimittel.
ATE84971T1 (de)	1993-02-15	Verfahren zur beseitigung von pertussisendotoxin, ein pertussistoxoid und seine herstellung.
ATE95836T1 (de)	1993-10-15	Zum abbau von glycosaminoglycan faehige enzymzusammensetzung und verfahren zur herstellung dieser zusammensetzung.
ATE270327T1 (de)	2004-07-15	Verfahren zur präparation von humanem aktiviertem protein c
EP0883684B1 (de)	2004-11-24	Verfahren zur reinigung von thrombinähnlichen proteasen aus schlangengiften
DE68919282D1 (de)	1994-12-15	Verfahren zur Herstellung von Cephalosporin und Zwischenverbindungen.
Williams et al.	1989	Purification and properties of a procoagulant from peninsula tiger snake (Notechis ater niger) venom
EP0776969B1 (de)	2004-09-22	Verfahren zur Herstellung von Proteinen durch kontrollierte proteolytische spaltung von Pro-Proteinen
US3711376A (en)	1973-01-16	Coagulants
DE3585416D1 (de)	1992-04-02	Verfahren zur herstellung eines biologischen stoffes.
DE59308942D1 (de)	1998-10-08	Verfahren zur Bestimmung von Fibrinogen
DE68928634D1 (de)	1998-05-14	Verfahren zur Herstellung von aktivierten Killer-Monozyten und Verfahren zur Überprüfung ihrer tumoriziden Aktivität
DE3751605D1 (de)	1996-01-04	Therapeutisches Blutprodukt, Mittel und Verfahren zu dessen Erzeugung.
EP0536564B1 (de)	1997-07-16	Verfahren zur Reinigung von Streptolysin 0, intaktes Streptolysin 0 erhältlich nach diesem Verfahren und seine Verwendung