AR031336A1 - Metodos para la produccion a gran escala de proteinas en procariotas - Google Patents

Metodos para la produccion a gran escala de proteinas en procariotas

Info

Publication number
AR031336A1
AR031336A1 ARP010105300A ARP010105300A AR031336A1 AR 031336 A1 AR031336 A1 AR 031336A1 AR P010105300 A ARP010105300 A AR P010105300A AR P010105300 A ARP010105300 A AR P010105300A AR 031336 A1 AR031336 A1 AR 031336A1
Authority
AR
Argentina
Prior art keywords
methods
procariots
proteins
large scale
heterologous protein
Prior art date
Application number
ARP010105300A
Other languages
English (en)
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of AR031336A1 publication Critical patent/AR031336A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Métodos para la produccion de una proteína heterologa que se deriva de un ADN recombinante en células procarioticas, en el que dicha proteína heterologa se segrega extracelularmente como una proteína activa y correctamente plegada, y la célula procariotica contiene y expresa un vector que comprende el ADN que codifica dicha proteína heterologa, unido operativamente con el ADN que codifica el péptido de senal OmpA.
ARP010105300A 2000-11-14 2001-11-14 Metodos para la produccion a gran escala de proteinas en procariotas AR031336A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0027782.2A GB0027782D0 (en) 2000-11-14 2000-11-14 Methods fro large scale protein productio in prokaryotes

Publications (1)

Publication Number Publication Date
AR031336A1 true AR031336A1 (es) 2003-09-17

Family

ID=9903155

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010105300A AR031336A1 (es) 2000-11-14 2001-11-14 Metodos para la produccion a gran escala de proteinas en procariotas

Country Status (17)

Country Link
EP (1) EP1343909B1 (es)
JP (1) JP2004513648A (es)
KR (1) KR100857402B1 (es)
CN (1) CN100457909C (es)
AR (1) AR031336A1 (es)
AT (1) ATE440145T1 (es)
AU (1) AU2002221824A1 (es)
CA (1) CA2428643A1 (es)
CY (1) CY1110546T1 (es)
DE (1) DE60139635D1 (es)
DK (1) DK1343909T3 (es)
ES (1) ES2332124T3 (es)
GB (1) GB0027782D0 (es)
MX (1) MXPA03004162A (es)
PT (1) PT1343909E (es)
UY (1) UY27021A1 (es)
WO (1) WO2002040696A2 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0027779D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods for large scale production of recombinant dna-derived tpa or k2s molecules
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
JP5714230B2 (ja) 2007-01-31 2015-05-07 フェネックス インコーポレイテッド 発現上昇のための細菌リーダー配列
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
JP5444553B2 (ja) 2007-04-27 2014-03-19 フェネックス インコーポレイテッド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
CN102770555B (zh) * 2009-11-17 2015-01-28 詹森生物科技公司 改善的细菌膜蛋白分泌
CN102924602B (zh) * 2011-08-08 2015-06-17 深圳市艾派格生物技术有限公司 金离子吸附蛋白表面展示系统及富集并回收金离子的方法
CN106188311B (zh) * 2016-07-18 2020-08-04 山东大学齐鲁医院 一种重组肿瘤坏死因子相关细胞凋亡诱导配体蛋白的制备方法与应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
JPH05219963A (ja) * 1990-07-18 1993-08-31 Applied Immune Sci Inc 温度誘導分泌発現ベクターおよびその原核細胞での使用
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen

Also Published As

Publication number Publication date
JP2004513648A (ja) 2004-05-13
EP1343909B1 (en) 2009-08-19
ES2332124T3 (es) 2010-01-27
WO2002040696A2 (en) 2002-05-23
ATE440145T1 (de) 2009-09-15
CN100457909C (zh) 2009-02-04
KR20030051821A (ko) 2003-06-25
CN1628176A (zh) 2005-06-15
MXPA03004162A (es) 2004-04-20
DE60139635D1 (de) 2009-10-01
CA2428643A1 (en) 2002-05-23
WO2002040696A3 (en) 2002-09-19
EP1343909A2 (en) 2003-09-17
HK1077848A1 (zh) 2006-02-24
DK1343909T3 (da) 2009-12-21
GB0027782D0 (en) 2000-12-27
UY27021A1 (es) 2002-07-31
CY1110546T1 (el) 2015-04-29
KR100857402B1 (ko) 2008-09-08
PT1343909E (pt) 2009-09-11
AU2002221824A1 (en) 2002-05-27

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