AR086684A1 - Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado - Google Patents

Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado

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Publication number
AR086684A1
AR086684A1 ARP120101994A ARP120101994A AR086684A1 AR 086684 A1 AR086684 A1 AR 086684A1 AR P120101994 A ARP120101994 A AR P120101994A AR P120101994 A ARP120101994 A AR P120101994A AR 086684 A1 AR086684 A1 AR 086684A1
Authority
AR
Argentina
Prior art keywords
plant
genome
modification
cell
predefined site
Prior art date
Application number
ARP120101994A
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English (en)
Inventor
Katelijn Dhalluin
Original Assignee
Bayer Cropscience Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Cropscience Nv filed Critical Bayer Cropscience Nv
Publication of AR086684A1 publication Critical patent/AR086684A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Reivindicación 1: Un método para modificar el genoma de una célula vegetal de algodón en un sitio predefinido, caracterizado porque comprende los pasos de a) inducir la ruptura de ADN de cadena doble en la cercanía o en dicho sitio predefinido, donde dicha ruptura de cadena doble es inducida por la introducción en dicha célula de una enzima endonucleasa de clivaje raro que reconoce una secuencia de reconocimiento en la cercanía de o en dicho sitio predefinido; b) seleccionar una célula vegetal en donde dicha ruptura de ADN de cadena doble ha sido reparada dando como resultado una modificación en el genoma en dicho sitio preseleccionado, donde dicha modificación se selecciona entre i) un reemplazo de al menos un nucleótido; ii) la supresión de al menos un nucleótido; iii) una inserción de al menos un nucleótido; o iv) cualquier combinación de (i) - (iii); que se caracteriza porque dicha célula está comprendida dentro de los callos embrionarios.Reivindicación 20: El método de acuerdo con reivindicación 19, caracterizado porque dicho gen de interés que se puede expresar en plantas se selecciona del grupo de un gen de tolerancia a herbicidas, un gen de resistencia a insectos, un gen de resistencia a enfermedades, un gen de resistencia al estrés abiótico, una enzima relacionada con la biosíntesis de aceite, la biosíntesis de carbohidratos, una enzima relacionada con la fuerza de las fibras o con la longitud de las fibras, una enzima relacionada con la biosíntesis de metabolitos secundarios. Reivindicación 26: Una planta o fibra o semilla de de algodón o un material propagador de la misma, caracterizada porque comprende una modificación en un sitio predefinido del genoma, obtenida mediante el método de acuerdo con la reivindicación 25 o que consiste esencialmente de las células vegetales de algodón de la reivindicación 25. Reivindicación 28: Un método para producir una planta que comprende una modificación en un sitio predefinido del genoma, caracterizado porque comprende el paso de cruzar una planta que consiste esencialmente de las células vegetales de la reivindicación 25 o una planta de acuerdo con la reivindicación 26 con otra planta o consigo misma y opcionalmente cosechar las semillas.
ARP120101994A 2011-06-06 2012-06-06 Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado AR086684A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161493579P 2011-06-06 2011-06-06
EP11004570 2011-06-06

Publications (1)

Publication Number Publication Date
AR086684A1 true AR086684A1 (es) 2014-01-15

Family

ID=47295507

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120101994A AR086684A1 (es) 2011-06-06 2012-06-06 Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado

Country Status (10)

Country Link
US (2) US20140173770A1 (es)
EP (1) EP2718443B1 (es)
CN (1) CN103597082B (es)
AR (1) AR086684A1 (es)
AU (1) AU2012266597B2 (es)
ES (1) ES2657825T3 (es)
MX (1) MX348785B (es)
TR (1) TR201802544T4 (es)
WO (1) WO2012168124A1 (es)
ZA (1) ZA201308555B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109504700A (zh) * 2010-06-09 2019-03-22 拜尔作物科学公司 植物基因组改造中常用的在核苷酸序列上修饰植物基因组的方法和工具
CN104245940A (zh) * 2012-04-23 2014-12-24 拜尔作物科学公司 植物中的靶向基因组工程
CA2908403A1 (en) * 2013-04-02 2014-10-09 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
CN104862302B (zh) * 2015-05-05 2020-12-15 华南师范大学 一种dna片段化的方法及实现该方法的装置
EP3508581A1 (en) 2018-01-03 2019-07-10 Kws Saat Se Regeneration of genetically modified plants
EP3708651A1 (en) 2019-03-12 2020-09-16 KWS SAAT SE & Co. KGaA Improving plant regeneration
EP3757219A1 (en) * 2019-06-28 2020-12-30 KWS SAAT SE & Co. KGaA Enhanced plant regeneration and transformation by using grf1 booster gene

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