ATE317443T1 - N-terminal verlängerte proteine exprimiert in hefe - Google Patents

N-terminal verlängerte proteine exprimiert in hefe

Info

Publication number
ATE317443T1
ATE317443T1 AT97948751T AT97948751T ATE317443T1 AT E317443 T1 ATE317443 T1 AT E317443T1 AT 97948751 T AT97948751 T AT 97948751T AT 97948751 T AT97948751 T AT 97948751T AT E317443 T1 ATE317443 T1 AT E317443T1
Authority
AT
Austria
Prior art keywords
yeast
polypeptide
dna
eegepk
sequence encoding
Prior art date
Application number
AT97948751T
Other languages
English (en)
Inventor
Thomas Börglum Kjeldsen
Svend Havelund
Annette Frost Pettersson
Per Balschmidt
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE317443T1 publication Critical patent/ATE317443T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT97948751T 1996-12-20 1997-12-18 N-terminal verlängerte proteine exprimiert in hefe ATE317443T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK148296 1996-12-20

Publications (1)

Publication Number Publication Date
ATE317443T1 true ATE317443T1 (de) 2006-02-15

Family

ID=8105307

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97948751T ATE317443T1 (de) 1996-12-20 1997-12-18 N-terminal verlängerte proteine exprimiert in hefe

Country Status (7)

Country Link
EP (1) EP0946735B1 (de)
JP (1) JP4187796B2 (de)
AT (1) ATE317443T1 (de)
AU (1) AU7873798A (de)
DE (1) DE69735242T2 (de)
ES (1) ES2258797T3 (de)
WO (1) WO1998028429A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999037793A1 (en) 1998-01-23 1999-07-29 Novo Nordisk A/S Process for making desired polypeptides in yeast
JP4261195B2 (ja) * 2001-04-24 2009-04-30 イノジェネティックス・ナムローゼ・フェンノートシャップ 酵母のコア−グリコシル化されたｈｃｖエンベロープタンパク質の発現
WO2002100887A2 (en) * 2001-06-08 2002-12-19 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
JP4386722B2 (ja) * 2001-07-24 2009-12-16 ノボ ノルディスク アクティーゼルスカブ アシル化されたポリペプチドの製造方法
GB201008682D0 (en) 2010-05-25 2010-07-07 Vib Vzw Epitope tag for affinity based applications
CN107743494B (zh) 2015-06-02 2022-04-29 诺和诺德股份有限公司 具有极性重组延伸体的胰岛素
AR106364A1 (es) 2015-08-25 2018-01-10 Novo Nordisk As Derivados de insulina y sus usos médicos
US20190010206A1 (en) 2015-08-25 2019-01-10 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
US20180244743A1 (en) 2015-08-25 2018-08-30 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
TW201917134A (zh) 2017-08-17 2019-05-01 丹麥商諾佛 儂迪克股份有限公司 新穎的醯基化胰島素類似物及其用途
US20210032307A1 (en) * 2018-02-09 2021-02-04 Jiangsu Hengrui Medicine Co., Ltd. Codon optimized precursor gene and signal peptide gene of human insulin analogue
DK4073097T3 (da) 2019-12-11 2024-08-19 Novo Nordisk As Nye insulinanaloger og anvendelser heraf

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
IL114160A (en) * 1994-06-17 2006-12-31 Novo Nordisk As Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast

Also Published As

Publication number Publication date
ES2258797T3 (es) 2006-09-01
WO1998028429A1 (en) 1998-07-02
EP0946735B1 (de) 2006-02-08
JP4187796B2 (ja) 2008-11-26
AU7873798A (en) 1998-07-17
EP0946735A1 (de) 1999-10-06
DE69735242D1 (de) 2006-04-20
DE69735242T2 (de) 2006-09-21
JP2001507574A (ja) 2001-06-12

Similar Documents

Publication Publication Date Title
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
IL184112A0 (en) A method for producing polypeptides or proteins in a disulfide-bridged two-chain form
NO963225D0 (no) Multivariant IL-3 hemattopoiese fusjonsprotein
DE69735242D1 (de) N-terminal verlängerte proteine exprimiert in hefe
EP0150126A3 (en) The synthesis of protein with an identification peptide
TR199900624T2 (xx) Proteinlerin y�ksek seviyede ekspresyonu.
ATE160587T1 (de) Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung
ATE121454T1 (de) Ubiquitinspezifische protease.
DE69634757D1 (de) Vektor zur expression von n-terminal verlängerten proteinen in hefezellen
EP0195680A3 (de) Proteinsynthese mit einem Identifizierungspeptid
TR200200048T2 (tr) Otoproteolitik klevaj vasıtasıyla proteinlerin üretimi.
WO1999002708A8 (en) Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein
EP0750040A3 (de) Varianten des Makrophagen-stimulierendes-Proteins und Verfahren zur ihrer Herstellung
AU1541395A (en) Folding proteins
TR199802168T2 (xx) IL-16 Aktivitesine sahip işlenmiş polipeptidler, bunları üretmeye mahsus yöntemler ve bunların kullanımı.
WO2004029209A3 (en) Epitope-tagged recombinant growth arrest specific gene 6 protein
ES2146666T3 (es) Procedimiento para la obtencion recombinante de proteinas en levadura.
RU92004585A (ru) Промотор, днк-последовательность, вектор, клетки, способ экспрессии днк
Ovchinnikov et al. Complete primary structure of cytoplasmatic aspartate-amino transferase from hog heart muscle

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties