ATE106449T1 - Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen. - Google Patents

Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.

Info

Publication number: ATE106449T1
Authority: AT; Austria
Prior art keywords: amino acid; protein; terminal; type; regulator
Prior art date: 1989-06-30

Application number

AT90911196T

Other languages

English (en)

Inventor

Rohan T Baker

David K Gonda

Alexander Varshavsky

Original Assignee

Massachusetts Inst Technology

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-06-30

Filing date

1990-06-27

Publication date

1994-06-15

1990-06-27 Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology

1994-06-15 Application granted granted Critical

1994-06-15 Publication of ATE106449T1 publication Critical patent/ATE106449T1/de

Links

230000005764 inhibitory process Effects 0.000 title 1
230000037361 pathway Effects 0.000 title 1
102000004169 proteins and genes Human genes 0.000 abstract 9
108090000623 proteins and genes Proteins 0.000 abstract 9
150000001413 amino acids Chemical class 0.000 abstract 4
108010016626 Dipeptides Proteins 0.000 abstract 3
125000000729 N-terminal amino-acid group Chemical group 0.000 abstract 3
230000003834 intracellular effect Effects 0.000 abstract 3
229920001184 polypeptide Polymers 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
210000004027 cell Anatomy 0.000 abstract 2
230000001965 increasing effect Effects 0.000 abstract 2
241000024188 Andala Species 0.000 abstract 1
230000002159 abnormal effect Effects 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1
125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
230000015556 catabolic process Effects 0.000 abstract 1
201000010099 disease Diseases 0.000 abstract 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
230000002708 enhancing effect Effects 0.000 abstract 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 abstract 1
210000003527 eukaryotic cell Anatomy 0.000 abstract 1
238000001727 in vivo Methods 0.000 abstract 1
125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 abstract 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Mycology (AREA)
Medicinal Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Pharmacology & Pharmacy (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Compounds Of Unknown Constitution (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Fats And Perfumes (AREA)

AT90911196T 1989-06-30 1990-06-27 Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen. ATE106449T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US37382589A	1989-06-30	1989-06-30

Publications (1)

Publication Number	Publication Date
ATE106449T1 true ATE106449T1 (de)	1994-06-15

Family

ID=23474039

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT90911196T ATE106449T1 (de)	1989-06-30	1990-06-27	Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.

Country Status (10)

Country	Link
US (1)	US5766927A (de)
EP (1)	EP0479912B1 (de)
JP (1)	JP2904921B2 (de)
AT (1)	ATE106449T1 (de)
AU (1)	AU5963290A (de)
CA (1)	CA2063400C (de)
DE (1)	DE69009476T2 (de)
DK (1)	DK0479912T3 (de)
ES (1)	ES2054365T3 (de)
WO (1)	WO1991000356A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5861312A (en) *	1997-12-02	1999-01-19	California Institute Of Technology	Nucleic acid encoding mammalian UBR1
AU2605600A (en) *	1999-01-11	2000-08-01	Mindset Biopharmaceuticals (Usa) Inc.	Methods for regulating the stability of recombinant proteins and products usefultherein
US6306663B1 (en)	1999-02-12	2001-10-23	Proteinex, Inc.	Controlling protein levels in eucaryotic organisms
WO2000050089A2 (en) *	1999-02-26	2000-08-31	Mindset Biopharmaceuticals (Usa) Ltd.	Regulation of the stability of recombinant proteins and antiboodies
US7262005B1 (en)	2000-02-04	2007-08-28	Aurora Biosciences Corporation	Methods of protein destabilization and uses thereof
KR101455222B1 (ko)	2002-12-16	2014-10-31	글로브이뮨	면역 요법으로서의 효모계 백신
US8343502B2 (en)	2002-12-16	2013-01-01	Globeimmune, Inc.	Yeast-based vaccines as immunotherapy
WO2008039483A2 (en) *	2006-09-26	2008-04-03	Massachusetts Institute Of Technology	Modified self-assembling peptides
US8173792B2 (en)	2007-02-09	2012-05-08	The Board Of Trustees Of The Leland Stanford Junior University	Method for regulating protein function in cells using synthetic small molecules
US8530636B2 (en)	2008-05-07	2013-09-10	The Board Of Trustees Of The Leland Stanford Junior University	Method for regulating protein function in cells in vivo using synthetic small molecules
US8999896B2 (en) *	2010-01-15	2015-04-07	California Institute Of Technology	Discovery and applications of the proteolytic function of N-terminal acetylation of cellular proteins
NZ727213A (en)	2012-03-09	2020-03-27	Vestaron Corp	Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11446398B2 (en)	2016-04-11	2022-09-20	Obsidian Therapeutics, Inc.	Regulated biocircuit systems
JP2023522947A (ja)	2020-04-20	2023-06-01	ヴェスタロン・コーポレーション	タンパク質分解に対して安定な害虫制御用ｕ１－アガトキシン－ｔａ１ｂバリアントポリペプチド
IL297738A (en)	2020-05-01	2022-12-01	Vestaron Corp	Insecticidal combinations
US20250188136A1 (en)	2021-04-01	2025-06-12	Vestaron Corporation	Av3 mutant polypeptides for pest control
IL313184A (en)	2021-11-29	2024-07-01	Ironwood Pharmaceuticals Inc	Pharmaceutical compositions for the treatment of visceral pain
WO2023122805A1 (en)	2021-12-20	2023-06-29	Vestaron Corporation	Sorbitol driven selection pressure method
US20260013513A1 (en)	2022-05-18	2026-01-15	Vestaron Corporation	Pesticidal actx peptide variants
WO2023245100A1 (en)	2022-06-17	2023-12-21	Vestaron Corporation	Antimicrobial ncr13 variant peptides
WO2024026406A2 (en)	2022-07-29	2024-02-01	Vestaron Corporation	Next Generation ACTX Peptides
WO2025226701A1 (en)	2024-04-23	2025-10-30	Vestaron Corporation	Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control
WO2026050206A1 (en)	2024-08-27	2026-03-05	Ironwood Pharmaceuticals, Inc.	Combinations of glp-1 and guanylate cyclase c (gc-c) receptor agonists

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4512979A (en) *	1981-03-23	1985-04-23	Merck & Co., Inc.	Dipeptides containing thialysine and related amino acids as antihypertensives
US4514391A (en) *	1983-07-21	1985-04-30	E. R. Squibb & Sons, Inc.	Hydroxy substituted peptide compounds
US4551445A (en) *	1983-10-14	1985-11-05	The Medical College Of Ohio	Derivatives of arginine vasopressin antagonists
ATE247708T1 (de) *	1986-10-02	2003-09-15	Massachusetts Inst Technology	Verfahren zur regulierung der metabolischen stabilität von proteinen
US5132213A (en) *	1986-10-02	1992-07-21	Massachusetts Institute Of Technology	Method for producing proteins and polypeptides using ubiquitin fusions
US5108919A (en) *	1988-06-24	1992-04-28	Genentech, Inc.	Dna sequences encoding yeast ubiquitin hydrolase
CH680013A5 (de) *	1988-09-19	1992-05-29	Mathias Och

1990
- 1990-06-27 DE DE69009476T patent/DE69009476T2/de not_active Expired - Fee Related
- 1990-06-27 CA CA002063400A patent/CA2063400C/en not_active Expired - Fee Related
- 1990-06-27 ES ES90911196T patent/ES2054365T3/es not_active Expired - Lifetime
- 1990-06-27 WO PCT/US1990/003669 patent/WO1991000356A1/en not_active Ceased
- 1990-06-27 DK DK90911196.5T patent/DK0479912T3/da active
- 1990-06-27 AU AU59632/90A patent/AU5963290A/en not_active Abandoned
- 1990-06-27 JP JP2510142A patent/JP2904921B2/ja not_active Expired - Fee Related
- 1990-06-27 EP EP90911196A patent/EP0479912B1/de not_active Expired - Lifetime
- 1990-06-27 AT AT90911196T patent/ATE106449T1/de not_active IP Right Cessation
1994
- 1994-04-20 US US08/230,453 patent/US5766927A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
AU5963290A (en)	1991-01-17
JPH04506298A (ja)	1992-11-05
DE69009476D1 (de)	1994-07-07
US5766927A (en)	1998-06-16
EP0479912B1 (de)	1994-06-01
DK0479912T3 (da)	1994-10-10
ES2054365T3 (es)	1994-08-01
WO1991000356A1 (en)	1991-01-10
CA2063400A1 (en)	1990-12-31
DE69009476T2 (de)	1994-12-22
EP0479912A1 (de)	1992-04-15
JP2904921B2 (ja)	1999-06-14
CA2063400C (en)	1997-07-15

Legal Events

Date	Code	Title	Description
1995-03-15	UEP	Publication of translation of european patent specification
2006-03-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE106449T1 (de)	1994-06-15	Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.
Reed et al.	1980	Sequence of residues 400--403 of bovine serum albumin
Gregory et al.	1977	The primary structure of human urogastrone
ES2063350T3 (es)	1995-01-01	Nuevas proteinas con efecto inhibidor del tnf y su produccion.
Cameron et al.	1985	Amino acid sequence analysis of human interleukin 1 (IL-1). Evidence for biochemically distinct forms of IL-1.
ATE24919T1 (de)	1987-01-15	Menschliches pankreatisches grf.
KR850008681A (ko)	1985-12-21	합성펩티드의 제조방법
ATE171712T1 (de)	1998-10-15	Neuartiger menschlicher proteaseinhibitor vom kunitz-typ und entsprechende varianten
DE3486154D1 (de)	1993-07-01	Cardiodilatin, ein neues peptidhormon und verfahren zu seiner herstellung.
DE3261304D1 (en)	1985-01-03	Retro-inverso analogues of c-terminal penta and hexapeptides of substance p.
Ramage et al.	1989	Solid phase peptide synthesis of ubiquitin
ABEL et al.	1971	Structural differences in the hinge region of human gamma A myeloma proteins of different subclasses
DK0446315T3 (da)	1998-01-05	Fremgangsmåde til fremstilling af PAI-2
Brandt et al.	1984	Complete amino acid sequence of the N-terminal extension of calf skin type III procollagen
Hackeng et al.	1998	Chemical synthesis of human protein S thrombin‐sensitive module and first epidermal growth factor module
NO20016341L (no)	2002-02-25	Inhibitorer for integrinet <alfa>v<beta>6
AU2676399A (en)	1999-08-30	Novel cystine knot protein and materials and methods for making it
Liebisch et al.	1986	Isolation and structure of a C-terminally amidated nonopioid peptide, amidorphin-(8-26), from bovine striatum: a major product of proenkephalin in brain but not in adrenal medulla.
Milstein et al.	1969	Occurrence of the Inv genetic markers in the subgroups of human kappa chains
DE60326726D1 (de)	2009-04-30	Peptide mit affinität für ein phospholipid und deren verwendung
Harris et al.	1996	Species differences in insulin.
RU94036002A (ru)	1996-09-27	Ингибитор протеазы, днк, вектор, клетка, способ получения ингибитора, фармацевтическая композиция
RU94036770A (ru)	1996-09-27	Домен ингибитора протеазы, днк, вектор, клетка, способ получения домена, фармацевтическая композиция, применение домена
TH54460A3 (th)	2002-12-17	สารผสมฟลินท์ (flint) และการใช้ของสารผสม
TH12889A (th)	1993-07-10	เคอร์คูลิน บี และคีเอ็นเอที่เป็นรหัสของสารดังกล่าว และขบวนการสำหรับการผลิตสารดังกล่าว