AR020331A1 - Construccion, y metodo para producir una proteina en una celula de planta - Google Patents

Construccion, y metodo para producir una proteina en una celula de planta

Info

Publication number
AR020331A1
AR020331A1 ARP990103391A ARP990103391A AR020331A1 AR 020331 A1 AR020331 A1 AR 020331A1 AR P990103391 A ARP990103391 A AR P990103391A AR P990103391 A ARP990103391 A AR P990103391A AR 020331 A1 AR020331 A1 AR 020331A1
Authority
AR
Argentina
Prior art keywords
peptide
plant
eukaryotic
plasto
protein
Prior art date
Application number
ARP990103391A
Other languages
English (en)
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of AR020331A1 publication Critical patent/AR020331A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Construccion que comprende los siguientes componentes en la direccion 5 a 3 de transcripcion: a) un promotor funcional en un plasto de planta; b) unasecuencia de ADN que codifica un péptido derivado de un organismo eucariotico; y c) una region de terminacion de transcripcion; en donde dicho péptidoeucariotico no es un péptido de un plasto de planta. La secuencia b) codifica para unpéptido nuclear de planta, un péptido antifungal o un péptido de mamíferodonde dicho péptido de mamífero es una proteína seleccionada entre el grupo que consiste en interferones, anticuerpos monoclonales, hormonas, agentehematopoyético, proteína de coagulacion no enzimática o inhibidor de proteinasa. Otros elementos incluyen un marcador seleccionable para la seleccion decélulas de plantas que comprenden un plasto que expresa el marcador y regiones de ADN de homología al genoma del plasto, y opcionalmente un sitio de enlaceribosomático unido al promotor. Métodos para expresar péptidos derivados de organismoseucarioticos en plastos de plantas. Con los métodos que utilizan dichasconstrucciones se producen altos niveles de péptidos eucarioticos, tales como proteínas mamíferas, en una célula de planta desarrollando células de plantasbajo condiciones por lascuales las secuencias codificadoras de ADN son expresadas para producir péptido eucariotico en dicho plasto. Método para producir unaproteína en una célula de planta mediante transformacion de dicha construccion.
ARP990103391A 1998-07-10 1999-07-12 Construccion, y metodo para producir una proteina en una celula de planta AR020331A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/113,244 US6512162B2 (en) 1998-07-10 1998-07-10 Expression of eukaryotic peptides in plant plastids

Publications (1)

Publication Number Publication Date
AR020331A1 true AR020331A1 (es) 2002-05-08

Family

ID=22348363

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990103391A AR020331A1 (es) 1998-07-10 1999-07-12 Construccion, y metodo para producir una proteina en una celula de planta

Country Status (2)

Country Link
US (1) US6512162B2 (es)
AR (1) AR020331A1 (es)

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US20100251425A9 (en) * 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
US20020174453A1 (en) * 2001-04-18 2002-11-21 Henry Daniell Production of antibodies in transgenic plastids
AU2003253781A1 (en) * 2002-07-03 2004-01-23 University Of Central Florida Expression of human interferon in transgenic chloroplasts
US20060128615A1 (en) * 2002-09-18 2006-06-15 Pierrette Gaudreau Ghrh analogues
WO2005005643A2 (en) * 2003-07-01 2005-01-20 Biolex, Inc. Chloroplast transformation of duckweed
US20060053516A1 (en) * 2003-12-05 2006-03-09 The University Of Hong Kong Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS
US20070037230A1 (en) * 2005-04-29 2007-02-15 Petrenko Valery A Peptides for selectively and specifically binding Bacillus anthracis spores and use thereof in landscape phages
JP5086999B2 (ja) 2005-09-16 2012-11-28 バイエル・クロップサイエンス・アーゲー 内腔指向性タンパク質を発現する色素体形質転換植物
JP5710978B2 (ja) 2007-11-08 2015-04-30 ネオジェニックス オンコロジー， インコーポレイテッド 結腸癌および膵臓癌に対する組み換えモノクローナル抗体および対応抗原
WO2011057243A2 (en) * 2009-11-09 2011-05-12 University Of Central Florida Research Foundation, Inc. Administration of plant expressed oral tolerance agents
US20150366890A1 (en) 2013-02-25 2015-12-24 Trustees Of Boston University Compositions and methods for treating fungal infections
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US9803017B2 (en) 2013-07-05 2017-10-31 University Of Washington Through Its Center For Commercialization Soluble MIC neutralizing monoclonal antibody for treating cancer
CN109456398A (zh) * 2017-09-06 2019-03-12 山东益华生物科技有限公司 植物作为宿主在表达表皮生长因子中的应用
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CN121194993A (zh) 2023-05-24 2025-12-23 莫扎特治疗公司 Cd8特异性结合蛋白及其应用方法
WO2025072406A1 (en) 2023-09-26 2025-04-03 Profoundbio Us Co. Ptk7 binding agents, conjugates thereof and methods of using the same
TW202547866A (zh) 2024-01-10 2025-12-16 丹麥商珍美寶股份有限公司 Ｓｌｉｔｒｋ６結合劑、其共軛物及使用彼之方法
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Also Published As

Publication number Publication date
US20020053094A1 (en) 2002-05-02
US6512162B2 (en) 2003-01-28

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